digest 2 of prevtre: 6487 bases (circular)page 1 sep 26, 2000 digest 2 of prevtre: 6487 bases...
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1Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
AatII 3 812 4728 1546-6273GACGT|C 1546 1025 6274-811
6274 734 812-1545
AccI 2 3316 5572 4231-3315GT|MKAC 4231 915 3316-4230
AciI 7 1 150 467 2805-3271C|CGC 272 392 3274-3665G|CGG 401 362 5053-5414
755 354 401-754794 302 3666-3967927 233 2458-2690968 191 5506-5696
1109 177 1930-21061200 170 6467-1491266 167 6240-64061365 164 3968-41311384 141 968-11081422 135 4909-50431538 133 794-9261662 129 272-4001727 129 2319-24471794 127 4532-46581844 124 1538-16611864 122 150-2711888 121 5743-58631910 121 4678-47981930 116 1422-15372107 113 2155-22672145 110 4799-49082155 109 5985-60932268 9 9 6094-61922296 9 9 1266-13642298 9 7 2691-27872319 9 1 5415-55052448 9 1 1109-11992458 9 1 4413-45032691 7 7 5908-59842788 6 7 1727-17932805 6 6 1200-12653272 6 5 1662-17263274 5 8 6407-64643666 5 0 1794-18433968 4 7 6193-62394132 4 6 5697-57424171 4 4 5864-59074181 4 3 4342-43844223 4 2 4181-42224248 4 1 927-9674286 3 9 755-7934299 3 9 4132-41704325 3 8 2107-2144
Sites FragmentsNumber Position Length Ends
4342 3 8 4248-42854385 3 8 1384-14214392 2 8 4504-45314413 2 8 2268-22954504 2 6 4299-43244532 2 5 4223-42474659 2 4 1864-18874678 2 2 1888-19094799 2 1 4392-44124909 2 1 2298-23185044 2 0 1844-18635053 2 0 1910-19295415 1 9 4659-46775506 1 9 1365-13835697 1 7 2788-28045743 1 7 4325-43415864 1 3 4286-42985908 1 0 2448-24575985 1 0 2145-21546094 1 0 4171-41806193 9 5044-50526240 7 4385-43916407 2 6465-64666465 2 3272-32736467 2 2296-2297
Afl II 3 3 5 3097 3425-34C|TTAAG 1073 2352 1073-3424
3425 1038 35-1072
Afl III 1 4459 6487 4459-4458A|CRYGT
AhdI 4 1087 2828 1087-3914GACNNN|NNGTC 3915 2222 5352-1086
3961 1391 3961-53515352 4 6 3915-3960
AluI 3 8 3 0 813 5704-29AG|CT 3 4 744 743-1486
120 521 5020-5540191 459 2674-3132412 385 1673-2057655 281 4120-4400735 257 4763-5019743 243 3805-4047
1487 243 412-6541599 226 4401-46261631 225 2058-22821673 221 191-4112058 219 3586-38042283 190 2377-25662348 112 1487-1598
2Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
2377 107 2567-26732567 104 3186-32892674 100 5541-56403133 9 0 4627-47163186 8 7 3424-35103290 8 6 34-1193335 8 5 3335-34193420 8 0 655-7343424 7 5 3511-35853511 7 1 120-1903586 6 5 2283-23473805 6 3 5641-57034048 5 3 3133-31854101 5 3 4048-41004120 4 6 4717-47624401 4 5 3290-33344627 4 2 1631-16724717 3 2 1599-16304763 2 9 2348-23765020 1 9 4101-41195541 8 735-7425641 4 30 -335704 4 3420-3423
Alw26 I 2 0 179 1622 1587-3208GTCTCN| 531 1311 4102-5412|NNNNNGAGAC 552 776 5413-6188
606 547 3376-3922800 477 6189-178898 352 179-530
1017 194 606-7991139 191 1396-15861198 180 1216-13951216 167 3209-33751396 122 1017-11381587 119 898-10163209 103 3999-41013376 9 8 800-8973923 5 9 1139-11973944 5 5 3944-39983999 5 4 552-6054102 2 1 531-5515413 2 1 3923-39436189 1 8 1198-1215
AlwI 1 9 1119 1617 5989-1118GGATCNNNN| 1423 1014 4006-5019|NNNNNGATCC 1511 695 3311-4005
1978 478 2778-32552298 467 1511-19772405 463 5205-56672778 373 2405-27773256 320 1978-2297
Sites FragmentsNumber Position Length Ends
3298 317 5668-59843311 304 1119-14224006 107 2298-24045020 9 6 5108-52035106 8 8 1423-15105108 8 6 5020-51055204 4 2 3256-32975205 1 3 3298-33105668 4 5985-59885985 2 5106-51075989 1 5204-5204
AlwNI 4 232 3319 232-3550CAGNNN|CTG 3551 1844 4875-231
3626 1249 3626-48744875 7 5 3551-3625
ApaLI 5 1799 2267 6019-1798G|TGCAC 2101 2174 2101-4274
4275 1246 4773-60184773 498 4275-47726019 302 1799-2100
ApoI 6 939 3063 3282-6344R|AATTY 1471 1520 1762-3281
1762 1025 6401-9383282 532 939-14706345 291 1471-17616401 5 6 6345-6400
AscI 1 444 6487 444-443GG|CGCGCC
AseI 1 5524 6487 5524-5523AT|TAAT
Asp718 I 4 477 3094 3870-476G|GTACC 3137 2660 477-3136
3294 576 3294-38693870 157 3137-3293
AvaI 9 242 2863 3866-241C|YCGRG 473 2295 473-2767
2768 502 3298-37992834 307 2834-31403141 231 242-4723298 157 3141-32973800 6 6 2768-28333833 3 3 3800-38323866 3 3 3833-3865
AvaII 1 6 261 1470 4020-5489G|GWCC 274 1036 5712-260
3Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
329 641 1286-1926627 541 1927-2467757 529 757-1285
1286 521 2736-32561927 398 3257-36542468 298 329-6262736 297 3723-40193257 268 2468-27353655 222 5490-57113668 130 627-7563723 5 5 274-3284020 5 5 3668-37225490 1 3 261-2735712 1 3 3655-3667
