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Agilent ChemStation for LC Systems

Installing Your ChemStation

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Installing ChemStation for LC Systems

Notices© Agilent Technologies, Inc. 1994-2003

No part of this manual may be reproduced in any form or by any means (including elec-tronic storage and retrieval or translation into a foreign language) without prior agree-ment and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Microsoft ® is a U.S. registered trademark of Microsoft Corporation.

Manual Part NumberG2170-90007

Edition07/03

Printed in Germany

Agilent Technologies Deutschland GmbHHewlett-Packard-Strasse 8 76337 Waldbronn, Germany

Software RevisionThis guide is valid for A.01.xx revisions of the Agilent ChemStation for LC Systems software, where xx refers to minor revisions of the software that do not affect the techni-cal accuracy of this guide.

Warranty

The material contained in this docu-ment is provided “as is,” and is sub-ject to being changed, without notice, in future editions. Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connec-tion with the furnishing, use, or per-formance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall control.

Technology Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accor-dance with the terms of such license.

Restricted Rights LegendIf software is for use in the performance of a U.S. Government prime contract or subcon-tract, Software is delivered and licensed as “Commercial computer software” as defined in DFAR 252.227-7014 (June 1995), or as a “commercial item” as defined in FAR 2.101(a) or as “Restricted computer soft-ware” as defined in FAR 52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or disclo-sure of Software is subject to Agilent Tech-nologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will

receive no greater than Restricted Rights as defined in FAR 52.227-19(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June 1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

Safety Notices

CAUTION

A CAUTION notice denotes a haz-ard. It calls attention to an operat-ing procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

WARNING

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indicated condi-tions are fully understood and met.

In This Guide…This guide describes how to install the initial software, how to add further instrument modules, how to configure your analytical system, and how to verify that the installation and configuration are complete and operational.

1 Installing Agilent ChemStation

This chapter provides a description of the installation process.

It gives you an overview of the complete installation procedure, starting with the PC and operating system requirements and ending with the connection of the analytical instruments.

The installation of the Agilent ChemStation software itself is described in detail in this chapter.

2 Prerequisites

This chapter lists the minimum requirements for successful operation of the Agilent ChemStation software.

It provides detailed information about the requirements that must be met to ensure proper operation of the Agilent ChemStation. Requirements include PC type and performance, network protocol, printer type, GPIB cards, and the operating systems.

3 Installing Communication Components

This chapter will give you the background information that you need in order to prepare your Agilent ChemStation hardware and operating environment.

You will have to verify that your PC meets the hardware and software requirements for the Agilent ChemStation and do the necessary adjustments where appropriate.

If you bought your Agilent ChemStation as a bundle from the Agilent Technologies Chemical Analysis Group, these steps have already been performed for you. In this case, PC hardware has already been set up for you, the GPIB card has been installed and configured, the operating system and operating environment have been preloaded on your computer’s hard-disk, and the Agilent ChemStation has been installed.

Installing ChemStation for LC Systems 3

4 Installing the Analytical Instruments

This chapter describes how to connect the HP 1050, HP 1090 and Agilent 1100 LC analytical instruments (and specific detectors) to the Agilent ChemStation using a LAN or a GPIB connection. Default GPIB address settings and firmware requirements for the LC Instruments are also described.

6 Configuring the Analytical System

The first section in this chapter describes how to configure the communication of your Agilent ChemStation software with your LC instruments using a GPIB or LAN connection with the Agilent ChemStation Configuration Editor.

The second section describes how to configure a bootp server that is required for communication using a LAN connection.

5 Checking Out the Agilent ChemStation

This chapter describes how to perform a short system integrity test.

The steps outlined in the chapter help you when validating your system installation.

7 Troubleshooting

This chapter will provide elementary troubleshooting information in case something goes wrong.

The chapter will cover the most common software start-up problems, printer problems and computer problems and how to overcome them.

8 Maintaining the Agilent ChemStation Computer

On every system, regular maintenance is mandatory to assure correct operation.

In this chapter, we will describe maintenance steps that you should perform on a regular basis. These steps include cleaning out left-over temporary files, checking the integrity of the logical and physical structure of the file system, virus-checking and regular back-ups.

4 Installing ChemStation for LC Systems

9 Upgrading From a Previous Revision of ChemStation

This chapter describes the steps required to perform upgrades from previous revisions of the ChemStation and to port your sequence, method and data files to the new architecture.

Your upgrade scenario will differ according to the ChemStation revision from which you wish to upgrade. Each scenario will be covered in a separate section in this chapter, each section covering the upgrade from the previous revision.

10 Supplementary Information


Contents

1 Installing Agilent ChemStation 15

Installation Procedure 16

Contents of the Agilent ChemStation CD-ROM 17

Agilent ChemStation Modules 17Installation Verification Utility for Installation Qualification 17Agilent ChemStation Software Status Bulletin (SSB) 17Agilent ChemStation Revision History 18I/O Libraries for the Agilent 82350 A/B GPIB Interface 18Bootp Server 18Agilent ChemStation Product Documentation 18What Is Reference Information and Where Is It? 19What Is Task-Orientated Information and Where Is It? 19What Is the Integrated Tutorial for? 19Where Can I Find the Description of Agilent ChemStation Commands and Data

Structures? 20I Upgraded from a Previous Version - How Do I Know What Has Changed Since

Then? 20

Agilent ChemStation Product Structure 21

Additional Data Evaluation Modules 22

Installing the Agilent ChemStation 23

Overview 23Installation Procedure 23

Setting Up a Shared Network-Server Based — Installation 30

What is Server-Based Installation? 30System Requirements for a Server-Based Agilent ChemStation

Installation 31


Contents

How to Perform a Server-Based Installation of the Agilent ChemStation 31Installing Agilent ChemStation System Files to the Network Server 32Activating a Shared Installation of the Agilent ChemStation for Use from a

Client PC 33Activating the XML based interface 37

Uninstalling the ChemStation 38

2 Prerequisites 41

Agilent ChemStation PC Hardware Details 42

Minimum PC Hardware Requirements for the Agilent ChemStation 43Non-HP Computers 43Printers for the Agilent ChemStation 44Advanced Power Management Not Supported with Analytical Hardware 45

Operating System Requirements 46

Number of Instruments Supported 47

3 Installing Communication Components 49

Installing LAN Communication 50

Installing TCP/IP on Windows 2000 Systems 50Installing the CAG Bootp Server Program 52

GPIB Communication 53

Instrument Compatibility Matrix 53Agilent 82341C GPIB Interface Card 54Agilent 82350 A/B GPIB Interface card 55Installing an GPIB Interface Board in the PC 56GPIB Cabling 57

Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers 58

GPIB board Configuration with the Agilent ChemStation 64

4 Installing the Analytical Instruments 65

Connecting an Agilent 1100 Series LC to the Agilent ChemStation 66


Contents

Instrument cabling 66Connecting Agilent 1100 Series Modules to the Agilent ChemStation using

GPIB 67Connecting Agilent 1100 modules to the Agilent ChemStation using LAN 70

Connecting an HP 1090 LC to the Agilent ChemStation 72

GPIB Cabling 72Remote Cable 73Setting the GPIB Address 76

Connecting HP 1050 Series Modules 77

Connecting Miscellaneous Instruments to the Agilent ChemStation 81

Setting the GPIB Addresses 81Remote Control Cabling 82

LC Instrument Firmware Requirements 83

5 Checking Out the Agilent ChemStation 87

Agilent ChemStation Installation Verification 88

Overview 88Installation Qualification 88Adding the Agilent ChemStation Installation Verification Utility 89Agilent ChemStation Installation Verification Utility Execution 89Agilent ChemStation IQ Utility Results 90Generating an Installation Verification Reference File on a Customized

Installation 93

Qualifying Operation — Agilent ChemStation Verification Test 94

6 Configuring the Analytical System 97

Using the Agilent ChemStation Configuration Editor 98

What is the Agilent ChemStation Configuration Editor? 98Starting the Configuration Editor 99What You Do 99Select and Configure the GPIB Interface 100


Contents

Configuring Paths 100

Example for LC Configurations 102

Configuring an Agilent ChemStation Instrument 102Configuring an Agilent 1100 Series LC System 103Configuring an HP 1050 LC System 105Configuring an HP 1090 LC System 108

Using the Bootp Protocol 109

What is the Bootp Protocol 109Configuring the CAG Bootp Server Program 110Configuring the JetDirect Card Using Bootp 112

7 Troubleshooting 115

Troubleshooting LAN Communication 116

Power fail reported on the Agilent ChemStation 116Frequent Buffer Overrun in Instrument Log Book 117

Troubleshooting GPIB Interface Problems 118

Resolving Agilent 82341 I/O Port Conflicts 119Resolving Agilent 82341 IRQ Conflicts 120“Autoadd Button” of IOCFG Installs Defaults Incompatible with the Agilent

ChemStation 120

Software Start-up Problems 121

An online core must be purchased in order to install additional instrument modules 121

ChemStation cannot be located on your system. You must first install Agilent ChemStation before installing an instrument 121

ChemStation online session does not finish start-up 121…key in section [PCS…] of WIN.INI not valid or specified file does not

exist 123General Protection Fault in Module... 125

Printing Problems 126

ChemStation Print Spooler hangs after an error occurred 126


Contents

Printing in Multi-Instrument configurations 126Recovering from Printer hang-ups 127Printing Messages 128

Computer Problems 130

Sporadic Hang-Ups 130System Crash in an Online Session 130Cannot create file… 130Slow Hard-Disk access or Hard-Disk activity LED is continuously

flickering 130

LC Instrument Start-up Problems 132

8 Maintaining the Agilent ChemStation Computer 135

Cleaning up Left-Over Temporary Files 136

Notes on the Usage of Long File Names 136

Maintaining the PC’s File System 137

Scanning and Repairing NTFS Volumes 137Defragmenting an NTFS Volume 137Scanning for Viruses 138

Performing Backups 139

Windows 2000/XP Backup 139Automating and Scheduling Windows 2000/XP Backup 139Travan Tape Drives 140Digital Audio Tape (DAT) Drives 140Magneto-Optical (MO) Disk Drives 140Recordable CD-ROM 141Network Servers 141

Gathering System Information 142

Gathering System Information with Windows 2000/XP 142

Using the DrWatson Utility 143

Who Is Dr. Watson? 143Dr. Watson in Windows 2000 /XP 144


Contents

9 Upgrading From a Previous Revision of ChemStation 145

Introduction 146

Importing Files from a Previous ChemStation Revision 147

Importing Sequence Files from Revisions Prior to A.03.01 147Importing Data Files 149Importing Customized Report Templates 150Delete a User Report Style 150

Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series) 151

Importing Method Files 151

Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series) 153


Upgrading from A.02.0x of the HPLC ChemStation (DOS Series) 154


Upgrading from A.03.0x of the ChemStation 155

Enhanced Integrator 155Internal Storage Format of Quantification Data 156Upgrading from A.03.0x Sequencing User Interface 156Upgrading from A.03.0x methods 157Transferring User Report Styles 158


A.05.0x Default Methods Use the Enhanced Integrator 159A.05.0x Methods May Use Enhanced Peak Purity 160






Importing HPLC ChemStation (Pascal Series) Files 167


Contents

Overview 167

Importing Pascal ChemStation files using the LIF Utility 168

Before You Start 168Converting ChemStation Data Files using the LIF Utility 170Converting Libraries from the HPLC ChemStation (Pascal Series) to the

ChemStation using the LIF Utility 171

10 Supplementary Information 175

Agilent ChemStation Revision Code Convention 176

Agilent Technologies Response Center Support Services 177

Calling the Agilent Technologies Analytical Response Center 178

Index 179


Contents


Agilent ChemStation for LC Systems Installing Your ChemStation

1Installing Agilent ChemStation

Installation Procedure 16

Contents of the Agilent ChemStation CD-ROM 17

Agilent ChemStation Product Structure 21

Installing the Agilent ChemStation 23

Setting Up a Shared Network-Server Based — Installation 30

Uninstalling the ChemStation 38

15Agilent Technologies

1 Installing Agilent ChemStationInstallation Procedure

Installation Procedure

To ensure the successful installation of the Agilent ChemStation and the connection of analytical instruments, we strongly recommend to follow the following step by step procedure.

1 Check the computer and operating systems requirements in Chapter 2, “Prerequisites”

2 Install the Agilent ChemStation software as described in, section “Installing the Agilent ChemStation” on page 23

3 If required install the drivers for GPIB communication or the bootp server as described in Chapter 3, “Installing Communication Components”

4 Install the analytical instruments as described in Chapter 4, “Installing the Analytical Instruments”

5 Configure the Agilent ChemStation software according to the chosen installation as described in Chapter 6, “Configuring the Analytical System”

6 Examine the installation as described in Chapter 5, “Checking Out the Agilent ChemStation”


Installing Agilent ChemStation 1Contents of the Agilent ChemStation CD-ROM

Contents of the Agilent ChemStation CD-ROM

Agilent ChemStation Modules

The CD-ROM contains the executable files for all modules that belong to the Agilent ChemStation product family. In order to install a module, you have to supply a license registration number valid for the module. Those numbers “unlock” the protected CD-ROM. They are included with the original product and are your proof of license.

There is a main setup program setup.exe for the Agilent ChemStation modules located in the root directory on the Agilent ChemStation CD-ROM.

Installation Verification Utility for Installation Qualification

The Agilent ChemStation comes with a utility that allows to qualify and document the completeness and integrity of a new installation. The Installation Verification Utility is installed automatically with the Agilent ChemStation.

Agilent ChemStation Software Status Bulletin (SSB)

The Software Status Bulletin is a document reflecting the results of Agilent Technologies defect logging, tracking, and repair methodology by publishing the current status of Agilent ChemStation application software products (known defects, available fixes, additional information).

The SSB is located in the SUPPORT\SSB directory of the Agilent ChemStation CD-ROM.


1 Installing Agilent ChemStationContents of the Agilent ChemStation CD-ROM

Agilent ChemStation Revision History

The revision history is targeted towards users who may have to consider revalidating their analytical data system after upgrading to a new revision of the application software. The history files are located in the SUPPORT\HISTORY directory of the Agilent ChemStation CD-ROM.

I/O Libraries for the Agilent 82350 A/B GPIB Interface

The Agilent ChemStation CD-ROM contains the version of the I/O Libraries that have been successfully tested with A.10.01 of the Agilent ChemStation. The I/O Libraries must be installed separately, as described in “Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers” on page 58.

Bootp Server

The Agilent ChemStation CD-ROM contains a bootp server setup in the directory bootp that can be used to supply the analytical instruments that are connected to a LAN with an IP address and configuration settings. This bootp server is easy to configure for the use of analytical instruments using a LAN connection. Refer to section “What is the Bootp Protocol” on page 109 for more information about the bootp server.

Agilent ChemStation Product Documentation

The Agilent ChemStation product documentation consists of paper handbooks with reference information and online documentation for task-orientated topics. The paper manuals are also located on the CD-ROM in the directory manuals, together with the required Adobe Acrobat reader.

The newly implemented XML interface is fully documented in the Agilent ChemStation XML Interface Guide, which is available as a PDF document on the ChemStation CD-ROM.


Installing Agilent ChemStation 1Contents of the Agilent ChemStation CD-ROM

What Is Reference Information and Where Is It?

With the documentation strategy for the Agilent ChemStation chosen by Agilent Technologies, all reference information, i.e. explanation of general concepts, algorithms and formulae is contained in the “Understanding” handbooks. The reference handbooks generally do not provide any task-orientated information (e.g. “How to set up a sequence”).

The Agilent ChemStation handbooks do not cover details on analytical hardware (other than those required for installation and configuration for operation with the Agilent ChemStation).

What Is Task-Orientated Information and Where Is It?

Task-orientated information describes how to perform a certain task and is therefore very context-specific. The context-specific “How-To” information is contained in the Agilent ChemStation help-system. The help-system always can be evoked by pressing the F1 function key. the Agilent ChemStation “Help”-menu, or the “Help” button available in Agilent ChemStation dialog boxes.

Access the “Help/Help Topics” menu and click the “LC Tasks” or “GC Tasks” index. Select the analytical task that you need instructions for.

What Is the Integrated Tutorial for?

Your Agilent ChemStation comes with an integrated tutorial. “Integrated” means: Rather than simulating it, the tutorial lets you work with the live Agilent ChemStation. If you cannot find the menu or button to accomplish a certain task, use the “Show Me” button from the task’s help screen to have the tutorial show you what to do by pointing the mouse cursor to the appropriate spot on the screen and pressing the appropriate menu or button for you.


1 Installing Agilent ChemStationContents of the Agilent ChemStation CD-ROM

The tutorial does not limit you to a predetermined scenario. For instance, you can use the “Setting Up an Analysis” task to define your first Agilent ChemStation method.

You start the Agilent ChemStation tutorial from the “Help/Tutorial” menu. The tasks displayed in the tutorial main screen depend on your current context, e.g. if your current Agilent ChemStation view is “Method and Run Control”, the tutorial main screen only displays tasks available in this view.

Where Can I Find the Description of Agilent ChemStation Commands and Data Structures?

The Macro Programming Guide and Commands Reference are available for advanced users and Agilent ChemStation application developers. The Commands reference information can be accessed from the Agilent ChemStation help menu (“Help/Commands”) and is structured into command categories and the command descriptions with syntax, parameters, return values along with a discussion and frequent examples.

The Macro Programming Guide is available electronically through the Agilent ChemStation help system.

I Upgraded from a Previous Version - How Do I Know What Has Changed Since Then?

When starting the Agilent ChemStation for the first time, you can select to go to the What’s new section of the help. Later on you can always go to the table of content of the help file and select new features to find out what feature have been added to the Agilent ChemStation.

A list of all defect fixes since the last revision is located in the directory support\history.


Installing Agilent ChemStation 1Agilent ChemStation Product Structure

Agilent ChemStation Product Structure

The Agilent ChemStation for GC, LC, LC/MS, CE and A/D has been designed as a series of software modules for instrument control, data acquisition, data analysis and reporting. Technique-specific single instrument modules provide control capabilities for a given separation technique, e.g. for one Gas or Liquid Chromatograph. A single instrument configuration may be expanded by adding an additional instrument control module. With this approach, one Agilent ChemStation is capable of controlling more than one analytical system, e.g. two chromatographs of the same or different separation techniques.

Please note the memory requirements for every additional instrument module (see “Minimum PC Hardware Requirements for the Agilent ChemStation” on page 43).

For further information regarding Solution Add-Ons (e.g. Purify Software, ChemAccess) please contact your local administration.

Figure 1 Agilent ChemStation Module Architecture

ChemStore C/SDatabase Module

Spectral EvaluationModule (3D)

2D Chromatography Core

A/D CE GC LC LC-MSD

Chromatography Core Module(generic)

Instrument Control Modules(optional, technique-specific)

Data Analysis Modules(optional)

Solution Add Ons


1 Installing Agilent ChemStationAgilent ChemStation Product Structure

Additional Data Evaluation Modules

The acquired data is typically two-dimensional (“2D”), i.e. by measuring detector response over time. Spectroscopic detectors can deliver three-dimensional (“3D”) data, by additionally measuring detector response over a third axis (e.g. wavelength or mass range). The optional “Spectral Evaluation Module” permits analysis and reporting of this “3D” data and is included with the CE and LC/MS modules, and optional for an LC module.

The Agilent ChemStore Database Module, a results storage and organization module may be added to all separation techniques.

The Agilent ChemStation is capable of controlling up to four “2D” instruments or up to two “3D” instruments concurrently. For LC/MS and CE, CE/MS no additional instruments are supported on the PC system.

