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Macrophage cell culture
Fig. 1: PromoCell macrophage cell culture portfolio. Overview of possible applications with PromoCell's macrophage media and cryopreserved macrophages.Abbreviations: IC = immune complexes, IFN = interferon, IgG = immunoglobulin G, GC = glucocorticoids, (G)M-CSF = (granulocyte/)macrophage colony stimulating factor, IL = interleukin, LPS = lipopolysaccharide, PBMC = peripheral blood mononuclear cells, TAM = tumor-associated macrophages (freshly isolated from tumor tissue), TGF = transforming growth factor.
Generation of macrophages
10d
or
24h
M2 macrophagesM(GC)
M(TGFβ)M(IL-10)
M(IC+LPS)M(IL-4)
M1 macrophagesM(IFN-γ)
M(LPS+IFN-γ)M(LPS)
Activation factor(s)+
Freshly isolated monocytes
Isolation of tumor-associated macrophages
Malignant (human)tumor tissue
TAM
Macrophage Base Medium DXF
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28057Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Macrophage Base Medium DXF
M2-Macrophage Generation
Medium DXFM2-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28056Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
or+ 2% human AB serum
M2 macrophagesM(-)-M-CSF
Polarized macrophagesUnpolarized macrophages
Polarized, activated macrophages (optional)
M1 macrophagesM(-)-GM-CSF
CryopreservedM1 or M2 macrophages
M0 / Mϕ macrophages
Macrophage Base Medium DXF
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28057Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Macrophage Base Medium DXF
M1- or M2-Macrophage Generation Medium DXF
M2-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28056Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M1-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28055Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M1- or M2-Macrophage Generation Medium DXF
M2-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28056Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M1-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28055Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
PromoCell provides a full range macrophage cell culture portfolio including media systems to generate macrophages from fresh peripher-al blood mononuclear cells (PBMCs) or to isolate tumor-associated macrophages (TAMs) from tumor tissues, assay-ready cryopreserved macrophages as well as subsequent macrophage analysis tools.
Macrophage cell culture Macrophage cell culture 32
In VItro Macrophage generatIon
Ready-to-use: M1/M2-Macrophage
Generation Media DXF
The PromoCell M1-Macrophage Gen-eration Medium DXF contains GM-CSF and allows for the generation of M1-polarized macrophages, whereas the M2-Macrophage Generation Medi-um DXF contains M-CSF and produces M2-polarized macrophages. Both media are ready-to-use and tested in-house for successful differentiation of M1-/M2-macrophages via flow cytometry.
User-customizable:
Macrophage Base Medium DXF
The Macrophage Base Medium DXF is the user-customizable version of the Macrophage Generation Media product line. It comes without cytokines as a universally applicable MDM culture sys-tem featuring a fully user-customizable macrophage differentiation and activa-tion process (see Fig. 3 and Tab. 1 for suggestions).
Defined and xeno-free formulation
All PromoCell Macrophage Generation Media DXF are defined and xeno-free media formulations, free of all ani-mal-derived components and substanc-es of human origin with human serum albumin as the only exception.
Efficient isolation of monocytes:
Monocyte Attachment Medium
The Monocyte Attachment Medium allows for the efficient adherence se-lection of monocytes from freshly iso-lated human mononuclear cells while maintaining optimal cell health. Thus, the time-consuming and costly immu-nomagnetic purification of monocytes is not necessary.
Product Size Catalog Number
Monocyte Attachment Medium 250 ml C-28051
Monocyte Attachment Medium
Monocyte Attachment Medium
Macrophage Generation Medium DXF
Macrophage Base Medium DXF
Product Size Catalog Number
Product Size Catalog Number
M1-Macrophage Generation Medium DXF (incl. GM-CSF) 250 ml C-28055
M2-Macrophage Generation Medium DXF (incl. M-CSF) 250 ml C-28056
Macrophage Base Medium DXF 250 ml C-28057
GM-CSF 10 µg C-60422
M-CSF 10 µg C-60420
M1/M2 Ready-to-use Macrophage Generation Media
Macrophage Base Medium DXF
Fig. 2: Non-activated human mono-cyte-derived macrophages (MDM) dif-ferentiated directly from PBMC. Mono-cytes were purified using the Monocyte Attachment Medium and differentiated in Macrophage Base Medium DXF contain-ing 100 ng/ml GM-CSF for 10 days.