BamHI 1 3303 6487 3303-3302G|GATCC
BanI 1 1 319 1506 5300-318G|GYRCC 477 1430 3870-5299
1209 1044 2093-31361464 732 477-12082093 629 1464-20923137 419 3294-37123294 255 1209-14633713 158 319-4763838 157 3137-32933870 125 3713-38375300 3 2 3838-3869
BanII 9 414 2910 3991-413GRGCY|C 427 2537 598-3134
598 515 3292-38063135 171 3820-39903188 171 427-5973292 104 3188-32913807 5 3 3135-31873820 1 3 414-4263991 1 3 3807-3819
BbeI 2 1468 4113 3842-1467GGCGC|C 3842 2374 1468-3841
BbsI 3 445 3076 3254-6329GAAGACNN| 3254 2809 445-3253|NNNNNNGTCTTC 6330 602 6330-444
BbvI 2 2 970 1676 5781-969GCAGCNNNNNNNN| 978 1653 2386-4038|NNNNNNNNNNNNGCT...998 602 1006-1607
1006 419 4369-47871608 387 1872-22581660 303 5087-5389
Sites FragmentsNumber Position Length Ends
1866 206 1660-18651872 206 4881-50862259 202 5579-57802386 189 5390-55784039 144 4207-43504110 127 2259-23854207 9 7 4110-42064351 9 0 4788-48774369 7 1 4039-41094788 5 2 1608-16594878 2 0 978-9974881 1 8 4351-43685087 8 970-9775390 8 998-10055579 6 1866-18715781 3 4878-4880
BcgI 4 535 3119 2738-5856GCANNNNNNTCGNNNN...2670 2135 535-2669|NNNNNNNNNNCGANN...2738 1165 5857-534
5857 6 8 2670-2737
BcgI 4 501 3119 2772-5890CGANNNNNNTGCNNNN...2636 2135 501-2635|NNNNNNNNNNGCANN...2772 1097 5891-500
5891 136 2636-2771
BfaI 1 3 3 1 1266 3688-4953C|TAG 294 1146 1461-2606
690 976 5542-30728 721 740-1460740 703 2607-3309
1461 396 294-6892607 335 5207-55413310 267 3421-36873421 263 31-2933688 253 4954-52064954 111 3310-34205207 3 8 690-7275542 1 2 728-739
Bgl I 2 3274 4289 5472-3273GCCNNNN|NGGC 5472 2198 3274-5471
Bgl II 1 2830 6487 2830-2829A|GATCT
BlpI 1 2670 6487 2670-2669GC|TNAGC
BpmI 5 2187 3252 5422-2186CTGGAGNNNNNNNNNN...2241 2064 3358-5421|NNNNNNNNNNNNNNC...3233 992 2241-3232
4Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
3358 125 3233-33575422 5 4 2187-2240
BsaAI 1 4213 6487 4213-4212YAC|GTR
BsaHI 8 809 2050 3839-5888GR|CGYC 1465 1025 6271-808
1543 968 1543-25102511 707 2511-32173218 656 809-14643839 621 3218-38385889 382 5889-62706271 7 8 1465-1542
BsaI 8 531 2707 1216-3922GGTCTCN| 552 1605 5413-530|NNNNNGAGACC 606 1414 3999-5412
1216 610 606-12153923 5 5 3944-39983944 5 4 552-6053999 2 1 531-5515413 2 1 3923-3943
BsaJI 3 9 160 1846 4619-6464C|CNNGG 236 603 4016-4618
315 538 1333-1870325 420 814-1233473 400 2741-3140537 256 3299-3554622 231 2296-2526623 213 2527-2739761 201 1871-2071800 182 6465-159801 155 2072-2226813 148 325-472814 147 3719-3865
1234 138 623-7601303 131 3141-32711312 8 6 3929-40141332 8 5 537-6211333 7 9 236-3141871 7 9 3630-37082072 7 6 160-2352227 7 5 3555-36292296 6 9 1234-13022527 6 9 2227-22952740 6 4 473-5362741 6 3 3866-39283141 3 9 761-7993272 2 6 3272-32973298 2 0 1312-13313299 1 2 801-812
Sites FragmentsNumber Position Length Ends
3555 1 0 3709-37183630 1 0 315-3243709 9 1303-13113719 1 622-6223866 1 4015-40153929 1 2740-27404015 1 3298-32984016 1 813-8134619 1 800-8006465 1 1332-1332
BsaWI 8 1519 2252 2413-4664W|CCGGW 1740 1554 6452-1518
2277 831 4812-56422413 809 5643-64514665 537 1740-22764812 221 1519-17395643 147 4665-48116452 136 2277-2412
Bsc4 I 5 6 1 8 1562 4943-17CCNNNN|NNNGG 2 1 587 2793-3379
166 374 2419-2792167 371 4109-4479241 353 852-1204242 287 1802-2088243 279 1460-1738270 279 4664-4942439 221 2198-2418521 202 646-847542 169 270-438574 168 3664-3831575 166 4498-4663607 155 3406-3560622 145 21-165623 127 1333-1459642 109 2089-2197643 8 2 439-520646 8 0 3833-3912848 7 7 1205-1281852 7 4 167-240
1205 7 4 3562-36351282 7 0 4039-41081332 6 3 1739-18011333 5 0 1282-13311460 3 3 3967-39991739 3 2 3934-39651802 3 2 542-5732089 3 2 575-6062198 2 7 3637-36632419 2 7 243-2692793 2 6 3380-34053380 2 1 3913-3933
5Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
3406 2 1 521-5413561 1 9 623-6413562 1 9 4016-40343636 1 8 4480-44973637 1 5 607-6213664 1 5 4000-40143832 4 848-8513833 3 4036-40383913 3 18 -203934 3 643-6453966 1 622-6223967 1 574-5744000 1 642-6424015 1 166-1664016 1 241-2414035 1 242-2424036 1 3832-38324039 1 3636-36364109 1 3561-35614480 1 3966-39664498 1 4035-40354664 1 1332-13324943 1 4015-4015
BseRI 4 540 3095 3932-539GAGGAGNNNNNNNNNN...1360 2533 1399-3931|NNNNNNNNCTCCTC ...1399 820 540-1359
3932 3 9 1360-1398
BsgI 1 2847 6487 2847-2846GTGCAGNNNNNNNNNN...|NNNNNNNNNNNNNNC...
