alfred day hershey his best known experiment is hershey chase experiment concerned the replication...
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Alfred Day Hershey His best known
experiment is Hershey Chase experiment
Concerned the replication of genetic structure of viruses
4 Dec. 1908--- 22 May 1997
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1934 -1950 Teaching and research at the Department of Bacteriology1936 Held an instructorship in bacteriology and immunology 1936 - 1939 Work together with Professor J. J. Bronfenbrenner. 1938 Became assistant professor 1942 Became associate professor
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What is T2 ?What is T2 ?•The T2 phage is a strongly toxic bacteriophage and tadpole-shaped virus
• small virus that infect only bacteria
•Contain 50% DNA and 50% protein
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•Contains linear double-stranded DNA
Infect E-coli
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tail fibers of the phage attach to the bacterial cell to initiate infection
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Protein coat of the T2 carries the organ of attachment to bacteria.
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•Virus attach to the surface of their host cellVirus attach to the surface of their host cell (a)
The infective cycle of T2 in E.coil
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•Inject the DNA into the cellInject the DNA into the cell (b)
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•DNA accumulate,other genes are transcribed and translatedDNA accumulate,other genes are transcribed and translated (C)
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•DNA and protein are assembled into complete virusDNA and protein are assembled into complete virus (d)
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•The cell breaks and releases its content of virusThe cell breaks and releases its content of virus (e)
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Hershey Chase Experiment Radioactive
Sulphur (35S)
non-radioative DNA?
Radioactive Phosphorous(35P)
radioactive DNA?
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