Intended monocyte-derived macrophages (MDM) activation state by addition of the respective activation factor(s)*
Polarization state M2M1
IFN-γ LPS+IFN-γ LPS — — — DEX IL-10 IgG+LPS IL-4 —TGF-β1 Activation factor(s)
GM-CSF or M-CSF GM-CSF or M-CSF GM-CSF or M-CSF GM-CSF 2% hAB serum M-CSF M-CSF M-CSF M-CSF M-CSF 2% hAB serumM-CSF Differentiation factor
M(IFN-γ) M(LPS+IFN-γ) M(LPS) M(-)-GM-CSF M0 / Mϕ M(-)-M-CSF M(GC) M(IL-10) M(IC+LPS) M(IL-4) TAMM(TGFβ) MDM activation stateProduct
Macrophage Base Medium DXF (C-28057)
M1-Macrophage Generation Medium DXF incl. GM-CSF (C-28055)
M2-Macrophage Generation Medium DXF incl. M-CSF (C-28056)
Tab. 1: In vitro macrophage generation with the PromoCell macrophage media system. Overview of the wide spectrum of macrophage polarization and activation states achievable with PromoCell's macrophage generation media. Abbreviations: DEX = dexamethasone, IC = immune complexes, IFN = interferon, IgG = immunoglobulin G, GC = glucocorticoids, (G)M-CSF = (granulocyte/)macrophage colony stimulating factor, h = human, IL = interleukin, LPS = lipopolysaccharide, PBMC = peripheral blood mononuclear cells, TAM = tumor-associated macrophages (freshly isolated from tumor tissue), TGF = transforming growth factor. *Nomenclature by used activation factor(s)
In Vitro Macrophage Generation– How to choose the right medium for your macrophages –
Fresh PBMC
Unpolarized
Polarized, non-activated
Polarized, activated
Adherent monocytes
M2 macrophagesM(-)-M-CSF
M2 macrophagesM(GC)
M(TGFβ)M(IL-10)
M(IC+LPS)M(IL-4)
M2 macrophagesM(GC)
M(TGFβ)M(IL-10)
M(IC+LPS)M(IL-4)
M2 macrophagesM(-)-M-CSF
M1 macrophagesM(-)-GM-CSF
M1 macrophagesM(IFN-γ)
M(LPS+IFN-γ)M(LPS)
M1 macrophagesM(IFN-γ)
M(LPS+IFN-γ)M(LPS)
M1 macrophagesM(-)-GM-CSF
Monocyte Attachment Medium
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28051Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Monocyte Attachment Medium
Pola
riza
tion
Act
ivat
ion
(opt
iona
l)
+ M-CSF
+ G
M-C
SF
Unpolarized macrophagesM0/Mϕ
+ 2%
hum
an
AB se
rum
Customized
Macrophage Base Medium DXF
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28057Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Macrophage Base Medium DXF
M1-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28055Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M2-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28056Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M2-Macrophage Generation Medium DXF
(incl. M-CSF)
M1-Macrophage Generation Medium DXF
(incl. GM-CSF)
M(GC) M(IL-10) M(IC+LPS) M(IL-4)M(TGFβ)
Abbreviations: DEX = dexamethasone, IC = immune complexes, IFN = interferon, IgG = immunoglobulin G, GC = glucocorticoids, (G)M-CSF = (granulocyte/)macrophage colony stimulating factor, h = human, IL = interleukin, LPS = lipopolysaccharide, PBMC = peripheral blood mononuclear cells, TAM = tumor-associated macrophages (freshly isolated from tumor tissue), TGF = transforming growth factor. *Nomenclature by used activation factor(s)
+ L
PS
+ L
PS +
IFN
-γ
+ IF
N-γ
+ D
EX
+ T
GFβ
+ Ig
G +
LPS
+ IL
-10
+ IL
-4
M(GC) M(IL-10) M(IC+LPS) M(IL-4)M(TGFβ)
+ D
EX
+ T
GFβ
+ Ig
G +
LPS
+ IL
-10
+ IL
-4
M(IFN-γ) M(LPS+IFN-γ) M(LPS)
+ L
PS
+ L
PS +
IFN
-γ
+ IF
N-γ
M(IFN-γ) M(LPS+IFN-γ) M(LPS)
www.promocell.com | PromoCell GmbH | Sickingenstr. 63/65 | 69126 Heidelberg | GermanyPhone: +49 6221-649 34 0 | Fax: +49 6221-649 34 40 | info@promocell.com
More information and detailed protocols are available in the application note: Convenient large-scale differentiation of fully customized monocyte-derived macrophages from human PBMC in vitro
GM-CSF
M-CSF
—
Intended monocyte-derived macrophages (MDM) activation state by addition of the respective activation factor(s)*Cytokines
in medium
Polarization state M2M1