Please note the memory requirements for each additional data evaluation module For further details on supported configurations and their PC requirements, see“Minimum PC Hardware Requirements for the Agilent ChemStation” on page 43.

Figure 2 A combination of modules results in an Agilent ChemStation for a specific technique. The example illustrates a typical configuration for LC. The driver for one LC instrument is included in the G2170AA Instrument Core Module.

ChemStore C/SDatabase Module

Spectral EvaluationModule (3D)

2D Chromatography Core

A/D CE GC LC LC-MSD

G2170AA(Agilent ChemStation for LC)

G2180AA (spectra evaluation)and


Installing Agilent ChemStation 1Installing the Agilent ChemStation

Installing the Agilent ChemStation

Overview

This section explains how to use the enclosed CD-ROM to:

• install the Agilent ChemStation for the first time

• upgrade existing Agilent ChemStation software

• install an additional Agilent ChemStation module

Installation Procedure

Use the following procedure if you are using the CD-ROM to install or upgrade the Agilent ChemStation.

Before installing the software:

• Set up the computer hardware: If you are going to connect your instruments using a GPIB connection, install an GPIB communication board in your PC as described in “Installing an GPIB Interface Board in the PC” on page 56.

• Set up the computer settings:It is recommended that the Regional options in the Control Panel of your System are set to English (US). If another location is used, the following settings are mandatory:

• Decimal symbol = . (point)

• Digit grouping symbol = , (comma)

• List seperator = , (comma)

1 Install the Windows 2000 Professional or Windows XP Professional operating system on the PC. Refer to the documentation delivered with your PC or with the operating system. Start the operating system. Please make sure that your logon does have administrative capabilities, as changes will be made to the win.ini and the Windows registry.

2 Check the partitioning scheme of your PC and ensure that the Agilent ChemStation is installed on a large enough partition and that the path to


1 Installing Agilent ChemStationInstalling the Agilent ChemStation

the data directories is configured to a large enough partition. (See “Configuring Paths” on page 100.

3 Insert Agilent ChemStation CD-ROM into the CD-ROM drive.

4 From the Start menu in the Task Bar, select Start > Run.

5 At the command line, type diskdrive:\Setup (e.g., D:\Setup), then click on OK.

If this is an installation on a new computer, proceed with step 5.

If you are upgrading your system or adding an additional module, click on Yes to have the Setup program update all existing Agilent ChemStation software on your PC. This guarantees all Agilent ChemStation instrument modules have the same version. Click on No to exit the program without installing anything.

After updating the existing Agilent ChemStation installation, you can specify whether to install additional modules such as additional instrument control or add-on software modules. If no, you may skip the following steps and start working with the new version of the Agilent ChemStation.

6 If you need to install an additional module, specify the number of the instrument that you want to add or modify.



Select an instrument number and click on “Add/Change”.

7 Select the appropriate software module from the list of available products in the “Configure Instrument” dialog and press “Add”.

8 Enter the License Registration Number for the selected module in the “New License Number” Field and press “Add”.

The applicable product and license numbers are printed on your Software Certificate and Registration Packet (see the example license registration label).



If you need to install another module for this instrument, e.g. an additional data evaluation module, proceed with step 5. If you have purchased more than one instrument control module license for this PC, click OK and proceed with step 4 to install it as an additional instrument.

9 Check that the path points to the correct partition, default is C:\. Click on Install of the Instrument Setup box to continue.

10 Click on Yes to allow the Setup program update the path statement in your AUTOEXEC.BAT file. Choose No only if you are upgrading your Agilent ChemStation installation and you have not changed your installation directory.

Figure 3 Example License Registration Label

SOFTWARE REGISTRATION LABEL FORPRODUCT NUMBER

REVISION CODE

REGISTRATIONNUMBER

G2170AA

A.10.01

DL08E8888ASAMPLE

NOTE Although Windows 2000 and Windows XP Professional ignore most entries in the AUTOEXEC.BAT file, the path entry is still used and will modify the system environment variable PATH used by the operating system.



11 The Setup program will prompt you to configure any new instruments you installed.

Click on Yes to access the Agilent Configuration Editor and configure your instruments. Click on No to exit the Setup program and return to Windows.

12 Your software installation is now complete. The Setup program made changes to your WIN.INI file and saved a copy of the original WIN.INI file in the files named WIN.XXX (i.e., WIN.000, WIN.001, WIN.002, WIN.003) in the directory “C:\HPCHEM\BACKUP”.

Remove and store your CD-ROM in a safe place. See “Installing the Analytical Instruments” on page 65 for details of how to install your analytical instruments.

CAUTION If you select No, don’t forget to add the Path statement to your autoexec.bat file manually, later. the Agilent ChemStation directory (default: C:\HPCHEM) and Agilent ChemStation system directory (default: C:\HPCHEM\SYS) must be included in your PATH setting.



Save your license numbers in a safe place. These will be required if you have to reinstall your software or for future upgrades.

13 After you install and configure the Agilent ChemStation software, be sure to exit Windows and reboot your system before starting the ChemStation so the changes in your AUTOEXEC.BAT file go into effect.


1 Installing Agilent ChemStationSetting Up a Shared Network-Server Based — Installation

Setting Up a Shared Network-Server Based — Installation

What is Server-Based Installation?

For the convenience of Agilent ChemStation system administrators who manage multiple analytical computers in a networked laboratory environment, the ChemStation software revision A.04.01 (and greater) can be installed to and run from a shared network drive. The network server holds the Agilent ChemStation system software, allowing the appropriate number of licensed Agilent ChemStation clients to execute the same Agilent ChemStation code locally. The analytical instruments are connected to the client systems. Instrument control, data acquisition and data evaluation are performed by the clients.

The shared network-server based installation of the Agilent ChemStation allows you to administer one installation of the Agilent ChemStation software, requiring you to maintain and validate the system software only once.


Installing Agilent ChemStation 1Setting Up a Shared Network-Server Based — Installation

System Requirements for a Server-Based Agilent ChemStation Installation

• Each client PC must meet the hardware and software requirements for the Agilent ChemStation.

• The PC used to install the Agilent ChemStation system software to the shared drive on the network server must be equipped with a CD-ROM drive

• The networking components installed on the client PCs must be compatible with the networking support built into Microsoft Windows.

• The client PCs must have loaded the networking components and logged on to the network server successfully.

• The client PCs must have access to the appropriate shared drives on the network server.

• The network server should be a dedicated server machine (e.g. HP Netserver) running a server operating system (e.g. Windows 2000 Server). The network protocol used should be routable (e.g. TCP/IP).

How to Perform a Server-Based Installation of the Agilent ChemStation

The server based Agilent ChemStation installation is a two-step process:

1 Install the Agilent ChemStation system software to a shared drive of the network server from one of the client systems.

2 Install and configure the Agilent ChemStation instrument configuration files on the client PCs.



Installing Agilent ChemStation System Files to the Network Server

The steps outlined below install the Agilent ChemStation system files to a shared drive on the network server. You are not required to install the Agilent ChemStation system files using the network operating system of the server.

1 Start Windows on the client PC that you wish to use to install the Agilent ChemStation system files to the network server.

2 Map a drive letter to the share on the server from each client PC. This drive mapping should be permanent.

3 Insert the Agilent ChemStation Installation CD-ROM.

4 Execute the server installation program (e.g. D:\SERVER\SETUP.EXE, where d: is the drive identifier of the CD-ROM drive).

5 In the Installation Location dialog, specify the drive and directory that will contain the Agilent ChemStation system files on the shared network drive.

S:\HPCHEM, where S: is the drive identifier of the shared network drive.

6 The server installation procedure now copies all Agilent ChemStation system files to the destination specified in the installation location dialog box.



Activating a Shared Installation of the Agilent ChemStation for Use from a Client PC

After successfully installing all Agilent ChemStation system files to a shared network drive, the corresponding Agilent ChemStation client PCs must be configured. This task must be performed on every client PC that is licensed to run the shared Agilent ChemStation system files.

1 Start the Agilent ChemStation client setup program from each client by executing S:\HPCHEM\CLIENT\SETUP.EXE, where S: is the drive identifier of the shared network drive and HPCHEM is the directory that contains the shared Agilent ChemStation system files.

2 In the Local Setup dialog box, specify the drive and directory that will contain the local client’s instrument configuration information (default: C:\HPCHEM).

NOTE Path specifications according to the Universal Naming Convention (UNC) are not supported by the Agilent ChemStation installation routine. A drive identifier must be specified, which means the network connection must be established by mapping a network drive.



Table 1 Instrument Types

Use Instrument Type For These Instruments

GC HP 5890, Agilent 6890 and Agilent 6850 gas chromatographs and autosamplers

LC HP 1050, HP 1090 and Agilent 1100 LC systems and HP 1040, HP 1046A, HP 1047A and HP 1049A special detectors

LC 3D HP 1050, HP 1090 and Agilent 1100 LC systems with diode array detectors or an HP 1040 stand-alone diode array detector (requires an additional G2180AA or G2185AA DAD spectral evaluation module license number)

CE Agilent capillary electrophoresis instrument

A/D – GC type 35900E dual channel A/D interface systems configured gas chromatographs with two injectors capable of simultaneous injections

A/D – LC type 35900E dual channel A/D interfaces



3 On each client, you can configure up to four 2D instrument sessions, provided you have the appropriate software licenses and license registration numbers.

The example given above installs a total of four instrument sessions, two LC 2D sessions, one A/D session and one GC session.

4 The directories that contain the Agilent ChemStation system software must be included in the path setting of the client PC. The Agilent ChemStation installation procedure can make the necessary adjustments for you.

You may decide to make these adjustments yourself. If you want to modify your system’s autoexec.bat yourself, select No, when prompted:

NOTE If you plan to install a multi-instrument configuration, please check the tables in the Agilent ChemStation hardware details section in this handbook on the required PC hardware and supported multi-instrument configurations.



5 Remember to view the Agilent ChemStation’s README.TXT file after the installation has completed. This file contains important last-minute information on the software that could not be included in the printed documentation.

6 Each installed Agilent ChemStation instrument session requires a valid license registration number, otherwise you will not be able to control the corresponding instrument online. The license registration numbers are attached to the Software Certificate and Registration Packet envelope included with the delivered Agilent ChemStation software (see Figure 3 on page 27).

7 In order to activate the changes made to your system initialization files, you need to exit all applications and restart your computer.

8 Use the Agilent ChemStation Configuration Editor to set up your instrument sessions (see Chapter 6, “Configuring the Analytical System”).



Activating the XML based interface

In order to activate XML support, you need to make changes to the WIN.INI file. For full details see the Agilent ChemStation XML Interface Guide on the ChemStation CD-ROM.


1 Installing Agilent ChemStationUninstalling the ChemStation

Uninstalling the ChemStation

In some cases you might need to uninstall the ChemStation, for example, if you wish to install it in a different location. To completely remove a ChemStation installation please proceed with the following steps.

1 If the ChemStation is running close all sessions and reboot the computer.

2 Backup your ChemStation data, methods and sequences and if necessary any customized macros such as user.mac. To do this store the following directories on your backup device or in a save location:

• \HPCHEM\1\ contains all data, methods and sequences for instrument 1




• \HPCHEM\REPSTYLE contains your customized reports

• \HPCHEM\SPECLIBS contains your spectral libraries

• \HPCHEM\CHEMSTOR contains local ChemStore databases

3 Remove the complete HPCHEM folder from your hard disk.

NOTE If any other products belonging to the ChemStation Plus Family are installed, which require to be uninstalled using the standard Windows uninstall procedure (Control Panel > Add/Remove programs) such as ChemStore or ChemAccess, please uninstall these products using the Windows routine prior to deleting the HPCHEM folder and modifying Win.ini. Please refer to the corresponding software documentation of the ChemStation Plus product for details.


Installing Agilent ChemStation 1Uninstalling the ChemStation

4 Select Start > Run > and type Sysedit. Select OK and choose Win.ini. Locate the general [PCS] section and the instrument specific [PCS,x] sections where x represents the instrument number(s) ranging from 1 to 4. Remove these sections completely. Choose Save from the File menu.

NOTE Be careful when editing the win.ini. Do not remove any other sections apart from the ones mentioned above.

NOTE A backup copy of Win.ini is automatically stored as win.syd in your local windows directory. If you need to restore the original Win.ini because you suspect any startup problems originating from your change rename the current Win.ini to, for example, Win.old and Win.syd to Win.ini.


1 Installing Agilent ChemStationUninstalling the ChemStation



2Prerequisites

Agilent ChemStation PC Hardware Details 42

Operating System Requirements 46

Number of Instruments Supported 47


2 PrerequisitesAgilent ChemStation PC Hardware Details

Agilent ChemStation PC Hardware Details

This section states the PC hardware and operating system requirements that must be met for successful installation and operation of the Agilent ChemStation.

The Agilent ChemStation A.10.0x and greater is supported on personal computers with an Intel Pentium III 600 MHz (or higher). Depending on the type of GPIB interface one ISA or PCI slot is required. For details about GPIB interface requirements refer to the section “GPIB Communication” on page 53. If a LAN connection is used for the analytical equipment, a LAN interface supported by the operating system is required and the Microsoft TCP/IP protocol must be installed. All PC hardware and peripherals must be listed in the Microsoft Hardware Compatibility List (HCL) which is available from the Microsoft home page on the world wide web (http://www.microsoft.com). If your PC hardware is not listed in the HCL, the system may not work correctly with the Agilent ChemStation software.


Prerequisites 2Agilent ChemStation PC Hardware Details

Minimum PC Hardware Requirements for the Agilent ChemStation

Non-HP Computers

The Agilent ChemStation has been designed to successfully run on a wide range of compatible personal computers equipped with accessories and peripherals that adhere to the programming standards for the Intel PC platform and Microsoft Windows operating systems.

However, Agilent Technologies has tested the Agilent ChemStation software mainly on Agilent Technologies equipment. All configuration information listed in this manual applies to Hewlett-Packard/Compaq Kayak, Vectra and EVO computers and may not be optimized for other vendor’s PCs. The standard configuration of the GPIB interface, for instance, may conflict with the memory configuration of a non-HP computer.

Additional accessory interface boards may cause conflicts of hardware related resources (I/O ports, interrupt settings, DMA channels).

Table 2 Minimum PC Hardware Requirements for Windows 2000 / XP-Based Systems

Processor Pentium III 600 MHz

Screen resolution Super VGA (800 × 600)

Mass storage 4 GB Hard-Disk and CD-ROM

Minimum Memory for Single Instrument (2D) 128 MB of RAM

Minimum Memory for Single Instrument (3D) 256 MB of RAM

Minimum Memory for Multi Instruments 256 MB of RAM

Single instrument configuration with Agilent ChemStore C/S database module (rev. B.02.0x),

256 MB of RAM

Multi instrument configuration with Agilent ChemStore C/S database module (rev. B.02.0x),

256 MB of RAM

NOTE Generally a maximum of four instrument systems may be configured to each Agilent ChemStation, but there is a limitation on the number of individual modules connected to the Agilent ChemStation PC. Refer to “Number of Instruments Supported” on page 47


2 PrerequisitesAgilent ChemStation PC Hardware Details

For a non-Hewlett-Packard computer, use the setup utility program supplied by the manufacturer to configure your computer and check the documentation supplied with it and the accessories to eliminate resource conflicts in your PC’s setup, especially regarding the configuration of the GPIB interface. In case of hardware conflicts, see “Resolving Agilent 82341 I/O Port Conflicts” on page 119.

Printers for the Agilent ChemStation

The Agilent ChemStation has been designed to work with printers that are compatible with the operating system. Printers may be attached to a local (preferably parallel) or networked port on the PC. Serial port printers are supported by the operating system but may exhibit speed performance limitations. Networked printers must be shared by a network server running a network protocol supported by the Microsoft operating system.

We recommend printers that are capable of interpreting an escape code language (e.g. PCL) or page description language (e.g. PostScript). Host-based printers (e.g. GDI or PPA printers) impose more printer processing tasks on the CPU and are not recommended for use with Agilent ChemStation on-line sessions.

For best printing results with your Agilent ChemStation, use HP LaserJet printers. High performance HP DeskJet printers may also be used if the amount of printing required is low. Check the readme.txt files for information on recommended printer driver versions.

Agilent Technologies has not tested all printer and printer driver combinations that are supported in the Windows environment. Print performance and results may vary on other manufacturer’s printers and appropriate drivers.

The printers listed in Table 3 on page 45 have been successfully tested at the time this handbook was printed.


Prerequisites 2Agilent ChemStation PC Hardware Details

Advanced Power Management Not Supported with Analytical Hardware

The BIOS and operating systems of many modern PCs supports Advanced Power Management (APM). After a specified idle time, the BIOS will switch the system to stand-by by turning off the computer display, hard disk and other devices, reducing the output of the PC’s power supply and the internal clock frequency to save energy.

The reduction in internal clock speed and hard disk slow down can render the PC incapable of handling the real-time requirements of instrument control and data acquisition. Typically, this results in overflows of the internal instrument buffers, or, (in other words) data loss. APM is not supported in systems used for on-line operation of analytical hardware.

Table 3 Printer Successfully Tested with Agilent ChemStation A.10.01

Printer Model Driver comment

HP Laserjet 2100 HP PCL 6 Driver

HP LaserJet 4050 HP PCL 5e Driver

HP LaserJet 4200 HP PCL 5c Driver

HP Business InkJet 2250c HP PCL

NOTE Please note that this list is not comprehensive and does not include printers and printer drivers made available after the release of this handbook. If your printer is not listed here, it does not mean it does not work with the Agilent ChemStation, but it was not tested.

NOTE We recommend using Hewlett-Packard LaserJet. Due to their limited print-performance (< 8 pages per minute) and paper tray capacity (less than 150 pages), some low-end printers may not be suitable for unattended high data-throughput operation of the Agilent ChemStation, e. g. in multi-instrument configurations or when running sequences with many injections and relatively short run-times (e.g less than 5 minutes run-time), even if listed in Table 3.


2 PrerequisitesOperating System Requirements

Operating System Requirements

You will need the Windows 2000 Professional (Service Pack 2 or greater) or Microsoft Windows XP Professional (Service Pack 1a) operating system. For up to date information on supported operating systems please contact your local service and support center.

If you use a LAN to connect to the analytical instruments (Agilent 1100 only), the Microsoft TCP/IP protocol must be installed and configured.

Window 2000 Professional or Windows XP Professional are required for the Agilent ChemStation data acquisition and analysis software. The Microsoft Server operating systems (NT Server and Windows 2000 Server) are not supported for ChemStations except as application servers when using the Agilent ChemStation in network-server based installation (see “How to Perform a Server-Based Installation of the Agilent ChemStation” on page 31).

NOTE Windows 95 or Windows 98, Windows ME and Windows NT are not supported with this version of the ChemStation.

NOTE The HP 82335 card is not supported. The Agilent 82341C is supported on Windows 2000 only and the Agilent 82350 A/B cards are supported on Windows 2000 and Windows XP Professional, for all analytical hardware except the instruments not designed for GPIB communication. See Table 7 on page 53 for details.


Prerequisites 2Number of Instruments Supported

Number of Instruments Supported

A maximum number of 4 instruments can be configured on a single ChemStation PC. Each instrument can consist of several modules, like Agilent 1100 LC modules, HP 1090 mainframe with DAD, HP 1050 modules, etc. The maximum number of instruments is reduced according to Table 4.

