sod research 5/09
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SOD Research 5/09
- SOD Spore Surveys & SOD Blitz / Outreach
- Phosphonate Treatment of Tanoaks and CLOs
- Long Term Efficacy of SOD Treatments
- Resistance / Tolerance in Tanoaks
- Transmission of SOD Through Pruning
- Management Planning
SOD Spore MonitoringSOD Spore Monitoring
8 Field Monitoring Sites8 Field Monitoring Sites 16 Buckets / Site16 Buckets / Site 5 5 Leaves / BucketLeaves / Bucket = 640 leaves sampled every 3 weeks= 640 leaves sampled every 3 weeks Starting again in February 08Starting again in February 08
Bay Laurel / Tanoak SOD Spore Survey
Date
Temp (C)
Rain (mm)
SOD Blitz – Disease MonitoringSOD Blitz – Disease Monitoring
SOD Blitz / SOD Treatment Training Sessions
Topical TreatmentTopical TreatmentInjection TreatmentInjection Treatment
Save the Tanoaks ProjectSave the Tanoaks Project
SOD Treatment SitesSOD Treatment SitesLocation: Sites Plot Pairs TreesCarmel Valley 2 6 54
SDSF 2 4 883
LPNF 1 2 36
MPRPD 1 2 46
Homeowners 13 na 107
Long Term Efficacy of SOD Treatments
0
20
40
60
80
100
Time 0 3 Months 6 Months 1 Year 1.5 Year 2 Year 2.5 Year
Agrifos vs. Azomite TreatmentsC
anke
r Siz
e (%
of U
ntre
ated
Pos
itive
Con
trol)
Detached Branch Assay
Novato Mature CLOs
Agrifos & PentraBark
Azomite & Limewash
Long Term Efficacy of SOD Treatments
0
5
10
15
20
25
Large Medium
Tree Size Class
Les
ion
Size
(mm
) PCTopicalInjectNC
Effect of Tree Size on Treatment Efficacy
Tanoak Common Garden Experiment Shadehouse
Tanoak Out Planting – Common Garden Experiment
Tanoak Assays
Tanoak PetiolePlug Assay
UntreatedControl Tree
Phosphonate Treated Tree
Whole Seedling Inoculation
Infected Seedling
00
11
22
33
44
55
66
77
88
99
11 22 33 44 55 66 88 99 1111 1313 1414 1515 1616 1717 1818 1919 2121 2323 2424 2525 5050
FamilyFamily
Mea
n Le
sion
Len
gth,
Apr
09M
ean
Lesi
on L
engt
h, A
pr09
Whole-seedling inoculation
Zoospores are in Direct Contact with
Tree for 24 hrs.
Grafting Wax Creates a Water Tight Seal
Zoospore Inoculator for P. Ramorum
1 cm
Transmission of SOD through pruning:
Zoospore Inoculator
Pruning CLO Branches
► Does tree pruning introduce SOD infection?► Is the timing of cutting a factor?
Test at: 1 week 1 month 2 months 4 months
Grady Ranch Site
Transmission of SOD Through Pruning
01020304050607080
1 Week 1 Month 2 Months 4 Months
Time Post Pruning
Infe
cted
Bra
nche
s (%
)
Grady Ranch11/27/07
Transmission of SOD Through Pruning
Air Sampling for SOD Spores
Steam Baiting for SOD Monitoring
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