activate: how human milk and breast/chestfeeding activates...

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Activate:HowHumanMilkandBreast/ChestfeedingActivatesOurGenesThroughEpigeneticsByLaurelWilson,IBCLC,RLC,BSc,CLE,CCCE,CLDwww.facebook.com/motherjourneylaurelwilsonwww.instagram.com/motherjourneylaurelwilsonwww.motherjourney.comSubscribetoresearchnewsletterbytextingBreastmilkto66866Recentresearchonepigenetics,literallymeaningabovethegene,hasledmedicalprofessionalstoqueryabouthowtheenvironmentimpactsthedevelopingbabybothinuteroandthroughoutitslifetime.Thegenomeisthegeneticinformationinheritedfromone'sparents,buttheepigenomeiswhatdeciphersthegenomeforeachcellthroughoutthebody.Thisdecipheringprocessisimpactedbyanindividual’sinternalandexternalenvironment.Theexternalenvironmentcanincludenutrition,chemicals,toxins,etc.Theinternalenvironmentwouldincludeneuropeptides(emotionalmolecules)andstresshormones.TheenvironmentchangestheproteinsinthebodythathelptheepigenometranslateDNA.Thisfindinghasincreasedawarenessoftheimportanceofnutritionontheepigenome.Studiesnowarefindingthatthechangesintheepigenomecaninfluencenotonlythatindividualbutcanbepassedalongtofutureprogeny,sometimesmultiplegenerationsout.Thefirstnutritionforahumanoutsidethewombishumanmilk,andthusitsepigeneticimpactisparticularlyexpansive.Newresearchhasexpandedthefieldofepigeneticstoincludehumanmilkandhowitpotentiallychangestheepigenometoaffectthelifelonghealthofababy.LearningOutcomes:• Definethetermsepigenomeandgenome.• Listonewaythathumanmilkcanhaveapositiveimpactonthe

epigenomeoftheinfant.• Whatarethethreemainareasofinfluenceontheinfantduringthe

criticalthousanddays.

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TopTakeaways:The7Generations

¡ Everymothercontainsherdaughterinherselfandeverydaughterhermother.Everywomanextendsbackwardsintohermotherandforwardintoherdaughter.~CarlJung

¡ 14generationsinanimalstudiesThreegenerationsofexposureatonce “Threegenerationsatonceareexposedtothesameenvironmentalconditions(diet,toxins,hormones,etc.).Inordertoprovideaconvincingcaseforepigeneticinheritance,anepigeneticchangemustbeobservedinthe4thgeneration.”https://learn.genetics.utah.edu/content/epigenetics/inheritance/Only1-2%ofdiseasecomesdirectlyfromgeneticorigin.Ourgenesdonotdirectourlivesbecauseourgenescannotturnthemselvesoffandon.Onlytheenvironmentcandothat!WhatisEpigenetics?• Literallymeansabovethegene.• Howourenvironmentinfluencesgeneticexpression.• Genome=DNAplusEpigenome=Phenotype

FlipSwitch• Environmentalandchemicalexposures• Nutrition• Emotions,thoughts,beliefs• Stress

HumanMilk’sEpigeneticInfluence• Lactoferrin–ImmuneProtection• Prostaglandin–ObesityDisorders• LCPUFAn-3andCholesterol–Non-AlcoholicFattyLiverDisease

andHighBloodCholesterol• Oligosaccharides–NEC,ImmuneDisorders,Obesity

HumanMilkandMicrobiome• ArtificialmilkfedinfantshavecompletelydifferentpH.ChangespH

(fromanacidicbaseof5.1-5.4tomorealkalineof5.9-7.3whichallowsputrefactivebacteria)

• Changethefermentationprocessandmethylgroupsavailabletobody

• MicrobiomeDifferences

Canani,2011

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EpigeneticInfluences:• Methylationinfluencedbymaternalnutritionandexposure• ThriftyPhenotype(Hales,1992)• Infantbirthweight,gestationalageatbirth,andneurobehavioral

measures.(Marsit,2016)• PossiblethatplacentamRNAchangessetupdiseaseriskforlater

inlifeTheCriticalThousandDays

Mischka,2013 Humanmilkincreasesbifidobacterial,increasefolatewhichpromotesmethylationwhichdecreasesgenetranscription InfantformulaincreasesfirmicutewhichincreasesbutyratewhichpromoteshistoneacetylationwhichincreasesgenetranscriptionButyrateisashortchainfattyacid,contributingtofataccumulationhyper-acetylationMaybeonemechanismforobesityriskCafeteriaDietsDuringLactation

• CD-Highfat,highsugar,lowprotein• Duringlactationinrats(Pomar,2017)

o Lowerbodyweight,lowerleanmass,highfataccumulationo Hyperleptinemiao Thinoutside-fat-insidebodytype

CafeteriaDietsDuringLactation• CD-Highfat,highsugar,lowprotein(Speight,2017)

§ Reducedanxiety§ Locomotorincreased§ Altersmethylationtags

Kumar,2106Fattyacidprofilesandmicrobiotainhumanmilkdifferbasedontheregiontheparentlivesinandthetypeofbirththeyhad(vaginalvs.cesarean)Kosakaetal.

