1 chapter 5 the structure and function of macromolecules essential ideas: compounds of carbon,...
Post on 13-Dec-2015
216 Views
Preview:
TRANSCRIPT
1
Chapter 5
The Structure and Function of Macromolecules
Essential Ideas:
•Compounds of carbon, hydrogen and oxygen are used to supply and store energy.
•Proteins have a very wide range of functions in living organisms.
•The structure of DNA allows efficient storage of genetic information.
2
TOK
• There are conflicting views as to the harms and benefits of fats in diets. How do we decide between competing views?
2
3
TOK
• The story of the elucidation of the structures of DNA illustrates that cooperation and collaboration among scientists exist alongside competition between research groups. To waht extent is research in secret “anti-scientific”?
• What is the relationship between shared and personal knowledge in the natural world? 3
4
Macromolecules• There are many molecules that comprise
living organisms and many of them are quite large.
• As such, they are called macromolecules.
• There are 4 main macromolecules that comprise living organisms:– 1. Proteins.– 2. Lipids.– 3. Carbohydrates.– 4. Nucleic acids.
5
Macromolecules• Three of the four classes
(carbohydrates, protein, and nucleic acids) are comprised of polymers which are long molecules made up of the same or similar subunits covalently linked together.
• The subunits that are linked together are called monomers.
6
Macromolecules
• The synthesis and breakdown of macromolecules is governed by a series of condensation/dehydration or hydrolysis reactions.
7
Condensation Reactions
• In a condensation reaction (aka a dehydration reaction) monomers are added to a growing polymer and a water molecule is given off--hence the name dehydration reaction.
• These reactions require energy and the action is carried out by enzymes.
9
Hydrolysis• Hydrolysis is the process that adds a water
molecule to a polymer giving one of the monomers.
• This occurs during digestion and it makes the large molecules useable by our bodies by producing smaller subunits that our cells can uptake.
• The four main types of macromolecules can be broken down further and analyzed.
11
Carbohydrates• Carbohydrates are molecules
consisting of carbon, hydrogen, and oxygen.
• The three classifications are monosaccharides or simple sugars, disaccharides or double sugars, and polysaccharides which are 3 or more sugars linked together forming a sugar polymer.
12
Monosaccharides
• Monosaccharides generally have the formula CnH2nOn and depending on the location of the carbonyl group, the sugar is either an aldose or a keytone.
• When the carbonyl group is the last carbon on the chain, it is an aldose, when it is not, it is a keytone.
13
Monosaccharides
• Glucose C6H12O6 is an aldehyde that is a common monosaccharide used as energy by our body.
14
Monosaccharides
• Not only is it used as an energy energy source (as are other monosaccharides), but their carbon skeletons serve as building blocks for other major macromolecules within the organism--amino acids and fatty acids.
15
Disaccharides
• A disaccharide, such as sucrose, lactose, and maltose are joined together by a glycosidic linkage--a covalent bond between 2 monosaccharides by a dehydration reactions.
16
Disaccharides
• The fruit of a plant is often a great source of sucrose--glucose and fructose (the most common disaccharide) because as the plants form it during photosynthesis, this is where it gets stored.
17
Disaccharides• Other common disaccharides include
lactose and maltose.
• Lactose = milk sugar.
• Maltose = formed from the breakdown of starch by amylase.
http://en.wikipedia.org/wiki/Maltose#mediaviewer/File:Maltose2.svg
http://en.wikipedia.org/wiki/Lactose
18
Polysaccharides
• When monosaccharides are glycosidically linked together thousands of times, polysaccharides are formed.
• There are 2 main types of polysaccharides, structural and storage.
19
Structural or Storage?
• There are actually 2 forms of glucose, α and β, and the form is determined by the placement of a hydroxyl group.
20
Structural or Storage?• In α-glucose, the -OH group is below
the ring structure and in β-glucose, it is above the ring structure.
QuickTime™ and aGIF decompressor
are needed to see this picture.
21
Structural Polysaccharides• In structural
polysaccharides, strong materials are built to provide strength and support for the organism.
• A good example of this is cellulose.
22
Structural Polysaccharides
• Plant cell walls are comprised of cellulose which gives them strength. Some animals such as cows can digest cellulose, but humans can’t.
• For us, it is called “insoluble fiber” and is found in many fruits and vegetables.
23
Structural Polysaccharide
• Another important structural polysaccharide is chitin.
• Chitin makes up the hard exoskeleton of arthropods, and the cell walls of fungi.
24
Storage Polysaccharides
• Although the starch and cellulose molecules are comprised of glucose molecules, the different types of glucose give them vastly different uses.
• For example starch can be used by humans for food, cellulose can’t.
