carbon and nitrogen isotopic composition of hot spring biofilms from yellowstone national park
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Carbon and nitrogen isotopic composition of hot spring biofilms from Yellowstone
National Park
Joseph Murray
Mentor: Dr. Ariel Anbar
Astrobiology at Yellowstone National Park
• Yellowstone abounds with diverse hydrothermal ecosystems
• Provides an excellent opportunity to observe many unique organisms living in extreme habitats
• Unique chance to search for variations in the elemental requirements for life– Biological Stoichiometry
Jeff Havig, Ph.D. Candidate
What is a bioflim?
Isotope fractionation
H2O12C 12C
14N 15N
Nutrients
Bison Pool
Source Flow Direction
High Temperature Low Temperature
93º C 67º C 50º C
“Photosynthetic Fringe”
-25
-20
-15
-10
-5
0
50 60 70 80 90 100Temperature (C)
D13 C
fro
m D
IC (
‰)
2005 Biofilm
2006 Biofilm
Ph
oto
syn
thet
ic F
rin
ge
This plot shows the D13C (fractionation from DIC) values for biofilms from ‘Bison Pool’ collected in 2005 and 2006. The lines and arrows are put in place only as estimates of averages and to help illustrate the assumed trend in fractionation.
Carbon Cycling In Bison Pool: 2005-2006
(J. Havig, in prep.)
-25
-20
-15
-10
-5
0
50 60 70 80 90 100Temperature (C)
D13 C
fro
m D
IC (
‰)
2005 Biofilm
2006 Biofilm
Ph
oto
syn
thet
ic F
rin
ge
This plot shows the D13C (fractionation from DIC) values for biofilms from ‘Bison Pool’ collected in 2005, 2006 and 2007. The lines and arrows are put in place only as estimates of averages and to help illustrate the assumed trend in fractionation.
2007 Biofilm
Carbon Cycling In Bison Pool: 2005-2007
(J. Havig, in prep.)
-25
-20
-15
-10
-5
0
50 60 70 80 90 100Temperature (C)
D13 C
fro
m D
IC (
‰)
2005 Biofilm
2006 Biofilm
Ph
oto
syn
thet
ic F
rin
ge
This plot shows the D13C (fractionation from DIC) values for biofilms from ‘Bison Pool’ collected in 2005, 2006 and 2007. The lines and arrows are put in place only as estimates of averages and to help illustrate the assumed trend in fractionation.
2007 Biofilm
Carbon Cycling In Bison Pool: 2005-2007
(J. Havig, in prep.)
TCA
PEP
-2
0
2
4
6
8
10
50 60 70 80 90 100Temperature (oC)
d15 N
vs
air
(‰)
Grasses
Sinter SoilInsects
Sedges
Bison Exc.
Bison Fur
Soil
Ph
oto
syn
thet
ic F
rin
ge
2006 Biofilm
Nitrogen Cycling In Bison Pool: 2006
(J. Havig, in prep.)
Nitrogen Cycling In Bison Pool: 2006-2007
(J. Havig, in prep.)
-2
0
2
4
6
8
10
50 60 70 80 90 100Temperature (oC)
d15 N
vs
air
(‰)
Grasses
Sinter SoilInsects
Sedges
Bison Exc.
Bison Fur
Soil
Ph
oto
syn
thet
ic F
rin
ge
2006 Biofilm
2007 Biofilm
Year-to-year variability?
-2
0
2
4
6
8
10
50 60 70 80 90 100Temperature (oC)
d15 N
vs
air
(‰)
Bison Exc.
Soil
Ph
oto
syn
thet
ic F
rin
ge Spent Kleenex
Spring
-25
-20
-15
-10
-5
0
50 60 70 80 90 100Temperature (C)
D13 C
fro
m D
IC (
‰)
Ph
oto
syn
thet
ic F
rin
ge Octopus Spring
-2
0
2
4
6
8
10
50 60 70 80 90 100Temperature (oC)
d15 N
vs
air
(‰)
Bison Exc.
Soil
Ph
oto
syn
thet
ic F
rin
ge Octopus Spring
-25
-20
-15
-10
-5
0
50 60 70 80 90 100Temperature (C)
D13 C
fro
m D
IC (
‰)
Ph
oto
syn
thet
ic F
rin
ge Spent Kleenex
Spring
Bison vs. Spent Kleenex
Similar chemistry, similar isotopes
Bison vs. Octopus Spring
Similar chemistry, different isotopes
(J. Havig, in prep.)
Thank YouThank You
• Jeff HavigJeff Havig
• Dr. Ariel AnbarDr. Ariel Anbar
• Stephen RomanielloStephen Romaniello
• Dr. Achim HerrmannDr. Achim Herrmann
• Natasha ZolotovaNatasha Zolotova
• Dr. Everett ShockDr. Everett Shock
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