¡ HighlevelsofmiRNAinbreastmilkinfirstsixmonthsoflactation¡ Approximately1.3×107copies/liter/dayofmiR-181a¡ Onlyothertimehumanstransfergeneticinfooutsideof

reproduction

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Humanmilkfeedingallowsfortransferofgeneticmaterialbetweenhumans.• miRNAssurviveharshconditions

Prolongedroomtemp.AcidicconditionsRnasedigestionBoiling!!!!Pasteurization• Influenceisstrongestbeforeageof2

• Inadequacyofimmunesystemtorejectgeneticmaterial• Increasedplasticity• Increasedvulnerabilityofepigenomeduring

developmentalperiodMoore,2017Holdingyourbabycreatesepigeneticchange At4.5YearsLowContact=LessMatureCellsTheBehaviorofBreast/Chestfeeding• Poornurturing=Methylationoftheestrogenreceptor(ER)alpha

gene=futurepoormaternalbehavior.(Champagne)• Estrogengeneisimplicatedinoxytocin-keytoattachment,

maternalbehaviorandMOODstability!!!!Lactationchangespostpartumbrain,morethan700genesmaternalexpressedS.C.Gammieetal,2016Weinstock,2014PotentialBrainChangesinBabiesDuetoPrenatalStress• PrenatalStress

• ImpairsHPAAxis• DecreaseSocialInteraction• Anxiety• LearningImpairments

• PostpartumFostering• RepairHPAAxis• DecreaseAnxiety• NoChangeLearningDeficit• NoChangeinSocialization

EpigeneticInheritance¡ Humanresearch-influencebeyondthreegenerations–

HongerWinterwinter1944-spring1945¡ 30%nutrition¡ Earlypregnancyorlatepregnancy¡ Specificepigenetictagsfordiabetes

WhatisWeathering?“..earlyhealthdeteriorationasaconsequenceofthecumulativeimpactofrepeatedexperiencewithsocialoreconomicadversityandpoliticalmarginalization.”Geronimus,2006

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Marginalization,oppression,racism=earlyhealthdeterioration• Racism

• ChronicStress• DailyMicroaggressions• Poverty• LackofHCAccess• FoodInsecurity• PersonalTrauma

• WeatheringImpactingFeeding• Earlyreturntowork• Increasedformulafeeding• Safetyforbreastfeedinginpublic• LackofDonorMilkinNICUinHospital

Ultimatelyhumanmilkisimportant¡ ShareMilkSystems¡ ImprovingdonationstoDonorMilksites¡ Improvingaccesstohumanmilkforthoseinneed.¡ Wetnursing???¡

Questions,handouts,resources:¡ info@motherjourney.com¡ motherjourney.com¡ Facebook,Instagram

¡ @motherjourneylaurelwilsonQuestions?info@motherjourney.comFacebook.com/motherjourneylaurelwilsonTwitter.com/linfiniteeBibliography:Foraconstantlyupdatedversion,seewww.motherjourney.com

• Canani,Retal.Epigeneticmechanismselicitedbynutritioninearlylife.Nutritionresearchreviews,2011.24;198-205.

• Chung,etal.RoleofCompensatoryMammaryGrowthinEpigeneticControlofGeneExpression.FASEBJournal.Vol.19.2005

• Costandi,M.Microbesmanipulateyourmind.ScientificAmericanMind.2012.23(3)• DOHaD.InternationalSocietyfortheDevelopmentalOriginsofHealthandDisease.Available

athttp://www.mrc.soton.ac.uk/dohad/index.asp.Accessed14January2013.• DonerayH,OrbakZ,YildizL:Therelationshipbetweenbreastmilkleptinandneonatalweight

gain.ActaPaediatr.98(4),643–647(2009).• DosSantos,Camilaetal.AnEpigeneticMemoryofPregnancyintheMouseMammaryGland.

Cellreports.May7,2015.

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• Einstein,Francine.ChallengesofLinkingEarly-LifeConditionsandDiseaseSusceptibility.DiabetesMay201261:981-982;doi:10.2337/db12-0087

• Flannick,Jetal.AssessingthephenotypiceffectsinthegeneralpopulationofrarevariantsingenesforadominanatMendelianformofdiabetes.NatGenet.2013.45(11):1380-1385.

