hematology analyzer using the idexx vetautoread ...hematology analyzer and close the lid. wait for...

n Running a Test

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1-800-248-2483

n Whole-Blood Sample Collection

Using the IDEXX VetAutoread™ Hematology Analyzer

Sample Selection and Preparation The IDEXX VetAutoread™ analyzer is designed to analyze whole-blood samples. Good sample collection and preparation are necessary for optimum results on the IDEXX VetAutoread™ Hematology Analyzer.

Please refer to your IDEXX VetAutoread™ Hematology Analyzer Operator’s Guide for complete instructions.

IDEXX VetAutoread™ Hematology Analyzer

One IDEXX Drive, Westbrook, Maine 04092 USA idexx.com

© 2007 IDEXX Laboratories, Inc. All rights reserved. • 09-64985-01 Practice what's possible, VetTube and VetCentrifuge are trademarks or registered

trademarks of IDEXX Laboratories, Inc. in the United States and/or other countries.VetAutoread is a trademark of QBC Diagnostic, Inc.

n Tips for Best Practices

• Analyzethesamplewithinfourhoursofcollection.• Discardseverelyhemolyzed,lipemicorclottedsamples.• Donotuseexpiredtubes.• Uselint-freewipestoremoveanybloodorfingerprintsfromtheoutsideofthetube.• Runthecalibrationroddaily.

A. B.

NOTE: It is recommended that automated hematology differentials be complemented with a blood film for proper identification of abnormal cells and morphology.

Afterthepatientinformationisentered,placethetubeintheIDEXXVetAutoread™HematologyAnalyzerandclosethelid.Waitfortheresults.

lock pipettoropen pipettor

n Preparation of an IDEXX VetTube™

3.Depressandholddowntheplunger.Inserttheacridineorange-coatedendofthetubeintothemiddleofthesample.Releasetheplungersmoothly.Ensurethatthebloodreachesthe111-µLblackfillline.

4.Liftupthepipettor.Wipetheoutsideofthetubesurfacewithalint-freewipe.Insertthetubeendintothecapinthetray.Securebytwistingthepipettorahalf-turn.

5.Holdthepipettorhorizontallyandunlockthebarrelbytwistingitclockwise.Gentlyremovethetube.

7a. Insertthefloat.Holdthetubehorizontallyandslideitoverthetipofthefloat.Holdthetubeclosetothegreenlines,liftthetubeanddislodgethefloatfromtheslot.Touchthetubeendagainstthebackofthetray.NEVERTOUCHTHEFLOATWITHYOURFINGERS.

7b. Alternatemethod:Useforcepstoinsertthefloat.

8.CentrifugethetubeintheIDEXXVetCentrifuge™for5minutes.Makesuretobalancethecentrifugewithanothertubedirectlyoppositethesample.Ensurethatthecentrifugecoverisproperlyfastened.(Bovinesamplesshouldbespuntwicewithathree-minutewaitinbetweentoensurecoolingofthecentrifuge.)

9.Promptlyremovethetube.Wipethetubetomakesurethatitiscleanfrombloodorfingerprints.Ensurethattheplasmalevelisbetweenthetwogreenlines.Ifnot,discardthetubeandprepareafreshtube.

2.Usetheappropriatesample-collectiondevices.

1.Usetheappropriatetube(EDTAforcanine,felineorequineblood,andsodiumcitrateforbovineblood).

3.Dispensethesampleintothepropertubeandfilltothemanufacturer’sspecifications(generally½to¾full).Donotdepresstheplungerforcefully.Ifusingasyringe,removetheneedle.

4.Gentlyinvertthetubefor30seconds(atleast10times)tomix.

1. Withthenozzlepointingawayfromyou,unlockthepipettorbytwistingthebarrelclockwise.Insertthetubeendwithgreenlinesintothebarrel.Lockitinplacebytwistingthebarrelcounterclockwise.

2.IfthesamplesitsbetweentransferintotheEDTAtubeanddrawingintotheIDEXXVetTube,gentlyinvertthetubefor30seconds(atleast10times)tomix.Forbovinesamples,refertothebovine-specificsamplepreparationkit.

6.Holdthetubehorizontallyandrollitbetweenyourfingersforatleast30secondstoensureadequatestaining.