BsiEI 1 2 794 1671 2704-4374CGRY|CG 1727 1410 5871-793
1862 933 794-17261883 923 4799-57211892 570 1892-24612462 424 4375-47982557 149 5722-58702704 147 2557-27034375 135 1727-18614799 9 5 2462-25565722 2 1 1862-18825871 9 1883-1891
BsiHKAI 1 1 414 1389 414-1802GWGCW|C 1803 1161 4777-5937
2105 1030 2105-31343135 878 6023-4133188 515 3292-38063292 498 4279-47763807 472 3807-4278
Sites FragmentsNumber Position Length Ends
4279 302 1803-21044777 104 3188-32915938 8 5 5938-60226023 5 3 3135-3187
BslI 5 6 1 9 1562 4944-18CCNNNNN|NNGG 2 2 587 2794-3380
167 374 2420-2793168 371 4110-4480242 353 853-1205243 287 1803-2089244 279 1461-1739271 279 4665-4943440 221 2199-2419522 202 647-848543 169 271-439575 168 3665-3832576 166 4499-4664608 155 3407-3561623 145 22-166624 127 1334-1460643 109 2090-2198644 8 2 440-521647 8 0 3834-3913849 7 7 1206-1282853 7 4 168-241
1206 7 4 3563-36361283 7 0 4040-41091333 6 3 1740-18021334 5 0 1283-13321461 3 3 3968-40001740 3 2 3935-39661803 3 2 543-5742090 3 2 576-6072199 2 7 3638-36642420 2 7 244-2702794 2 6 3381-34063381 2 1 3914-39343407 2 1 522-5423562 1 9 624-6423563 1 9 4017-40353637 1 8 4481-44983638 1 5 608-6223665 1 5 4001-40153833 4 849-8523834 3 4037-40393914 3 19 -213935 3 644-6463967 1 623-6233968 1 575-5754001 1 643-6434016 1 167-1674017 1 242-242
6Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
4036 1 243-2434037 1 3833-38334040 1 3637-36374110 1 3562-35624481 1 3967-39674499 1 4036-40364665 1 1333-13334944 1 4016-4016
BsmBI 8 800 3185 4102-799CGTCTCN| 898 1622 1587-3208|NNNNNGAGACG 1139 893 3209-4101
1198 241 898-11381396 198 1198-13951587 191 1396-15863209 9 8 800-8974102 5 9 1139-1197
BsmFI 1 1 247 2336 4033-6368GGGACNNNNNNNNNN|...640 1164 1272-2435|NNNNNNNNNNNNNNG...796 548 2722-3269
831 441 831-12711272 393 247-6392436 392 3641-40322722 371 3270-36403270 365 6369-2463641 286 2436-27214033 156 640-7956369 3 5 796-830
Bsp1286 I 1 8 324 1205 598-1802GDGCH|C 414 1161 4777-5937
427 788 6023-323598 643 2105-2747
1803 498 4279-47762105 426 3292-37172748 387 2748-31343135 302 1803-21043188 288 3991-42783292 171 3820-39903718 171 427-5973807 104 3188-32913820 9 0 324-4133991 8 9 3718-38064279 8 5 5938-60224777 5 3 3135-31875938 1 3 414-4266023 1 3 3807-3819
BspEI 4 1519 5593 2413-1518T|CCGGA 1740 537 1740-2276
2277 221 1519-17392413 136 2277-2412
Sites FragmentsNumber Position Length Ends
BspHI 3 5179 5374 6292-5178T|CATGA 6187 1008 5179-6186
6292 105 6187-6291
BspLU11 I 1 4459 6487 4459-4458A|CATGT
BspMI 2 1832 4991 3328-1831ACCTGCNNNN| 3328 1496 1832-3327|NNNNNNNNGCAGGT ...
BsrBI 6 403 2124 2268-4391GAG|CGG 1109 1801 4392-6192CCG|CTC 1910 801 1109-1909
2268 706 403-11084392 697 6193-4026193 358 1910-2267
BsrDI 2 5413 6313 5587-5412GCAATGNN| 5587 174 5413-5586|CATTGC
BsrFI 3 1857 2912 5432-1856R|CCGGY 2699 2733 2699-5431
5432 842 1857-2698
BsrGI 1 1349 6487 1349-1348T|GTACA
BsrI 1 6 449 1672 2170-3841ACTGGN| 1098 938 5998-448C|CAGT 1124 881 1124-2004
2005 649 449-10972170 628 4239-48663842 406 4992-53974206 364 3842-42054239 269 5559-58274867 170 5828-59974880 165 2005-21694992 118 5398-55155398 112 4880-49915516 4 3 5516-55585559 3 3 4206-42385828 2 6 1098-11235998 1 3 4867-4879
BssHII 3 393 3342 444-3785G|CGCGC 444 3094 3786-392
3786 5 1 393-443
BssKI 4 1 160 971 1332-2302|CCNGG 236 763 5884-159
7Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
314 696 4837-5532472 421 813-1233473 400 2740-3139601 351 5533-5883622 347 4138-4484643 321 2419-2739788 257 3298-3554800 218 4619-4836813 158 314-471
1234 157 3708-38641259 145 643-7871282 128 473-6001302 128 3866-39931332 121 4485-46052303 116 2303-24182419 7 8 236-3132740 7 8 3630-37073140 7 6 160-2353141 7 5 3555-36293210 6 9 3141-32093253 6 7 4036-41023297 4 4 3253-32963298 4 3 3210-32523555 3 5 4103-41373630 3 0 1302-13313708 2 5 1234-12583865 2 3 1259-12813866 2 1 601-6213994 2 1 4015-40354015 2 1 622-6424036 2 1 3994-40144103 2 0 1282-13014138 1 3 800-8124485 1 3 4606-46184606 1 2 788-7994619 1 472-4724837 1 3140-31405533 1 3865-38655884 1 3297-3297
BssSI 5 1619 2534 2098-4631C|ACGAG 2098 1783 6323-1618C|TCGTG 4632 1384 4632-6015
6016 479 1619-20976323 307 6016-6322
Bst1107 I 1 4232 6487 4232-4231GTA|TAC
BstEII 1 1154 6487 1154-1153G|GTNACC
BstF5 I 1 1 512 1200 5799-511
Sites FragmentsNumber Position Length Ends
GGATGNN| 1200 1191 4140-5330|CATCC 1380 947 2274-3220
1881 688 512-11992274 683 3221-39033221 501 1380-18803904 393 1881-22734140 287 5512-57985331 236 3904-41395512 181 5331-55115799 180 1200-1379
BstNI 1 6 316 2182 4621-315CC|WGG 624 1408 1334-2741
802 498 3212-3709815 470 2742-3211
1236 470 4017-44861261 421 815-12351284 308 316-6231304 307 3710-40161334 178 624-8012742 121 4487-46073212 3 0 1304-13333710 2 5 1236-12604017 2 3 1261-12834487 2 0 1284-13034608 1 3 802-8144621 1 3 4608-4620
BstUI 3 1 393 583 2691-3273CG|CG 395 581 4506-5086
446 524 446-969970 512 3274-3785972 493 5417-5909
1202 413 6467-3921540 341 4165-45051729 338 1202-15391864 332 5910-62411975 330 5087-54162041 272 3788-40592107 230 972-12012256 189 1540-17282298 165 6242-64062458 160 2298-24572599 149 2107-22552601 141 2458-25982691 135 1729-18633274 111 1864-19743786 103 4062-41643788 9 0 2601-26904060 6 6 2041-21064062 6 6 1975-20404165 6 0 6407-64664506 5 1 395-445
8Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
5087 4 2 2256-22975417 2 393-3945910 2 970-9716242 2 2599-26006407 2 4060-40616467 2 3786-3787
BstYI 9 1516 2009 5994-1515R|GATCY 2830 1797 3303-5099
