bellwork (8 minutes only)
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Bellwork(8 minutes only)1. What makes
this compound microscope different from the dissection microscopes we’ve used?
2. Copy the labels onto your handout
3. Organize 3 ring binder (all in order) & review for test
NEW SEATS
BEFORE THE TEST- REVISIT THE ESSENTIAL QUESTION FOR ECOLOGYEssential Question
How do the interactions of living things and the environment affect our world?_______(old answer)__________ ____________________________________________________________________________________
NEW & IMPROVED ANSWER: ____________________________________________________________________________________________________________________________________________
ECOLOGY UNIT BINDER CHECK
Pull out the ecology Divider
+2pts each assignment present
Flip through notebook & score as Ms. D puts assignments on board
Circle score at top (24 pts possible). Pass forward.
Ecology Unit1. Notes
2. Labs
3. Assignments
4. Tests
-What is Life Notetaker-Energy Flow Notes-Trophic Levels & Rule of 10 Notes-Biome Poster Notes/Research Notes-Aquatic Macroinvertebrates & Water Quality-Population Notes
-Cricket Mark & Recapture Lab-Aquatic Macroinvertebrates Lab-Jackrabbits & Coyotes Lab
-What Is Life Quiz
-Critter Outline-Overpopulation Article
MICROSCOPE CARE & SAFETY
Take only from appropriate cabinet Always carry with 2 hands (base & arm) ONLY use lens paper to clean Do NOT force knobs Keep away from table edge &
keep objects clear of cords Store covered & w/ stage down
aka “put it in park”
1. Place slide on Microscope2. Use Stage Clips 3. Click Nosepiece to the lowest
(shortest) objective4. Look into Eyepiece5. Turn Coarse Adjustment (big knob) to focus;
move slide if nessary6. Click to medium objective7. Use Coarse & Fine Adjustments (big & little
knobs) to focus
8. Click the nosepiece to the high power (longest) objective
9. Do NOT use the Coarse Focus Knob anymore
10. Use ONLY the Fine Focus Knob (little knob) to focus on high power
What can you find on your slide?
AFTER YOU FINISH THE TEST, HEAD TO YOUR NEW LAB TABLE AND PRACTICE FOCUSING ON THE SLIDES!
Bellwork1. How can you tell the difference between low,
medium, & high power objectives?
2. Summarize the steps to focusing a microscope in 3-4 sentences (look @ your microscope notes for assistance)
3. How do you put a microscope into PARK?
4. What safety rule do you need to remember when using HIGH power?
**When finished, study your microscope parts diagram, short scratch paper quiz today!
6. Which power objective must you focus in FIRST?7. What is the ONLY knob you should use when
focusing on high power?8. To put the microscope in park @ the end, you must
cover it and put the stage all the way __________.
For 1-5, name the part being pointed to in the picture
Microscope Quiz
6. Which power objective must you focus in FIRST? LOW
7. What is the ONLY knob you should use when focusing on high power? FINE FOCUS
8. To put the microscope in park @ the end, you must cover it and put the stage all the way DOWN
NEW UNIT- NEW DIVIDER
1. Notes
2. Labs
3. Assignments
4. Tests
-Microscope Notes
Cells Unit Essential Question
Why are cells called the “basic unit of life”?
___________________________________________________________________________________________________________________
WHAT MAKES A GOOD MICROSCOPE DRAWING?- ON BACK OF MICROSCOPE NOTES Title (what is it, what power lens) Size (petri dish) Detail (Slow down! Count! Again!) Observations (complete sentences) Labels (use ID guides & vocab)
Plant cell (Eukaryote)
Observations•The algae is light green inside•The cells are all about the same size rectangles•There are 16 strands of algae in view
Green algaeRhizoclonium
U of A pond water, high power
MICROSCOPE INTRO LAB (LAB BOOK)Question: What do cells look like under a
microscope?
Hypothesis: If…then…as measured by….
Procedures: 1. On HIGH power, draw 2+ QUALITY
microscope drawings.
Data (drawings):
SCORE ONE OF YOUR DRAWINGS (10 PTS POSSIBLE)
Title- 2pts (must describe sample AND power)Size- 1pt (at least as big as a medium petri dish)Detail- 3pts (3=very detailed, 2=some detail, 1=scribbles)
Observations- 3pts (3=two+ observations in complete sentences; 2=two+ observations, but incomplete sentences; 1=only on observation)
Labels- (1pt) at least one label on the drawing
ANNOUNCEMENTS Turn in critter projectPut new assignment & rubric on LEFT side
pocket (new assignment side)
New Critter AssignmentHabitat Card
& Graded Critter
Assignments
The saw-toothed slitherfish has several adaptations that help it survive in a Coral Reef. It’s dagger-like teeth are its best hunting weapon, because they allow it to puncture the
Namep.__
RUB
RIC
CLOSURE1. On a scale of 1 (best) to 3 (worst), where do you
rate your ability to focus on high power?