25
http://en.wikipedia.org/wiki/Amylose#mediaviewer/File:Amylose2.svg
26
Amylose and Amylopectin
• Starch is comprised of two components: amylose and amylopectin.– Amylose comprises 20-30% of the structure
and is more resistant to digestion.– Amylopectin makes up 70-80% of a starch
molecule and is more easily digested.
http://en.wikipedia.org/wiki/Amylose
http://en.wikipedia.org/wiki/Amylose#mediaviewer/File:Amylose2.svg
27
Storage Polysaccharides• There are two main
types of storage polysaccharides: That which is found in plants (starch), and that which is found in animals (glycogen).
• Glycogen is branched, and starch is helical.
28
Proteins• Proteins are another
class of macromolecules consisting of many monomers linked together by peptide bonds forming polymers.
• Enzymes are protein polymers that act as catalysts within cells.
29
Proteins• Polymers of amino acids are called
polypeptides and the same 20 amino acids form all polypeptides.
• Proteins consist of one or more of the polypeptides coiled into a specific conformation.
30
Proteins• Amino acids are
organic molecules containing a carboxyl group and an amino group.
• These are each bounded to a central carbon atom which also has an amino acid attached to it.
α carbon
Amino Group
Carboxyl Group
31
Proteins
• There are 2 groups that are linked together to form a peptide bond.
• A peptide bond is a dehydration reaction which links the amino group of one amino acid to the carboxyl group of a different amino acid yielding water in the process.
33
Proteins• Once amino acids are added to the
polypeptide, they begin to interact with the other amino acids on the growing polypeptide.
• The sequence then determines the 3D conformation the protein will take.
• The conformation of the protein determines how it will function.
34
Proteins
• An enzyme is an example and the conformation it takes determines how it functions when it interacts with a substrate.
• There are 4 different types of protein structure: 1°, 2°, 3°, and 4°.
35
Proteins--Primary Structure
• The primary structure is the specific sequence of amino acids.
36
Proteins--Primary Structure
• Proteins
37
Proteins--Primary Structure
• Primary Structure
• The order of the amino acids.
38
Proteins--Secondary Structure
• The secondary structure of the polypeptide is the coiled and/or folded patterns that emerges as the amino acids begin to interact with one another (H-bonds) not from the R-groups, but from the backbones of the amino acids.
39
Proteins--Secondary Structure
• Secondary Structure
• The folding configuration.
40
Proteins--Secondary Structure
• There are two main types of secondary protein structure, the α-helix and the β-pleated sheet.
• The α-helix is a delicate coil held together by H-bonds.
• The β-pleated sheet forms when 2 polypeptides are aligned side by side and hydrogen bond along their lengths.
41
Proteins--Tertiary Structure
• The tertiary structure of the protein is due to the interactions of the R-groups on the amino acids.
42
Proteins--Tertiary Structure
• Tertiary Structure
• R-group interaction.
QuickTime™ and a decompressor
are needed to see this picture.
44
Proteins--Quaternary Structure
• The quaternary structure of a protein occurs when 2 or more polypeptide chains interact with each other to form a large, functional protein.
45
Proteins--Quaternary Structure
• Quaternary Structure
• The final, finished protein.
QuickTime™ and a decompressor
are needed to see this picture.
47
Your Proteome• We know that proteins are coded for by our
genes.
• Each individual has a unique set of genes that code for each protein in your body.
• Thus, your proteome is unique to you. While you code for the same proteins as all other human beings, the minor differences we have is what make us all different.
48
Small Change = Big Problem
• Sickle cell anemia example: glutamic acid out, valine in, causes changes in quaternary structure.
49
Nucleic Acids• Nucleic acids store and
transmit information about a protein’s primary structure.
• There are 2 types of nucleic acids: RNA and DNA.
• DNA provides the necessary information for guiding its own replication.
• It also guides RNA synthesis and using RNA controls protein synthesis.
QuickTime™ and aGIF decompressor
are needed to see this picture.
50
Nucleic Acids• DNA is the information that controls the
cell. This information is used to create messenger RNA that interacts with the cells protein synthesizing machinery (ribosomes) to create the proteins that run the cell.
• DNA--> RNA --> Protein
52
Nucleic Acids• Nucleic acids are
polymers called polynucleotides which consist of monomers called nucleotides.
• The nucleotides are composed of a pentose (a 5-carbon sugar), a nitrogenous base, and a phosphate group.
53
Nucleic Acids• The nucleotide
monomers are built from sugar atoms (ribose and deoxyribose) attached to a nitrogenous base (C,T, U, A G). This forms a nucleoside which becomes a nucleotide once the phosphate group has been added.
54
Nucleic Acids• Nucleotides are linked together by covalent
(phosphodiester) bonds between a hydroxyl group on the 3’ carbon of one nucleotide and the phosphate on the 5’ carbon on the next.