There is also a limitation on the total number of analytical modules connected to the Agilent ChemStation. Generally a maximum number of approximately 12 modules can be connected, depending on the exact configuration. The following modules count as 2 modules: Agilent 1100 DAD, Agilent 1100 FLD, Agilent 1100 VWD and the Agilent 6890 GC. Table 5 gives you an example for a possible configuration, where it would not be possible to add another detector.

Table 4 Maximum Number of Instruments

Description Number of instruments

ChemStore C/S database 3

2 modules that can acquire on-line spectra, like DAD or FLD 2

Agilent LC/MSD module 1

Agilent CE module G1600A 1

Table 5 Maximum Number of Modules

Agilent 1100 system A Agilent 1100 system B

Binary pump Binary pump

Variable wavelength detector Diode array detector

Autosampler Thermostatted autosampler

Thermostatted column compartment Thermostatted column compartment

NOTE Please contact your Agilent representative, to check if your exact configuration is possible, especially when you configuration is coming close to 12 modules.


2 PrerequisitesNumber of Instruments Supported



3Installing Communication Components

Installing LAN Communication 50

GPIB Communication 53

Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers 58


3 Installing Communication ComponentsInstalling LAN Communication

Installing LAN Communication

If you decide to connect your instrument using a standard LAN connection, you must ensure proper communication between the PC and the analytical instruments. The communication uses the TCP/IP protocol, which needs to be installed as a network protocol on your PC. For the configuration of the JetDirect card that is used to connect the analytical instrument to the LAN, the boot strap protocol is used, which requires a bootp server.

This chapter outlines the installation of the TCP/IP protocol on Windows 2000 and Windows XP systems. Windows 2000 and Windows XP systems will typically come with TCP/IP pre-installed. Instructions are also included for the CAG bootp server program required to configure TCP/IP parameters for your instrument.

Installing TCP/IP on Windows 2000 Systems

1 From the Start menu in the Task Bar, select Start -> Settings -> Control Panel.

2 Open Network and Dial-up Connections, select Local Area Connection, and use right-mouse click to access the properties.

3 In the General tab select the Internet Protocol TCP/IP and choose Properties.

4 Select Use the following IP address, to ensure the system does not use DHCP address (see “DHCP Server” on page 117 for details).

5 Ask your IT department for a valid IP address, gateway, subnet mask, DNS and WINS server, if your PC is connected to a site network. If you are setting up your own internal network and are not connected to any other network, you may use the example settings from Table 6. Gateway, DNS and WINS IP addresses do not need to be configured in this case, as you do not connect to any other part of a network. Figure 4 shows an example of the TCP/IP configuration on Windows 2000.


Installing Communication Components 3Installing LAN Communication

6 Select OK to finish the configuration.

Table 6 Example IP addresses

PC number IP address Subnet mask

1 10.1.1.100 255.255.255.0

2 10.1.1.101 255.255.255.0

3 10.1.1.102 255.255.255.0

... .... 255.255.255.0

Figure 4 TCP/IP Configuration Screen


3 Installing Communication ComponentsInstalling LAN Communication

Installing the CAG Bootp Server Program

If there is already a bootp server running in your network, contact your system administrator with the information supplied in “Using the Bootp Protocol” on page 109. If you wish to use a local bootp server, proceed as outlined below, to install the CAG bootp server program.

1 From the Start menu in the Task Bar, select Start / Run.

2 At the command line, type diskdrive:\bootp\BootpInstall (e.g., D:\bootp\BootpInstall), then click on OK.

3 Follow the instruction on the screen for the installation of the CAG bootp server program. It is recommended that you read the readme.txt file, that might contain last minute information not included in this manual.

4 The CAG bootp server program can now be started using the Bootp icon from the Agilent ChemStations group in the start menu.

NOTE Please note that the CAG bootp server program always starts as an icon in the taskbar (as if you hit the minimize button of the program window).


Installing Communication Components 3GPIB Communication

GPIB Communication

Analytical instruments that communicate with the Agilent ChemStation via GPIB require a GPIB board to be installed in your computer.

You can either use an Agilent 82341C (16-bit) high-performance GPIB interface card, which is an ISAcompatible accessory card or an Agilent 82350 A/B PCI high-performance GPIB interface card.

Instrument Compatibility Matrix

The following table helps you to select the correct GPIB interface for your system.

NOTE The HP 82335 model of the GPIB interface is not supported by Agilent ChemStations, revision A.07.01 and higher.

Table 7 GPIB and Analytical Hardware Compatibility Matrix

Instrument Type Agilent 82341C Agilent 82350

HP 1090 / HP 1040 LC Yes Yes

HP 1050 LC Yes Yes

Agilent 1100 LC Yes Yes

HP 5890 GC Yes Yes

Agilent 6890 GC Yes Yes

Agilent 6850 GC No No

Agilent G1600A (CE) Yes Yes


3 Installing Communication ComponentsGPIB Communication

Agilent 82341C GPIB Interface Card

The Agilent 82341C is an ISA- compatible GPIB interface card. It uses an I/O address range and an interrupt (IRQ) line.

The Agilent 82341 I/O base address is selected using switches 1 through 4 of the DIP switch, see Figure 5. To change the base address, power down the computer, remove the GPIB interface card, re-set the DIP switches, re-insert the card, and power up your computer. Run I/O Config again to configure the GPIB interface. In most cases, you will not need to change the default switch setting. However, if the default setting conflicts with other interfaces you have in your computer, use Table 8 on page 55 to select the desired address range to resolve the conflict.

CAUTION The GPIB board is static sensitive and should be handled with care so as not to damage it. Be sure to hold the board by the edges and do not touch the electrical components. Use anti-static equipment, such as a grounding mat and wrist-straps, during installation.

Figure 5 DIP-Switch Settings of the Agilent 82341 GPIB Interface



Agilent 82350 A/B GPIB Interface card

The Agilent 82350 is a PCI GPIB interface card. Therefore no additional settings such as changing the I/O base address are required.

Table 8 Agilent 82341C Switch Settings

Switches I/O Base Address I/O Address Range Used

1 2 3 4 (Hexadecimal) (Hexadecimal)

0 0 0 0 250 (default) 250–257

1 0 0 0 270 270–277

0 1 0 0 350 350–357

1 1 0 0 370 370–377

0 0 1 0 220 220–227

1 0 1 0 280 280–87

0 1 1 0 390 390–397

1 1 1 0 380 380–387

NOTE The selected I/O address ranges must not conflict with other I/O interfaces installed in your computer, including products of other manufacturers (for example LAN interfaces). Refer to the documentation for the other interfaces and Table 8 to select unique addresses for all the interfaces in your computer.

NOTE Because the Agilent 82341C interface is I/O port-mapped and not memory-mapped, it is not necessary to exclude address ranges for memory management software on your computer.


3 Installing Communication ComponentsGPIB Communication

Installing an GPIB Interface Board in the PC

Since these boards can be installed in several different computers, the following instructions are general. If you have difficulties during the installation, consult your computer manual or dealer.

1 Turn off and unplug your computer, then remove the computer’s cover(s).

2 Select any empty ISAcompatible or PCI slot depending on the type of GPIB board. It is best not to place the GPIB board next to a graphics adapter. As the GPIB connector is wider than a typical PC slot, you may have to leave the slot(s) adjacent to the GPIB interface empty.

3 If you have more than one GPIB board in your computer, or if some other system setting conflicts with the resources associated with the default factory setting for the GPIB board, be sure to reset the switch settings to avoid resource conflicts as described above (only applicable for the Agilent 82341C card).

4 Write down the slot number you will use and store it for later use. Some EISA computers require slot number and board type information after installation. For computers that require this information, refer to the appropriate installation documentation.

5 Loosen the mounting screw and remove the selected empty slot’s rear plate.

6 Holding the board by its edges, insert the board into its slot. Make certain that the board’s edge connector is fully seated. Lock the board into place with the mounting screw.

7 Replace the computer's cover(s). Plug in, and restart the computer.

WARNING Turn off and unplug your computer and all attached electrical devices before removing any covers.



GPIB Cabling

When connecting GPIB devices together, there are several basic rules that should be observed.

1 Whenever possible, turn off and unplug the computer and all attached devices before the GPIB cables are installed.

2 Before connecting any analytical instrument to an GPIB cable, consult the documentation supplied with each device and determine its GPIB address. No two devices connected to the Agilent ChemStation may have the same address. Alter their addresses as necessary to avoid any duplication. Write down each GPIB address. This information will be needed later.

3 Try to use short GPIB cables that are two meters long or less.

GPIB cable (0.5 m) (10833D)GPIB cable (1.0 m) (10833A)GPIB cable (2.0 m) (10833B)GPIB cable (4.0 m) (10833C)

4 Connect one end of an GPIB cable to the computer’s GPIB connector.

5 Be certain that you properly tighten all GPIB connectors. A poor connection causes errors which are difficult to diagnose.

6 Connect GPIB devices in a chain. A chain occurs when an GPIB device is connected to the next GPIB device, and it is, in turn, connected to the next, and so on. Avoid star configurations (connecting all of the devices to a central point).

WARNING According to the IEEE 488 specification for the General Purpose Instrument Bus (GPIB), the bus is not designed for dynamic configuration. Cycling power on an GPIB instrument that is connected to the bus while other GPIB instruments are actively communicating to the GPIB controller can induce electrical spikes that may potentially corrupt the GPIB protocol. In extreme cases. this may require cycling power on all instruments, including the GPIB controller (i.e. typically the Agilent ChemStation).


3 Installing Communication ComponentsConfiguring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

After installing the GPIB board in the computer, it is necessary to install the corresponding driver and configuration software, located on the Agilent ChemStation CD-ROM in the “\GPIB” directory. An IO Libs installing guide can be found on the CD-ROM as well. To install the I/O Libraries proceed with the following steps:

1 Using the Windows Explorer, locate and start ‘Iolibs.exe’ in the \GPIB directory of your ChemStation CD-ROM. Follow the instructions in the installation Wizard (see Figure 6). This will install the Standard Interface Control Library (SICL) on your computer, update the registry and add a new Program Group called “Agilent I/O Libraries”.

NOTE The Agilent ChemStation A.10.01 and greater requires version L.02.00.01 of the I/O Libraries. These libraries are supported under both Windows 2000 and Windows XP.


Installing Communication Components 3Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers

2 Choose Next/Yes to accept the License Agreement and to view the Readme Information. With the next step the Selection Screen for the Installation is coming up.

3 Choose Full installation for installing the SICL libraries and VISA components (see Figure 7). Select Next and the system will start the installation of the SICL and VISA components of the I/O Libraries.

Figure 6 The GPIB Driver Installation



4 After the installation has been successful finished, select Run IO config to configure the IO interface card.

Figure 7 Choose the full SICL and VISA installation.



5 From the list of available interface types choose the appropriate one, i.e. either ”82341 ISA GPIB” if you have an Agilent 82341C GPIB board installed or “82350 PCI GPIB” in case you have the Agilent 82350 board installed (see Figure 8).

6 Select Configure. The configuration utility, “IOCFG.EXE”, now attempts to detect the selected GPIB interface in the PC in order to assign the correct resources to the card.

Figure 8 Selecting the appropriate interface type in the I/O Config Screen

NOTE If the GPIB card is not detected in your PC although it is installed, proceed with the section “Troubleshooting GPIB Interface Problems” on page 118. Or refer to the Installation Instructions coming along with the ChemStation CD-ROM.



7 Set the SICL Interface Name to “hp82341”, the Bus Address to “30” and the “System Controller” flag as shown in Figure 9. Please note that the appearance of the dialog slightly differs depending on the type of GPIB board installed.

8 Select OK. The configured GPIB board should now be listed under “Configured Interfaces” in the Interface Configuration Screen (Figure 10 on page 63).

NOTE You need to type in the SICL interface name hp82341 with lower case letters.

Figure 9 Configuring the GPIB Interface for Use with the Agilent ChemStation

NOTE In order to be recognized by the ChemStation the SICL Interface Name has to be “hp82341” even if you have an Agilent 82350 GPIB Interface Board installed.



9 You have now successfully configured the GPIB Interface board. Restart the the computer to finish the installation.

The interface configuration screen can be accessed from the I/O Libraries Program Group by selecting I/O Config.

CAUTION If you replace an Agilent 82341 board with an Agilent 82350 board or vice versa first remove the configuration using the I/O Config Utility from the I/O Libraries Program Group before exchanging the board. After the replacement you must rerun the “IOCFG.EXE” utility to configure the correct drivers for the installed board.

Figure 10 The Interface Configuration Screen now shows the configured GPIB Board.

CAUTION Do not attempt to start the I/O Config Utility or Visa Assistant while the ChemStation is running.



GPIB board Configuration with the Agilent ChemStation

Use the following values to configure the Agilent 82341 or Agilent 82350 GPIB interface for use with the Agilent ChemStation. The available fields depend on the type of interface installed. See Figure 9 on page 62.

SICL InterfaceName

The SICL Interface Name is the symbolic name used by the Agilent ChemStation to identify and address this GPIB interface. The default Interface Name used by the Agilent ChemStation is “hp82341”, without the quotes. The same symbolic name has to be used for both interface types.It needs to be typed in lower case.

VISA InterfaceName

The default VISA Interface Name is “GPIB0” (this setting is not used by the Agilent ChemStation and thus does not need to be altered).

Logical Unit The Logical Unit number is used by the Standard Interface Control Library (SICL) to uniquely identify this GPIB interface. The Logical Unit number is an integer in the range of 0-10000. The Agilent ChemStation always uses the default value of “7”.

Interrupt Line(Agilent 82341

only)

The Interrupt Line must be reserved for exclusive use by this GPIB interface. The I/O Configuration Application automatically chooses an available interrupt for the card.

If you add additional accessory cards after the GPIB card was installed, you must make sure that these cards do not use the resources reserved for the GPIB card.

Bus Address The Bus Address determines the address of this GPIB interface on the GPIB bus. The Agilent ChemStation uses Bus Address “30” for the System Controller.

System Controller The System Controller determines which bus devices talk and listen. Each device connected to the GPIB bus must have a unique GPIB bus address and one (and only one) device acting as the System Controller. In Agilent ChemStation installations, the System Controller is always the GPIB board installed in the PC.



4Installing the Analytical Instruments

Connecting an Agilent 1100 Series LC to the Agilent ChemStation 66

Connecting an HP 1090 LC to the Agilent ChemStation 72

Connecting HP 1050 Series Modules 77

Connecting Miscellaneous Instruments to the Agilent ChemStation 81

LC Instrument Firmware Requirements 83


4 Installing the Analytical InstrumentsConnecting an Agilent 1100 Series LC to the Agilent ChemStation

Connecting an Agilent 1100 Series LC to the Agilent ChemStation

You can connect the following modules of the Agilent 1100 Series LC to your Agilent ChemStation using either LAN or GPIB:

• Agilent 1100 autosampler (ALS)

• Agilent 1100 well plate autosampler (WPS)

• Agilent 1100 thermostatted autosampler (ALS)

• Agilent 1100 pumping system (PMP)

• Agilent 1100 diode array detector (DAD)

• Agilent 1100 multi wavelength detector (MWD)

• Agilent 1100 variable wavelength detector (VWD)

• Agilent 1100 fluorescence detector (FLD)

• Agilent 11000 refractive index detector (RID)

• Agilent 1100 thermostatted column compartment (TCC)

• Agilent 1100 fraction collector (AS/PS)

• Agilent 1100 micro fraction collector

• Agilent 1100 vacuum degasser

Instrument cabling

The cabling setup of the Agilent 1100 system is the same for LAN and GPIB connection. Installation and interconnection of your Agilent 1100 Series LC modules is described in more detail in the Agilent 1100 Series Module reference manual supplied with each module.

• Connect a controller-area network (CAN) cable between each of your Agilent 1100 modules except the vacuum degasser. One CAN cable is included with each Agilent 1100 module.

• Connect a remote cable (part number 5061-3378) between the Vacuum Degasser and one other Agilent 1100 module.


Installing the Analytical Instruments 4Connecting an Agilent 1100 Series LC to the Agilent ChemStation

• If you are using the GPIB connection, connect the GPIB cable between one of the Agilent 1100 modules and the GPIB interface card in your computer.

• If you are using the LAN connection, connect the Agilent 1100 module with the JetDirect card inserted to your LAN component, using the appropriate EtherTwist 10BaseT cable. See sections “Single instrument installation” on page 70 and “Multiple instrument installation” on page 70 for details.

Connecting Agilent 1100 Series Modules to the Agilent ChemStation using GPIB

You can also use HP 1050 modules in combination with the Agilent 1100 Series modules (see “Including HP 1050 Series Modules” on page 67) when using a GPIB connection.

Including HP 1050 Series Modules

To use HP 1050 Series modules with your Agilent 1100 Series system:

• Connect a remote cable from one of the HP 1050 Series modules to one of the Agilent 1100 Series modules

• Connect an GPIB cable from one of the HP 1050 Series modules to theGPIB cable on the Agilent 1100 Series module with the GPIB cable connected.

• Connect the HP 1050 Series modules as described in “Connecting HP 1050 Series Modules” on page 77.

NOTE We recommend connecting the GPIB cable to an Agilent 1100 detector. If no Agilent 1100 detector is included in the system, connect it to the Agilent 1100 pumping system.

NOTE We recommend inserting the JetDirect card into a Agilent 1100 detector. If no Agilent 1100 detector is included in the system, use the Agilent 1100 pumping system.



Setting the GPIB Address

The Agilent 1100 modules are shipped with the default GPIB addresses as shown in Table 9.

Table 9 Agilent 1100 Default GPIB Addresses

Agilent 1100 Module Default GPIB Address

Autosampler (ALS) 28

Isocratic pump 22

Quaternary pump 22

Binary pump 22

Capillary pump 22

Diode array detector (DAD) 26

Multi wavelength detector (MWD) 26

Fluorescence detector (FLD) 23

Refractive index detector (RID) 29

Variable wavelength detector (VWD) 24

Thermostated column compartment 27



Figure 11 Agilent 1100 System

1. Solvent Cabinet2. Vacuum Degasser3. Remote Cable (5061-3378)4. Pump5. Autosampler6. Controller Area Network (CAN) bus cables (5181-1516) included with each Agilent 1100 module7. Column Compartment8. Detector9. 10833B GPIB cable10. Agilent ChemStation11. Centronics cable12. Printer



Connecting Agilent 1100 modules to the Agilent ChemStation using LAN

This section gives a brief description on how to setup a dedicated mini LAN for the analytical instruments. If you are going to connect to an existing LAN, please contact your system administrator for further assistance.

Single instrument installation

In a single instrument installation, only one instrument can be connected to the PC and the PC can not be connected to a LAN. In this configuration, use the black 10 ft. long crossover twisted pair cable 5183-4649, supplied with the Agilent ChemStation software, it can only be used for the direct connection of a single instrument to the PC.

Multiple instrument installation

If you want to connect more than one instrument to the Agilent ChemStation PC or if the PC needs to connect to a network, a hub (for example the G2402A 12 port hub) is required. The hub G2402A comes with one standard 8m EtherTwist 10BaseT cable G1530-61480 that is used for the connection to the PC. Use the standard EtherTwist 10BaseT cable G1530-61480 provided with the JetDirect cards G1846A to connect the hub to the instruments.

NOTE A LAN connection requires the Agilent 1100 modules to have firmware version A.03.80 or higher and main boards GXXXX-66520 (exchange board GXXXX-69520), were XXXX denotes the part number of the Agilent 1100 module.

NOTE For direct single instrument connection you can only use the cross-over cable 5183-4649 supplied with the Agilent ChemStation software. Do not use the standard Ethertwist 10BaseT cable supplied with the JetDirect card G1846A.

