dothiora corymbiae · 2019. 2. 8. · fungal planet description sheets 271 © 2018 naturalis...

270 Persoonia–Volume41,2018

Dothiora corymbiae

271Fungal Planet description sheets

© 2018 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute

FungalPlanet795–13December2018

Dothiora corymbiae Crous, sp. nov. Etymology.NamereferstoCorymbia, the host genus from which this funguswasisolated.

Classification—Dothideaceae, Dothideales, Dothideomycetes.

Conidiomata pycnidial, separate, obovoid, papillate, brown, with centralostiole,100–150µmdiam;wallof4–8layersofbrowntextura angularis. Conidiophores reduced to conidiogenous cells lining the inner cavity, ellipsoid, hyaline, polyphialidic, with1–3lociperconidiogenouscell,5–10×4–5µm.Conidia solitary, hyaline, smooth, guttulate, subcylindrical with obtuse ends,straight,4–5×2µm;oncedischargedonto theagarsurface, conidia turn brown, and become swollen, thick-walled, ellipsoid,5–6×3–4µm. Culturecharacteristics—Coloniesslimy,yeast-like,spread-ing, with sparse aerial mycelium and feathery, lobate margin, reaching50mmdiamafter2wkat25°C.OnMEAsurfacesepia,reversehazel.OnPDAsurfaceandreversesepia.OnOAsurfacehazeltofawn.

Typus.auStraLia,Queensland,Minden,onleavesofCorymbia citriodora (Myrtaceae),1Feb.2010,J. Roux,CJA010(holotypeCBSH-23760,isotypeBRIP58843a, cultureex-typeCPC33719=CBS145060, ITSandLSUsequencesGenBankMK047431.1andMK047482.1,MycoBankMB828175).

Notes—DothiorawasrecentlytreatedbyCrous&Groene-wald (2016,2017).Ascostromata were shown to lack pseu-doparaphyses, have bitunicate asci with hyaline, transversely septateascospores(rarelywithverticalsepta).Conidiomataare pycnidial, conidiophores reduced to hyaline, phialidic coni- diogenous cells, and conidia aseptate, hyaline, smooth, sub- cylindrical to ovoid or oblong, forming a hormonema-like synasexualmorph.Thepresentcollectionisthusbestaccom-modated in Dothiora.BasedonamegablastsearchofNCBIsGenBanknucleotide database, the closest hits using the ITSsequencehadhigh-est similarity to Neocylindroseptoria pistaciae (GenBankKF251152.1; Identities=512/577 (89%), 37 gaps (6%)),Do thiora sorbi (GenBankMH860401.1; Identities=509/590(86%), 42 gaps (7%)) andKabatina thujae (GenBankMH858858.1; Identities=484/555 (87%), 38 gaps (6%)).Closest hits using the LSUsequenceareDothiora ceratoniae (GenBankMH872233.1;Identities=818/839(97%),3gaps(0%)),Dothiora schizospora(GenBankMH868980.1;Identities=817/839(97%),2gaps(0%))andDothiora cactacearum (GenBankKY929177.1; Identities=817/839 (97%), 2gaps(0%)).

DothioraFr.,SummaVeg.Scand.,SectioPost.(Stockholm):418.1849

Synonyms.CylindroseptoriaThambug.&K.D.Hyde,Fung.Diversity68:125.2014.

NeophaeocryptopusWanas.etal.,Fung.Diversity78:21.2016.

ForadditionalsynonymsseeCrous&Groenewald(2017).

Dothiora pistaciae(Quaedvliegetal.)Crous,comb. nov.

MycoBankMB828176.

Basionym.Cylindroseptoria pistaciaeQuaedvliegetal.,Stud.Mycol. 75:359.2013.

Synonym.Neocylindroseptoria pistaciae(Quaedvliegetal.)Thambug.etal.,Fung.Diversity68:125.2014.

Dothiora mahoniae(A.W.Ramaley)Crous,comb. nov.

MycoBankMB828177.

Basionym.Kabatina mahoniaeA.W.Ramaley,Mycotaxon43:443.1992.

Dothiora cytisi(Wanas.etal.)Crous,comb. nov.

MycoBankMB828178.

Basionym.Neophaeocryptopus cytisiWanas. et al., Fung.Diversity 78:22.2016.

Colour illustrations.Indigenousforestin Queensland,Australia;colonysporulatingonmaltextractagar,conidioma,hyphae,conidiogenouscellsandconidia.Scalebars:conidioma=150µm,allothers=10µm.

PedroW.Crous&JohannesZ.Groenewald,WesterdijkFungalBiodiversityInstitute,P.O.Box85167,3508ADUtrecht,TheNetherlands;e-mail:p.crous@westerdijkinstitute.nl&e.groenewald@westerdijkinstitute.nl

JolandaRoux,ForestryandAgriculturalBiotechnologyInstitute(FABI),UniversityofPretoria,Pretoria0002,SouthAfrica;e-mail:jolanda.roux@up.ac.za