• This bond starts the repeating sugar-phosphate bond. This gives rise to the 3’ and 5’ ends of the DNA molecule. The sequence of each of the bases in the polynucleotide is unique to each gene.
55
Nucleic Acids
• RNA consists of a single strand of nucleic acids in a polynucleotide chain.
• DNA, on the other hand, consists of 2 polynucleotide chains that run in opposite directions to one another.
• This gives rise to the term “anti-parallel.”
56
Nucleic Acids• The 2 anti-parallel strands of DNA
appear like a divided highway and are joined together by hydrogen bonds and Van der Waals interactions.
• H-bonds are between paired bases• Van der Waals interactions are between
stacked bases.
58
Nucleic Acids• There are certain bases that you find in
DNA and RNA.• DNA has A,T,C,G• RNA has A,U,C,G• When the bases pair together, they only
pair one way: A & T, C & G in DNA, and in RNA A pairs with U, C pairs with G.
59
Nucleic Acids
• The two strands are always said to be complementary. That is, if you know the order of one strand of DNA, you can deduce the other.
• 5’ --------> 3’
• AGGTCCG
• TCCAGGC
• 3’<-------- 5’
60
Nucleic Acids
• This feature of DNA is what makes precise copying possible.
• When cells divide, the DNA serves as a template and the new DNA that is formed is copied from it.
• When finished, there are 2 identical copies (ideally) of DNA within the cell and identical daughter cells can now form upon division.
61
Nucleic Acids• The parts of the DNA molecule that
make up the polynucleotides that encode for the amino acids can be used to show how closely organisms are related from an evolutionary standpoint.
• Molecular biologists can sequence genes and determine how much difference there is between organisms and this helps them form their tree of life.
62
Nucleic Acids
• For example, upon examination of the polypeptide chain of human hemoglobin with that of a gorilla, we only find one amino acid different out of 146.
• In gibbons, which are a tree monkey found in SE Asia, there is only a difference of 2 amino acids.
QuickTime™ and a decompressor
are needed to see this picture.
64
Lipids
• There are three classes of lipids:
• Fats (Fatty acids)– Saturated– Monounsaturated– Polyunsaturated
• Phospholipids
• Steroids
65
Lipids
• Lipids are not polymers, they are long chain fatty acids attached via a dehydration reaction to a glycerol head.
66
Lipids• Triglycerides are formed from the
condensation of three fatty acids and one glycerol.
66
http://science.halleyhosting.com/sci/ibbio/chem/notes/chpt3/triglyceride.htm
68
Lipids
• The glycerol molecule is a 3 carbon alcohol with a hydroxyl group attached to each carbon. During the reaction, the carboxyl group at the end of the fatty acid loses its hydroxyl (-OH) and attaches to the oxygen on the glycerol molecule. The -H lost from the glycerol combines with the -OH forming water.
69
Lipids• The fatty acid portion is a long
hydrocarbon usually 16 to 18 carbons in length.
• These portions are hydrophobic and this makes fats hydrophobic.
• Fatty acids can be classified as saturated or unsaturated--as we often hear about in nutrition.
70
Lipids• When they are saturated, all
of the carbon atoms contain a maximum number of H atoms n the chain. Thus they are allowed to group tightly together as we see in butter and animal fat. Most animal fat is saturated.
71
Lipids
• Unsaturated fats contain carbon atoms that are double bonded to other carbon atoms in the fatty acid chain. This creates a kink in the chain and prevents the molecules from packing closely together.
72
Lipids
• Such examples are olive oil and vegetable oil.
• The oils of fish and vegetables generally are unsaturated.
QuickTime™ and a decompressor
are needed to see this picture.
74
Lipids
• Lipids are normally used for long-term energy storage.
• Carbohydrates are short-term energy.
• Lipids are stored in adipose tissue.
74
75
Lipids
• Why lipids?– Lipids store twice the amount of energy as
proteins and carbohydrates.• 1 gram fat = 9 calories• 1 gram protein = 4 calories• 1 gram carbohydrate = 4 calories
– Compared to carbohydrates, lipids add only as much body mass as ⅙carbohydrates.
75
76
Lipids
• Why lipids?– Fats are stored as pure fat droplets whereas
glycogen is associated with water.
– Mass used for storage =
– = ⅙– This is critical for active animals that need to carry
their energy stores--bats and birds and the like.
(energy in) 1g lipids(energy in) 2g carbs + 4g associated H2O
77
Lipids Vs. Glycogen
• There is a tradeoff between glycogen and lipids.
• Lipids are lighter to carry, but glycogen can be mobilized faster.
• Glycogen is rapidly broken down and glucose is made available to cells quickly via the bloodstream.
77
78
Saturated
• Saturated fats have the maximum amount of hydrogens attached to the carbon tails.
78
http://www.agricultured.org/fats-in-food/
79
Saturated Fats
• Saturated fats are solid at room temperature.