NOTE Do not use the cross-over cable 5183-4649 in an installation with multiple instruments.



Setting the IP Address of the Agilent 1100 module

The IP address of the JetDirect card inserted to the Agilent 1100 module can not be set from the Agilent 1100 system. A bootp server is required, that provides the JetDirect card with an appropriate IP address, each time it is started. Refer to section “Installing the CAG Bootp Server Program” on page 52 and section “Using the Bootp Protocol” on page 109.

Figure 12 LAN connection using a hub

1. Personal computer2. Network hub3. InstrumentsUse standard twisted pair LAN cables for the connections.

2

3

1

NOTE You need to ensure that the bootp server is running before the instrument is switched on, as the configurational data is only loaded to the JetDirect card when the module is switched on.


4 Installing the Analytical InstrumentsConnecting an HP 1090 LC to the Agilent ChemStation

Connecting an HP 1090 LC to the Agilent ChemStation

You can connect the following HP 1090 Liquid Chromatograph components to your Agilent ChemStation:

• HP 1090 LC Mainframe This includes injector, pump, oven, and optionally a filter photometric detector (FPD), and

• HP 1090 diode array detector

Installation of your HP 1090 is described in the Installation Guide contained in the Guide Books for the HP 1090 (part number 01090-90214).

GPIB Cabling

1 Connect an GPIB cable from the GPIB connector at the rear of the HP 1090 to the GPIB connector on the diode array detector (DAD).

2 Connect an GPIB cable from the GPIB connector on the DAD to the GPIB connector on your computer.

The following GPIB cables are available:

A 10833B cable is included with your HP 1090 Series II system and a 10833D cable is included with your HP 1090 DAD.

NOTE You can also use HP 1050 detectors in combination with the HP 1090 LC, see Figure 13 on page 73.

Part Number Length

10833A 1 m

10833B 2 m

10833D 0.5 m


Installing the Analytical Instruments 4Connecting an HP 1090 LC to the Agilent ChemStation

Remote Cable

Each analytical component of the system must be connected with a remote cable (part number 01040-60202) so that time-critical events, such as injections, can be coordinated. For example, with an HP 1090 system fitted with a diode array detector (DAD), you must connect a remote cable between the DAD and the rear of the HP 1090.

Some typical HP 1090 system configurations are shown in Figure 13 and Figure 14 on page 74.

NOTE A remote control cable (part number 01040-60202) and an GPIB cable (part number 10833B) are included with the diode array detector when ordered as an option to an HP 1090 system.

Figure 13 HP 1090 Win Liquid Chromatography System

1. HP 1090 Series II/M2. Remote cable (01040-60202) included with HP 1090M #0833. 10833D GPIB Cable (0.5 m) included with HP 1090M #0834. Agilent ChemStation5. Centronics cable6. Printer7. 10833B GPIB Cable (2 m)



Figure 14 HP 1090 Win LC System (Includes FLD and A/D Converter)

1. APG remote cable (5061-3378)2. Remote cable (01040-60202) included with HP 1090M #0833. HP 1090 Series II/M4. 10833D GPIB Cable (0.5 m) included with HP 1090M #0835. 10833B GPIB Cable (2 m) included with Agilent ChemStation6. Agilent ChemStation7. Centronics cable8. Printer9. 10833B GPIB Cable (2 m) included with Dual-Channel Interface10. Two signal cables (option to Agilent 35900E)11. 10833A GPIB Cable (1 m) included with FLD #30512. HP 1046A Fluorescence Detector13. APG remote cable included with FLD14. Agilent 35900E Dual-Channel Interface


Installing the Analytical Instruments 4Connecting an HP 1090 LC to the Agilent ChemStation

An example of a mixed HP 1090 and HP 1050 system configuration is shown in Figure 15.

Figure 15 HP 1090 and HP 1050 System: Multiple Instrument Version

1. HP 1090 Series II/M2. Remote cable (01040-60202) included with HP 1090M #0833. 10833D GPIB Cable (0.5 m) included with HP 1090M #0834. 10833B GPIB Cable (2 m)5. HP 1050 Pumping System6. Remote Cable (5061-3378) included with each HP 1050 module7. 10833A GPIB Cable (1 m) included with each HP 1050 module8. HP 1050 DAD9. HP 1050 Autosampler10. Agilent ChemStation11. Centronics cable12. Printer



Setting the GPIB Address

The HP 1090 LC instrument and configurable modules are shipped with the default GPIB addresses listed in Table 10.

If you configure more than one module of the same type, you must change the default settings so that each module has a unique GPIB address. For further details, refer to the instrument handbooks.

Table 10 HP 1090 Default GPIB Addresses

HP 1090 Module Default GPIB Address

HP 1090 Mainframe 14

HP 79883 DAD 15

NOTE Instructions to change the GPIB address of your HP 1090 system can be found in the Installation Guide included with the Guide Books for the HP 1090 (part number 01090-90214).


Installing the Analytical Instruments 4Connecting HP 1050 Series Modules

Connecting HP 1050 Series Modules

You can connect the following modules of the HP 1050 Series LC to your Agilent ChemStation:

• HP 1050 variable wavelength detector (VWD)

• HP 1050 multiple wavelength detector (MWD)

• HP 1050 pumping systems (PMP)

• HP 1050 autosampler (ALS)

• HP 1050 diode array detector (DAD)

Installation of your HP 1050 Series LC modules is described in Chapter 1 of the Installation and Maintenance handbook contained in the instruction kit supplied with each module.

To connect the HP 1050 Series modules together to make a complete system, see the Getting It All Together handbook contained in the instruction kit supplied with each module.

NOTE You can also use HP 1050 detectors in combination with the HP 1090 LC, see Figure 13 on page 73.

Table 11 HP 1050 Handbooks

Instruction Kit Part Number

Pumping systems 79852-90008

Variable wavelength detector 79853-90009

Multiple wavelength detector 79854-90008

Autosampler 79855-90008

Diode array detector G1306-90000


4 Installing the Analytical InstrumentsConnecting HP 1050 Series Modules

Cabling

• Connect an GPIB cable between all your HP 1050 modules and the Agilent ChemStation. One GPIB cable is included with the GPIB interface accessory of each HP 1050 module.

The following GPIB cables are available:

• It is recommended that GPIB devices are connected in a daisy-chain configuration in which each device is connected to the previous one using an GPIB cable.

• Avoid using configurations in which all devices are connected to a central point, particularly when cables longer than three meters are used.

Remote Cable

Each analytical component of the system must be connected with an APG remote cable (part number 5061-3378) so that time-critical events, such as injections, can be coordinated. Connect all modules together in a daisy chain.

For further GPIB connection details, see your HP 1050 Series Installing and Maintaining handbook contained in the Instruction Kit included with each module.

Part Number Length

10833A 1 m

10833B 2 m

10833D 0.5 m


Installing the Analytical Instruments 4Connecting HP 1050 Series Modules

Setting the GPIB Addresses

The HP 1050 modules are shipped with the default GPIB addresses as shown in Table 12.

If you configure more than one module of the same type, you must change the default settings so that each module has a unique GPIB address. See the Installing and Maintaining handbooks, included with each analytical module, for information on setting the GPIB address.

Two examples of HP 1050 system configurations are shown in Figure 16 and Figure 17.

Table 12 HP 1050 Default GPIB Addresses

HP 1050 Module GPIB Address

Variable wavelength detector (VWD) 10

Isocratic and quaternary pumps (PMP) 16

Multiple wavelength detector (MWD) 17

Autosampler (ALS) 18

Diode array detector (DAD) 17

Figure 16 Agilent ChemStation with HP 1050 Diode Array Detector


4 Installing the Analytical InstrumentsConnecting HP 1050 Series Modules

Figure 17 HP 1050 System

1. HP 1050 Pumping System2. 10833B GPIB Cable (2 m)3. Agilent ChemStation4. Printer5. Centronics cable6. 10833A GPIB Cable (1 m) included with VWD7. 10833D GPIB Cable (0.5 m) included with Autosampler8. 10833B GPIB Cable (2 m) included with Dual-Channel Interface9. Two signal cables (option to Agilent 35900E)10. Agilent 35900E Dual-Channel Interface11. Remote Cable (5061-3378) included with each HP 1050 module12. HP 1050 Autosampler13. HP 1050 VWD


Installing the Analytical Instruments 4Connecting Miscellaneous Instruments to the Agilent ChemStation

Connecting Miscellaneous Instruments to the Agilent ChemStation

In addition to the HP 1050 Series, HP 1090 and Agilent 1100 Series, you can connect the following stand-alone analytical instruments to your Agilent ChemStation:


• HP 1046 fluorescence detector

• Agilent 35900 dual channel interface

• HP 1049 electrochemical detector

To install these instruments, see the operator’s handbook shipped with each instrument.

The HP 1047A refractive index detector cannot be connected via GPIB. You need a 35900 dual channel interface to acquire data from the HP 1047A.

Setting the GPIB Addresses

These instruments are shipped with the following default GPIB addresses shown in Table 13.

Table 13 Miscellaneous Instrument Default GPIB Addresses

LC Module Default GPIB Address

HP 1040A DAD 15

HP 1046A FLD 12

HP 1049A ECD 11

Agilent 35900E dual channel interface

13


4 Installing the Analytical InstrumentsConnecting Miscellaneous Instruments to the Agilent ChemStation

If you configure more than one module of the same type, you must change the default settings so that each module has a unique GPIB address. For further details, please refer to the instrument handbooks.

Remote Control Cabling

All modules of an instrument that accept timetable entries or which go into the run mode during an analysis must be included in the remote control loop. In general, each module must be connected to the other devices with a remote control cable. The HP 1050 modules, the HP 1046A FLD, the HP 1049A ECD, and the HP 1090 Series II include a dual-APG remote connector with pass-through capability. This means that you may use one remote connector as an input and the other as an output. All remote signals which enter these connectors pass through to the other connector, as well as being seen by the module.

The 5900E dual channel interface use APG remote, but the two remote connectors are not pass-through connections. When you operate the 35900E with both channels, only the A-channel remote will be connected. The B-channel acts in synchronization with the A-channel and will react to the remote signals from the A-channel remote connector. The only time you connect a remote cable to the B-channel remote connector is if you wish to operate the B-channel independently. In this mode, one instrument may start and stop the A-channel while another instrument has control of the B-channel.

The built-in DAD of the HP 1090 Series I LC and the stand-alone HP 1040A DAD do not include standard APG remote connections. It is necessary to connect the remote connection of the HP 1090 mainframe to the remote connector of the built-in or stand-alone DAD, using a single flat-connector remote cable (part number 01040-60202).

When connecting additional devices (that typically support APG-remote) to an HP 1090 system, a signal distribution module must be used.


Installing the Analytical Instruments 4LC Instrument Firmware Requirements

LC Instrument Firmware Requirements

The LC ChemStation software will require minimum firmware revisions in order to work with the following devices shown in Table 14.

Table 14 LC Instrument Firmware Requirements

LC Instrument Product Number Firmware Revision Part Number

HP 1050 isocratic pump 79851A SFW board rev. 3.1 (3027) or higher 01018-66506

HP 1050 quaternary pump 79852A SFW board rev. 3.1 (3027) or higher 01018-66506

HP 1050 VWD 79853A, C GPIB ROM rev. 0.20 79853-13004

HP 1050 VWD 79853A 3.02 79853-13002

HP 1050 VWD 79853C 4.24 79853-13005

HP 1050 VWD 79853C(S/N prefix > 3522)

4.31 79853-13000

HP 1050 MWD 79854A 3.1 (3027) or higher 01048-66504

HP 1050 DAD G1306A 1.0 or higher G1306-69524

HP 1050 autosampler 79855A FIM rev. 4.1 01078-66506

HP 1090L MEM D-3014 01090-66573

HP 1090L LIB E-3049 01090-66579

HP 1090M MCO all revisions 01090-66542

HP 1090M MEM D-3014 01090-66573

HP 1090 II/L LIB E-3049 01090-66579

Agilent 1100 autosampler G1313A A.03.80 or newer n.a.*

Agilent 1100 micro sampler G1389A A.04.10 or newer n.a.†

Agilent 1100 preparative autosampler G2260A A.05.10 or newer n.a.‡

Agilent 1100 well plate autosampler G1367A A.04.13 or newer n.a.**

Agilent 1100 micro well plate autosampler G1377A A.04.13 or newer n.a.††

Agilent 1100 isocratic pump G1310A A.03.80 or newer n.a.*


4 Installing the Analytical InstrumentsLC Instrument Firmware Requirements

Agilent 1100 quaternary pump G1311A A.03.80 or newer n.a.*

Agilent 1100 binary pump G1312A A.03.80 or newer n.a.*

Agilent 1100 capillary pump G1376A A.04.11 or newer n.a.*

Agilent 1100 preparative pump G1361A A.04.17 or newer n.a.*

Agilent 1100 DAD G1315A A.03.80 or newer n.a.*

Agilent 1100 DAD G1315B A.04.11 or newer n.a.*

Agilent 1100 MWD G1365A A.03.80 or newer n.a.*

Agilent 1100 MWD G1365B A.04.11 or newer n.a.*

Agilent 1100 FLD G1321A All Revisions n.a.*

Agilent 1100 VWD G1314A A.03.80 or newer n.a.*

Agilent 1100 RID G1362A A.03.80 or newer n.a.*

Agilent 1100 Fraction Collector G1364A A.05.05 or newer n.a.*

Agilent 1100 Fraction Collector PS G1364B A.05.06 or newer n.a.*

Agilent 1100 Fraction Collector AS G1364C A.05.06 or newer n.a.*

Agilent 1100 Micro Fraction Collector G1364D A.05.06 or newer n.a.*

Agilent 1100 degasser G1322A All Revisions n.a.*

Agilent 1100 local user interface G1323A A.02.01 n.a.*

HP 1040A or HP 1040M DAD COM board firmware rev. E-3014 79880-13007

HP 1040A or HP 1040M DAD DAP board firmware rev. B 79880-69504

dual grating FLD HP 1046A FDC board rev. B-2630 01046-66503

dual grating FLD HP 1046A FDI board with firmware rev. B or C 01046-69509

Electrochemical Detector HP 1049A Firmware rev. E-3314 01049-13002

HP 1090 DAD option #080 COM board firmware rev.E-3014 79880-13007

* The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.

† The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.

‡ The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.

Table 14 LC Instrument Firmware Requirements (continued)

LC Instrument Product Number Firmware Revision Part Number


Installing the Analytical Instruments 4LC Instrument Firmware Requirements

** The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.

†† The Agilent 1100 series of LC modules have flash ROM memory. Firmware updates will be distributed electronically.

NOTE A LAN connection requires the Agilent 1100 modules to have firmware version A.03.80 or higher. The main board (DAM) of the module with the JetDirect card inserted must be GXXXX-66520 (exchange board GXXXX-69520), were XXXX denotes the part number of the Agilent 1100 module.

NOTE If there is a CTL board in the HP 1090, replace it with the assembly kit MCO/MEM board 01090-69708

NOTE Pre-1985 HP 1090 LCs require the following new boards: COM board (79880-69516) and DAP board 79880-69504.

NOTE When connecting an HP 1046A and/or a 35900 A/D dual channel interface to an HP 1090M or HP 1090L (that is, prior to Series II), use the cabling kit 01090-68700 (includes signal distribution module 01090-60302, two remote cables 01040-60202).


4 Installing the Analytical InstrumentsLC Instrument Firmware Requirements



5Checking Out the Agilent ChemStation

Agilent ChemStation Installation Verification 88

Qualifying Operation — Agilent ChemStation Verification Test 94


5 Checking Out the Agilent ChemStationAgilent ChemStation Installation Verification

Agilent ChemStation Installation Verification

Overview

After installing the Agilent ChemStation system software on your computer and configuring the analytical system, your internal validation procedure may require you to assess the correctness and completeness of the installation and to verify that the analytical system is fully operational.

Installation Qualification

The Agilent ChemStation Installation Verification utility uses factory delivered installation reference files to verify the existence, correctness and integrity of the required Agilent ChemStation system files (executable program files, binary register files, macro files, initialization files, help files, customized report templates). This process is called Installation Qualification (IQ).

File integrity is verified by comparing the 16-bit cross-redundancy-check (CRC) checksum of the installed file with the checksum of the original file recorded on Agilent Technologies’s installation master. The file details of the installation master are delivered on so-called reference files. Modified or corrupted files have different checksums and are thus detected by the IQ utility.

The integrity of the reference files themselves is also tracked with the help of checksums. In case the installation verification utility is supplied with a reference file that was modified after its generation, this will be flagged in the report (section invalid reference files).

In addition, the IQ utility checks the version code of the Agilent ChemStation executable system files (*.EXE, *.DLL).


Checking Out the Agilent ChemStation 5Agilent ChemStation Installation Verification

Adding the Agilent ChemStation Installation Verification Utility

The Agilent ChemStation Installation Verification is automatically installed and the appropriate IQ reference files are copied to your PC’s hard-disk and the appropriate Agilent ChemStation IQ icons are added to your Agilent ChemStation group.

Agilent ChemStation Installation Verification Utility Execution

Double-click on the “Installation Qualification” icon in the Agilent ChemStation group.

The checksum calculation for all Agilent ChemStation system files may take several minutes. A dialog box indicates the name of the system file that is currently being analyzed.

NOTE Make sure the Agilent ChemStation is closed before running the Installation Verification Utility.



Agilent ChemStation IQ Utility Results

The Agilent ChemStation Installation Verification utility displays the verification results on the screen and can optionally generate a report of its findings.

On a complete and consistent installation, the installation verification completes without any error messages and no files are reported missing or modified.

If the verification report lists files as missing or modified, the installation verification utility displays the error message Installation Verification completed with error(s) in the message line.

The Agilent ChemStation Installation Verification reports the following file categories:

Table 15 File Categories Reported by Agilent ChemStation Installation Verification

File Category Explanation Required Action

Identical files Required files exist and passed the version and integrity check.

No action required.

Missing files files are missing that are required to run the Agilent ChemStation.

Reinstall the Agilent ChemStation.

Modified files Files have been corrupted or modified.

Reinstall unless you have intentionally customized or updated Agilent ChemStation files; Generate a new installation verification reference file for customized or updated Agilent ChemStation installations.

Invalid reference file The original reference file is corrupt or has been modified after creation.

Reinstall the original reference file



Figure 18 Example for System Modifications Detected by Installation Verification



The printed report contains the license registration numbers used on the system that was checked. It also lists the reference files (name and date code) that were used for the installation verification.

Example report: ChemStation Installation Verification

Installed licensesXM00000000

Reference filessys\lc3d.ref (14. Feb 2001, 12:43)

Identical files(...)hpced02.exe 1.1.5.1 238B HP APG Configuration Editorhpfabs00.dll 1.0.3.3 9BC0 support functions(...)



Generating an Installation Verification Reference File on a Customized Installation

The Agilent ChemStation has an open architecture that allows knowledgeable users to add their own customization code. In regulated environments, it is general practice to document such adaptations, additions or modifications. For your own tracking purposes, you can use the Agilent ChemStation installation verification utility to generate a reference file listing for the customized system. You can use this reference file to qualify the completeness and integrity of the customized Agilent ChemStation and use it as a tracking document for future modifications. The program scans for files with the extensions {*.dlc, *.dll, *.drv, *.enu, *.exe, *.hlp, *.ini, *.mac, *.mcx}.

