genetic engineering of plant: agrobacterium · 2010. 8. 20. · left 24b re eat virulence region...
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Department of BiologyBogor Agricultural University
2010
Department of BiologyBogor Agricultural University
2010
Genetic Engineering of Plant:Agrobacterium
Genetic Engineering of Plant:Agrobacterium
Aris Tri Wahyudi, PhD
Soil Gram Negative Bacterium
T-DNA dan Opines
Agrobacterium tumefaciens as a toolfor genetic engineering
Problem : Tumor
How can we engineer the Ti plasmid to make Useful???Delete auxin and cytokinin genesRetain vir Genes; LB and RB, OriTi plasmid is ~ 120 kb, need to make it smaller
Biochemistry of plant tumors
Agrobacterium induced-plant tumors contain
High concentration of:
Plant hormone : auxin, cytokinin
Opine : Octopine, nopaline,
Agrocinopine, mannopine
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