IFN-γ LPS+IFN-γ LPS — — — DEX IL-10 IgG+LPS IL-4 —TGF-β1 Activation factor(s)
GM-CSF or M-CSF GM-CSF or M-CSF GM-CSF or M-CSF GM-CSF 2% hAB serum M-CSF M-CSF M-CSF M-CSF M-CSF 2% hAB serumM-CSF Differentiation factor
M(IFN-γ) M(LPS+IFN-γ) M(LPS) M(-)-GM-CSF M0 / Mϕ M(-)-M-CSF M(GC) M(IL-10) M(IC+LPS) M(IL-4) TAMM(TGFβ) MDM activation stateProduct
Macrophage Base Medium DXF (C-28057)
M1-Macrophage Generation Medium DXF (C-28055)
M2-Macrophage Generation Medium DXF (C-28056)
TAM
Customized
Macrophage Base Medium DXF
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28057Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Macrophage Base Medium DXF
+ 2
% h
uman
AB
seru
m
Malignant (human) tumor tissue
In Vitro Macrophage Generation– How to choose the right medium for your macrophages –
Fresh PBMC
Unpolarized
Polarized, non-activated
Polarized, activated
Adherent monocytes
M2 macrophagesM(-)-M-CSF
M2 macrophagesM(GC)
M(TGFβ)M(IL-10)
M(IC+LPS)M(IL-4)
M2 macrophagesM(GC)
M(TGFβ)M(IL-10)
M(IC+LPS)M(IL-4)
M2 macrophagesM(-)-M-CSF
M1 macrophagesM(-)-GM-CSF
M1 macrophagesM(IFN-γ)
M(LPS+IFN-γ)M(LPS)
M1 macrophagesM(IFN-γ)
M(LPS+IFN-γ)M(LPS)
M1 macrophagesM(-)-GM-CSF
Monocyte Attachment Medium
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28051Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Monocyte Attachment Medium
Pola
riza
tion
Act
ivat
ion
(opt
iona
l)
+ M-CSF
+ G
M-C
SF
Unpolarized macrophagesM0/Mϕ
+ 2%
hum
an
AB se
rum
Customized
Macrophage Base Medium DXF
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28057Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Macrophage Base Medium DXF
M1-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28055Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M2-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28056Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M2-Macrophage Generation Medium DXF
(incl. M-CSF)
M1-Macrophage Generation Medium DXF
(incl. GM-CSF)
M(GC) M(IL-10) M(IC+LPS) M(IL-4)M(TGFβ)
Abbreviations: DEX = dexamethasone, IC = immune complexes, IFN = interferon, IgG = immunoglobulin G, GC = glucocorticoids, (G)M-CSF = (granulocyte/)macrophage colony stimulating factor, h = human, IL = interleukin, LPS = lipopolysaccharide, PBMC = peripheral blood mononuclear cells, TAM = tumor-associated macrophages (freshly isolated from tumor tissue), TGF = transforming growth factor. *Nomenclature by used activation factor(s)
+ L
PS
+ L
PS +
IFN
-γ
+ IF
N-γ
+ D
EX
+ T
GFβ
+ Ig
G +
LPS
+ IL
-10
+ IL
-4
M(GC) M(IL-10) M(IC+LPS) M(IL-4)M(TGFβ)
+ D
EX
+ T
GFβ
+ Ig
G +
LPS
+ IL
-10
+ IL
-4
M(IFN-γ) M(LPS+IFN-γ) M(LPS)
+ L
PS
+ L
PS +
IFN
-γ
+ IF
N-γ
M(IFN-γ) M(LPS+IFN-γ) M(LPS)
www.promocell.com | PromoCell GmbH | Sickingenstr. 63/65 | 69126 Heidelberg | GermanyPhone: +49 6221-649 34 0 | Fax: +49 6221-649 34 40 | info@promocell.com
More information and detailed protocols are available in the application note: Convenient large-scale differentiation of fully customized monocyte-derived macrophages from human PBMC in vitro
GM-CSF
M-CSF
—
Intended monocyte-derived macrophages (MDM) activation state by addition of the respective activation factor(s)*Cytokines
in medium
Polarization state M2M1
IFN-γ LPS+IFN-γ LPS — — — DEX IL-10 IgG+LPS IL-4 —TGF-β1 Activation factor(s)
GM-CSF or M-CSF GM-CSF or M-CSF GM-CSF or M-CSF GM-CSF 2% hAB serum M-CSF M-CSF M-CSF M-CSF M-CSF 2% hAB serumM-CSF Differentiation factor
M(IFN-γ) M(LPS+IFN-γ) M(LPS) M(-)-GM-CSF M0 / Mϕ M(-)-M-CSF M(GC) M(IL-10) M(IC+LPS) M(IL-4) TAMM(TGFβ) MDM activation stateProduct
Macrophage Base Medium DXF (C-28057)
M1-Macrophage Generation Medium DXF (C-28055)
M2-Macrophage Generation Medium DXF (C-28056)
TAM
Customized
Macrophage Base Medium DXF