3303 1314 1516-28295100 768 5209-59765111 473 2830-33025197 8 6 5111-51965209 1 7 5977-59935977 1 2 5197-52085994 1 1 5100-5110
Bsu36 I 2 848 6250 1085-847CC|TNAGG 1085 237 848-1084
Cac8 I 3 2 2 8 812 5597-6408GCN|NGC 3 2 560 4513-5072
395 549 717-1265446 510 2815-3324657 391 5073-5463661 366 3422-3787717 363 32-394
1266 355 1489-18431489 340 4050-43891844 262 3788-40492045 223 1266-14882105 211 446-6562285 201 1844-20442448 180 2105-22842590 163 2285-24472603 142 2448-25892676 139 2676-28142815 133 5464-55963325 106 6409-273329 8 6 4390-44753333 8 5 3333-34173418 7 3 2603-26753422 6 0 2045-21043788 5 6 661-7164050 5 1 395-4454390 3 7 4476-45124476 1 3 2590-26024513 4 28 -315073 4 657-6605464 4 3325-33285597 4 3418-34216409 4 3329-3332
Sites FragmentsNumber Position Length Ends
ClaI 1 3346 6487 3346-3345AT|CGAT
Csp6 I 1 3 347 1565 4266-5830G|TAC 478 1003 5831-346
725 934 1501-24341350 650 2488-31371501 625 725-13492435 576 3295-38702488 395 3871-42653138 247 478-7243159 151 1350-15003295 136 3159-32943871 131 347-4774266 5 3 2435-24875831 2 1 3138-3158
CviJ I 125 3 0 587 5742-6328RG|CY 3 4 415 2718-3132
120 363 5020-5382165 171 3818-3988172 144 4238-4381191 132 280-411203 131 3674-3804241 128 1919-2046248 126 841-966280 124 1054-1177412 9 8 498-595425 9 8 4529-4626498 9 0 4627-4716596 9 0 2477-2566655 9 0 5541-5630659 8 7 3424-3510735 8 7 967-1053743 8 6 34-119793 8 5 1361-1445829 8 5 3335-3419841 8 2 3186-3267967 8 2 1775-1856
1054 8 2 2144-22251178 8 2 6329-64101199 7 9 4793-48711264 7 6 659-7341311 7 3 4401-44731317 7 3 425-4971343 7 2 1527-15981361 6 6 5475-55401446 6 5 4872-49361487 6 5 1199-12631527 6 3 5641-57031599 5 9 3989-40471631 5 9 596-6541673 5 3 1673-1725
9Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
1726 5 0 743-7921775 4 9 4052-41001857 4 9 1726-17741870 4 7 6470-291891 4 7 2301-23471901 4 7 1264-13101919 4 7 2236-22822047 4 7 6411-64572058 4 6 2071-21162071 4 6 4717-47622117 4 5 3290-33342144 4 5 120-1642226 4 5 4153-41972236 4 3 4948-49902283 4 2 2377-24182301 4 2 2419-24602348 4 2 2632-26732377 4 2 1631-16722419 4 1 1446-14862461 4 1 5395-54352477 4 0 4198-42372567 4 0 1487-15262588 3 8 203-2402605 3 8 5704-57412632 3 8 3133-31702674 3 8 3522-35592683 3 8 3597-36342699 3 6 793-8282718 3 3 4120-41523133 3 2 248-2793171 3 2 1599-16303186 3 2 3642-36733268 2 9 2348-23763277 2 9 4991-50193290 2 7 2117-21433335 2 7 2605-26313420 2 6 4503-45283424 2 6 1317-13423511 2 6 5436-54613522 2 5 4768-47923560 2 1 1870-18903567 2 1 2567-25873586 2 1 1178-11983597 1 9 4382-44003635 1 9 4101-41193642 1 9 2699-27173674 1 9 172-1903805 1 9 3567-35853818 1 8 4485-45023989 1 8 1343-13604048 1 8 2283-23004052 1 8 1901-19184101 1 7 2588-2604
Sites FragmentsNumber Position Length Ends
4120 1 6 2683-26984153 1 6 2461-24764198 1 5 3171-31854238 1 3 3805-38174382 1 3 412-4244401 1 3 3277-32894474 1 3 1857-18694485 1 3 2058-20704503 1 2 191-2024529 1 2 5383-53944627 1 2 829-8404717 1 1 3511-35214763 1 1 4937-49474768 1 1 4474-44844793 1 1 3586-35964872 1 1 2047-20574937 1 0 1891-19004948 1 0 5631-56404991 1 0 2226-22355020 9 5466-54745383 9 3268-32765395 9 2674-26825436 8 735-7425462 7 241-2475466 7 165-1715475 7 3635-36415541 7 3560-35665631 6 6458-64635641 6 6464-64695704 6 1311-13165742 5 4763-47676329 4 30 -336411 4 655-6586458 4 3420-34236464 4 5462-54656470 4 4048-4051
DdeI 2 4 7 6 796 2670-3465C|TNAG 166 687 1897-2583
192 540 1357-1896283 540 5309-5848554 465 4269-4733848 426 5849-6274
1085 409 4734-51421357 323 3946-42681897 294 554-8472584 272 1085-13562670 271 283-5533466 237 848-10843561 181 3677-38573636 166 5143-53083677 146 6275-64203858 142 6421-75
1 0Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
3946 9 5 3466-35604269 9 1 192-2824734 9 0 76-1655143 8 8 3858-39455309 8 6 2584-26695849 7 5 3561-36356275 4 1 3636-36766421 2 6 166-191
DpnI 3 6 9 6 1027 4000-5026GA|TC 1113 1017 96-1112
1245 514 3486-39991430 375 2832-32061479 364 2412-27751518 341 5316-56561564 326 6032-63571697 307 1985-22911882 258 5721-59781895 225 6358-951985 185 1245-14292292 185 1697-18812412 181 3305-34852776 133 1564-16962785 132 1113-12442801 120 2292-24112832 105 5211-53153207 9 0 1895-19843263 7 8 5121-51983305 7 5 5027-51013486 5 6 3207-32624000 4 9 1430-14785027 4 6 5675-57205102 4 6 1518-15635113 4 2 3263-33045121 3 9 1479-15175199 3 6 5996-60315211 3 1 2801-28315316 1 8 5657-56745657 1 7 5979-59955675 1 6 2785-28005721 1 3 1882-18945979 1 2 5199-52105996 1 1 5102-51126032 9 2776-27846358 8 5113-5120
DraI 3 5218 5776 5929-5217TTT|AAA 5237 692 5237-5928
5929 1 9 5218-5236
DraIII 2 1807 6194 2100-1806CACNNN|GTG 2100 293 1807-2099
Sites FragmentsNumber Position Length Ends
DrdI 4 2023 3943 4567-2022GACNNNN|NNGTC 2404 1750 2404-4153
4154 413 4154-45664567 381 2023-2403
DsaI 6 761 3193 3272-6464C|CRYGG 1871 1110 761-1870
2227 976 2296-32712296 783 6465-7603272 356 1871-22266465 6 9 2227-2295
EaeI 9 657 3281 2459-5739Y|GGCCR 791 1404 5740-656
1176 527 1197-17231197 385 791-11751724 317 2142-24581889 253 1889-21412142 165 1724-18882459 134 657-7905740 2 1 1176-1196
EagI 4 791 4819 2459-790C|GGCCG 1724 933 791-1723
1889 570 1889-24582459 165 1724-1888
EarI 4 1137 2965 1378-4342CTCTTCN| 1378 1804 4343-6146|NNNNGAAGAG 4343 1477 6147-1136
6147 241 1137-1377
Ecl136 II 5 412 3094 3805-411GAG|CTC 3133 2721 412-3132
3186 515 3290-38043290 104 3186-32893805 5 3 3133-3185
Eco47 III 1 990 6487 990-989AGC|GCT
Eco57 I 2 5007 5475 6019-5006CTGAAGNNNNNNNNNN...6019 1012 5007-6018|NNNNNNNNNNNNNNC...