• They are found in animal products like butter, cheese and milk.
• Palm oil and coconut oils are also high in saturated fats, but are exceptions to the “solid at room temperature” rule of thumb.
79
80
Monounsaturated
• Monounsaturated fats have one C-C double bond.
80
http://www.agricultured.org/fats-in-food/
81
Monounsaturated Fats
• Monounsaturated fats are found in plant based oils such as olive, canola, and soybean oil.
• Fish, nuts, seeds, and some vegetables are rich in monounsaturated fatty acids.
81
82
Polyunsaturated
• Polyunsaturated fats have multiple C-C double bonds.
82http://www.agricultured.org/fats-in-food/
83
Polyunsaturated Fats
• Polyunsaturated fats are commonly referred to as omegas.
• Omega-3 and Omega-6 are common examples.
• Corn, soybean, safflower oils are common sources of Omega-6.
• Soybean and canola oils, walnuts, flaxseed and fish are common sources of Omega-3.
83
84
Cis-Unsaturated Fats
• “Cis-” means, “same side.” This means that the hydrogens are on the same side of the double bond in the C-C double bond.
84
http://www.agricultured.org/fats-in-food/
85
Trans-Unsaturated Fatty Acids
• “Trans-” means “opposite.”
• This means that the hydrogens are on the opposite sides of the C-C double bond.
85http://www.agricultured.org/fats-in-food/
86
Trans-Unsaturated Fatty Acids
• Most trans fats are artificially produced by a process called hydrogenation--where extra hydrogens are added to unsaturated fats.
• Many trans fats have a straightened shape that makes them ideal for the food industry.
86
87
Trans-Unsaturated Fatty Acids
• They are not “saturated fats,” but behave like them.
• They make margarine buttery, yet soft.
• They give Crisco its properties.
• They make things such as Jif peanut butter stay solid at room temperature.
• Most food companies have recently taken trans-fats out of their products.
87
88
Health Risks Associated with Trans & Saturated Fats
• There have been many claims about the effects of different types of fats on human health.
• The main risk factor associated with these fats is that of coronary heart disease.
• In this disease, coronary arteries become partially or completely blocked by fatty deposits leading to heart attacks.
89
Health Risks Associated with Trans & Saturated Fats
• A positive correlation has been found between saturated fat intake and rates of CHD in many studies.
• Correlation ≠ causation.
• A lowered fiber intake could be responsible.
• Maasai of Kenya have a diet rich in meat, fat, blood and milk--thus a high consumption of saturated fats.
89
90
Health Risks Associated with Trans & Saturated Fats
• CHD is almost unheard of in the Maasai population.
• Diets rich in olive oil and other cis-monounsaturated fatty acids are traditionally eaten in countries around the Mediterranean. These countries populations typically have low rates of CHD.
• Genetic factors could also be responsible.
91
Health Risks Associated with Trans & Saturated Fats
• Other aspects of the diet could also be responsible for CHD rates.
• There is a positive correlation between the amount of trans-fat consumed and rates of CHD.
• Other risk factors have been tested to see if they account for the correlation; none did.
92
Health Risks Associated with Trans & Saturated Fats
• Trans-fats are probably responsible for CHD.
• In patients who died from CHD, fatty deposits in the diseased arteries were found to contain high concentrations of trans-fats.
• This lends more evidence to the causal link.
92
93
Determination of BMI Using a Nomogram
• Body Mass Index (BMI) is a tool used to screen individuals for possible weight problems.
93
BMI Status
Below 18.5 Underweight
18-6-24.9 Normal
25.0-29.9 Overweight
30.0 and Above Obese
94
Determination of BMI Using a Nomogram
• BMI =
• The units for BMI are kg/m2
94
mass in kilograms(height in meters)2
95
Determination of BMI Using a Nomogram
95
96
Determination of BMI Using a Nomogram
96
97
Phospholipids
• Phospholipids are a very important molecule that are similar to fact except that they only have 2 fatty acids attached to the glycerol head.
98
Phospholipids• The 3rd carbon is
attached to a phosphate group. The phosphate group has a negative charge and allows the phospholipid to be both hydrophobic and hydrophilic.
99
Phospholipids• The head region of the phospholipid is hydrophilic
and the tail region of the phospholipid is hydrophobic.• Thus, when phospholipids are added to water they
form a bilayer with the heads orienting out, in contact with the water and the tail regions are pointing inward away from the water.
• This forms the basis of the phospholipid bilayer that is the cell membrane.
100
Steroids
• Steroids are a final class of lipids that we’ll discuss. They are comprised of 4 fused rings and have various functional groups attached to them.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
101
Steroids
• Cholesterol is an important sterol found in animal cell membranes and is the precursor of many hormones found within the body.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
102
Steroids
Testosterone Estrogen Cortisol
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
top related