eco™48 fast facts · 2016-09-07 · fast facts specs confidential 3 •48 well in a 384 well...

Eco™48 Fast Facts

2014

Intro

2

• Fast Fact about the ECO Spec

Thermal system

Optical system

Competitive overview

Common objections

Fast Facts Specs

3 CONFIDENTIAL

• 48 well in a 384 well format (5-20ul rxn volumes).

• +/-0.1°C, typically x5 better than standard block based system.

• 4 colour multiplex.

• 40 cycles in 40 mins, ramp rate of 5.5°C/second (up and down).

• Eco Study to allow pooling of multiple plate runs and analyse as one experiment.

• Open platform, supports all chemistries and has free software, no licences.

• Adaptive LED Control (ALC) for superior sensitivity, speed and data quality (you will never saturate the CCD chip or have bleed though between wells, no matter how strong the signal).

Thermal system – The Block

• Hollow fluid filled silver block anodised with gold.

• Unparalleled uniformity (+/- 0.1 degrees at 95degrees).

• Brushless drive motors.

• XLL (extra long life) single peltier unit, which gives the uniformity and the 384 well format of the block, combined with the 5.5degrees/sec ramp rate make the Eco™48 very fast.

Optical system

Key components

Two banks of 48 LEDs (Blue/Green)

CCD camera

Four position filter magazine

Examples of Eco-Compatible Dyes

Channel Dye

Channel 1 (λ = 505-545 nm) SYBR Greena, FAMa

Channel 2 (λ = 604-644 nm) ROXa, Texas Red

Channel 3 (λ = 562-596 nm) HEXa, JOE, TET, VICa

Channel 4 (λ = 665-705 nm) Cy5a, Q670a

Adaptive LED Control (ALC)

ALC is a proprietary firmware algorithm that normalises variation in fluorescence across all 48 wells of the Eco™ plate at each cycle of a run.

Minimizes %CV across the plate

to prevent well-to-well cross talk

Provides specific tuning for each

LED following each PCR cycle

Maximizes the linear range of

detection

ALC is a unique feature to Eco™48 which maximises

the performance of the system

• By normalising fluorescence emission across all 48

wells of the plate at each cycle ALC ensures:

- Minimimal Cross Talk from well to well, to improve

specificity of a results

- Minimimal %CV, to improve accuracy of data

- Increase Sensitivity by adjusting exposure for low

detection level

- Maximizes the linear range of emission detection to get

the most out of your samples

• Eco™48’s ALC and Extreme Thermal Uniformity are paired to generate reliable data

Benefits

Competition analysis

System Features

PCRmax

Eco™48

Roche Lightcycler

480

Roche Lightcycler

2.0

Roche Lightcycler

Nano

ABI

7500 FAST

ABI StepOne & StepOne+

ABI Quant

studio 6,7,12

Uniformity ±0.1˚C +0.4˚C +0.2˚C +0.1˚C ±0.5˚C ±0.5˚C ?

Standard Run time

40 min <90 min <50 min <50 min <120 min <120 min ? min

Multiplexing 4 colours 6 colours 6 colours 2 colours 5 colours 3 colours 5/6 colours

# of wells 48 96/384 32 32 96 48/96 96/384

HRM Included Included Included Included Included Not Available Included

Speed 5.5˚C/sec 4.4˚C/sec 20˚C/sec 5.0˚C/sec 5.5˚C/sec 4.6˚C/sec ?

Price List $19.8K List $40K

Promo $32K List $40K

List $22K

Promo $15K List $38K

List $32K / $36K

Promo $23K / $26K ?

Competition analysis

System Features

PCRmax

Eco™48

Bio-Rad

CFX96 Touch /

CFX384 Touch

Bio-Rad

Mini Opticon

Qiagen Rotor-Gene Q

Bioneer

Exicycler 96

Analytik-jena

qTower

DNA Technologies

- Dt Lite

Uniformity ±0.1°C ±0.4˚C ±0.4˚C ±0.02˚C ±0.4˚C ±0.2˚C ±0.2˚C

Standard Run time

40 min <90 min <120 min 45 min <120mins <20mins <120min

Multiplexing 4 colours 5 colours 2 colours 6 colours 5 colour 4 colour 4-5 colour

# of wells 48 96/384 48 100 96 96 48

HRM Included Included Not Available Included Not

Available Not

Available Not Available

Speed 5.5˚C/sec 5.0˚C/sec 2.5˚C/sec 15˚C/sec 2.5˚C/sec 12˚C/sec 5.0˚C/sec

Price List $19.8K List $31K

Promo $24K List $19K List $24K List $20k List $18k List $20k

Common objection #1

• “It’s only 48 well”

Highlight Thermal Uniformity

The Eco™48 has the best temperature uniformity of any blocked based system (+/-0.1degrees) requiring you to run fewer replicates.

The Eco™ is capable of running 40 cycles in 40 minutes.

The combination of duplicates and faster run times gives the Eco™ a fast run rate (samples/hour) compared to a standard 96 well system.

Common objections #1

All because of uniformity and speed.

