legame cometitivo al dna mascheramento della superficie di attivazione interazione con fattori...

Legame cometitivo al DNA

Mascheramento della superficie di attivazione

Interazione con Fattori generali della trascrizione

Reclutamento di complessi di rimodellamento della cromatina repressivi

Reclutamento di istone deacetilasi

HELICASE/ATPase domain

BROMO domainbinds acetylated lysines on histone tails

HSA domain protein interactions

actin/ARP transcription factors

ATPase domain

ROLE of SNF2/BRM domains

SNF2 ATPase activitychange nucleosome positionincreased regulatory protein access!change nucleosome conformationeject histone octamer

displace H2A/H2B dimer

Roles of SWI2/SNF2Role in activation or repression of genesInducible gene expression:transcription initiationtranscription elongation

SplicingRepair

Roles of ISWInucleosome array formationchromatin assembly, replication

heterochromatin formationtranscriptional regulation some PolII, PolI

SANT/HAND domain contacts histone tails

Slide domain linker DNA contact, ’measures’ distanceequal spacing of nucleosomes

ATPase domain

ROLE of ISWI domains

CHD ATPasesCHD1: role in chromatin assembly; open chromatinin pluripotent cellsNuRD complex also contains Me-DNA binding protein (MBD2)complex connects deacetylation, chromatin remodeling and DNA methylation; repressive function

CHROMO and PHD domainsbind methylated lysines on histone tailsmodulate activity of remodelers

ATPase domain

ROLE of CHD ATPase domains

INO80chromatin assemblyDNA repairinteracts with phosphorylated H2A.X (gammaH2A.X)transcription

SWR1H2A exchange with H2A.ZBoundary to heterochromatin spreadingtranscriptionally poised promoters (together with H3.3)

WINAC-WICHWSTF Williams syndrome transcription factor

helix–turn–helix motif (HTH)

tight chromatin binding

stepwise assembly model for transcription initiation.