2. Explain why you gave yourself that rating.
3. Answer the question below based on your rating:
If you gave yourself a 1 what specific things can you do next class period to be a leader and assist students who need more help?
If you gave yourself a 2 or a 3, what specific things will you try doing next class period to gain confidence and practice?
BELLWORK1. Look back at yesterday’s
hypothesis did the cells you saw yesterday look like what you expected them to? Explain.
2. What is required for a microscope drawing? (list ALL parts)
3. Set up your lab notebook for today like picture on the right
Prokaryote vs Eukaryote LabBackground Info:
Prokaryote = bacteria cells Eukaryote = plant & animal cellsQuestion: What differences can you see between prokaryotic cells and eukaryotic cells under the microscope?
ANOUNCEMENTS- SCORE & PASS UP BW
Science Fair ProposalApproved: You earned half of your points.
Staple a lined piece of paper to back addressing ALL comments to earn the rest.
Not approved: You earned 0 pts and must staple lined sheet of paper with new proposal to earn any points back. DUE MONDAY!
In computer lab ROOM 38 (DIFFERENT!) next Tuesday to do experimental design in rough draft packet
ANNOUNCEMENTS Turn in critter project (Bio 1-2 turn in
today) Pre-AP- attach late pass to turn in
todayPut new assignment & rubric on LEFT side
pocket (new assignment side)
New Critter AssignmentHabitat Card
& Graded Critter
Assignments
The saw-toothed slitherfish has several adaptations that help it survive in a Coral Reef. It’s dagger-like teeth are its best hunting weapon, because they allow it to puncture the
Namep.__
RUB
RIC
CELLS COME IN A WIDE VARIETY OF SHAPES AND SIZES
Stained MAMMAL TISSUE slides typically show the nucleus as a small,
dark spot did you see these yesterday?
Prokaryote= bacteria cells
Eukaryote= plant & animal cells
PROKARYOTE VS EUKARYOTE LABBACKGROUND INFO:
PROKARYOTE VS EUKARYOTE LAB
Question: What differences can you see between prokaryotic and eukaryotic cells under the microscope?
Hypothesis:IfThenAs Measured By
Make a prediction about size, shape, or other characteristic you can SEE
PROCEDURES1. View prepared slide of prokaryotes
(1 HIGH POWER drawing of CELLS required)
2. Prepare wet mount of pond water & view eukaryotic cells (1 HIGH POWER drawing of CELLS required)
-Label slide w/ masking tape-Put 1-2 drops pond water on clean slide-Put cover slip on top
PROCEDURES3. Clean-up
-Rinse slides & cover slips @ BOTTOM
of sink (in case you drop)-GENTLY dry slides w/ paper towel
-Sanitize table & dry-Wash hands
JOBS ROLES- PARTNERS Microscope Expert
Takes microscope ½ sheet ONLY fine focus knob used on high power Keeps microscope away from edge
Slides Safety Expert Makes sure everyone is being PAYING
ATTENTION while preparing & cleaning slides Makes sure slides are labeled Alerts Ms. D immediately of any breaks
PARTIAL CONCLUSION: 3-4 SENTENCES
What did you learn about prokaryotes vs eukaryotes? Explain.
PARTIAL CONCLUSION: 3-4 SENTENCES
Summarize your hypothesis and say whether it was supported or rejected?
What observations supported or rejected your hypothesis? (give details)
What did you learn about prokaryotes vs eukaryotes? Explain.
BELLWORK 1. Sketch a prokaryotic cell & a eukaryotic cell.
2. What were the major differences you saw between the two cell types?
3. Finish your 3-4 sentence conclusion for yesterday’s lab- IN YOUR NOTEBOOK:
-Was your hypothesis supported or refuted?-What observations supported or refuted your hypothesis (explain)-What did you learn about prokaryotes & eukaryotes?
Cells are divided into 2 major categories based on how the DNA is stored:
Prokaryotes or Eukaryotes
Remember DNA? It’s
what codes for your
GENES and makes you who you
are!