For example, in order to create the file contents list along with the corresponding file checksums for your existing installation, execute the Agilent installation verification utility with the following command line options:C:\HPCHEM\SYS\HPVERI00.EXE -f custom.ref

Add the resulting reference file to your project documentation. To automatically run an installation qualification test using the new reference file, set up an icon with the following command line specification in its properties:hpveri00.exe -r custom.ref

For a complete description of the command line syntax of the installation verification utility, refer to its help file (“C:\HPCHEM\SYS\HPVERI.HLP”).

NOTE See the installation verification help system for a complete description of the installation verification utility’s command line options. The installation verification does not check method files, sequence files or data files. These files are stored in Agilent Technologies proprietary, unalterable binary formats and are checked for integrity when loaded into the Agilent ChemStation.


5 Checking Out the Agilent ChemStationQualifying Operation — Agilent ChemStation Verification Test

Qualifying Operation — Agilent ChemStation Verification Test

Overview

The Agilent ChemStation provides a verification test function that checks out the system’s software operation. Using either Agilent-supplied or user-generated data files and methods, the system will match calculated integration and quantification results with ones that have been previously generated and archived in an unalterable, checksum-protected, binary register file.

When using an HP 1050 DAD (product number HP G1306A), an Agilent CE instrument with a built-in DAD (product number Agilent G1600A) or an Agilent 1100 Series detector, the verification test can be extended to include a data acquisition check. The verification test generates a report that includes a list of the tested configuration, the verification test details like the data file and method used, and the test results that indicate whether the system passed a specific test step or not.

Performing the Verification Test

• After installing the Agilent ChemStation and successfully passing the Installation Verification, start the Agilent ChemStation

• Select Run Test from the Verification View and run the verification test procedure called default.val.

• Please refer to the analytical tasks section of online help system if you require further information.

Acceptance Criteria

The test was passed successfully, if the verification test report indicates that all individual test steps were passed. This proves that internal components such as the Command Processor, Method Handler, the internal Memory Manager for data objects, registers and tables, the Data Analysis modules for peak identification, peak quantification, report formatting and the Agilent ChemStation print spooler are fully operational.


Checking Out the Agilent ChemStation 5Qualifying Operation — Agilent ChemStation Verification Test

If the test fails, the verification test report indicates which part of the test did not meet the acceptance criteria. Verify you used the correct verification procedure. If the verification test continues to fail, we recommend that you reinstall the Agilent ChemStation. Do not delete your current system installation, as the installation procedure will copy the original Agilent ChemStation files on top of the existing ones.


5 Checking Out the Agilent ChemStationQualifying Operation — Agilent ChemStation Verification Test



6Configuring the Analytical System

Using the Agilent ChemStation Configuration Editor 98

Example for LC Configurations 102

Using the Bootp Protocol 109


6 Configuring the Analytical SystemUsing the Agilent ChemStation Configuration Editor

Using the Agilent ChemStation Configuration Editor

What is the Agilent ChemStation Configuration Editor?

The Agilent ChemStation Configuration Editor is a program that allows the easy configuration of your Agilent ChemStation software. This includes

• Detecting the GPIB interface in your PC

• Selects communication using LAN or GPIB

• Configuring the analytical hardware connected to the PC.

• Configuring the path used for method, data and sequence storage.

• Configuring color display of the Agilent ChemStation.

After installing the Agilent ChemStation software, you are prompted to configure the complete analytical system.

The Agilent ChemStation Configuration Editor needs to be run every time a GPIB device is connected to or disconnected from the GPIB bus of the PC. Since the Configuration Editor modifies the internal communication module database, the Agilent ChemStation must be closed beforehand.

For a LAN connection, the configuration editor only needs to be run, if the IP address of the instrument is changed.


Configuring the Analytical System 6Using the Agilent ChemStation Configuration Editor

Starting the Configuration Editor

Start the Agilent ChemStation Configuration Editor by double-clicking on the Configuration Editor icon in the Agilent ChemStation start-up group or by running “C:\HPCHEM\HPCED02.EXE” from the Start/Run menu.

What You Do

• Select/configure the GPIB interface (for GPIB communication) or setup the bootp server (for LAN communication).

• Configure the instrument type (1090 system, modular system, data analysis only, all with or without spectral capabilities).

• Configure the instrument devices, like special detectors, access points, etc.

• Configure the paths used for methods, sequences and data files (optional).

• Configure the colors used for chromatograms, titles and baselines (optional).

• Save your configuration.

NOTE If GPIB connection is used, a GPIB interface must be installed in the PC, except for the Agilent ChemStation Data Evaluation Only products, Agilent G2090AA and Agilent G2190AA. The I/O Config utility “IOCFG.EXE” must have been run to configure the interface.

NOTE If LAN connection is used, Microsoft TCP/IP protocol must be installed and configured on the Agilent ChemStation PC. In addition a bootp server must be running and configured for the instruments to be used.

CAUTION To avoid communication errors on the GPIB bus, we strongly discourage you from powering modules on or off while the Agilent ChemStation is running.


6 Configuring the Analytical SystemUsing the Agilent ChemStation Configuration Editor

Select and Configure the GPIB Interface

To establish communication through the GPIB bus, the interface configuration must be set in the Configuration Editor. These settings are not necessary if only a LAN connection is used.

We recommend that you check your GPIB card has the correct default settings. See “Installing an GPIB Interface Board in the PC” on page 56 for more information.

1 Choose GPIB Card from the Configure menu. The Configuration Editor attempts to detect the installed card.

2 Either for the Agilent 82341 or the Agilent 82350 GPIB interface, select “hp82341” .

3 Choose OK to save changes and return to the Configuration Editor display.

Configuring Paths

The Configuration Editor lets you specify alternate path settings for your sequences, methods, and data files. This allows storing data files on a separate drive.

NOTE Before you modify the path settings using the Configuration Editor, you need to create the appropriate directories.

CAUTION Do not delete the default method and sequence (“DEF_XX.S” and “DEF_XX.M”, where XX stands for the technique (LC, GC or CE). They are used as a template for new sequences and methods.


Configuring the Analytical System 6Using the Agilent ChemStation Configuration Editor

This section describes how to configure different paths for your methods, sequences, and data files. The Agilent ChemStation default path settings are also listed.

1 Choose Paths from the Configure Menu.

2 Set your data file path. Default = C:\HPCHEM\1\DATA\

3 Set your method path. Default = C:\HPCHEM\1\METHODS\

4 Set your sequence path. Default = C:\HPCHEM\1\SEQUENCE\

5 Choose OK to update the path information and return to the Configuration Editor display.

NOTE All path names have to be terminated with a backslash \.


6 Configuring the Analytical SystemExample for LC Configurations

Example for LC Configurations

Configuring an Agilent ChemStation Instrument

In most cases, the Agilent ChemStation instrument configuration information has to be adapted to match the connected analytical hardware after the installation of the Agilent ChemStation application software. Most Agilent Technologies analytical hardware is modular and can be combined in various ways. The following instrument types are available for an LC configuration:

Table 16 LC Instrument Types in the Agilent Configuration Editor

Instrument Type Supported LC Hardware Data Evaluation Capabilities LAN

1090 3D LC system HP 1090 DAD spectra evaluation included No

1090 LC system HP 1090 no spectral evaluation included No

LC 3D data analysis only no instrument control spectra evaluation included No

LC data analysis only no instrument control no spectra evaluation included No

Modular 3D LC system HP 1050 spectra evaluation included No

Modular LC system HP 1050 no spectra evaluation included No

Modular 3D LC system Agilent 1100 spectra evaluation included Yes

Modular LC system Agilent 1100 no spectra evaluation included Yes


Configuring the Analytical System 6Example for LC Configurations

Configuring an Agilent 1100 Series LC System

The following example describes how to configure an Agilent 1100 Series system with modules connected together by the Agilent 1100 Series controller area network (CAN) that will automatically configure each module.

This section describes how to set up the configuration of your Agilent 1100 Series system.

1 Start the Agilent ChemStation Configuration Editor.

2 Select the Configure/Instruments menu.

3 Select the appropriate instrument type (“Modular LC System” or “Modular 3D LC System”)).

4 Assign a name to your analytical instrument (e.g. Joe’s LC).

5 Select NO for initially start instrument session. This allows you to start your instrument using the appropriate Agilent ChemStation icon.

6 Specify your preferred screen window size (Normal, Icon, Full Screen).

7 Press OK.

8 In the Device Configuration dialog, select “1100 System Access” from the list (see Figure 19 on page 104). In the case of a GPIB connection, select the GPIB radio button and select the GPIB address of the module the GPIB cable is connected to, this is the system access GPIB address. In case of LAN access select the LAN radio button.For the list of default GPIB addresses for Agilent Technologies analytical hardware, press the Help button in the Device Configuration dialog box.

9 Select Add to add the selected system access to the list of configured modules. If LAN access was selected a type in the IP address (or host name if name resolution is implemented on your network) of the Agilent 1100 module with the LAN connection.

10 Select OK to exit and return to the Configuration Editor display.

11 This step is applicable for online instruments using GPIB connection only. For data analysis only configurations or LAN connections, proceed with the next step. Select Configure/GPIB card. The Configuration Editor attempts to detect an GPIB interface in your system. If you obtain a warning message



“No GPIB cards found”, install and/or configure an GPIB interface as described in “GPIB Communication” on page 53.

12 Save your configuration changes by selecting File/Save.

13 This step is applicable for online instruments using LAN connection only. For data analysis only configurations, proceed with the next step. If your IT department did not set up a bootp server for you, you need to do this yourself.

14 When you start the Agilent ChemStation for the first time, the “Configure 1100 System Access” dialog box displays the Agilent 1100 modules detected during instrument initialization (see Figure 20 on page 105). Agilent 1100 modules that have been recognized are listed as “online” (indicated by a green icon) along with their serial numbers. Agilent 1100 modules that have not been detected are marked “offline”. You can selectively take certain modules out of the software configuration, e.g. if your Agilent 1100 system includes two Agilent 1100 detectors and your next analysis only requires one of them. The unneeded Agilent 1100 module may then remain turned off but still be connected. You may also re-order modules, for example if you wish to use one pump as the primary analytical pump and another as a make-up pump. After modifying the configuration, you must restart the Agilent ChemStation. The “Configure 1100 System Access” dialog is available from the Instrument menu in the “Method and Run-Control” view, when Full Menus are displayed.

Figure 19 Agilent ChemStation Configuration Editor: Device Configuration Dialog



Configuring an HP 1050 LC System

You can configure the following modules for use with the Agilent ChemStation:

• HP 1050 Series isocratic pump

• HP 1050 Series quaternary pump

• HP 1050 Series autosampler

• HP 1050 Series diode array detector

• HP 1050 Series multiple wavelength detector

• HP 1050 Series variable wavelength detector

• HP 1046 fluorescence detector

• HP 1049 electrochemical detector

• 35900 dual channel interface

Figure 20 The Configure Agilent 1100 System Access dialog allows to selectively include detected Agilent 1100 modules into the configuration.



This section describes how to configure an HP 1050 Series system with the following devices:

• HP 1050 quaternary pump

• HP 1050 autosampler

• HP 1050 variable wavelength detector

Configuring Your Instrument Devices

This section describes how to set up each device that you have connected to build your instrument.





3 Select the appropriate instrument type (e.g. 1050 3D LC System).




7 Press OK.

8 Choose the HP 1050 Series quaternary pump from the Modules list box.

9 Set the GPIB address in the GPIB address list box. For the HP 1050 quaternary pump the default GPIB address is 16.

10 Choose Add from the main menu to add the HP 1050 quaternary pump in the Selected Modules list box.

11 Choose the HP 1050 autosampler from the Modules list box, set the GPIB address (default is 18) and choose Add to add the HP 1050 autosampler in the Selected Modules list box.

12 Choose the HP 1050 variable wavelength detector from the Modules list box, set the GPIB address (default is 10) and choose Add to add the HP 1050 VWD in the Selected Modules list box.

13 Choose OK to exit and return to the Configuration Editor display.


Saving Configuration Changes

1 Choose File from the Configuration Editor menu.

2 Choose Save from the File menu. Your configuration has now been saved.

NOTE This example assumes an HP 1050 system consisting of a quaternary pump, autosampler and VWD. When you do this yourself, select the modules appropriate to your HP 1050 configuration.

NOTE Choose Help to view a list of the default GPIB addresses, or see Table 12 on page 79.



Configuring an HP 1090 LC System

The following example describes how to configure an HP 1090 system with the following devices:

• HP 1090 PV5 solvent delivery system

• HP 1090 autosampler


Configuring Your Instrument Devices

This section describes how to set up each device that you have connected to build your instrument.



3 Select the appropriate instrument type (e.g. 1090 3D LC System).




7 Press OK.

8 Set the GPIB address of your HP 1090 mainframe (default is 14).

9 Select the HP 1090 diode-array detector from the Modules list box.

10 Set the GPIB address in the GPIB address list box (default is 15).

11 Choose Add from the main menu to add the HP 1090 diode array detector in the Selected Modules list box.

12 Select OK to exit and return to the Configuration Editor display.


NOTE If your HP 1090 uses a filter photometric detector (FPD), you must configure an Agilent 35900C or 35900E dual channel interface to convert the analog detector signals to digital signals for the Agilent ChemStation.


Configuring the Analytical System 6Using the Bootp Protocol

Using the Bootp Protocol

What is the Bootp Protocol

Bootp (Bootstrap Protocol) is used for the central administration and distribution of IP addresses and settings required by devices on a network. For these processes to take place, a Bootp server must be running on the network.

In case of the instrument on the LAN, the HP JetDirect card used to connect the Agilent 1100 modules to the LAN does not store any settings, so each time the instrument is powered on the card sends a request to the network for an IP address and setting information. The implementation of the bootp protocol on the JetDirect card conforms to RFC 951 and RFC 1048.

In order to provide this information to the card, the bootp server must be operational before the card sends the request and the bootp server must be configured to know the hardware address (also called MAC address) of the JetDirect card. Multiple JetDirect cards can be configured with a single bootp server.

If your network is already using a bootp server, please refer to section “Configuring the JetDirect Card Using Bootp” on page 112. If you do not have a bootp server, we suggest to use the CAG bootp server program supplied on the Agilent ChemStation Software family CD-ROM.


6 Configuring the Analytical SystemUsing the Bootp Protocol

Configuring the CAG Bootp Server Program

The CAG bootp server program allows easy configuring of the JetDirect card. Selecting the item Configure -> Add entry allows to easily add a MAC address and IP address pair to the configuration. Using the Configure > Bootp Manager... item, a list of the currently configured MAC address is displayed and can easily be modified.

Getting the MAC Address

The MAC or hardware address of the JetDirect is a world wide unique identifier. No other network device will have the same hardware address. If the MAC address is not printed on the card, perform the following steps

1 Connect the instrument on the LAN to the PC, preferably this should be the only network devices on the network, using the set up described in the section “Single instrument installation” on page 70.

2 Start the bootp server.

3 Open the bootp server window by clicking on it in the taskbar.

4 The window will display the current bootp requests from the total network. If many bootp requests are visible, try to isolate the network segment the PC and the instrument on the LAN are on.



5 Now switch on the instrument on the LAN and view the bootp window. After 2–10 s the bootp server will display the request from the JetDirect card with the hardware address (this information is also stored in the file trace.txt in the bootp server directory, if Log to Disk is enabled from the File menu).

6 Select Configure > Default Settings... and specify the subnet mask and default gateway, in the given example for an isolated connection the subnet mask is 255.255.255.0 (the default gateway setting is not required). Confirm the entry with OK.

7 Select Configure > Add Entry... to add an entry. The drop down box “MAC address” lists all MAC addresses that recently send a bootp request. Select the one for the JetDirect card and make the required settings. Select OK to add the entries.



8 Power cycle the analytical instrument, to force it to send again a bootp request. This time, the MAC address will be found by the bootp server and the CAG Bootp Server window will display the following.

Configuring the JetDirect Card Using Bootp

In order for an internal JetDirect card to use bootp, the default setting: bootp=YES must be set. This is the default setting from the manufacturer and can be viewed on a Agilent 1100 system using the Agilent 1100 control module. In the System view of the control module, select Configure > MIO for the module where the JetDirect card is inserted and scroll down to corresponding item.

To change the setting, the card must be reset; this is done each time the Agilent 1100 module with the JetDirect card inserted is power cycled.

A bootp server uses a file called bootptab were all the necessary information is stored. The bootp server program on the Agilent ChemStation CD-ROM allows easy editing of the bootptab file. If a bootp server is already installed, the following information must be supplied to the system administrator to be able to correctly set up the bootptab file.

NOTE When the MIO dialog is opened on the Agilent 1100 control module, the ChemStation can not communicate with the Agilent 1100 system.



# LC1100 JetDirect Card - Instrument 1LC1100-1:\ht=1:\ha=0060B0047394:\ip=10.10.10.2:\T145=64:T146=01:T147=01

The entries are described below

• # LC1100 JetDirect Card - Instrument 1

Every entry on a line after a # is a comment and ignored by the bootp server.

• LC1100-1:\

Host name of the instrument. The host name communication can only be used if a DNS server (Domain name server) is correctly setup for the name or a corresponding entry in the HOSTS file exists (windows\system32\drivers\etc\hosts).

• ha=0060B0047394:\

This is the hardware or MAC address of the JetDirect card. It is usually printed on a label attached to the card. If not, see section “Getting the MAC Address” on page 110.

• ip=10.10.10.2:\

This is the IP address that will be send to the card. This address needs to be set in the Agilent ChemStation Configuration Editor.

• T145=64:T146=01:T147=01

These parameters set the buffer handling of the JetDirect card. These are required for the instrument on the LAN to work properly and should not be changed.

Additionally entries for the subnet mask and default gateway might be required, depending on your network setup. These values need to be set by your network administrator.



7Troubleshooting

Troubleshooting LAN Communication 116

Troubleshooting GPIB Interface Problems 118

Software Start-up Problems 121

Printing Problems 126

Computer Problems 130

LC Instrument Start-up Problems 132


7 TroubleshootingTroubleshooting LAN Communication

Troubleshooting LAN Communication

Power fail reported on the Agilent ChemStation

If your ChemStation can not connect to the analytical instrument configured using a LAN communication, please perform the following troubleshooting steps.

Verify that the IP Address is correct

Check the IP address and subnet mask of the Agilent 1100 system by using the control module, if available. In the System view of the control module, select Configure->MIO for the module where the JetDirect card is inserted and scroll down to the IP address of the JetDirect card.

Verify that Basic Communication is possible

Use the ping command, to verify that the IP address is operational.

1 Open a command prompt window on your PC.

2 Type “ping 10.10.10.2” and press Enter; where 10.10.10.2 needs to be replaced by the appropriate IP address or by the selected host name.

3 The command ping will send a request for reply to the IP address, bypassing part of the Windows TCP/IP settings. A successful ping would look likeReply from 10.10.10.2: bytes=32 time<10ms TTL=128

If “request timed out” would be displayed, the IP address can not be reached by the ping command.

4 If the ping request was answered successfully by the instrument, you need to verify that your Windows TCP/IP settings are correct for the selected network, especially the subnet mask and gateway settings should be checked.

NOTE When the MIO dialog is opened on the Agilent 1100 control module, the ChemStation can not communicate with the Agilent 1100 system.


Troubleshooting 7Troubleshooting LAN Communication

Identify by host name

If you Identify the Agilent 1100 system by host name, ensure that the used host name and IP address are correctly setup in the DNS server or the corresponding entry in the HOSTS file exists, see “Configuring the JetDirect Card Using Bootp” on page 112. Try using the IP address in the Configuration Editor.