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28057Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
Macrophage Base Medium DXF
+ 2
% h
uman
AB
seru
m
Malignant (human) tumor tissue
+
+ +
SupplementMix
SupplementMix
GM-CSF or
M-CSF
GM-CSF or
M-CSF
availableseparately
+
Fig. 3: In vitro macrophage generation with the PromoCell macrophage media system. Overview of the wide spectrum of macrophage polarization and activation possibilities with our media system. Abbreviations: DEX = dexamethasone, IC = immune complexes, IFN = interferon, IgG = immunoglobulin G, GC = glucocorticoids, (G)M-CSF = (granulocyte/)macrophage colony stimulating factor, h = human, IL = interleukin, LPS = lipopolysaccharide, PBMC = peripheral blood mononuclear cells, TGF = transforming growth factor.
( )
Macrophage cell culture4
assay-ready: cryopreserVed Macrophages
Macrophage Culture Made Easy:
Cryopreserved Macrophages
No time-consuming in vitro generation Ready-to-use after 24h Convenient access to individual donors Optional activation possible Long-term culture is supported with full functionality
Being part of PromoCell's unique range of macrophage culture products, cryo-preserved human macrophages are now available as a reliable source of stan-dardized cells in a ready-to-use format allowing for full experimental flexibility (Fig. 4). The frozen macrophages are produced in PromoCell´s well-proven M1/M2-Macrophage Generation Media DXF and are available as fully quali-fied M1-(hMDM-GMCSF(-)) or M2- (hMDM-MCSF(-)) polarized cells. The cells can be seeded directly into fi-bronectin-coated multiwell-plates, dishes and flasks. After plating, the mac-rophages can be maintained as biolog-ically functional, adherent cultures (see Fig. 4) for several weeks. Optionally, user-customizable activation of the cells can be performed.
Each lot is tested for cell morphology, adherence rate and viability. Further-more, they are characterized by flow cytometric analysis of relevant markers (Fig. 5): M1: CD68+, CD 80+
M2: CD68+, CD163+
Coat with Fibronectin(C-43060)
Optional: macrophage activation (24h after plating)
4h 4h
Culture of cryopreserved human macrophages
M1-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28055Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M2-Macrophage GenerationMedium DXF (Ready-to-use)
PromoCell GmbHHeidelberg, Germanywww.promocell.com
Addition by user:SupplementMix:
Date: __________
Cat. No.: C-28056Lot No.: Sample Volume 250 mlExp.: Sample Store at: + 4°C
For research use only for research use only for research use only for research use only
M2-Macrophage Generation Medium DXF
(incl. M-CSF; C-28056)
M1-Macrophage Generation Medium DXF
(incl. GM-CSF; C-28055)
Cryopreserved hMDM-GMCSF(-)(C-12914, C-12916)
Cryopreserved hMDM-MCSF(-)(C-12915, C-12917)
M2 macrophagesM(GC)
M(TGFβ)M(IL-10)
M(IC+LPS)M(IL-4)
M2 macrophages
M1 macrophagesM(IFN-γ)
M(LPS+IFN-γ)M(LPS)
M1 macrophages
M(GC) M(IL-10) M(IC+LPS) M(IL-4)M(TGFβ)
+ L
PS
+ L
PS +
IFN
-γ
+ IF
N-γ
+ D
EX
+ T
GFβ
+ Ig
G +
LPS
+ IL
-10
+ IL
-4
M(IFN-γ) M(LPS+IFN-γ) M(LPS)
Activation factor+Activation factor+
Start assay directly(24h after plating)
Start assay after activation
Fig. 4: Culture of PromoCell's cryopreserved human macrophages. The macrophages can be directly used for experiments after thawing and plating. Optional: activation of polarized M1-/M2 macrophages 24h before assay. For more information see our application notes at www.promocell.com/scientific-resources/application-notes. Abbreviations: DEX = dexamethasone, IC = immune complexes, IFN = interferon, IgG = immunoglobulin G, GC = glucocorticoids, (G)M-CSF = (granulocyte/)macrophage colony stimulating factor, h = human, IL = interleukin, LPS = lipopolysaccharide, PBMC = peripheral blood mononuclear cells, TGF = transforming growth factor.