EcoNI 2 851 5879 1459-850CCTNN|NNNAGG 1459 608 851-1458
EcoO109 I 5 329 2605 3723-6327RG|GNCCY 1286 1431 1286-2716
2717 1006 2717-37223723 957 329-12856328 488 6328-328
1 1Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
EcoRI 4 1471 3063 3282-6344G|AATTC 1762 1613 6345-1470
3282 1520 1762-32816345 291 1471-1761
EcoRII 1 6 314 2182 4619-313|CCWGG 622 1408 1332-2739
800 498 3210-3707813 470 2740-3209
1234 470 4015-44841259 421 813-12331282 308 314-6211302 307 3708-40141332 178 622-7992740 121 4485-46053210 3 0 1302-13313708 2 5 1234-12584015 2 3 1259-12814485 2 0 1282-13014606 1 3 800-8124619 1 3 4606-4618
EcoRV 4 138 3319 214-3532GAT|ATC 214 3017 3608-137
3533 7 6 138-2133608 7 5 3533-3607
EheI 2 1466 4113 3840-1465GGC|GCC 3840 2374 1466-3839
FauI 1 4 934 2226 4174-6399CCCGCNNNN| 1273 1166 2795-3960|NNNNNNGCGGG 1429 1021 6400-933
1655 552 1903-24541801 339 934-12721851 229 2455-26831903 226 1429-16542455 203 3961-41632684 156 1273-14282795 146 1655-18003961 111 2684-27944164 5 2 1851-19024174 5 0 1801-18506400 1 0 4164-4173
Fnu4HI 4 2 794 813 6468-793GC|NGC 968 778 3275-4052
984 470 2805-3274987 397 1200-1596992 371 6094-6464995 346 2459-2804
1200 328 5076-5403
Sites FragmentsNumber Position Length Ends
1597 256 1889-21441674 229 5865-60931727 206 4870-50751855 205 995-11991886 189 5404-55921889 174 794-9672145 155 4504-46582248 150 5593-57422296 143 4659-48012299 128 1727-18542375 118 4386-45032459 116 4249-43642805 103 2145-22473275 9 7 4099-41954053 9 5 5770-58644099 8 4 2375-24584196 7 7 1597-16734249 7 6 2299-23744365 6 5 4802-48664383 5 3 4196-42484386 5 3 1674-17264504 4 8 2248-22954659 4 6 4053-40984802 3 1 1855-18854867 2 7 5743-57694870 1 8 4365-43825076 1 6 968-9835404 5 987-9915593 3 6465-64675743 3 984-9865770 3 4867-48695865 3 2296-22986094 3 4383-43856465 3 1886-18886468 3 992-994
FokI 1 1 499 1200 5786-498GGATGNNNNNNNNN| ...1207 1171 4147-5317|NNNNNNNNNNNNNCA...1367 947 2261-3207
1888 708 499-12062261 683 3208-38903208 521 1367-18873891 373 1888-22604147 287 5499-57855318 256 3891-41465499 181 5318-54985786 160 1207-1366
FspI 1 5574 6487 5574-5573TGC|GCA
HaeII 5 992 2772 4707-991RGCGC|Y 1468 2374 1468-3841
1 2Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
3842 495 3842-43364337 476 992-14674707 370 4337-4706
HaeIII 3 7 172 832 3642-4473GG|CC 203 587 5742-6328
248 462 1264-1725659 458 4937-5394793 453 2718-3170829 434 4503-4936841 411 248-658
1054 267 5475-57411178 245 3277-35211199 235 2226-24601264 213 841-10531726 189 6470-1711870 171 2461-26311891 152 1919-20701919 144 1726-18692071 141 6329-64692144 134 659-7922226 124 1054-11772461 106 3171-32762632 8 2 2144-22252699 8 0 5395-54742718 7 3 2071-21433171 6 7 2632-26983277 6 5 1199-12633522 4 5 3597-36413567 4 5 203-2473597 4 5 3522-35663642 3 6 793-8284474 3 1 172-2024485 3 0 3567-35964503 2 8 1891-19184937 2 1 1870-18905395 2 1 1178-11985475 1 9 2699-27175742 1 8 4485-45026329 1 2 829-8406470 1 1 4474-4484
HgaI 1 3 456 1065 5878-455GACGCNNNNN| 708 928 3226-4153|NNNNNNNNNNGCGTC...961 730 5148-5877
1569 707 2519-32252021 608 961-15682262 578 4570-51472407 452 1569-20202519 416 4154-45693226 253 708-9604154 252 456-7074570 241 2021-2261
Sites FragmentsNumber Position Length Ends
5148 145 2262-24065878 112 2407-2518
HhaI 3 3 395 1187 2601-3787GCG|C 397 638 6244-394
446 476 991-1466448 393 5089-5481715 337 5575-5911972 332 5912-6243991 270 4369-4638
1467 267 448-7141678 257 715-9711731 244 1731-19741975 221 3841-40612043 215 2043-22572258 211 1467-16772385 174 4806-49792458 143 2458-26002601 141 4195-43353788 127 2258-23843790 109 4980-50883841 103 4062-41644062 100 4706-48054165 9 3 5482-55744195 7 3 2385-24574336 6 8 1975-20424369 6 7 4639-47054639 5 3 1678-17304706 5 1 3790-38404806 4 9 397-4454980 3 3 4336-43685089 3 0 4165-41945482 1 9 972-9905575 2 395-3965912 2 446-4476244 2 3788-3789
HinP1 I 3 3 393 1187 2599-3785G|CGC 395 638 6242-392
444 476 989-1464446 393 5087-5479713 337 5573-5909970 332 5910-6241989 270 4367-4636
1465 267 446-7121676 257 713-9691729 244 1729-19721973 221 3839-40592041 215 2041-22552256 211 1465-16752383 174 4804-49772456 143 2456-25982599 141 4193-4333
1 3Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
3786 127 2256-23823788 109 4978-50863839 103 4060-41624060 100 4704-48034163 9 3 5480-55724193 7 3 2383-24554334 6 8 1973-20404367 6 7 4637-47034637 5 3 1676-17284704 5 1 3788-38384804 4 9 395-4434978 3 3 4334-43665087 3 0 4163-41925480 1 9 970-9885573 2 393-3945910 2 444-4456242 2 3786-3787
HincII 4 2355 2949 5893-2354GTY|RAC 3317 2552 3341-5892
3341 962 2355-33165893 2 4 3317-3340
HindIII 1 3333 6487 3333-3332A|AGCTT