Verify that the JetDirect Card is correctly configured

To ensure that all parameters of the JetDirect card are correctly set, use the CAG Bootp server program from the ChemStation CD-ROM and disable any other means that configure the JetDirect card of the instrument. Install the bootp server as described in section “Installing the CAG Bootp Server Program” on page 52 and configure it for the MAC address of the JetDirect instrument. Leave the bootp server open, power cycle the instrument and verify that the bootp server configures the JetDirect card correctly. Thus you ensure that the parameters for the buffer handling in the JetDirect card are set for analytical instruments.

DHCP Server

Check that no DHCP sever runs in the same subnet as the analytical instruments, as these servers also respond to bootp request and do not set the buffer handling of the JetDirect card correctly. They also may send a different IP address to the instrument each time it is started.

Frequent Buffer Overrun in Instrument Log Book

Please contact your system administrator, to check if the network is not capable of the network traffic induced due to the data acquisition of the instruments. This can also be caused by an incorrectly configured JetDirect card, see above.


7 TroubleshootingTroubleshooting GPIB Interface Problems

Troubleshooting GPIB Interface Problems

The most common problem encountered when using I/O Config is that an interface is not found. For an Agilent 82341 or Agilent 82350 GPIB interface, this is due to one of the following reasons:

• The interface was not installed in the PC before running I/O Config. Install the GPIB interface card in your system and then run I/O Config again.

• The interface is not firmly seated. Ensure that the board is in place and the boards edge connectors ar fully seated. Please refer to the section “Installing an GPIB Interface Board in the PC” on page 56.

• The GPIB interface was replaced by a different type or was installed in a different slot. Rerun I/O Config, remove the configured card and reconfigure the new one.

Figure 21 No GPIB Interface Was Detected on the System

Figure 22 The GPIB Interface Has Already Been Configured.


Troubleshooting 7Troubleshooting GPIB Interface Problems

• The interface is set up to use an I/O port address (Base Address) or interrupt request line (IRQ) that is already being used. To solve this problem, you need to resolve the I/O port address conflict (see “Resolving Agilent 82341 I/O Port Conflicts” on page 119) or IRQ conflict (see “Resolving Agilent 82341 IRQ Conflicts” on page 120). This does only apply to the Agilent 82341 GPIB interface board as the Agilent 82350 is a PCI board.

• The interface has already been configured by I/O Config.To solve this problem, you need to edit the existing configuration entry for the interface, instead of trying to add a new interface. In the main I/O Config window, click on the name of the interface configuration entry for the Agilent 82341 in the Configured Interfaces list box. Then click on the Edit button directly beneath the Configured Interfaces list box to edit the configuration entry for the Agilent 82341 GPIB interface.

Resolving Agilent 82341 I/O Port Conflicts

If I/O Config does not find an Agilent 82341 GPIB interface when you try to add a configuration entry for it, you may have an I/O port (Base Address) conflict with that interface. Neither I/O Config nor the Agilent ChemStation will recognize the Agilent 82341 GPIB interface if a conflict exists with the I/O port Base Address used by the interface. To resolve this problem, select another I/O port address for the interface by changing its DIP switch settings, see Figure 5 on page 54.

Note that the selected I/O port address ranges must not conflict with other I/O interfaces installed in your computer, including other manufacturer's products (such as LAN interfaces, etc.). Refer to the documentation for the other interfaces and the following section to select unique Base Addresses for all the interfaces in your computer.


7 TroubleshootingTroubleshooting GPIB Interface Problems

Resolving Agilent 82341 IRQ Conflicts

The interrupt request line (IRQ) assigned for an Agilent 82341 GPIB interface by I/O Config must be reserved for exclusive use by the interface. If this interrupt line is already being used by another interface, this will cause unpredictable behavior (such as system crashes, LAN problems, mouse tracking problems, etc.).

If you suspect an IRQ conflict exists between an Agilent 82341 interface and another interface on your system, edit the configuration entry for the Agilent 82341 interface and assign another IRQ for the interface.

“Autoadd Button” of IOCFG Installs Defaults Incompatible with the Agilent ChemStation

IOCFG has an “Autoadd” button. We strongly discourage from using the Autoadd button because it installs default settings that are not compatible with the Agilent ChemStation. Please configure the IO Libraries interactively as described in Chapter 5, “Checking Out the Agilent ChemStation”.


Troubleshooting 7Software Start-up Problems

Software Start-up Problems

An online core must be purchased in order to install additional instrument modules

Most probably, a previous version of the Agilent ChemStation was installed on the PC, but the HPCHEM directory subtree was deleted before starting setup. WIN.INI still contains references to the deleted Agilent ChemStation installation in the [PCS] and [PCS,...] sections. Delete the [PCS] sections in WIN.INI.

ChemStation cannot be located on your system. You must first install Agilent ChemStation before installing an instrument

Before installing an add-on instrument control module, you need to install the Agilent ChemStation core (e.g. G2170AA).

ChemStation online session does not finish start-up

No valid license…

Before you can use the Agilent ChemStation, you need to enter the license registration number supplied with the registration packet delivered with your Agilent ChemStation installation disks. You need to enter a registration number for every instrument module that you install.

If you installed the Agilent ChemStation yourself, the installation routine prompts you to enter the license registration number of the appropriate instrument module.

If the Agilent ChemStation core software was preinstalled by Agilent Technologies, you need to install the instrument module software and the license registration number.


7 TroubleshootingSoftware Start-up Problems

If you do not specify the license registration number at time of installation, you need to start the license registration utility from the Agilent ChemStations group of Windows Program Manager. The license registration utility prompts you for the appropriate license registration numbers.

The Agilent ChemStation does not initialize an instrument module for which no valid license registration number has been entered.

Instrument not found

GPIB communication to the configured instrument or instrument module is not possible. This may be caused because:

• the instrument is turned off,

• the instrument’s GPIB address does not match the address configured in the Agilent ChemStation,

• the GPIB cable is not properly connected,

• the GPIB board was not correctly configured (please refer to “Configuring the Agilent 82341 or Agilent 82350 GPIB Interface Drivers” on page 58) or

• the GPIB card is incompatible with your PC.

System Status shows Not Ready

The cause for being in a not-ready state is detected by the instrument. Not-ready conditions of the Agilent liquid chromatographs are described in the documentation accompanying the respective instrument.

Cannot find HPNLS01.DLL

Windows needs this file to run C:\HPCHEM\CORE\HPCORE.EXE

The Agilent ChemStation does not start. You receive error messages that indicate that the Agilent ChemStation initialization could not access specific libraries. You can close all of the error message boxes.

Verify that the Agilent ChemStation main directories (default: C:\HPCHEM and C:\HPCHEM\SYS) are included in the PATH setting of the PC by typing PATH at the MS-DOS prompt. If the Agilent ChemStation system directories are listed in the PATH setting, simply close all applications and restart the computer.



If the directories are not part of the PATH setting, open C:\AUTOEXEC.BAT, find the last entry that specifies “SET PATH” and append “C:\HPCHEM;C:\HPCHEM\SYS” to the line.

…key in section [PCS…] of WIN.INI not valid or specified file does not exist

File Initialization failed

The Agilent ChemStation reports an error during the initialization of its file structure. Verify that the Agilent ChemStation data, method and sequence path settings all point to existing directories on your PC’s hard disk. You can view these settings in the configuration editor.

Go to the [PCS…] section in WIN.INI that was specified in the error message (for example, [PCS,1] and check the contents of the key entry.

If the mismatch is in the _Exe(cution)$ key, check whether the _EXEPATH$ key contains the correct path specification for the Agilent ChemStation core modules (default: C:\HPCHEM\CORE\).

If the _Meth(od)File$ key is reported to be inconsistent, verify that your instrument method directory (for example, C:\HPCHEM\1\METHOD) contains a method directory called def_lc.m.

If the _Seq(uence)File$ key is reported to be inconsistent, verify that your instrument sequence directory (for example, C:\HPCHEM\1\SEQUENCE) contains a sequence file called def_lc.s.

If one or both of them do not exist, either restore a backup copy, copy the corresponding files and directories from another Agilent ChemStation instrument or reinstall the software.

System Exception in dialogs.c

The Agilent ChemStation was terminated abnormally and does not restart. This is usually caused by specific libraries of the application still being active in the PC’s memory. Close down all applications and restart Windows.


7 TroubleshootingSoftware Start-up Problems

Configuration Editor no longer runs

If changes are made to the Windows WIN.INI configuration file manually, or WIN.INI becomes corrupted, it is possible that syntax errors or inconsistencies are introduced into the Agilent ChemStation sections of this file which cause the Configuration Editor to fail.

The best solution to this problem is to restore a backup copy of the WIN.INI file which does not have the errors. If a backup is not available, the configuration sections can be removed manually and the configuration restored using the Configuration Editor. To do this, edit the WIN.INI file using the NOTEPAD editor and remove the sections that are preceded by [PCS,1], [PCS,2], [PCS,3] and [PCS,4], including the section titles. Then, edit the lines for devices and instruments in the main [PCS] section to read:

devices=instruments=

Remove all of the other device statements. At this point, you should be able to run the Configuration Editor. Add the instruments back to the configuration and reconfigure the instrument devices.

If the configuration information is still inconsistent and the configuration editor does not open, we recommend that you remove the [PCS] section from WIN.INI and re-install the software.

Autostart Macro failed

During the initialization, the Agilent ChemStation automatically loads and executes macro code from a defined set of macro files. A run time error caused the autostart macro to fail.

A text error message is displayed in the (red) message line of the Agilent ChemStation.

If you have added your own customization macro code to User.Mac in the Agilent ChemStation core directory (default path: C:\HPCHEM\CORE), check that all the macros loaded in this macro file are correctly specified.

If you cannot identify or isolate the problem, rename User.Mac to Usr.Mac and restart the Agilent ChemStation. If the error message does not occur, you need to debug your customization code.



If the error persists, the working copy of the Agilent ChemStation’s configuration register may have been corrupted. Rename or delete the configuration register in the appropriate instrument directory:

• the configuration register for instrument 1 online is C:\HPCHEM\1\CONFIG.REG, or

• the configuration register for instrument 1 off-line is C:\HPCHEM\1\CONF_OFF.REG.

Alternatively, one of the Agilent ChemStation’s macro files may have been modified or corrupted. In this case, you need to reinstall the Agilent ChemStation software.

General Protection Fault in Module...

The operating system reports a General Protection Fault (GPF) when it detects that an application has attempted write access to a memory location that belongs to another application or process. Potentially, a GPF is the consequence of a system corruption. For the diagnosis of GPFs, it is mandatory to record the exact error message and error address information in order to identify the part of an application program that caused the failure.

Windows allows tracking of GPFs with a utility called Dr. Watson. For more information on this utility, please refer to “Using the DrWatson Utility” on page 143.


7 TroubleshootingPrinting Problems

Printing Problems

ChemStation Print Spooler hangs after an error occurred

If the Agilent ChemStation spooler does not continue after a print error has occurred, try initializing the Agilent ChemStation spooler by typing the following command on the Agilent ChemStation command line:_LoadServiceResetPrinting

the Agilent ChemStation spooler prompts you whether to cancel all pending print jobs. If you do not want to cancel the pending jobs, press No. If the printing error comes again, you need to save your work, close all applications and restart Windows and the Agilent ChemStation to reinitialize the operating environment.

Printing in Multi-Instrument configurations

When printing simultaneously from multiple Agilent ChemStation instruments (e.g during a sequence), resource conflicts may result in printing errors.

If you experience printing problems that appear to be related to a temporary shortage of available system resources as multiple programs print in parallel, you can reduce the Agilent ChemStation’s frequency to hand back CPU control to another application by typing the following command on the Agilent ChemStation command line:_LoadServiceChromSplYield 2000

This command specifies how often (in milliseconds) the Agilent ChemStation Spooler lets other application use the computer’s CPU. The default value is 300 ms. Increasing this number increases the printing speed at the expense of user interface response and should only be used in automated mode.

You can make this setting permanent by adding it to a macro file called USER.MAC in the Agilent ChemStation core directory (default: C:\HPCHEM\CORE). This executes the command automatically every time the


Troubleshooting 7Printing Problems

Agilent ChemStation is started. For further details on the customization possibilities associated with USER.MAC, please refer to the Macro Programming Guide which is available as online help.

Recovering from Printer hang-ups

If for some reason the communication to the printer hangs and the printer therefore does not finish the current printout, there are two steps that have to be done:

Depending on whether the printer is local at your PC or connected via a network:

• on a local printer do a reset on the printer panel itself, or

• on a networked printer you have to clear the network communication problem. This may involve checking cabling, or checking the printer spooler on the network host machine, or doing a reset on the printer panel itself.

On your PC the printer driver or the Windows Print Manager has to be reset. This is done by one of these alternatives:

• if there is a printer driver dialog box visible that has just a Printing… or similar message in it together with a Cancel button, press that Cancel button, or

• if instead there is the icon of the Windows Print Manager visible, close it, discarding the actual print jobs.

If the problem persists try stopping and restarting the Windows Spooler Service. Select Services from the Windows Control Panel, scroll down the list and highlight the item “Spooler”. Choose “Stop” to stop the service and restart it again selecting “Start”.

When the Agilent ChemStation spooler reports printer errors, a message window displays the error messages, for example:Printing problem 106, with page file:c:\hpchem\1\temp\~p3d0004.tmp

These failed print files can be printed with a command typed on the command line, for exampleMFPrint "c:\hpchem\1\temp\~p3d0004.tmp"

Do not forget to delete these temporary files after you have printed them.


7 TroubleshootingPrinting Problems

Printing Messages

Printing Problem 100

A file belonging to the current print job could not be found. Check the consistency of your hard disk’s file structure.

Printing Problems 101, 102, 106, 108, 110, 210, 212, 300

Due to low system resources or not enough available disk space, a print-file could not be accessed either in memory or on disk.

Check free system resources using the Windows Task Manager. Press “Ctrl + Alt + Del” and select “Task Manager”. On the Performance Tab you will find the available physical memory. If free system resources are significantly below 30 %, you should save your work, close all Windows applications and restart Windows.


The print page could not be copied in memory. Check available memory on your computer.


The printer driver could not be initialized.

Reset the Agilent ChemStation spooler and check the printer driver name and version of your printer. Refer to the list of tested printers on the Agilent ChemStation Software CD-ROM.

Check available system resources.


A print page could not be sent to the printer driver.

Verify that your printer is correctly configured and connected and online.

Printing Problems 206, 208, 302

Printer escape sequences to initialize a new page or indicate the end of a print job could not be sent to the printer driver.


Troubleshooting 7Printing Problems

Verify that your printer is correctly configured, connected and online.


A print job could not be removed from the print queue. Either the print job file does not exist in the temporary directory or the Agilent ChemStation print queue file (hpspl100.que) does not exist.

Printing Problem 400, 401, 402, 403

The Agilent ChemStation spooler could not be initialized properly. If this occurs as a consequence of previous errors, save your work, terminate all your applications and restart Windows.

Parts of the chromatogram missing on the report or strange fonts in the report

This may be caused by low system resources in Windows. Either too many applications are running or one or more applications have not freed up all the system resources that they allocated during their operation. Check the percentage free of system resources in the Windows Task Manager. When free system resources get below 30 %, you should save your work, close all applications and restart Windows.

CAUTION The print queue file and Agilent ChemStation temporary files must not be deleted while the Agilent ChemStation is running.


7 TroubleshootingComputer Problems

Computer Problems

Sporadic Hang-Ups

Use CHKDSK (see Chapter 8, “Maintaining the Agilent ChemStation Computer”) to verify that there are no inconsistencies in the file structure. If you find such inconsistencies on your PC’s hard disk, correct them (see “Scanning and Repairing NTFS Volumes” on page 137).

System Crash in an Online Session

If your system crashes when trying to establish the GPIB communication to the chromatograph, there may be hardware conflict between the GPIB board and another device installed in your PC (for example, a specific graphics accelerator card, an infrared port, a soundcard). Using a different IO port and/or a different interrupt level for the GPIB card can eliminate the problem. Refer to the GPIB card configuration information in this handbook.

Cannot create file…

Check the available disk space on your system. Delete unnecessary files, for example, left over temporary files (see Chapter 8, “Maintaining the Agilent ChemStation Computer”) or archive data files that you do not currently need to a backup medium (see “Performing Backups” on page 139).

Slow Hard-Disk access or Hard-Disk activity LED is continuously flickering

Your hard disk may be fragmented. Use a defragmentation utility to reorganize the logical order of file clusters on your disk (see “Defragmenting an NTFS Volume” on page 137). If system performance is generally low and your


Troubleshooting 7Computer Problems

hard-disk appears to be accessed very frequently for relatively long periods of time, the system is most probably low on RAM and using virtual memory (i.e. the page file) excessively.

• Reduce the number of concurrent programs.

• Use Computer Management options (e.g. 2000: right-click on My computer icon and select Manage) to access the disk management utility.

• Check whether your disk cache utility is installed and optimally configured.

Agilent ChemStation performance degrades over time

If your environment requires continuous routine operation of the Agilent ChemStation over days or even weeks without restarting Windows, the system performance may degrade over time due to memory and resource leaks in the system. To overcome this problem, we recommend rebooting the PC on a regular basis.Windows Service Packs are available on the Microsoft Home page.


7 TroubleshootingLC Instrument Start-up Problems

LC Instrument Start-up Problems

Agilent ChemStation does not see an LC hardware modification (HP 1050 or HP 1090)

After modifying the hardware configuration of the LC or an LC module, this change may not be reflected by the Agilent ChemStation.

For example, the Agilent ChemStation does not see the 34-vial tray on the HP 1050 autosampler after the 21 vial tray has been used.

All of these changes require you to close the Agilent ChemStation down and reinitialize it. Subconfiguration information of the LC hardware is only checked at start-up time of the Agilent ChemStation.

Implementing the modification while the Agilent ChemStation is running and simply resetting the module from the Agilent ChemStation user interface is not sufficient.

System remains in a wait-state waiting for a module that has been taken out of the configuration

On systems that are frequently reconfigured, i.e. devices are added or removed from the current configuration, the system may remain in a wait-state as long as the unused devices are still connected to the APG remote cable. This occurs typically when the HP 1040 DAD is removed from the current configuration, but remains connected.

The only possible work-around is to disconnect/connect the remote cable when deleting/adding the HP 1040 DAD from/to the current configuration.

If a second detector is not needed for certain experiments, but for convenience reasons is not taken out of the configuration, selecting a very short run-time for the unneeded detector is not recommended.

For example, when acquiring data on an FLD while the DAD is configured with a short stop-time, it may happen that the DAD lamp is switched off before the LC analysis is finished. This produces a not ready condition so that the next analysis does not start. Alternatively, acquiring with the DAD and selecting a short stop time for the FLD does not produce this problem as a lamp off status is a perfectly valid operational mode with the FLD.


Troubleshooting 7LC Instrument Start-up Problems

Data File is empty (contains no signal)

Check the remote cabling of the detectors. A detector does not change into the run-status unless the start signal is transmitted over the remote line. Refer to the cabling diagrams in “Installing the Analytical Instruments” on page 65.

Devices report “Buffer Overflow” in the logbook

This messages usually indicates by a bad performing PC, when the data transfer from the instrument to the PC is not capable of handling the data stream. The bad performance can be cause by

• Power save features of the PC, refer to section “Advanced Power Management Not Supported with Analytical Hardware” on page 45.

• Bad performing network, refer to section “Frequent Buffer Overrun in Instrument Log Book” on page 117.

• Bad performing hard disk, see section “Slow Hard-Disk access or Hard-Disk activity LED is continuously flickering” on page 130.

• Other programs accessing the hard disk or using the computers resources, like schedule defragmentation, back up or virus scanning.

