ascos planned aerosol instrumentation aboard oden douglas orsini jost heintzenberg leibniz institute...

ASCOS Planned Aerosol Instrumentation Aboard Oden

Douglas Orsini

Jost HeintzenbergLeibniz Institute for Tropospheric Research

Leipzig, Germany

Motivation

To contribute to measured physical and chemical properties of the marine aerosol ( especially Dp < 1 um) and to target biological particles

Three instruments:

1. DMPS

1. VTDMA

1. Water Cyclone

DMPS

Differential Mobility Particle Sizer

Goal: To measure the aerosol size distribution

Particle size range: 3 nm < Dp < 800 nm

Sample Flow Rate: 1 lpm

Measurement Rate: Semi-continuous every 10 min.

VTDMA

Volatility Tandem Differential Mobility Particle Analyzer

Goal: To infer composition and mixing state based on reference volatility measurements

1. Select particle size (30 nm < Dp < 300 nm; 1 lpm)

2. Heat the selected aerosol size ( 3 temperatures possible )

3. Re-measure the aerosol size

VTDMA

Volatility Tandem Differential Mobility Particle Sizer

Some reference temperatures:

• ~ 150 - 200 oC ( H2SO4, (NH4)HSO4, (NH4)2SO4 )

• ~ 300 oC ( volatile OC )

• ~ 600 - 800 oC ( less volatile OC + NaCl )

• ~ ? ( viruses, bacteria, cell material )

- fine marine aerosols exist capable of surviving at 300oC (Clarke, 1999, Clarke et al., 2003)

- little data on volatility of viruses and bacteria as ambient particles

To Be Determined: selected sizes + heating temperatures

Time permitting: prelimin. tests of bubbled microlayer water to determine temperatures.

Water Cyclone

Collects bulk aerosol into liquid (insoluble + soluble)

Sample flow rate: 17 lpm continuous

Liquid sample flow: 24 hours: 100 - 1000 ml of sample liquid for analysis

Consumables: 0.02 ultrapure water : 5 liters / day

Liquid Sample Analysis

Water Cyclone

On-Board

Biological Enumeration

Storage for Post Analysis

Chemical and Biological

Water Cyclone

On-Board Biological Enumeration

Ship-board analysis of aerosol sample: (pending fluorescence microscope)

1. Concentrate sample liquid on .02 filter substrate (start with 24 hr. sample)

2. Stain with Sybr-Green/Gold which binds to nucleic acid

3. Enumerate biological particles (based on known protocol) with microscope

Post Analysis

Water Cyclone

microlayer liquid & bubbled microlayer

aerosol collection into liquid

Coordinate similar sample preparation and storage protocols

e.g.: TOC

Basic ions

Amino acids

EPS gels

flow cytometry

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What is possible for consistent analysis ?

microlayer liquid & bubbled microlayer

aerosol collection into liquid

fogwateraerosol

impactors

Coordinate parallel sample storage and protocols

An upper Dp size cut-off ?

e.g. :

Water Cyclone

PM10 aerosol

impactor

Dp < 100 nmcollection into liquid

Counting Protocol for Marine Samples