HinfI 1 5 299 1439 5347-298G|ANTC 518 746 869-1614
858 602 2711-3312869 547 3313-3859
1615 523 2188-27101737 517 4830-53461937 499 3860-43582188 396 4434-48292711 340 518-8573313 251 1937-21873860 219 299-5174359 200 1737-19364434 122 1615-17364830 7 5 4359-44335347 1 1 858-868
HpaI 1 3341 6487 3341-3340GTT|AAC
HpaII 3 6 162 678 790-1467C|CGG 238 567 5886-6452
474 527 4139-4665602 442 2700-3141644 404 5029-5432790 280 2420-2699
1468 258 3299-35561520 242 5644-5885
Sites FragmentsNumber Position Length Ends
1741 236 238-4731858 235 3632-38662091 233 1858-20902278 221 1520-17402304 196 6453-1612414 190 4839-50282420 187 2091-22772700 147 4666-48123142 146 644-7893254 128 474-6013299 128 3867-39943557 117 1741-18573632 112 3142-32533867 110 5534-56433995 110 2304-24134037 7 6 162-2374105 7 5 3557-36314139 6 8 4037-41044666 6 7 5467-55334813 5 2 1468-15194839 4 5 3254-32985029 4 2 602-6435433 4 2 3995-40365467 3 4 4105-41385534 3 4 5433-54665644 2 6 4813-48385886 2 6 2278-23036453 6 2414-2419
HphI 1 3 1223 1297 2784-4080GGTGANNNNNNNN| 1249 1268 6442-1222|NNNNNNNTCACC 1527 1257 1527-2783
2784 1105 4090-51944081 416 5422-58374090 278 1249-15265195 272 6079-63505422 227 5195-54215838 206 5838-60436044 9 1 6351-64416079 3 5 6044-60786351 2 6 1223-12486442 9 4081-4089
KasI 2 1464 4113 3838-1463G|GCGCC 3838 2374 1464-3837
KpnI 4 481 3094 3874-480GGTAC|C 3141 2660 481-3140
3298 576 3298-38733874 157 3141-3297
MaeII 1 0 809 2399 1813-4211A|CGT 1148 1025 6271-808
1 4Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
1189 950 4212-51611543 416 5162-55771813 373 5578-59504212 354 1189-15425162 339 809-11475578 320 5951-62705951 270 1543-18126271 4 1 1148-1188
MaeIII 1 7 3 9 1620 1809-3428|GTNAC 1061 1022 39-1060
1089 684 3429-41121154 607 4208-48141298 519 6007-381809 511 1298-18083429 331 5277-56074113 283 4994-52764208 188 5819-60064815 153 5666-58184878 144 1154-12974994 116 4878-49935277 9 5 4113-42075608 6 5 1089-11535666 6 3 4815-48775819 5 8 5608-56656007 2 8 1061-1088
MboII 1 4 445 1071 3259-4329GAAGANNNNNNNN| 1154 1051 2208-3258|NNNNNNNTCTTC 1365 791 4330-5120
1624 755 5192-59462205 709 445-11532208 602 6330-4443259 581 1624-22044330 259 1365-16235121 211 1154-13645192 196 6134-63295947 109 6025-61336025 7 8 5947-60246134 7 1 5121-51916330 3 2205-2207
Mnl I 5 6 292 594 5725-6318CCTCNNNNNNN| 445 473 2688-3160|NNNNNNGAGG 509 400 4891-5290
536 369 3317-3685561 351 6428-291641 296 940-1235843 262 4096-4357940 258 2264-2521
1236 250 4641-48901339 224 1636-18591372 209 4358-4566
Sites FragmentsNumber Position Length Ends
1378 207 1860-20661381 206 5519-57241387 202 641-8421417 153 292-4441420 152 3686-38371435 147 5372-55181442 141 1458-15981458 103 1236-13381599 9 7 843-9391636 9 5 2593-26871860 8 8 3161-32482067 8 1 5291-53712107 8 1 3953-40332166 8 0 561-6402193 7 4 4567-46402216 7 1 2522-25922264 6 4 6319-63822522 6 4 445-5082593 5 9 2107-21652688 4 8 2216-22633161 4 5 3857-39013249 4 0 2067-21063279 3 8 3279-33163317 3 7 1599-16353686 3 3 1339-13713838 3 2 4034-40653857 3 0 1387-14163902 3 0 3249-32783928 3 0 4066-40953953 2 9 6399-64274034 2 7 509-5354066 2 7 2166-21924096 2 6 3902-39274358 2 5 536-5604567 2 5 3928-39524641 2 3 2193-22154891 1 9 3838-38565291 1 6 1442-14575372 1 6 6383-63985519 1 5 1420-14345725 7 1435-14416319 6 1381-13866383 6 1372-13776399 3 1378-13806428 3 1417-1419
MscI 2 659 5968 1178-658TGG|CCA 1178 519 659-1177
MseI 1 7 3 6 2100 1240-3339T|TAA 1074 1038 36-1073
1186 925 4240-51641216 814 3426-4239
1 5Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
1240 372 5928-62993340 365 5563-59273426 235 5289-55234240 223 6300-355165 112 1074-11855217 8 6 3340-34255222 5 3 5236-52885236 5 2 5165-52165289 3 9 5524-55625524 3 0 1186-12155563 2 4 1216-12395928 1 4 5222-52356300 5 5217-5221
Msl I 4 2770 3135 6122-2769CAYNN|NNRTG 5604 2834 2770-5603
5763 359 5763-61216122 159 5604-5762
MspA1 I 1 4 120 1655 191-1845CMG|CKG 191 976 2298-3273
1846 941 5046-59862155 630 4171-48002298 480 5987-64663274 382 3586-39673511 309 1846-21543586 245 4801-50453968 237 3274-35104171 203 3968-41704801 143 2155-22975046 140 6467-1195987 7 5 3511-35856467 7 1 120-190
MwoI 3 3 156 1317 409-1725GCNNNNN|NNGC 232 872 5592-6463
409 572 4512-50831726 481 3802-42821793 416 2810-32251824 388 5084-54711876 277 3274-35501885 218 2380-25971907 179 6464-1551916 177 232-4082038 176 3626-38012100 153 2100-22522253 130 2680-28092280 122 1916-20372380 120 5472-55912598 114 4398-45112607 100 2280-23792675 7 6 156-2312680 7 5 3551-3625
Sites FragmentsNumber Position Length Ends