Product Size Catalog Number
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor 1.5 Mio C-12914
Human M1 Macrophages (GM-CSF), Monocyte-derived, single donor 5 Mio C-12916
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor 1.5 Mio C-12915
Human M2 Macrophages (M-CSF), Monocyte-derived, single donor 5 Mio C-12917
Fibronectin Solution, human (1 mg/ml) 5 ml C-43060
Cryopreserved Human Macrophages
Custom solutions:
Do you require a custom produced lot or specific donor characteristics? Contact us!
custom.solutions@promocell.com
Macrophage cell culture 5
Fig. 7: Human monocyte-de-rived macrophages: transfected with a galactosidase-expressing plasmid.
Fig. 6: Macrophage phagocytosis assay. Day 10 M2-Macrophages were exposed to flourescently labeled E.coli for 24 hours before re-moving non-ingested bacteria. Left: phase contrast image. Right: fluorescence image of the phagocytizing macrophages.
Product Size Catalog Number
Product Size Catalog Number
Product Size Catalog Number
Macrophage Detachment Solution DXF 250 ml C-41330
Phagocytosis Assay Kit (Green E. coli) 100 assays PK-CA577-K963
Phagocytosis Assay Kit (Red E. coli) 100 assays PK-CA577-K964
Phagocytosis Assay Kit (Green Zymosan) 100 assays PK-CA577-K397
Phagocytosis Assay Kit (Red Zymosan) 100 assays PK-CA577-K398
PromoFectin-Macrophage 100 µl PK-CT-2000-MAC-10
PromoFectin-Macrophage 500 µl PK-CT-2000-MAC-50
Macrophage Detachment Solution
The Macrophage Detachment Solution DXF is a non-enzymatic reagent especially optimized for the gentle detachment of strongly adherent macrophages.
Phagocytosis Assay
Our Phagocytosis Assay Kits utilize pre-labeled E. coli or Zymosan particles as a tool for rapid and accurate detection and quantifi-cation of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. Our kits provide a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like Receptor (TLR) ligands.
PromoFectin-Macrophage
PromoFectin-Macrophage has been developed for optimal transfection of the hard-to-transfect macrophages and macrophage-de-rived cell lines such as RAW 264.7 by binding to mannose-specific membrane receptors. It also works with dendritic cells.
Fig. 5: Flow cytometric analysis of PromoCell cryopreserved Human M1 and M2 Macrophages. M1 macro-phages exhibit a CD68+ (99.74%) and CD80+ (83.33%) marker expression profile, typical for M1 macrophages (upper row). M2 macrophages exhibit a CD68+ (99.72%) and CD163+ (94.91%) marker expression profile, typical for M2 macrophages (lower row).
