results glucose levels measured by analox were higher compare to handheld glucometer in type 1...
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Results• Glucose levels measured by Analox were higher compare to handheld glucometer in type 1 diabetic rats.
Results (cont)
Evaluation of Blood Glucose Levels Using a Handheld Glucometer as Compared to a Clinical Chemistry Analyzer in BBDP/Wor and BBZDR/Wor Rats
Barak Yahalom, Shane K. Duclos, Dennis L. Guberski, Christian W. Grant and Joan F. Flanagan
Biomedical Research Models, Inc., Worcester, MA
IntroductionHistorically, BRM’s facilities have been using a clinical chemistry analyzer (Analox) to determined serum glucose levels for our proprietary diabetic prone rat models. This method is an operationally time consuming process. Additionally, a whole blood glucose analysis via a handheld glucometer is a less expensive and faster method for evaluation of glycemic state. Portable glucometers have been developed and used for whole blood glucose analysis in human patients and are increasingly used to monitor diabetic animals. Moreover, their sensitivity and accuracy has been continuously improved. Therefore, we evaluated the accuracy of whole blood glucose levels measured by a handheld glucometer (Contour®) as compared to serum glucose levels (SGL) measured by Analox in BBDP/Wor and BBZDR/Wor rats. Cohorts of rats that exhibited chronic diabetes were used in this study. Type 1 diabetic BBDP/Wor rats were tested for glucose levels twice daily, prior and 3-4 hours post insulin administration. Type 2 diabetic BBZDR/Wor rats were tested once daily during the non-fed state. At each time point blood was measured by Analox and glucometer. Our data show that no statistically significant difference was detected between Analox or glucometer measurements in BBZDR/Wor rats. However, glucose levels measured by a handheld glucometer were significantly lower than the levels measured by Analox in BBDP/Wor rat. Moreover, when categorizing the readouts into subgroups according to SGL> 500 mg/dL, 200 - 500 mg/dL or < 200 mg/dL, glucose levels measured by a glucometer were statisitically significantly lower than the levels measured by Analox in all three subcategories. In summary, using a handheld glucometer for measuring blood glucose levels in BBZDR/Wor is equivalent to the use of a clinical chemistry analyzer. However, the BBDP/Wor rat’s poor hydration state may contribute to the higher glucose values measured by clinical chemistry analyzer in comparison to the values measured by a glucometer.
MethodsAnimal Models
• BBDP/Wor, and BBZDR/Wor as commonly
used models for Type 1 and Type 2 diabetes,
respectively.
• Chronic diabetic male rats with average
duration of 12 days for BBDP/Wor and 88 days
for BBZDR/Wor
Diagnosis of Diabetes
• Animals with a positive glycosuria and serum
glucose level of >250mg/dL were considered
diabetic
Insulin Treatment Protocol (BBDP only)
• PZI ® (protamine zinc insulin) was inwjected
once daily subcutaneously at 0.9U/100g BW
Glucose measurements
• Glucose levels were measured for 5 days by
handheld glucometer (Contour®) for whole
blood samples and Analox clinical chemistry
analyzer (Model GM9D, Leominster, MA) for
serum glucose levels (SGL)
• BBDP/Wor (n=12)
• Prior to and 3-4 hours post-insulin
administration (n=120 blood samples)
• BBZDR/Wor (n=12)
•Once a day at the AM (n=60 samples)
Figure 2: Mean Glucose Levels (mg/dL) For BBZDR/Wor Rats Tested by Different Methods (Mean + SD)
A) Glucose Measurements at the High End of the Linear Range for Handheld Glucometer (SGL ≥ 500 mg/dL)
Acknowledgements• This work was funded by BRM, and performed
by the Springfield facility technical staff.
• For our BBZDR/Wor (T2D) rat colony
management and experimental studies, using a
handheld glucometer for measuring whole blood
glucose levels is equivalent to the use of clinical
chemistry analyzer (Analox) for SGL.
•For our BBDP/Wor rats (T1D), using a handheld
glucometer is sufficient for colony management,
except for the low end of the linear range of the
handheld glucometer, which due to safety reasons,
additional test with clinical chemistry analyzer is
recommended.
• For study protocols which used BGL’s in T1D rats
as a study end point, a clinical chemistry analyzer is
recommended.
Conclusion
Figure 1: Mean Glucose Levels (mg/dL) For BBDP/Wor Rats Tested by Different Methods (Mean + SD)
B) Glucose Measurements at the Mid Range of the Linear Range for Handheld Glucometer (200 mg/dL < SGL < 500 mg/dL)
C) Glucose Measurements at the Low End of the Linear Range for Handheld Glucometer (SGL ≤ 200 mg/dL)
• Glucose levels measured by Analox were equivalent when compared to handheld glucometer in type 2 diabetic rats.
* Indicates statistical difference of p < 0.05 using T-test.
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