2810 6 8 2607-26743226 6 7 1726-17923274 6 5 4333-43973551 6 2 2038-20993626 5 2 1824-18753802 5 0 4283-43324283 4 8 3226-32734333 3 1 1793-18234398 2 7 2253-22794512 2 2 1885-19065084 9 2598-26065472 9 1907-19155592 9 1876-18846464 5 2675-2679
NarI 2 1465 4113 3839-1464GG|CGCC 3839 2374 1465-3838
NciI 2 5 162 1515 790-2304CC|SGG 238 763 5886-161
474 721 2421-3141475 699 4140-4838603 696 4839-5534645 351 5535-5885790 257 3300-3556
2305 236 238-4732421 235 3632-38663142 145 645-7893143 128 3868-39953255 128 475-6023299 116 2305-24203300 112 3143-32543557 7 6 162-2373632 7 5 3557-36313867 6 7 4038-41043868 4 4 3255-32983996 4 2 603-6444038 4 2 3996-40374105 3 5 4105-41394140 1 474-4744839 1 3867-38675535 1 3299-32995886 1 3142-3142
NcoI 1 1871 6487 1871-1870C|CATGG
NdeI 2 1969 4174 4282-1968CA|TATG 4282 2313 1969-4281
NheI 2 3 0 3390 30-3419G|CTAGC 3420 3097 3420-29
1 6Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
NlaIII 1 8 6 2 1210 62-1271CATG| 1272 766 1993-2758
1875 720 4463-51821958 646 3452-40971993 603 1272-18742759 572 2759-33303331 491 5183-56733452 393 5798-61904098 260 4203-44624203 253 6296-614463 121 3331-34515183 105 6191-62955674 105 4098-42025684 8 3 1875-19575762 7 8 5684-57615798 3 6 5762-57976191 3 5 1958-19926296 1 0 5674-5683
NlaIV 3 5 154 772 4530-5301GGN|NCC 247 629 1466-2094
263 590 5648-6237321 470 4021-4490479 401 2738-3138628 352 3305-3656759 320 2418-2737828 251 960-1210960 219 6238-6456
1211 211 5437-56471288 184 2118-23011466 184 6457-1532095 178 1288-14652118 158 321-4782302 149 479-6272418 149 3872-40202738 132 828-9593139 131 628-7583258 125 3715-38393278 119 3139-32573296 116 2302-24173305 9 4 5302-53953657 9 3 154-2463715 7 7 1211-12873840 6 9 759-8273872 5 8 263-3204021 5 8 3657-37144491 4 1 5396-54364530 3 9 4491-45295302 3 2 3840-38715396 2 3 2095-21175437 2 0 3258-32775648 1 8 3278-32956238 1 6 247-262
Sites FragmentsNumber Position Length Ends
6457 9 3296-3304
NspI 3 3331 5355 4463-3330RCATG|Y 4098 767 3331-4097
4463 365 4098-4462
PleI 6 866 2455 866-3320GAGTCNNNN| 3321 2012 5341-865|NNNNNGACTC 3868 547 3321-3867
4353 503 4838-53404838 485 3868-43525341 485 4353-4837
PpuMI 3 329 3093 3723-328RG|GWCCY 1286 2437 1286-3722
3723 957 329-1285
PshAI 2 578 5519 1546-577GACNN|NNGTC 1546 968 578-1545
Psp1406 I 2 5578 6114 5951-5577AA|CGTT 5951 373 5578-5950
PstI 6 988 2272 3325-5596CTGCA|G 996 1878 5597-987
1172 1469 1856-33241856 684 1172-18553325 176 996-11715597 8 988-995
PvuI 2 1883 3839 1883-5721CGAT|CG 5722 2648 5722-1882
PvuII 4 120 3320 191-3510CAG|CTG 191 3021 3586-119
3511 7 5 3511-35853586 7 1 120-190
RsaI 1 3 348 1565 4267-5831GT|AC 479 1003 5832-347
726 934 1502-24351351 650 2489-31381502 625 726-13502436 576 3296-38712489 395 3872-42663139 247 479-7253160 151 1351-15013296 136 3160-32953872 131 348-4784267 5 3 2436-24885832 2 1 3139-3159
RsrII 1 1927 6487 1927-1926
1 7Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
CG|GWCCG
SacI 5 414 3094 3807-413GAGCT|C 3135 2721 414-3134
3188 515 3292-38063292 104 3188-32913807 5 3 3135-3187
SacII 3 2299 3193 3275-6467CCGC|GG 3275 2318 6468-2298
6468 976 2299-3274
Sal I 1 3315 6487 3315-3314G|TCGAC
SapI 1 4343 6487 4343-4342GCTCTTCN||NNNNGAAGAGC
Sau3AI 3 6 9 4 1027 3998-5024|GATC 1111 1017 94-1110
1243 514 3484-39971428 375 2830-32041477 364 2410-27731516 341 5314-56541562 326 6030-63551695 307 1983-22891880 258 5719-59761893 225 6356-931983 185 1243-14272290 185 1695-18792410 181 3303-34832774 133 1562-16942783 132 1111-12422799 120 2290-24092830 105 5209-53133205 9 0 1893-19823261 7 8 5119-51963303 7 5 5025-50993484 5 6 3205-32603998 4 9 1428-14765025 4 6 5673-57185100 4 6 1516-15615111 4 2 3261-33025119 3 9 1477-15155197 3 6 5994-60295209 3 1 2799-28295314 1 8 5655-56725655 1 7 5977-59935673 1 6 2783-27985719 1 3 1880-18925977 1 2 5197-52085994 1 1 5100-5110
Sites FragmentsNumber Position Length Ends
6030 9 2774-27826356 8 5111-5118
Sau96 I 3 6 170 1374 4020-5393G|GNCC 201 632 1286-1917
246 616 5712-6327261 521 2736-3256274 399 2069-2467329 298 329-626627 297 3723-4019757 244 3276-3519827 229 2468-2696840 222 5490-5711
1052 212 840-10511263 211 1052-12621286 188 6469-1691918 142 1927-20681927 141 6328-64682069 130 627-7562468 7 9 5394-54722697 7 0 757-8262717 5 5 3668-37222736 5 5 274-3283257 4 5 3520-35643276 4 5 3595-36393520 4 5 201-2453565 3 1 170-2003595 3 0 3565-35943640 2 3 1263-12853655 2 0 2697-27163668 1 9 2717-27353723 1 9 3257-32754020 1 7 5473-54895394 1 5 246-2605473 1 5 3640-36545490 1 3 261-2735712 1 3 3655-36676328 1 3 827-8396469 9 1918-1926
ScaI 2 2489 3343 2489-5831AGT|ACT 5832 3144 5832-2488
ScrFI 4 1 162 971 1334-2304CC|NGG 238 763 5886-161
316 696 4839-5534474 421 815-1235475 400 2742-3141603 351 5535-5885624 347 4140-4486645 321 2421-2741790 257 3300-3556802 218 4621-4838
