mid-ir laser imaging for molecular recognition in security and medical applications

Mid-IR laser imaging for molecular recognition in

security and medical applications

Lasers, Optics & Photonics, Oct 7-9, 2013 InfraSign

Anadi Mukherjee

InfraSignFremont, CA

Mid-IR laser imaging for

Molecular Recognition

www.infrasign.com

Security HAZMATs Food Safety

Medical Tagless Pathology Pharma QC/QA

Hand-held, battery operated system

2 InfraSignLasers, Optics & Photonics, Oct 7-9, 2013

Revealing the Invisible for a Safer World

InfraSign

LaserCamera

Trace MoleculesOr

Molecular Modifications

Strong fundamental Vibrations (MIR) vs. Weak Overtone (NIR) or Raman

Lasers, Optics & Photonics, Oct 7-9, 20133

Hyperspectral Imaging

l3

l2 l1

l4

MIRIS – Mid-IR Imaging Spectroscopy Diffuse Reflection

4 InfraSignLasers, Optics & Photonics, Oct 7-9, 2013

SecurityTrace HAZMATS & Narcotics

MedicalFood Safety - Biocontamination Sensor

Pharma - Real-time in-line Quantitative QC/QA

Laser Pathology - Antibody-free Diagnosis Body FluidsTissues – Cancer Biomarkers

In-vivo diagnosis/surgery, New Microarrays

Security

Lasers, Optics & Photonics, Oct 7-9, 20135

Hand-held, battery operated, 9”x7”x4”, < 4 lbs

12”

SPoT - Sensitive Picture of Traces

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Explosive/Binder

AN

HMTD

TNT

Urea Nitrate

Sugar Urea NitrateTNT

AN

Sugar

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9.5um7.38um

R

9.25um

7.38 mm 9.5 mm

9.25 mm

3 QCLs

7

FP

DFB

DFB

SPoT raw image results of AN trace on polyethylene

9.25um7.38um 9.5um

Abso

rptio

nIn

cide

nt b

eam

s

Very thin (cloudy) film of AN on polyethylene film

9.5um

9.25um

7.38um

R

6”

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Validation

• First explosive trace imaging system sold for use by US Navy

• Customer quote: “(SPoT) continues to function well despite the traveling it has done. The hardware has proven to be remarkably robust.”

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Safety & Medical

Industrial Plant Safety/TICs Food Safety

Medical Imaging

Pharmaceutical QC/QA

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Fundamental Biomolecular Vibrations

Fatty Acids

Proteins

Sugars

Overlap

SensitivitySpecificity

PFA of 10-2 (PD of 99%)

77ug/cm2

Hyperspectral Imagingof Serum Protein

ROC Curve

PD of 99%

Anadi Mukherjee et al “Spectroscopic imaging of serum proteins using quantum cascade lasers”, Journal of Biomedical Optics 18(3), 036011 (March 2013).

Acknowledgements

Pathology Department UCSF

Professor Tarik Tihan, UCSF

Manu Prasanna

Steve Barnett

Quentin Bylund

Biswaroop Mukherjee

Block Engineering – Petros Kotidis

Adtech Optics – Mariano Troccoli

ThorLabs – John Bruno

ThanksLasers, Optics & Photonics, Oct 7-9, 201314