c. dna replication check your learning

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DNA Synthesis (Replication)

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DNA Structure and Function

Which method of replication would result in a DNA strand that contained fragments from both the parent strand and newly-synthesized portions?

A) Interspersed replication

B) Conservative replication

C) Semi-conservative replication

D) Dispersive replication

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DNA Structure and Function

Which method of replication would result in a DNA strand that contained fragments from both the parent strand and newly-synthesized portions?

A) Interspersed replication

B) Conservative replication

C) Semi-conservative replication

D) Dispersive replication

What is meant by the term “semi-conservative replication”?  

a) Replication requires a primer.  b) A single strand of DNA is synthesized complementary to a template strand, thus resulting in a double-stranded molecule where one strand is the original template, and the other is newly synthesized, complementary DNA.  c) The double-stranded DNA molecule includes strands that contain mixtures of template (original) and new nucleotides.  d) First the leading strand is synthesized, then the lagging strand.

What is meant by the term “semi-conservative replication”?  

a) Replication requires a primer.  b) A single strand of DNA is synthesized complementary to a template strand, thus resulting in a double-stranded molecule where one strand is the original template, and the other is newly synthesized, complementary DNA.  c) The double-stranded DNA molecule includes strands that contain mixtures of template (original) and new nucleotides.  d) First the leading strand is synthesized, then the lagging strand.

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DNA Structure and Function

Which key factors were utilized by Meselson and Stahl to show DNA replication followed a semi-conservative method?

A) Radiolabeled phosphate groups

B) Radiolabeled deoxyribose

C) Phosphorus isotopes

D) Nitrogen isotopes

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DNA Structure and Function

Which key factors were utilized by Meselson and Stahl to show DNA replication followed a semi-conservative method?

A) Radiolabeled phosphate groups

B) Radiolabeled deoxyribose

C) Phosphorus isotopes

D) Nitrogen isotopes

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DNA Structure and Function

Which is the main enzyme that adds new deoxyribonucleotides during DNA replication in prokaryotes?

A) DNA polymerase II

B) DNA polymerase I

C) DNA polymerase III

D) All of these.

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DNA Structure and Function

Which is the main enzyme that adds new deoxyribonucleotides during DNA replication in prokaryotes?

A) DNA polymerase II

B) DNA polymerase I

C) DNA polymerase III

D) All of these.

DNA Structure and Function

Which is the enzyme that travels ahead of the replication form, relaxing the double helix and making it possible for helicase to do it’s work?

A) Primase

B) Topoisomerase

C) DNA polymerase III

D) All of these.

DNA Structure and Function

Which is the enzyme that travels ahead of the replication form, relaxing the double helix and making it possible for helicase to do it’s work?

A) Primase

B) Topoisomerase

C) DNA polymerase III

D) All of these.

DNA Structure and Function

Which is the enzyme that synthesizes short sequences of RNA, used to allow DNA polymerase III to do it’s work?

A) Primase

B) Topoisomerase

C) DNA polymerase I

D) Helicase.

DNA Structure and Function

Which is the enzyme that synthesizes short sequences of RNA, used to allow DNA polymerase III to do it’s work?

A) Primase

B) Topoisomerase

C) DNA polymerase I

D) Helicase.

DNA Structure and Function

What proteins are necessary for maintaining the replication fork? (in other words, for keeping it open)

A) Primase

B) Topoisomerase

C) Ligase

D) Single strand binding proteins

DNA Structure and Function

What proteins are necessary for maintaining the replication fork? (in other words, for keeping it open)

A) Primase

B) Topoisomerase

C) Ligase

D) Single strand binding proteins

DNA Structure and Function

What enzyme is necessary for sealing the gaps at the ends of Okazaki fragments (in other words, catalyzing the formation of phosphodiester linkages in those particular areas)?

A) Primase

B) Topoisomerase

C) Ligase

D) Single strand binding proteins

DNA Structure and Function

What enzyme is necessary for sealing the gaps at the ends of Okazaki fragments (in other words, catalyzing the formation of phosphodiester linkages in those particular areas)?

A) Primase

B) Topoisomerase

C) Ligase

D) Single strand binding proteins

DNA Structure and Function

Label the leading and the lagging strands in the image below:

5’

3’

3’5’

DNA Structure and Function

Label the leading and the lagging strands in the image below:

5’

3’

3’5’

Leading strand

Lagging strand

DNA Structure and Function

Where will Okazaki fragments be found?

5’

3’

3’5’

Leading strand

Lagging strand

DNA Structure and Function

Where will Okazaki fragments be found?

5’

3’

3’5’

Leading strand

Lagging strand

DNA Structure and Function

In which direction will the overall synthesis proceed?

5’

3’

3’5’

Leading strand

Lagging strand

DNA Structure and Function

In which direction will the overall synthesis proceed?

5’

3’

3’5’

Leading strand

Lagging strand

DNA Structure and Function

What is an “origin”?

A) A protein that begins DNA synthesis.

B) A sequence of DNA that attracts proteins such as helicase.

C) A sequence of DNA that is the location where synthesis begins.

D) Both B and C.

DNA Structure and Function

What is an “origin”?

A) A protein that begins DNA synthesis.

B) A sequence of DNA that attracts proteins such as helicase.

C) A sequence of DNA that is the location where synthesis begins.

D) Both B and C.

DNA Structure and Function

DNA synthesis in eukaryotes is very similar to prokaryotes but there are some differences. What are they?

A) Because eukaryotic chromosomes are linear they have telomeres at the end. .

B) Eukaryotic chromosomes are much bigger, so you need many origins of replication rather than one single one per chromosome.

C) Eukaryotic chromosomes aren’t replicated in a semi-conservative manner.

D) Both A and B. .

DNA Structure and Function

DNA synthesis in eukaryotes is very similar to prokaryotes but there are some differences. What are they?

A) Because eukaryotic chromosomes are linear they have telomeres at the end. .

B) Eukaryotic chromosomes are much bigger, so you need many origins of replication rather than one single one per chromosome.

C) Eukaryotic chromosomes aren’t replicated in a semi-conservative manner.

D) Both A and B. .