1 8Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
815 158 316-4731236 157 3710-38661261 145 645-7891284 128 475-6021304 128 3868-39951334 121 4487-46072305 116 2305-24202421 7 8 238-3152742 7 8 3632-37093142 7 6 162-2373143 7 5 3557-36313212 6 9 3143-32113255 6 7 4038-41043299 4 4 3255-32983300 4 3 3212-32543557 3 5 4105-41393632 3 0 1304-13333710 2 5 1236-12603867 2 3 1261-12833868 2 1 603-6233996 2 1 4017-40374017 2 1 624-6444038 2 1 3996-40164105 2 0 1284-13034140 1 3 802-8144487 1 3 4608-46204608 1 2 790-8014621 1 474-4744839 1 3142-31425535 1 3867-38675886 1 3299-3299
SexAI 1 1282 6487 1282-1281A|CCWGGT
SfaNI 2 2 259 1114 2539-3652GCATCNNNNN| 521 1052 4556-5607|NNNNNNNNNGATGC ...1006 698 6048-258
1583 577 1006-15821729 485 521-10051796 262 259-5201866 260 3653-39122051 249 5799-60472283 232 2051-22822361 220 4336-45552383 212 3913-41242539 191 5608-57983653 185 1866-20503913 156 2383-25384125 146 1583-17284260 135 4125-42594296 7 8 2283-23604336 7 0 1796-1865
Sites FragmentsNumber Position Length Ends
4556 6 7 1729-17955608 4 0 4296-43355799 3 6 4260-42956048 2 2 2361-2382
SfcI 9 984 1878 5593-983C|TRYAG 992 1469 1852-3320
1168 1319 3405-47231852 684 1168-18513321 678 4915-55923405 191 4724-49144724 176 992-11674915 8 4 3321-34045593 8 984-991
SgfI 1 1883 6487 1883-1882GCGAT|CGC
SmaI 4 475 3094 3868-474CCC|GGG 3143 2668 475-3142
3300 568 3300-38673868 157 3143-3299
SpeI 1 727 6487 727-726A|CTAGT
SphI 1 3331 6487 3331-3330GCATG|C
Sse8387 I 1 3325 6487 3325-3324CCTGCA|GG
SspI 1 6156 6487 6156-6155AAT|ATT
StuI 1 3171 6487 3171-3170AGG|CCT
StyI 6 325 2883 3929-324C|CWWGG 537 1848 1871-3718
1312 775 537-13111871 559 1312-18703719 212 325-5363929 210 3719-3928
TaiI 1 0 812 2399 1816-4214ACGT| 1151 1025 6274-811
1192 950 4215-51641546 416 5165-55801816 373 5581-59534215 354 1192-15455165 339 812-11505581 320 5954-6273
1 9Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
5954 270 1546-18156274 4 1 1151-1191
TaqI 2 7 861 1444 4559-6002T|CGA 1105 1345 6003-860
1416 1213 3346-45581427 417 1634-20501550 311 1105-14151565 261 2574-28341574 244 861-11041634 213 2051-22632051 183 2391-25732264 139 3147-32852366 123 1427-15492391 102 2264-23652574 6 0 1574-16332835 4 2 3045-30862877 4 2 3003-30442919 4 2 3087-31282961 4 2 2835-28763003 4 2 2877-29183045 4 2 2961-30023087 4 2 2919-29603129 3 0 3286-33153147 3 0 3316-33453286 2 5 2366-23903316 1 8 3129-31463346 1 5 1550-15644559 1 1 1416-14266003 9 1565-1573
Tf i I 9 299 2352 4434-298G|AWTC 518 1723 2711-4433
869 746 869-16141615 523 2188-27101737 351 518-8681937 251 1937-21872188 219 299-5172711 200 1737-19364434 122 1615-1736
TseI 2 2 983 1701 5769-982G|CWGC 986 1678 2374-4051
991 602 994-1595994 419 4382-4800
1596 362 1885-22461673 328 5075-54021854 206 4869-50741885 189 5403-55912247 181 1673-18532374 177 5592-57684052 169 4195-43634098 127 2247-2373
Sites FragmentsNumber Position Length Ends
4195 9 7 4098-41944364 7 7 1596-16724382 6 5 4801-48654801 4 6 4052-40974866 3 1 1854-18844869 1 8 4364-43815075 5 986-9905403 3 983-9855592 3 991-9935769 3 4866-4868
Tsp45 I 7 1089 2304 1809-4112|GTSAC 1298 1757 5819-1088
1809 1400 4208-56074113 511 1298-18084208 211 5608-58185608 209 1089-12975819 9 5 4113-4207
Tsp509 I 1 1 939 1937 3282-5218|AATT 1046 1025 6401-938
1471 921 2361-32811762 599 1762-23602361 565 5780-63443282 425 1046-14705219 306 5219-55245525 291 1471-17615780 255 5525-57796345 107 939-10456401 5 6 6345-6400
TspRI 1 9 1098 1806 5779-1097CAGTGNN| 2005 1248 3113-4360CACTGNN| 2034 907 1098-2004
2713 679 2034-27122861 506 4361-48662903 347 5405-57512945 271 4880-51502987 149 5151-52993029 148 2713-28603071 105 5300-54043113 4 2 2903-29444361 4 2 2945-29864867 4 2 2861-29024880 4 2 3071-31125151 4 2 2987-30285300 4 2 3029-30705405 2 9 2005-20335752 2 7 5752-57785779 1 3 4867-4879
Tth111 I 7 466 2747 4206-465GACN|NNGTC 878 1836 2023-3858
2 0Digest 2 of pRevTRE: 6487 bases (circular)Tue, Sep 26, 2000 Page
Sites FragmentsNumber Position Length Ends
1284 444 1579-20221579 412 466-8772023 406 878-12833859 347 3859-42054206 295 1284-1578
XbaI 2 3309 6109 3687-3308T|CTAGA 3687 378 3309-3686
XhoI 1 2834 6487 2834-2833C|TCGAG
XmaI 4 473 3094 3866-472C|CCGGG 3141 2668 473-3140
3298 568 3298-38653866 157 3141-3297
XmnI 1 5951 6487 5951-5950GAANN|NNTTC
1Page Sequence Analysis, Tue, Sep 26, 2000
The following restriction enzymes do not cut pRevTRE:
AgeI, ApaI, AvrII, Bcl I, BsaBI, BsiWI, BsmI, Bsp120 I, BstBI, BstXI,
FseI, MfeI, MluI, NaeI, NgoMI, NotI, NruI, NsiI, PacI, PflMI,
PmeI, Pml I, Ppu10 I, SfiI, SgrAI, SnaBI, SrfI, SwaI, XcmI.
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