• Kosakaetal.:microRNAasanewimmune-regulatoryagentinbreastmilk.Silence,2010,1:7.• Kuzawa,C.W.andSweet,E.(2009),Epigeneticsandtheembodimentofrace:Developmental

originsofUSracialdisparitiesincardiovascularhealth.Am.J.Hum.Biol.,21:2–15.doi:10.1002/ajhb.20822

• Kuwaza,C.etal.Timescaleofhumanadaptation:theroleofepigeneticprocesses.Epigenomics,3(2),221-234,2011.

• KuzawaCW:Fetaloriginsofdevelopmentalplasticity:arefetalcuesreliablepredictorsoffuturenutritionalenvironments?Am.J.HumanBiol.17(1),5–21(2005).

• Lackey,K.A.,Williams,J.E.,Meehan,C.L.,Zachek,J.A.,Benda,E.D.,Price,W.J.,...&Mbugua,S.(2019).What’sNormal?MicrobiomesInHumanMilkAndInfantFecesAreRelatedToEachOtherButVaryGeographically:TheINSPIREStudy.FrontiersinNutrition,6,45.https://doi.org/10.3389/fnut.2019.00045

• Langley-Evans,SC.Nutritioninearlylifeandtheprogrammingofadultdisease:areview.JHumNutrDiet.2014Jan31.doi:10.1111/jhn.12212.

• Lee,YK.Hasthemicrobioticaplayedacriticalroleintheevolutionoftheadaptiveimmunesystem?Science.2010.330(6012)1768-1773.

• Mischke,MonaandTorstenPlöschAmJPhysiolRegulIntegrCompPhysiol2013;304:R1065-R1069

• NationalInstitutesofHealth.NationalHumanGenomeResearchInstitute.AlAbouttheHumanGenomeProject.AccessedJan2014.http://www.genome.gov/10001772

• Nilsson,Eetal.EnvironmentallyInducesTransgenerationalInheritanceofOvarianDisease.PLOSOne.May03,2012DOI:10.1371/journal.pone.0036129

• Obermann-Borst,Setal.DurationofbreastfeedingandgenderareassociatedwithmethylationoftheLeptingeneinveryyoungchildren.PediatrRes74:344-349;advanceonlinepublication,July24,2013;doi:10.1038/pr.2013.95

• Ozkanetal.:Milkkinshiphypothesisinlightofepigeneticknowledge.ClinicalEpigenetics2012,4-14.

• Park,Chung.Roleofcompensatorymammarygrowthinepigeneticcontrolofgeneexpression.FASEBJournal,2005.Vol19,no.12.

• RasmussenKM,HabichtJ-P:Maternalsupplementationdifferentiallyaffectsthemotherandnewborn.J.Nutr.140(2),402–406(2010).

• SavinoF,LiguoriSA,FissoreMF,OggeroR:Breastmilkhormonesandtheirprotectiveeffectonobesity.Int.J.Pediatr.Endocrinol.2009,327505(2009).

• Simmons,R.Epigeneticsandmaternalnutrition:naturev.nurture.ProcNutrSoc.Nov29:1-9,2010.

• Thayer,Zetal.Biologicalmemoriesofpastenvironments.Epigenetics6:7,798-803:July2011.• T.H.-T.Tan,etal.TheRoleofGeneticsandEnvironmentintheRiseofChildhoodFoodAllergy.

ClinicalandExperimentalAllergy.2012(42)20-29.• Tow,Jennifer.Healthemother,healthebaby:epigenetics,breastfeedingandthehuman

microbiome.BreastfeedingReview.2014.22(1):7-9.

Mayonlyprintwithpermission. www.motherjourney.com

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• Valles,Y.etal.Metagenomicsandthedevelopmentofthegutmicrobioticaininfants.ClinicalMicrobiologyandInfection.2012.Publishedonline.http://www.lcg.unam.mx/frontiers/files/frontiers/Valle%60s_etal_final.pdf

• Verduci,E.;Banderali,G.;Barberi,S.;Radaelli,G.;Lops,A.;Betti,F.;Riva,E.;Giovannini,M.EpigeneticEffectsofHumanBreastMilk.Nutrients2014,6,1711-1724.

• Waly,M.etal.PrenatalandPostnatalEpigeneticProgramming:ImplicationsforGI,Immune,andNeuronalFunctioninAutism.AutismResearchandTreatment.Volume2012.http://dx.doi.org/10.1155/2012/190930

• Waterland,RobertandJirtle,Randy.EarlyNutrition,EpigeneticChangesatTranspoonsandImprintedGenes,and20:63-68.2004.