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The authors have no financial interest in this presentation
Iladevi Cataract and IOL Research Centre, Ahmedabad, India
Shail A. Vasavada, DO
Devarshi Gajjar, PhD
Kaid Johar SR, PhD
Abhay Vasavada, MS, FRCS (England)
Failure in regression of hyaloid vasculature results in a
pathological condition called Persistant Fetal Vasculature
(PFV)
Arresten:α1 chain COLIV
Canstatin:α2 chain COLIV
Tumstatin: α3 chain COLIV
Endostatin: α1 chain COLXVIII
1Sottile J. Biochem BiophysActa. 20044;1654(1):13-22.
2Mundel TM, Kalluri R. Microvasc Res. 2007;74(2-3):85-9.
Arresten:α1 chain COLIV
Canstatin:α2 chain COLIV
Tumstatin: α3 chain COLIV
Endostatin: α1 chain COLXVIII
1Sottile J. Biochem BiophysActa. 20044;1654(1):13-22.
2Mundel TM, Kalluri R. Microvasc Res. 2007;74(2-3):85-9.
Regulation of Angiogenesis Collagen Derived Collagen Derived AAFsAAFs
Embryonic Stage : Lens covered by tunica vasculosa
lentis
However the exact mechanism of angiogenesis and regression is
not completely known
Study Design Case-Control Study
Cases – Pediatric eyes with anterior PFV (n=6)
Controls – Age-matched Congenital cataracts (n=6)
Purpose To measure mRNA levels of
Antiangiogenic factors (arresten, canstatin, tumstatin, endostatin) and
Matrix Metalloproteinases (MMP-2 and MMP-9) in lens epithelial cells(LECs) of
eyes with and without PFV
* All the anterior capsules collected from pediatric eyes during the surgery were in accordance with Declaration of Helsinki. Ethical
approval for the study and use of human LECs was obtained from the Institutional Ethics Committee
Material and Methods
Representative PFV Cases: membranous cataract, traction on ciliary processes, fibrovascular plaque
Amplification curves
Non-parametric Mann-Whitney U test
performed
Melting peaks
Melting curves
Amplification curves
Standardization for qRT-PCR
•Quantitative real time RT-PCR performed to determine the mRNA levels of arresten, canstatin, tumstatin, endostatin, MMP-2, MMP-9, GAPDH and Beta actin (Housekeeping genes)
*
*P=0.01, # P=0.02,
Relative mRNA expression of AAFs and MMPs in PFV cases and ControlsIn eyes with
PFV...
The mRNA level of MMP-2 was 4 times higher in PFV cases
No change was observed in MMP-9 mRNA levels (p = 0.82)
Results
AAFs
Arresten 1.6 times
Tumstatin 2.5 times
Canstatin 2.3 times
#
*
3Zhu M et al., Exp Eye Res. 2000;70(6):767-76 4Duh EJ et al., Ophthalmology. 2004; 111:1885-85Saint-Geniez M and D’Amore PA Int J Dev Biol 2004; 48:1045–1058 6Hackett SF et al., J Cell Physiol. 2002;192:182–187.7Reichel MB et al., Cell Death Differ. 1998;5:156 –162. 8Thornton JD et al., Invest Ophthalmol Vis Sci. 2007 ;48:491-99Rutland CS et al., Mol Vis.2007;19:47-56. 10Gehlbach P et al., Neuroscience. 2006;137(2):447-61.
Speculation•In the present study we speculate that increased level
of canstatin and tumstatin could be to cope up with increased angiogenesis in the pathological condition of
PFV•Decreased levels of Arresten could be because of the developmental shift in collagen IV of the lens capsule
First study on human PHPV eyes
Future Direction Further studies on the protein levels, growth
factors and other inhibitors
Conclusion
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