CD68 – CD68 +
positive: 99.74%
100 101 102 103 1040
20
40
60
80
100
CD80 – CD80 +
positive: 83.33%
100 101 102 103 1040
20
40
60
80
100
positive: 99.72%
CD68 – CD68 +
100 101 102 103 1040
20
40
60
80
100 positive: 94.91%
CD163 – CD163 +
100 101 102 103 1040
20
40
60
80
100
M1
M2
Assays and Reagents for Macrophage Cell Culture
PromoCell GmbH
Sickingenstr. 63/6569126 HeidelbergGermany
Email: info@promocell.comwww.promocell.com
USA/CanadaPhone: 1 – 866 – 251 – 2860 (toll free)Fax: 1 – 866 – 827 – 9219 (toll free)
DeutschlandTelefon: 0800 – 776 66 23 (gebührenfrei)Fax: 0800 – 100 83 06 (gebührenfrei)
FranceTéléphone: 0800 – 90 93 32 (ligne verte)Téléfax: 0800 – 90 27 36 (ligne verte)
United KingdomPhone: 0800 – 96 03 33 (toll free)Fax: 0800 – 169 85 54 (toll free)
Other CountriesPhone: +49 6221 – 649 34 0Fax: +49 6221 – 649 34 40
© PromoCell GmbH
Cytokines & Activation Factors Size Catalog Number
ELISAs Size Catalog Number
Dexamethasone 1 g PK-CA577-1042-1G
IFN-γ (E.coli) / IFN-γ (Plant) 100 µg* C-60724 / C-60724A
M-CSF (E.coli) / M-CSF (Plant) 10 μg* C-60442 / C-60442A
GM-CSF (E.coli) / GM-CSF (Plant) 20 µg* / 10 μg* C-60424 / C-60420A
Cryo-SFM 30 ml C-29910
IL-1 beta (E.coli) / IL-1 beta (HEK) 10 µg* / 10 μg* C-61120 / C-61121
IL-4 (E.coli) / IL-4 (Plant) / IL-4 (HEK) 20 µg* / 10 µg* / 10 µg* C-61421 / C-61420A / C-61452
IL-6 (E.coli) / IL-6 (CHO) / IL-6 (HEK) 20 µg* / 10 µg* / 10 µg* C-61625 / C-61640 / C-61642
IL-10 (E.coli) / IL-10 (HEK) 10 µg* C-62012 / C-62014
IL-12 (CHO) / IL-12 (HEK) 10 µg* C-62213 / C-62212
IL-13 (E.coli) 10 µg* C-62312
MCP-1 (CCL2) 10 µg* C-64030
MIP-1 alpha (CCL3) / MIP-3 alpha (CCL20) 20 µg* / 20 µg* C-65833 / C-65866
TGF-β1 (E.coli) / TGF-β1 (CHO) / TGF-β1 (HEK) 10 µg* C-63500 / C-63503 / C-63499
*bulk sizes (100/250/500 and 1000 µg) are available on requestMore cytokines/chemokines/growth factors are available at www.promocell.com/cytokines-growth-factors.
TNF-α ELISA Kit, human 96 tests PK-EL-63709
TGF-β1 ELISA Kit, human 96 tests PK-EL-63506
TGF-β2 ELISA Kit, human 96 tests PK-EL-63508
IL-1α ELISA Kit, human 96 tests PK-EL-61106
IL-1 beta ELISA Kit, human 96 tests PK-EL-61128
IL-6 ELISA Kit, human 96 tests PK-EL-61606
IL-8 ELISA Kit, human 96 tests PK-EL-61806
IL-10 ELISA Kit, human 96 tests PK-EL-62006
IL-12 (p40) ELISA Kit, human 96 tests PK-EL-62215
IL-12 (p40+p70) ELISA Kit, human 96 tests PK-EL-62207
IL-12 (p70) ELISA Kit, human 96 tests PK-EL-62216
IL-23 ELISA Kit, human 96 tests PK-EL-63006
IL-27 ELISA Kit, human 96 tests PK-EL-62930
IP-10 (CXCL10) ELISA Kit, human 96 tests PK-EL-65521
More ELISAs are available at www.promocell.com/elisas.
Related Products
Cell-based Assays Size Catalog Number
Cell Migration/Chemotaxis Assay Kit (8 µm, 24-well) 12 Assays PK-CA577-K909
Cell Migration/Chemotaxis Assay Kit (8 µm, 96-well) 96 Assays PK-CA577-K906
Cell Migration/Chemotaxis Assay Kit (5 µm, 24-well) 12 Assays PK-CA577-K910
Cell Migration/Chemotaxis Assay Kit (5 µm, 96-well) 96 Assays PK-CA577-K907
Cell Migration/Chemotaxis Assay Kit (3 µm, 24-well) 12 Assays PK-CA577-K911
Cell Migration/Chemotaxis Assay Kit (3 µm, 96-well) 96 Assays PK-CA577-K908
More products and information are available at www.promocell.com/cell-model-systems.
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