PROKARYOTES (PRO= “BEFORE”; KARYON = “NUCLEUS”) NO nucleus to store DNA, it floats
coiled in the middle of the cell Very small and simple structure Example: Bacteria
Draw and label the DNA on
your diagramDNA
EUKARYOTES(EU = “TRUE” ; KARYON = “NUCLEUS”) DNA stored in nucleus
surrounded by a protective membrane
Larger size, complex structure Examples: plants and animals
Draw and label the DNA on
your diagram
DNA
CELL SIZE VISUALIZATION
http://learn.genetics.utah.edu/content/begin/cells/scale/
WHAT ARE THE MOST IMPORTANT ORGANS IN YOUR BODY? WHAT IS THE
FUNCTION OF EACH ONE?
Talk to your partner & be
ready to share
ORGANELLES Organelles are structures that work like
miniature organs within the cell to perform specific functions (jobs). ONLY found in eukaryotes.
1. NUCLEUS Contains the genetic material (DNA/genes) Surrounded & protected by a nuclear
membrane Directs all the activities of the cell. Analogy = the “brain” or “jefe” of the cell.
LABEL THE NUCLEUS & NUCLEAR MEMBRANE ON YOUR EUKARYOTE
NUCLEUS
NUCLEARMEMBRANE
Do prokaryotes
have a nucleus?
NO!!!!
2. MITOCHONDRIA Convert sugars into ATP energy
(ATP is the chemical used to power the cell)
They have their own DNA! They probably came from a eukaryote engulfing a PROKARYOTE!
Analogy = “Powerhouse of the cell”
DRAW & LABEL A MITOCHONDRION IN YOUR EUKARYOTE
MITOCHONDRIA
OTHER CELL PARTS (NOT ORGANELLES)
3. CYTOPLASM A gel-like substance that fills the cell
(mostly made of water) Materials in the cell are suspended in
the cytoplasm Analogy = “the glue that holds
everything in place”
LABEL THE CYTOPLASM ON YOUR PROKARYOTE & EUKARYOTE
CytoplasmCytoplasm
4. CELL MEMBRANE Separates & protects the cell from
the outside environment It is SEMI-PERMEABLE (let’s some
things in, keeps other things out) Analogy = “It’s the bouncer of the
cell”
LABEL THE CELL MEMBRANE ON YOUR PROKARYOTE & EUKARYOTE
Cell MembraneCell
Membrane
5. CELL WALL A tough, rigid, outer cell layer (outside the
cell membrane) Provides extra strength; helps cells keep
their shape Found in plants & bacteria, NOT in animal
cells Analogy = “Spanx of the cell- keeps the
shape”
LABEL THE CELL WALL ON YOUR PROKARYOTE
Cell Wall (outer most layer)
If we’re not labeling a cell
wall on our eukaryote…does
that make it a plant or animal
cell?ANIMAL!!!!
WHAT OTHER CELL PARTS & ORGANELLES ARE THERE?
Use the textbook Chp. 3.1-3.3 to fill out your cell parts worksheet
CLOSURE- ON NOTETAKER Make a Venn Diagram for Prokaryotes vs
Eukaryotes and decide where in the Venn Diagram to write each of the following terms:
Prokaryotes
Eukaryotes
Nucleus Cell Wall Cell MembraneMitochondria Cytoplasm AnimalBacteria PlantsOrganelles
BELLWORK1. What is 1 major difference between eukaryotes and
prokaryotes?
2. Look at your notes from yesterday- what 2 ORGANELLES did you learn about? What was the analogy for each one?
3. Which organelle would you expect a cell to have only ONE? Which organelle would there be many of in the same cell?
4. Title the next page in your notebook “Eukaryotic Cells Lab: Plant vs Animal”
5. Copy the Question: “If plant and animal cells are both eukaryotic, how do they compare under the microscope?”
6. Write an If…then…as measured by…. Hypothesis. Be ready to share!
LABELING SLIDES Always label the slides you prepare!
Use masking tape & penPut label on TOP of slide Include DATE and DESCRIPTION
10-1-12Jessica’s cheek cells
PROCEDURESA. Prepare a slide of your cheek cells
1. Put 1 drop methylene blue on labeled slide 2. GENTLY scrape inside of cheek w/ toothpick 5-6 times3. Swirl toothpick into methylene blue on slide4. Place cover slip on top5. Clean upLeave slide & cover slip in bleach solution 1-2 mins. before rinsing
& drying
B. View 2 different prepared slides of mammal tissue
C. Prepare a wet mount of an onion skin6. Peel off a THIN layer of onion skin7. Stretch the skin across slide8. Put 1 drop iodine stain on top of onion skin9. Place a cover slip on top
D. Prepare a wet mount of an elodea leaf10. Put one wet elodea leaf onto slide11. Place a cover slip on top
E. View 1 prepared plant slide (come get from Ms. D)
JOBS ROLES- PARTNERS Microscope Expert
Takes microscope ½ sheet ONLY fine focus knob used on high power Keeps microscope away from edge
Slides Safety Expert Makes sure everyone is being PAYING
ATTENTION while preparing & cleaning slides Makes sure slides are labeled Alerts Ms. D immediately of any breaks
DATA- SET UP W/ PETRI DISHES
1 HIGH power drawings of your CHEEK cells
2 HIGH power drawings of a mammal tissue slides
1 HIGH power drawing of onion skin cells
1 HIGH power drawing of elodea leaf cells
1 HIGH power drawing of a prepared plant slide
CLOSURE- OUT LOUD W/ GROUP Which organelle(s) and cell part(s) did
you see in your cheek cells?