Agilent 1100 System with Agilent G1315A DAD hangs during initialization

Initialization problems have been reported with some early Agilent 1100 Diode Array Detectors running firmware revision A.02.03 and before. Sporadically, the system would not finish the boot-up sequence, requiring the user to power down the instrument as well as the PC.

To overcome this problem, the Agilent 1100 firmware should be updated.


7 TroubleshootingLC Instrument Start-up Problems



8Maintaining the Agilent ChemStation Computer

Cleaning up Left-Over Temporary Files 136

Maintaining the PC’s File System 137

Performing Backups 139

Gathering System Information 142

Using the DrWatson Utility 143


8 Maintaining the Agilent ChemStation ComputerCleaning up Left-Over Temporary Files

Cleaning up Left-Over Temporary Files

Occasionally, temporary files may accumulate in the directory specified by the TEMP environment variable. These files generally are left open when Windows is abnormally terminated, for example, by powering your computer down without closing Windows first. The Windows temporary files are named ~XXXXXXX.TMP, where XXXXXXX are characters and numbers produced by the program that created the temporary file. To recover the temporary space, you should delete these files after closing all currently running applications.

To determine which directory is currently being used for temporary files, type SET at the command prompt. This will echo back the current settings for all user and system environmental variables.

Notes on the Usage of Long File Names

Current Windows operating systems support long file names, i.e. file names that consist of more characters than in the legacy 8.3 file name convention (8 characters for the prefix and 3 characters for the extension). The Agilent ChemStation currently does not support long file names.

The FAT file system has a hard-coded limit of 512 root level entries and every long file name occupies multiple directory entries. Consequently, the user can run out of directory entries depending on the number and the file name length of entries contained in the root directory of a FAT volume. This is one reason that Agilent recommend the NTFS file system be used for all Agilent ChemStation systems.


Maintaining the Agilent ChemStation Computer 8Maintaining the PC’s File System

Maintaining the PC’s File System

Scanning and Repairing NTFS Volumes

Use the Windows utility CHKDSK from the command prompt to scan and repair NTFS volumes. This program incorporates all of the functionality of the MS DOS-based Chkdsk and Scandisk utilities, including a surface scan. To do a surface scan, use CHKDSK /R. The disk check can also be performed by accessing a disk drive’s properties window and selecting the tools tab.

Windows 2000 and Windows XP Professional perform an autocheck routine upon every boot. If this routine detects a consistency error on the volume, it automatically runs the CHKDSK /F command to fix it. If Windows is unable to run the CHKDSK /F command (for example, because you want to run it on the boot partition or because someone is accessing the partition from the network), this task is postponed until the next time the system reboots.

Defragmenting an NTFS Volume

Compared to the FAT file system, fragmentation of files on an NTFS volume is greatly reduced. By design, fragmentation only occurs on an NTFS volume if a file increases in size after it is on the drive. This means that backing up a volume to tape and restoring it from tape will result in an unfragmented volume.

Windows 2000 Professional and Windows XP Professional does include a simple defragmentation utility. Third-party programs are available that can defragment both Windows 2000 and Windows XP volumes. Agilent recommend Diskeeper from Executive Software (http://www.execsoft.com/).

CAUTION Do not defragement your hard disk drive during data acquisition.


8 Maintaining the Agilent ChemStation ComputerMaintaining the PC’s File System

Scanning for Viruses

If the Agilent ChemStation will communicate with other systems (LAN, modem, BBS, etc.) or if you share files with other computer users, we recommend scanning the drives for computer viruses. Many different utilities are available for virus scanning. Many of these utilities also come with periodic updates as new viruses are discovered.

If you download programs from the internet or exchange documents or programs through e-mail or flexible disks, a virus-check utility is mandatory.

Agilent ChemStation disks (flexible disks, hard-disks and CD-ROMs) are consistently checked for known viruses.

NOTE Never perform a virus scan during data acquisition, as this will degrade the performance of the hard disk and the acquired data might not be written to the hard disk fast enough, causing a power fail or buffer overrun message in the instrument log book and possible loss of data.


Maintaining the Agilent ChemStation Computer 8Performing Backups

Performing Backups

In order to protect your valuable data, an appropriate backup strategy is mandatory. We recommend to archive your data files in regular intervals. If your data is no longer required to be available online, you should also archive them and remove them from your hard disk. This will free up valuable storage space on your computer’s hard disk as data acquired from a diode array detector may easily exceed several megabytes in size.

Windows 2000/XP Backup

Windows 2000 and Windows XP Professional both include compatible backup programs that support many standard tape devices. The backup utility also allows for backing up to a single file, which can then be placed on an offline medium. Both backup utilities allow the Windows Registry to be backed up as well as data.

Automating and Scheduling Windows 2000/XP Backup

Windows 2000/XP allows automation and scheduling of Backup jobs through the use of batch files and the Scheduler service. The Scheduler is available by default for Windows 2000 and Windows XP.

NOTE Never perform a back up during data acquisition, as this will degrade the performance of the hard disk and the acquired data might not be written to the hard disk fast enough, causing a power fail or buffer overrun message in the instrument log book and possible loss of data.


8 Maintaining the Agilent ChemStation ComputerPerforming Backups

Travan Tape Drives

Tape drives are the most common backup devices available today. The IDE tape drives combine lowest initial hardware cost with acceptable performance (up to 4 GB/hour). Travan tapes are a reasonable backup solution for most laboratories that run their Agilent ChemStation on single workstation PCs. The minimum storage capacity per tape is currently around 2.5 GB of uncompressed data.

You will need an appropriate backup utility that supports the drive.

Digital Audio Tape (DAT) Drives

Digital audio tape (DAT) tape drives are suited to handle large amounts of data (storage capacity is typically 2 to 6 GB or more), but do require a SCSI interface installed in the PC.

Magneto-Optical (MO) Disk Drives

MO disk drives are high storage capacity devices (at least 1.3 GB) connected externally to a SCSI interface that must be installed in your PC. MO disk drives are capable of reading and writing write-once media and rewritable disks.

No specific backup software is required. The supporting drivers are shipped with the operating system or the device and allow for accessing the additional MO disk as if it was a floppy disk.

NOTE Never run an MS-DOS-based tape backup utility from Windows. This may result in corrupt file information on the tape.

NOTE If the rewritable disk is formatted with NTFS, it cannot be “hot-swapped”, i.e. ejected and exchanged while the system is running. To change disks, you need to shut down Windows.


Maintaining the Agilent ChemStation Computer 8Performing Backups

Recordable CD-ROM

CD-R (CD-Recordable) systems allow you to record up to 650 MB of data on a single CD-R disk. The CD reader/recorder is usually connected to your PC the IDE interface. The CD-R media have a very long life, making them ideally suited to archiving. The CD-R disks can be read by other computers fitted with CD-ROM drives and appropriate software.

Network Servers

Network servers can also be used for data backup purposes, provided that the network system administrator has implemented an appropriate backup strategy for data stored on the server’s disk. One strategy is to allocate a portion of the server’s hard disk space for your Agilent ChemStation. This portion on the server is called a share which you can use for your standard file operations (access, copy, evaluate files) within Windows.

The advantage of this approach is that backup will be performed centrally by a system administrator for all users that have accounts (logon capabilities) on the system.

CAUTION Never perform a backup with a Windows-based backup utility while the Agilent ChemStation is acquiring data. This may result in inconsistencies in the file structure stored on the backup medium.

NOTE Using a 3.5-inch floppy disk drive is not a reasonable backup solution for the Agilent ChemStation.


8 Maintaining the Agilent ChemStation ComputerGathering System Information

Gathering System Information

Gathering System Information with Windows 2000/XP

Windows 2000 and Windows XP Professional include a comprehensive information reporting and diagnostics program that collects and presents information on device driver information, network usage and system resources such as IRQ, DMA and IO addresses. This utility is called “System Information” and can be found under the System Tools menu in the Windows Accessories menu.

Hardware conflicts will usually also be logged in the Windows Event Viewer, if for instance, Windows cannot start a service due to a misconfigured device.

For Windows XP Professional Error Reporting is implemented and enabled by default.To access the settings for the reporting feature:

1 Click Start

2 Right-click My Computer, then click Properties

3 Click on the Advanced tab

4 Click Error Reporting

For more information, refer to Windows XP Professional help.


Maintaining the Agilent ChemStation Computer 8Using the DrWatson Utility

Using the DrWatson Utility

Who Is Dr. Watson?

Dr. Watson (“DrWatson.EXE”) is a Microsoft diagnostic tool that provides detailed information on the internal state of Windows when General Protection Faults (GPF) occur on the system.

There is a version designed for Windows 3.1, Windows for Workgroups 3.11 and Windows 95 and one for Windows NT. If an application error occurs, Dr. Watson automatically creates a special log file in your WINDOWS directory named DRWATSON.LOG. Dr. Watson also prompts for details about the circumstances (scenario) under which the application error occurred. Sometimes Dr. Watson detects a fault that might not be fatal, and you are given the opportunity to ignore the fault or close the application.

When you choose the Ignore option after an application error is detected, Windows for Workgroups continues without performing the faulting instruction. You might be able to save your work in a new file at this point, but do not continue using the application for other actions. We recommend to quit Windows after saving your work and then restart the PC.

Ignoring the fault may lead to unpredictable results as the first fault already indicated a potentially severe corruption of the system.

When you choose Close, the General Protection Fault dialog box appears, with data that is useful in tracking the source of the error. This information is saved in the DRWATSON.LOG file.

If General Protection Faults reoccur sporadically or even regularly, supply DRWATSON.LOG to your application vendor to discuss the source of the application error.


8 Maintaining the Agilent ChemStation ComputerUsing the DrWatson Utility

Dr. Watson in Windows 2000 /XP

Windows NT, Windows 2000 and Windows XP Professional are coming with two versions of the Dr. Watson utility. There is a 32-bit version of Dr. Watson that traps General Protection Faults caused by 32-bit applications. DRWATSN32.EXE is enabled by default. Errors are logged in a file called DRWATSN32.LOG. More details can be found in the Windows help system.

The second utility is a 16-bit version of Dr. Watson. To install it, create a shortcut to DRWATSON.EXE in the StartUp group. General Protection Faults that occur in 16-bit applications are automatically logged in DRWATSON.LOG.

Unfortunately, this version of DrWatson does not decode segment offsets. This information is useful to the software engineers to identify the source of a failure. For detailed troubleshooting, we recommend replacing current version of DrWatson with version 0.80 that was included with Windows 3.1.

The level of detail of the DrWatson failure reports can be controlled by entries in WIN.INI.

Example:[Dr. Watson]ShowInfo=dis loc mod parTrapZero=1 ; trap floating point exceptionsDisStack=16 ; 16 stack levels to be disassembledDisLen=32 ; 32 instructions disassembled in stack traces

This enables disassembly of the fault address, stack trace disassembly including local variable and parameter values, the list of loaded modules and DLLs and trapping of floating point exceptions.



9Upgrading From a Previous Revision of ChemStation

Introduction 146

Importing Files from a Previous ChemStation Revision 147

Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series) 151

Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series) 153

Upgrading from A.02.0x of the HPLC ChemStation (DOS Series) 154








Importing HPLC ChemStation (Pascal Series) Files 167

Importing Pascal ChemStation files using the LIF Utility 168


9 Upgrading From a Previous Revision of ChemStationIntroduction

Introduction

The installation program allows you to upgrade your system from a previously-installed ChemStation.

The new version installs over an existing installation. Existing sequence, method, data and user files are maintained as your original configuration (instruments, attached devices, addresses).

The ChemStation’s binary files (executable files identified by the extension .exe and .dll) and the ChemStation’s macro files (extension .mac and .mcx) install over the existing files.

To save disk space, the contents of the LCEXE (LC3DEXE, CE3DEXE) directories are deleted during the upgrade.

Files that you added to customize your previous revision of the ChemStation are saved in the ChemStation backup folder (default: C:\HPCHEM\BACKUP). This includes the USER.MAC macro file, if it exists on your system. ChemStation customization code must be reinstalled after an upgrade and checked for compatibility.

The existing icons in the ChemStations group are replaced with the ones pointing to the new installation.

NOTE Co-residence (parallel installations) and co-execution (running concurrently) of the old and new ChemStation revisions are not supported.

NOTE There is no automatic upgrade procedure if your previous ChemStation was the HPLC ChemStation (Pascal Series).


Upgrading From a Previous Revision of ChemStation 9Importing Files from a Previous ChemStation Revision

Importing Files from a Previous ChemStation Revision

The conventions for the sequence and method files have been adapted to the new features that have been implemented since the first introduction. The upgrade path that needs to be followed to convert your analytical sequences and methods depends on the revision of the ChemStation you are upgrading.

In all cases, the raw data file structure has not been changed since the first introduction of the HPLC ChemStation (DOS Series) in 1990, so you can directly load your data.

Importing Sequence Files from Revisions Prior to A.03.01

The sequencing capabilities of this version of the ChemStation include partial sequencing and user-defined data file naming which were not available in the previous revisions. The sequence file structure has changed from a human-readable (and thus modifiable) format to a binary format.

The structure of the sequence tables has been simplified by eliminating redundant tables (the sample log table and the sequence table are now combined into one). This has an impact on the data file naming conventions of sequence data files as the sequence structure information is coded into the data file names of the signals acquired during a sequence.

Reprocessing Sequence Data Imported from a Previous Revision

The steps required to convert a sequence defined with a previous revision of the HPLC2D or HPLC3D ChemStation (DOS Series) are:

• Open the Method&Run Control view.

• Load the appropriate sequence file using the File-Load-Sequence menu. The ChemStation loads the old sequence file and internally flags that the current sequence is an old one.

• Set the Sequence Parameters accordingly (e.g. switch to the appropriate data file directory, switch to Reprocessing Only mode).


9 Upgrading From a Previous Revision of ChemStationImporting Files from a Previous ChemStation Revision

• Start the sequence by specifying Run Sequence from the Method&Run Control Viewer by simply pressing the F6 key.

• Save the sequence using a different name using the File-Save As-Sequence menu.

If you make changes to the sequence table, your sequence is saved according to the new structure. If your original table contains lines that specify runs on a range of vials (e.g. Line 1 run method PEST.M from vial 10 to vial 12 with 2 injections per vial), reprocessing the imported sequence data files does not work. Of course, reacquiring and processing your sequence data works because the sequence data are stored according to the new structure.

The tables below illustrates the difference in sequence processing between the two versions.

NOTE Do not overwrite your original sequence file if you need to be able to rerun the sequence on a previous revision of the ChemStation (e.g. for validation purposes) or need to reprocess sequence data that you imported from a previous ChemStation revision.

Table 17 Old Sequence Table

Sequence line Method From Vial To Vial Injections per Vial

1 Mth1 10 12 2

Table 18 Old Sequence Data File Names

Data File Name Sequence Line Vial Number Injection Number

010-0101.d 1 10 1

010-0102.d 1 10 2

011-0101.d 1 11 1

011-0102.d 1 11 2

012-0101.d 1 12 1

012-0102.d 1 12 2


Upgrading From a Previous Revision of ChemStation 9Importing Files from a Previous ChemStation Revision

Importing Data Files

Data acquired on previous versions of the HPLC ChemStation (DOS Series) can be imported directly (no conversion necessary).

Table 19 New Sequence Data File Names

Data File Name Sequence Line Vial Number Injection Number

010-0101.d 1 10 1

010-0102.d 1 10 2

011-0201.d 2 11 1

011-0202.d 2 11 2

012-0301.d 3 12 1

012-0302.d 3 12 2

Table 20 New Sequence Table (After Conversion to New Sequence Structure)

Sequence Line Method Vial Injections per Vial

1 Mth1 10 2

2 Mth1 11 2

3 Mth1 12 2


9 Upgrading From a Previous Revision of ChemStationImporting Files from a Previous ChemStation Revision

Importing Customized Report Templates

Although the ChemStation now provides a new easy-to-use customized report generator, customized report templates generated with the customized report layout designer of rev. A.01.00 and A.02.0x of the HPLC ChemStation (DOS Series) can still be used.

If you wish to import a user report template created with a previous version of the ChemStation, you need to perform the steps according to the example outlined below.

For example, assume you want to add your customized report template called aa.tpr to the list of available report styles. The title you assign to the customized report is Amino Acids.

1 Copy your user report template (e.g. aa.tpr) to the ChemStation core directory (default: C:\HPCHEM\CORE).

2 On the ChemStation command line, type:AddUserReportStyle “Amino Acids”,”Customize ,””aa””

This adds your template (aa.tpr) to the list of available user report styles. The name of the report style is displayed as Amino Acids. Please note the space after the Customize command and the number of quotes that are necessary for syntactical reasons.

Delete a User Report Style

You can delete a user report style from the list of available report styles.

For example, assume you want to delete the report style named Amino Acids from the list of available reports.

Type the following command on the ChemStation command line:DeleteUserReportStyle “Amino Acids”

This removes a report style entitled Amino Acids from the list of available report styles.

WARNING Modifying imported report templates using the new customized report generator is not possible. To do this, you have to rebuild your report template using the new tool. Please refer to the online help information for more details on how to set up a customized report template.


Upgrading From a Previous Revision of ChemStation 9Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series)

Upgrading from A.01.0x of the HPLC2D ChemStation (DOS Series)

The HPLC2D ChemStation was designed to operate with MS-DOS 3.3 or greater and Windows 3.0.

Importing Method Files

A ChemStation method defines the parameters for instrument control and data acquisition, data analysis and contains recalibration definitions.

In revision A.01.0x of the HPLC2D ChemStation (DOS Series), the data analysis part of the method was implemented as an ChemStation macro. In the later revisions, this was modified so all data analysis parameters were stored in a binary register file.

To import a method generated with revision A.01.0x of the HPLC2D ChemStation (DOS Series), do the following:

1 Switch to the Data Analysis View.

2 Load the default method called def_lc.m by using the File-Load-Method menu. Doing this, a default binary data analysis method register is loaded from disk.

3 Load a data file that was acquired with the method you want to import by using the File-Load Signal menu. This adds the appropriate signal description of the signal to be evaluated by the method to the list of signals.

4 Load the method file that you want to convert using the File-Load Method menu.


9 Upgrading From a Previous Revision of ChemStationUpgrading from A.01.0x of the HPLC2D ChemStation (DOS Series)

5 Save the method under a different name using the File-Save As-Method menu.

Your entire method, including e.g. the calibration table, is now converted to the new revision.

Method Changes since A.01.0x

• A.01.0x includes an older version of the Integrator module. The values accepted for the threshold settings have been redefined. To make the integration threshold settings equivalent on both platforms, the threshold values need to be increased by 5. For instance, if the original A.01.0x method specifies an initial threshold value of -7, you need to modify this setting to -2 in order for the integrator to produce equivalent results.

• The conventions followed by G1304/5A (HPLC2D ChemStation), revision A.01.0x, regarding the origin handling in calibration curve calculations are different from the conventions followed in the later revisions. Revision A.01.0x offers three different modes for origin handling (ignore, include, force origin). The definition of Force Origin has been changed (see below). A new origin handling method Connect Origin has been added, which does exactly what Force Origin did in A.01.0x.

NOTE Do not overwrite your original method if you need to be able to rerun the method using a previous revision of the ChemStation (e.g. for validation purposes).

NOTE According to the old convention, Force Origin calculates the regression curve using the calibration points. The first calibration point is then connected to the origin. This behavior is now called Connect Origin.

NOTE According to the new convention, Force Origin sets the offset term in the equation describing the curve type to zero.

NOTE For exponential and logarithmic curves, the Force Origin cannot be applied as the power curve does not have zero offset.


