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Alleviation of chilling injury in mangoes using

cryoprotectants

Aim

The objective of this study is to verify the feasibility of using cryoprotectants (propylene glycol, glycerol, and sorbitol) during the storage of mangoes under low temperatures.

Introduction

Mango is a climacteric fruit with limited shelf-life, both in ambient conditions (25°C) and under low temperatures ( 50% of the skin surface); 3. mild (up to 25% of the skin surface); 2. less than mild (2-5% of skin surface), and 1. without CI symptoms(3)

Lipid peroxidation: Skin tissue (0,5 g) was ground using liquid nitrogen and the dry powder mixed with 0.1% trichloroacetic acid (TCA) and centrifuged (3000 x g) at 4°C for 20 minutes (Gratão et al., 2012). The results were expressed as ηmol of MDA.g-1.MF.

Experimental design and data analysis

The experiment was set according to a completely randomized design in a 4 (control and three cryoprotectants) x 3 (doses) x 5 (withdraws – 0, 7, 14, 21, and 28 days) factorial arrangement with 5 repetitions of one fruit. The data were subject to analysis of variance (ANOVA) using AgroEstat and the comparisons of means of transformed data were based on Tukey’s test at p = 0.05 level of significance.

References

1Mitra, S.K. and Baldwin, E.A. 1997. Mango, in: Mitra S.K.(Ed), Postharvest physiology and storage of tropical and subtropical fruits. CAB international, New York, NY. 85-77. 2MacDonald, G. A.; Lanier, T. C. Cryoprotectants for improving frozen-food quality. In: Erickson M.C., Hung YC. (eds) Quality in Frozen Food. Springer, Boston, MA, 36 p. 1997. 3Miguel, A. C. A. et al. Injúria pelo frio na qualidade pós-colheita de mangas cv. Palmer. Revista Brasileira de Fruticultura, 1:255-260, 2011. 4Gratão, P. L., et al. Biochemical dissection of diageotropica and Never ripe tomato mutants to Cd-stressful conditions. Plant Physiology and Biochemistry, 56:79-96, 2012.

Acknowledgments

AD

abC

bB

abA

0,0

1,0

2,0

3,0

4,0

5,0

6,0

7,0

0 7 14 21 28

LP (

nM

ol M

DA

g.F

M)

Storage period (days)

ControlGlycerolPropylene glycolSorbitol

Figure 2. Skin lipid peroxidation (ηMol MDA.g-1 FW) in ‘Palmer’ mangoes treated with different cryoprotectants during cold storage at 8.5 + 1.5℃ for 28 days.

0

1

2

3

4

0 7 14 21 28

CI s

ever

ity

(1 -

4)

Storage period (days)

Control

Glycerol

Propylene glycol

Sorbitol

Figure 1. Chilling injury severity (1 – 5) in ‘Palmer’ mangoes treated with different cryoprotectants during cold storage at 8.5 + 1.5℃ for 28 days.

Treatment application Storage

The 7th International Horticulture Research Conference