basics of spectrophotometry
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Basic of SpectrophotometrySMK Negeri 13 Bandung
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Dispersion of f Polichromatic RadiationRadiation sourceVisible Ray Spectrum
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Color Complementer PropertyarnaAdsorbed coor is complement of reflected colorPurple-dark blueYellowRedHijauOrangeBlue
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Absorption of visible ray and color
AdsorbedRadiationReflected Color (Eye cacthed)ColorWavelength (nm)Purple-dark blue400-435YellowBlue450-480OrangeGreen500-560RedYellow585-590Purple-dark blueOrange590-605BlueRed607-770Green
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REM wave
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REM Spectrum
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REM SpectrumExcitationSpectroscopic Method:
Electronic Molecule NucleusEnergy Vibration ResonanceUV-Vis IR NMR
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Interaction of REM - MatterRadiation inDiffusion
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Absorption dan EmissionElectromagnetic Emission, Radition Spectroscopic AbsorptionElectromagnetic Emission, Radition Spectroscopic EmissionGround stateExcited state
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Absorption dan EmissionE= Energi (J)E= Energi Difference (J)h= Planck Constant (6,626 . 10-34 Js)c= Ligth Velocity 3 . 108 m/s= Frequence (Hz)
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Absorption
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The Lambert- Beers LawConcentration in mole/ literCuvette thickness in cmAbsorbance (No dimension) Molar Absorptivity In liter / mole cm
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UV/VIS Spectroscopy Basic theory (Color Spectrum , Light Absorption and Lambert-Beers Law)InstrumentationApplication
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Visible Spectrum High Freq.Low Freq. Wave length (nm)
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Molecular OrbitalBonding MO Anti bonding MO Non bonding MO Energi equivalent to REM at 200 800 nm
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Electronic TransitionAnti Bonding MO Bonding MO
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Band Spectrum
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Example of UV Spectrum
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Prequisite for UV/Vis Absorption by substance Chromophor existence : Double bonding system(1) aromatic (delocalized double bond)(2) conjugated double bond with functional group with (+)- and (-)-M-Effect(3) triple bond
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Chromophor
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Chromophor
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Carbonyl substance
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Aucsochrom
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II. Instrumentation:Block Diagram of UV/Vis spectrofotometerRadiation sourceSlotSlotDetectorPolichromatic RadiationMonochromatic RadiationCuveteSlotABA : sampleB : Reference
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Double BeamUV/Vis Spectrofotometer
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Monochromator:PrismLattice
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DADPMTDetector
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Types of cuvette
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SEE YOU AGAINThank You
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