化脓菌分离鉴定过程(二) 肠道菌分离鉴定过程(二)
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化脓菌分离鉴定过程(二)肠道菌分离鉴定过程(二)
Experiment Two
化脓菌分离鉴定过程Objective掌握从临床标本中分离和鉴定化脓性球菌的原则和方法。辅助临床诊断及指导临床用药。
Identified method for pyogenic cocci
Gram-stainIsolation and culturePure culture Direct identification The antibiotic susceptibility test
Gram-stain
Morphological characteristics 葡萄球菌-球形,呈葡萄串状排列链球菌-链状排列肺炎链球菌-矛头状,成双排列,尖端向外
Staphylococci are Gram-positive cocci, typically arranged in clumps or Grape-like clusters
Isolation and culture
Cultural• Specimens planted on blood agar plates
give rise to typical colonies in 18h at 37℃
Isolation and culture colonial characteristics
• 致病性葡萄球菌• 中等大小( 1 ~ 2mm)、表面光滑、边缘整齐、不透明、有溶血环、产生金黄色色素或白色色素
• 乙型溶血性链球菌• 菌落较小( 0.5 ~ 0.75mm)、灰白色、表面光滑、有光泽、有清晰透明溶血环
• 甲型溶血性链球菌 /肺炎链球菌• 细小菌落周围产生草绿色溶血环。
纯培养( Pure culture)Materials
• Agar slope culture• Colonies on agar plate
Specimens Different specimens should be taken depending
on the kind and the process of the disease.
革兰染色
Isolation and culture
Culture medium: S.S agar Method: streak plate
Results: unpathogenetic: middle size, red, opaque Suspect colonies: small, colorless, semiopaque
Primary identification
双糖铁培养
细菌基本形态观察葡萄球菌链球菌淋病奈瑟菌大肠杆菌霍乱弧菌
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