identification & cultivation
Post on 25-May-2015
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ISOLATION AND CULTIVATION OF VIRUSES
Cultivation and identification of viruses
What are viruses?
Viruses are infectious ,intracellular,obligate,parasites
Methods to cultivate animal viruses:-
1) Embryonated Chick egg
2)Live animals
3)Tissue culture
The primary purposes of viral cultivation are:
1. to isolate and identify viruses in clinical specimens
2. to prepare viruses for vaccines
3. to do detailed research on viral structure, multiplication cycles, genetics, and effects on host cells.
1.Good Pasteur2.Burnel-Modified3.Animal eggs eg:-CHICKEN,DUCK and TURKEY
4.5to12 days old eggs
Tissues Viruses(cultivated)
Choriallantonicmembrane
Herpes,Small pox virus,Rous Sarcoma virus
Amniotic cavity Mumps virus ,Influenza
Yolk Sac Yellow fever,Rabies
Embryo Influenza ,Mumps
Sites suitable for cultivation of viruses :
Viral culture in eggs: Some viruses, such as influenza viruses, are grown in embryonated chicken eggs
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