BELLWORK1. What is due TOMORROW? (look @ science
fair due dates…)
2. Which of the 6 drawings did you complete yesterday?
3. What kinds of labels did you add to your drawing(s)?
4. What kind of QUANTITATIVE observations did you record?
*****When finished, skim through the handout to learn about an upcoming assignment
CELL ANALOGY BOOK ASSIGNMENTDue Mon. October 15th. Turn in early for ex.
credit!
1. 3 pages folded in ½ stapled together
2. Must do a EUKARYOTE. -Pick either PLANT or ANIMAL cell. Circle on sheet!
3. Include at least 10 cell parts (look at the comparison chart in notetaker for possibilities)
HOW THE BOOK WORKS
4. Compare your cell to something that has many parts. This is your title page.
-Example: A cell is like a school
5. Use the jobs of the cell parts to figure out how to relate them to the analogy
-Example: The nucleus directs all the activities of the cell…..who directs all the activities of the school?
-The principal!
Draw a picture & explain the analogy for each cell part. This goes on each page.
A cell is like a schoolby Ms. D
The principal is like the NUCLEUS because he
directs the activities of the school, just like the nucleus directs the activities of a cell.
PROCEDURES- GO FINISH!A. Prepare a slide of your cheek cells
1. Put 1 drop methylene blue on labeled slide 2. GENTLY scrape inside of cheek w/ toothpick 5-6 times3. Swirl toothpick into methylene blue on slide4. Place cover slip on top5. Clean upLeave slide & cover slip in bleach solution 1-2 mins. before rinsing
& drying
B. View 2 different prepared slides of mammal tissue
C. Prepare a wet mount of an onion skin6. Peel off a THIN layer of onion skin7. Stretch the skin across slide8. Put 1 drop iodine stain on top of onion skin9. Place a cover slip on top
D. Prepare a wet mount of an elodea leaf10. Put one wet elodea leaf onto slide11. Place a cover slip on top
E. View 1 prepared plant slide (come get from Ms. D)
AT YOUR DESK… Decide what you will compare your cell
to (what’s the title of your book?)
Start writing down what you could compare the nucleus, mitochondria, cell membrane & cytoplasm to….
PLANT VS ANIMAL CELLS Remember, eukaryotic cells are very
complex, and this category includes both plant and animal cells.
They have many cell parts and organelles, but there are some differences between plant and animal cells.
CELL PART COMPARISON CHARTCharacteristic/Cell Part ANIMAL cell PLANT cell
Overall Shape of Cell Round (irregular shape) Rectangular (fixed shape)
Cell wall - +Plasma (Cell) Membrane + +Cytoplasm + +Mitochondria + +Nucleus + +Smooth Endoplasmic Reticulum + +Rough Endoplasmic Reticulum + +Golgi Apparatus / Golgi Body + +Ribosomes + +Lysosomes + Usually -Microtubules / Microfilaments + +Centrioles + Usually -Flagella Sometimes Sometimes
Cilia + Rare
Chloroplast - +Vacuole + (small) + (VERY large, centrally located)
PLANT CELLS Plastids are special cell parts that are
found only in plants.
What differences did
you see looking at the
plant cells?
6. CHLOROPLASTS Use energy from the sun to make sugars
(food) for the cell through photosynthesis! Contain a green pigment called chlorophyll Analogy = the CHEF of the cell! (makes the
food)
7. VACUOLE A large central fluid-filled sac that regulates
water content & keeps cells “full” & rigid HUGE inside plants, can take up 90% of the
cell volume! Analogy = the WATER TOWER of the cell!
CLOSURE Write a 3 sentence conclusion for the lab
Was your hypothesis supported or rejected? What observations supported or rejected your
hypothesis? What did you learn about plant vs animal cells (use
vocab)?
Update your table of contents for the Prokaryote vs Eukaryote Lab AND the Plant vs Animal Cell Lab
When you are finished, begin labeling your plant vs animal cell diagram on the back of your note taker
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