polyclonal antibody adiponectin - proteintechbackground information adiponectin (adipoq), an...

AdiponectinPolyclonal ANTIBODYCatalog Number:21613-1-AP 2 Publications

For Research Use Only

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Basic Information Catalog Number:21613-1-APSize:60 μg/150 μlSource:RabbitIsotype:IgGPurif ication Method:Antigen aff inity purif icationImmunogen Catalog Number:AG16304

GenBank Accession Number:BC096308GeneID (NCBI):9370Full Name:adiponectin, C1Q and collagen domaincontainingCalculated MW:244aa,26 kDaObserved MW:29 kDa

Recommended Dilutions:WB 1:200-1:1000 IHC 1:50-1:500 IF 1:50-1:500

Applications Tested Applications:IF, IHC, WB, ELISACited Applications:WBSpecies Specif icity:human, mouseCited Species:humanNote: suggested an gen retrieval with TEbuffer pH 9.0; (*) Alterna vely, an genretrieval may be performed with citratebuffer pH 6.0

Positive Controls:WB : NIH/3T3 cells; 3T3-L1 cellsIHC : human liver tissue; human skin tissueIF : NIH/3T3 cells;

Background Information Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number ofmetabolic processes, including improving insulin sensitivity and anti-inf lammatory activity. The role of AdipoQ in reproduction is not yet fully understood, butthe expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and humanplacenta. Adiponectin exerts its ef fects by activating a range of dif ferent signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cellgrowth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Notable Publications Author Pubmed ID Journal Application

Jinghui Jiang 30745818 Int J Biol Sci WB

Xuerong Sun 29288588 Cell Biol Int WB

Storage Storage:Store at -20ºC. Stable for one year af ter shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.Aliquoting is unnecessary for -20ºC storage

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Selected Validation Data

Immunohistochemistry of paraff in-embeddedhuman liver tissue slide using 21613-1-AP(ADIPOQ antibody) at dilution of 1:200 (under 10xlens).

Immunohistochemistry of paraff in-embeddedhuman liver tissue slide using 21613-1-AP(ADIPOQ antibody) at dilution of 1:200 (under 40xlens).

NIH/3T3 cells were subjected to SDS PAGEfollowed by western blot with 21613-1-AP(ADIPOQ Antibody) at dilution of 1:300 incubatedat room temperature for 1.5 hours

Immunofluorescent analysis of ( 4% PFA ) f ixedNIH/3T3 cells using 21613-1-AP(ADIPOQantibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Aff iniPure Goat Anti-Rabbit IgG(H+L)