• On a 96-well instrument WITHOUT good thermal uniformity

5 point std curve and NTC in triplicates = 18 wells

78 wells left = 26 samples with triplicates

• 48 well Eco™ WITH EXCELLENT thermal uniformity

5 point std curve and NTC in DUPLICATES = 12 wells

36 wells left = 18 samples in duplicates

• Define that plate as mother plate in Study software then: No need to run the Std curve again

Two plates = 24 more assays total!

Using same total 96 wells , you can actually interrogate a total of 42 samples on Eco™ compared to 26 on a 96-well instrument.

Cont’: Two Eco™48 Plates vs. a 96-well plate instrument

Common objection #1

• Demonstrate the software

Eco™48 Study

Data from multiple plates combined into one experiment

Effectively increase throughput, see above.

Combine multiple instrument results.

Benefits

Higher throughput.

Almost limitless sample size.

Common objection #2

• Price!!

Competition are dropping their price.

Competition are bundling chemistries

It’s more expensive than X,Y,Z

Common objection #2

Push the features of the Eco™48

Common objection #2

• Price is always there, but it’s the running etc that costs.

• Sample, reagent, time costs. Can easily run 5ul in an Eco™48, typically 20-50ul in a 96 well.

• Accuracy and specificity is what will count in the long run.

• We supply the chemistries, so all good.

“Quality is remembered long after the price is forgotten” Henry Royce

Common Objection #3

• “Yeah, but its still only 48 wells”

If somebody is not swayed by the Study software, the explanation of

the uniformity, the cost saving, the time saved and the features and still is fixated on 96 well format.

» Watch this space………….

Why the Eco™48

• 5X better thermal performance (uniformity)

• Industry-leading data quality

• Features to price heavy

• System includes loading dock

Key

Features

System

• 48-well format

• 4 colour multiplex

• Streamlined MIQE compliant software for

novices and experts

• Compact footprint

Key

Applications

• Abs/Relative quantification, Allelic

discrimination, viral load, HRM

• Standard and FAST cycling protocols

• Supports all Real-Time PCR chemistries

Optical system – calibrated dyes

Examples of Eco-Compatible Dyes

Channel Dye

Channel 1 (λ = 505-545 nm) SYBR Greena, FAMa

Channel 2 (λ = 604-644 nm) ROXa, Texas Red

Channel 3 (λ = 562-596 nm) HEXa, JOE, TET, VICa

Channel 4 (λ = 665-705 nm) Cy5a, Q670a

• Each Eco™48 comes factory calibrated for the most typical dyes.

• Two types of software

Eco Control

Eco Study

• All software is

Free of licence

Controlled by PCRmax

Fully capable (no bolt on applications)

Eco Control

• Eco Control is used to drive the system

Define

Application (HRM, Genotyping, Quantification)

Chemistries (DBD, Hydrolysis Probes)

Starting materials

Quantification types (Abs/Rel)

Thermal profile and cycles

Plate layout

Eco Control does no analysis at all, only monitoring of run and set up.

Home screen

Thermal Profile

Plate Layout

Note: you can import plate layouts from Excel/CSV formatted

documents.

Monitor run

Applications with Eco™48

• Absolute and Relative quantification Gene Expression

NGS Library quantification

Copy Number Variation

Inserts, deletion

RNA characterisation

siRNA, miRNA

Viral load detection

• Genotyping and High Resolution Melt (HRM) Allelic Discrimination

DNA Methylation

SNP Definition

Pathogen detection

Actual Eco™48 Data

Introduction

30

• Alpha cyclers background

• The Alpha cycler range

• The software

• The specs

Alpha cycler

• Will be one of the forefront end point PCR systems in terms of spec, appearance and certainly one of the best interfaces.

• System was defined to be

Slick

Easy

High end feel, look and operation.

Alpha Cycler Range

• Consistencies across the range

Active Sample cooling

Programme Wizard

Android interface

Gradient

96/384 well formats available

USB ports

Reporting

Software, look and feel.

Alpha Cycler

Single block (96 or 384) system called AC -1

Perfect for individual groups

Flexibility of format

High features to cost

Perfect for standard though put.

Four block (96/384/combo) system called the AC-4

Four independent blocks.

High throughput system

Multiple users, not necessarily HTP.

Only true multi-block unit on the market.

The AC-1

Single block (96 or 384)

7” 1080p HD Android tablet

screen

Compact footprint

Quiet

Perfect for standard though put.

The AC-4

Four block (96/384/2+2)

10” 1080p HD Android tablet

Four fully independent blocks.

High throughput system.

Only true multi-block unit on the market.

Cost effective

Small footprint

Positioning

The Software

• Developed in Android.

• Will have familiar responses/actions as Android.

• Customisable and powerful.

The Software

The Software

The Software

The Software

The Software

The Software

The Software

The Software

The Software

The Software

The Software

The Specs

• Versus typical conventional cycler

Better interface

Report useful data potentially fault codes

More compact

High thermal performance (ramp and uniformity)

Familiarity with whole range

Alpha Cycler

• Slick

• Easy

• High end

“Ease of use backed up with superior performance”