Upgrading From a Previous Revision of ChemStation 9Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series)

Upgrading from A.01.0 of the HPLC3D ChemStation (DOS Series)


A.01.00 of the HPLC3D ChemStation methods are directly compatible with this version of the ChemStation.

Method Changes since A.01.00

In some cases, Qualifier Evaluation does not work after converting the method to the new revision. This happens only if the original method was saved with the qualifier evaluation flag set to off. As Data Analysis no longer provides a checkbox to switch qualifiers on/off in the calibration settings dialog, the only way to activate the use of qualifiers in the imported method is by typing the following command on the ChemStation’s command line (refer to the Understanding your ChemStation handbook):SetTabHdrVal _DaMethod[1],QuantParm, UseQualifiers, 1


9 Upgrading From a Previous Revision of ChemStationUpgrading from A.02.0x of the HPLC ChemStation (DOS Series)

Upgrading from A.02.0x of the HPLC ChemStation (DOS Series)


Methods generated with this version of the ChemStation are directly compatible.

Method Changes since A.02.0x

Methods now include a dilution factor, in addition to the multiplier that was already part of revision A.02.xx methods.

Column information is restored when the new software is started for the first time. You have to select the currently installed column from the column information screen to make this information available on the reports. Column information is only restored for columns that have been used and identified for the current system. Column information from methods transferred from other systems is not restored.


Upgrading From a Previous Revision of ChemStation 9Upgrading from A.03.0x of the ChemStation

Upgrading from A.03.0x of the ChemStation

A.06.01 of the ChemStation is a major revision, introducing new functionality and a number of defect fixes. Revalidation of the analytical computer system may be required. Refer to the History directory on the Supplemental Disk to help you to determine if you need to revalidate your system.

The following areas have been enhanced in the ChemStation version A.06.01 and have a potential backward compatibility impact on upgraded installations.

Enhanced Integrator

The ChemStation supports the enhanced integrator since revision A:04.01. Using the enhanced integrator is optional. Methods imported from a previous version of the ChemStation can continue to use the original integration algorithm.

The user interface allows the conversion of a method imported from a previous version of the ChemStation. Once a method has been converted to use the new enhanced integration algorithm, it cannot be converted back.

To evaluate the enhanced integration algorithm of the ChemStation, we recommend that you create a backup copy of your original method.

The parameter sets of the original ChemStation integrator and the enhanced integrator are different. For example, parameters such as the initial threshold are values to the power of 2 of a detector specific constant.

The enhanced integrator uses the true physical dimensions for integration parameter settings like slope sensitivity (Response/Time) or height reject (Response).

The results calculated by the two integrators may vary on real chromatograms due to the differences in the determination of baselines, shoulders and tangent skimmed peaks.


9 Upgrading From a Previous Revision of ChemStationUpgrading from A.03.0x of the ChemStation

Internal Storage Format of Quantification Data

Revisions prior to A.04.01 used single precision floating point representation for the internal storage of quantification data; the internal precision of the quantification results used 7 digits.

In revision A.04.01, the internal storage format has been changed to double precision floating point representation, and the internal precision of quantification calculations and results is now 15 digits.

• When comparing ChemStation results to results generated on a previous version of the ChemStation, minor discrepancies may be observed in the last two decimals as rounding effects are now different because of the increased precision of the internal data.

• As a consequence of the increased precision of the internal quantification data, system verification tests generated on a previous version of the ChemStation may now fail due to very minor differences in the number format. If the differences of the system verification test results are caused by the modification of the storage formats, the verification test report states this.

Upgrading from A.03.0x Sequencing User Interface

The modifications introduced in version A.04.01 of the ChemStation are transparent to the user but may have implications for customized systems. The additions and enhancements necessitated to changes to the internal data structures of the sequence engine and the ChemStation method.

Sequences created on previous revisions of the ChemStation can still be loaded and used. Sequences created on version A.04.0x of the ChemStation cannot be used in versions prior to A.04.01.

The following changes have been implemented in the sequencing user interface and internal structure of the ChemStation, version A.04.01:

• The sequence table has been enhanced with an Append Line button.

• The hard-coded link between sample information and specific vials has been removed to allow for easier cut/copy/paste operations on sequence lines.

• The partial sequence screen has been equipped with a print button.



• Better integration of Sequence Summary into the sequence user interface. Sequence Summary Setup is now accessible through a new menu called Sequence Output.

• Sequence recalibration table has been eliminated from the method.

• A new sample type has been implemented to allow for quality control samples. Control samples can be used to verify the system’s suitability to run a defined set of analyses prior to running the real samples. If the defined system suitability criteria are not met, the sequence can be programmed to stop before running the real samples.

Upgrading from A.03.0x methods

The modifications introduced in version A.04.01 of the ChemStation are transparent to the user but may have implications for customized systems. The additions and enhancements necessitated changes to the internal data structures of the sequence engine and the ChemStation method.

Methods created on previous revisions of the ChemStation can still be loaded and used. Methods created on version A.04.0x of the ChemStation cannot be used in versions prior to A.04.01.

• The Sequence Recalibration Table has been removed from the method.

• The data structure of the ChemStation method now has additional fields to accommodate method change history information. When saving modifications to a method, you have the choice to add a modification comment along with the operator name and date to document why the change was made.

• The calibration user interface has been modified. The graphical and logical representation of the calibration table has been rearranged. The logic of the internal data structures has not been modified except for the added support of control samples in the sequence.

• The internal storage format of the quantification data has been changed from single precision to double precision floating point format (see “Internal Storage Format of Quantification Data” on page 156).



Transferring User Report Styles

Customized Report Styles you have added using the Add to Report Styles menu item of the report layout view are normally transferred automatically during the update.

Manually transferring Report Styles

To manually transfer user defined report styles copy your original Repstyle.reg from the old CORE directory (default HPCHEM\CORE) to your current software CORE directory and transfer the report templates (*.frp) from the old REPSTYLE subdirectory.

NOTE This only works if you installed the ChemStation software into the same directory as the previous version and did not remove the HPCHEM directory prior to your update.




A.05.01 of the ChemStation is a major revision, introducing new functionality and a number of defect fixes. Revalidation of the analytical computer system may be required. Refer to the HISTORY directory on the ChemStation CD-ROM to determine if you need to revalidate your system.

The following paragraph outlines the enhancements in revision A.05.01 of the ChemStation that have an impact on backward compatibility.

A.05.0x Default Methods Use the Enhanced Integrator

The default integration algorithm used by revision A.05.01 and later of the ChemStation is the Enhanced Integrator. In previous revisions, the default integration algorithm used was the Standard Integrator. Data analysis methods could be converted to the Enhanced Integrator. See “Enhanced Integrator” on page 155 and the “Understanding Your ChemStation” handbook.

If your environment requires your quantitative results to be backward compatible with results obtained with previous versions of the ChemStation (A.04.02 and earlier), you need to build your data analysis methods using DEFOLDxx.M instead of DEF_xx.M (where xx indicates CE, GC, LC or MS).



A.05.0x Methods May Use Enhanced Peak Purity

The user interface of the Peak Purity function available in the ChemStations for CE, LC and LC/MS has been simplified, but required modifications to the peak purity parameters stored in the data analysis method. If you convert to the Enhanced Peak Purity function, the previous spectra settings are saved in a text file called SPCOPS.OLD in the method directory. Once you have converted a method to use the Enhanced Peak Purity function, you cannot revert back to the original method. Make a backup copy of your original method before converting.

If your environment requires your quantitative results to be backward compatible with the peak purity results obtained with previous versions of the ChemStation (A.04.02 and earlier), you can opt not to use the Enhanced Peak Purity function and continue using the standard one.




A.06.01 of the ChemStation is a major revision, introducing new functionality and a number of defect fixes. Revalidation of the analytical computer system may be required. Refer to the HISTORY directory on the ChemStation software family CD-ROM to determine if you need to revalidate your system.

The following paragraph outlines the enhancements in revision A.06.01 of the ChemStation that have an impact on backward compatibility and may require you to change your documentation and Standard operation procedures (SOP), because they include additional selections in the user interface.

A.06.0x Verification (OQ/PV) methods changed

The methods used for operational qualification and performance verification of the Agilent 1100 system were enhanced. The standard methods can be used by selecting the Standard Tests from the Options menu in the Verification view of the ChemStation. Additional software verification tests were also added that are used by the ChemStation A.06 OQ/PV service (contact Agilent Technologies for details).

Changes in the enhanced integrator

The advanced baseline option was added to the enhanced integrator introduced in revision A.04.01. The tangent skim option was enhanced with respect to A.05.01. The default setting for both options did not change with respect to A.05.01.

Calibration point weights

Calibration points weights 1/Y and 1/Y2 were added to the calibration table.

Report to HTML

The report now also allows to generate HTM files for direct posting on a web server.





The following paragraph outlines the enhancement in revision A.07.01 of the ChemStation that has an impact on backward compatibility and may require you to change your documentation and Standard operation procedures (SOP), because they include additional selections in the user interface.

Improved Sequence Table Function

The sequence filldown utility allows the user to change sequence table column settings for specified vial ranges. Sequence table columns can be selected and values entered for method, sample type, updating response factors and time. Sample name and file name can be given a prefix and autoincremental number.





The following paragraphs outline the enhancements in revision A.08.01 of the ChemStation that have an impact on backward compatibility and may require you to change your documentation and Standard operation procedures (SOP), because they include additional selections in the user interface.

Support of new Agilent 1100 Series Hardware

The ability to control the new Capillary LC system that is designed for increased sensitivity and for analyzing limited sample volumes was added.

The software now supports the Agilent 1100 Series well-plate autosampler, which is available in a standard and thermostatted version.

New ChemStation Plus Security Pack

With revision A.08.0x, you can upgrade the Chemstation product to the Chemstation Plus security pack supporting the FDA requirements of CFR 21 Part 11.




A.09.0x of the ChemStation is a major revision, introducing new functionality and a number of defect fixes. Revalidation of the analytical computer system may be required. Refer to the HISTORY directory on the ChemStation software family CD-ROM to determine if you need to revalidate your system.

The following paragraphs outline the enhancements in revision A.09.0x of the ChemStation that have an impact on backward compatibility and may require you to change your documentation and Standard operation procedures (SOP), because they include additional selections in the user interface.


The ability to control the new Agilent 1100 Series Fraction Collector and the Preparative Scale 1100 Autosampler.

Support for the Agilent 1100 Series Purification System

The A.09.0x ChemStation software supports the recently introduced Agilent 1100 Series Purification System for HPLC and LC/MS.

Support for Microsoft Windows 2000 Professional

With revision A.09.0x, the software is supported on Microsoft Windows 2000 Professional as well as on Windows NT Workstation 4.0.

Support for Version J.02.00 GPIB Drivers

The A.09.0x ChemStation software is supported with the newer J.02.00 GPIB drivers and SICL libraries for the 82350A and 82341C GPIB cards.

No Support for 35900D A/D Card

Due to issues with the 35900D A/D card, which is based on the older ISA bus standard, the A.90.x revision of the ChemStation will no longer support this hardware.





The following paragraphs outline the enhancements in revision A.10.01 of the ChemStation that have an impact on backward compatibility and may require you to change your documentation and Standard operation procedures (SOP), because they include additional selections in the user interface.


The ability to control the new Agilent 1100 Series Fraction Collectors:

The Agilent Chemstation supports the new 40 funnel tray for high volume collection for high flow rates and the new tray for Eppendorf tubes 80.5 ml, 1.5 ml and 2.0 ml).

The A.10.0x ChemStation software supports the recently introduced Agilent 1100 Series Purification System for HPLC and LC/MS. version A02.01.

Support for Microsoft Windows XP Professional

With revision A.10.0x, the software is supported on Windows XP Professional (service pack 1a) as well as on Microsoft Windows 2000 Professional (Service pack 2 and Service pack 3).

Support for Version L.02.01 GPIB Drivers

The A.10.0x ChemStation software is supported with the newer L.02.01 GPIB drivers and SICL libraries for the 82350A/B and 82341C GPIB cards.

G1364B Preparative Scale

G1364C Analytical Scale

G1364D Micro Fraction Collector



XML Interface

The new XML based interface has been implemented to link the Chemstation to LIMS and knowledge management systems.


Upgrading From a Previous Revision of ChemStation 9Importing HPLC ChemStation (Pascal Series) Files

Importing HPLC ChemStation (Pascal Series) Files

Overview

The file system used on the HPLC ChemStation (Pascal Series) is significantly different from the file system available in the MS-DOS environment. The disk drive format used by the HP 9000 Series 310/332/362 Pascal ChemStation systems is called LIF (Logical Interchange Format).

The PC and LIF file systems are not directly compatible. To transfer data between the two, it is necessary to use special conversion utilities.

The LIF utility to convert files between PC and LIF is located in the directory support\lif on the ChemStation software family CD-ROM. To use it, you need 3.5-inch flexible disk drives in your Pascal ChemStation computer and PC.

NOTE The PC must be operating with MS-DOS or Windows 95 since both utilities are incompatible with the Windows NT operating system, neither Windows 2000 or XP.


9 Upgrading From a Previous Revision of ChemStationImporting Pascal ChemStation files using the LIF Utility

Importing Pascal ChemStation files using the LIF Utility

Before You Start

Before getting started with the conversion, do the following:

1 Format a disk on the Pascal system.

2 Install the LIF Utility on your PC by creating a directory called LIF, copy the lifutil.exe file from the disk to this directory. It is most convenient to set up the LIF program as an icon in your ChemStation group, even though it is a non-Windows application.To do this, you need to create a so-called PIF-file for the LIF Utility. PIF stands for program information file. Start the PIF-Editor from the Windows Program Manager Main group and fill in the screen as shown in Figure 23.

NOTE Please note that the HP Copy and LIF Utility programs are not compatible with the Windows NT operating system, Windows 2000 and Windows XP. Any file conversions from the ChemStation (Pascal Series) must be performed on PCs running MS-DOS or Windows 95.


Upgrading From a Previous Revision of ChemStation 9Importing Pascal ChemStation files using the LIF Utility

3 After saving the Program Information File for the LIF Utility, add it as an icon in your ChemStation group to make it the active group, go to the Program Manager screen in Windows, select File, New, Program item. Fill in the screen as shown in Figure 24.

Figure 23 Setting Up the Program Information File for the LIF Utility

Figure 24 Setting up the Program Manager icon for the LIF Utility

NOTE The icon can be changed to the icon shown above by selecting the Change Icon button on the right and selecting from the menu of icons.



Converting ChemStation Data Files using the LIF Utility

1 Copy the Pascal ChemStation data file to the flexible disk using the Pascal ChemStation File Manager utility. For example, use the Copy File(s) softkey. You are prompted for the source and target file names.

2 Place the Pascal disk with the data file(s) in the PC’s flexible disk drive.

3 Start the LIF Utility on your PC.

4 Specify the source drive (LIF drive name) as A: and supply the Pascal ChemStation data file name (LIF file name).

5 Supply the directory for the target file on your PC.

6 Switch the File Name Conversion mode to Extension using the F3 or F4 function keys.

7 In case duplicate file names occur, choose Make unique name in the Duplicate File name settings.

8 Set the file name conversion to Binary Image: no format change.

See the example screen as shown in Figure 25.

Figure 25 Setting up the LIF Utility for a Pascal Data File Conversion



Converting Libraries from the HPLC ChemStation (Pascal Series) to the ChemStation using the LIF Utility

Copying CONVLIB.G to Pascal

1 Insert the Pascal formatted disk into your PC’s flexible drive.

2 Start the LIF program by double clicking on the icon created above.

3 From the main menu choose DOS to LIF <ENTER>. Fill in the LIF screen as shown in Figure 26. With the cursor on the File format conversion field, use the F1/F2 keys to switch conversions choices. After completing the screen, select F5 to start the conversion.

Figure 26 Setup for Copying CONVLIB.G from DOS to PAWS Disk Using the LIF Utility

NOTE Verify that your Pascal system is version 4.1x/5.1x or higher. To determine the version on your system, go to the TOP level and type VER <RETURN>. The current macro for the Pascal part of the conversion only runs on the specified versions.



Converting the Pascal library to .PLF format

1 Take the disk with CONVLIBG to the Pascal system and go to the File Manager screen. First, rename the file from CONVLIBG to CONVLIB.G, then copy CONVLIB.G to the DATA: volume using the copy command.

2 Go to Data Editor and type the following commands:

3 MACRO CONVLIB.G <RETURN>

4 CONVLIB <RETURN>

5 Select the library for conversion from the list. A .PLF file is created using the first 5 characters of the library name. For example, DEMODAD.L becomes DEMOD.PLF.

6 The library conversion process must go through each spectra in the library and the time involved depends on the size of the library. Each entry conversion requires about 20 – 30 seconds. If you have more than one library to convert, do not leave the Pascal ChemStation Data Editor screen. Start the macro again by typing CONVLIB <RETURN>

7 When the conversion to .PLF is complete, return to Top Level.

8 Translate the .PLF files to ASCII format and copy the file to disk as follows:

• Insert the flexible disk in the drive

• Type EX *FILER. <RETURN>(Hint: Do not forget the period after Filer!)

• Type T

• Translate what file? YOUR.PLF <RETURN>

• Translate to what? #3:YOUR.ASC

Converting the .PLF files to ChemStation Library (.UVL) files

1 Place the Pascal disk with the .ASC file(s) in the PC’s flexible drive.

2 Go to the LIF program and select LIF to DOS from the Main Menu. Fill in the screen as shown in Figure 27 on page 173. When the cursor is on the LIF file name field, pressing the F6 key twice lists the contents of the flexible disk. The F4 key can then be used to select the appropriate file. For the file format conversion, again use the F1/F2 keys to switch to the choice shown. In the Copy as field, leave the file name as the system has selected. The file will be renamed later.



3 In the Windows File Manager, select Rename from the File menu to change the name of the file to YOUR.ASC.

4 Go to the ChemStation Spectra Library View and from the File menu select Import Pascal Library.

5 Select the .PLF file from the file selection dialog box, then click the OK button. You are prompted to edit the library header information. If desired, click OK. Each spectra must again be processed so the time for this step depends on the size of your library. It takes about 8 seconds per entry. When the conversion is complete, you are prompted to enter a file name which has a .uvl extension. The default path for spectral libraries is C:\HPCHEM\SPECLIBS.

This completes the conversion process for spectral libraries using the LIF Utility. The .uvl file can now be used independently or incorporated as part of a method for automatic library searches.

Figure 27 Setup for Copying .PLF file from Pascal to DOS Using LIF

NOTE Pascal Method and Sequence files cannot be imported into the ChemStation.



10Supplementary Information

Agilent ChemStation Revision Code Convention 176

Agilent Technologies Response Center Support Services 177

Calling the Agilent Technologies Analytical Response Center 178


10 Supplementary InformationAgilent ChemStation Revision Code Convention

Agilent ChemStation Revision Code Convention

Revision numbers are created according to the scheme outlined below:

P.RR.xx

P- Denotes the identifier of the series of a product. This letter is identical to suffix character in the product number.

Example:

The first A in the product number G2170AA defines that revision code starts with a series identifier A. It is current Agilent Technologies policy not to change the series identifier unless the software changes in purpose or function. The second A in the product number stands for the American English version.

RR- Represents the major revision number. Changes in this code typically includes significant software enhancements that may require complete revalidation of the system for customers operating in regulated environments. These revisions may also include defect fixes and documentation changes.

xx- Represents the minor revision number. These revisions correct software defects and are generally isolated in nature. A minor revision change usually does not require revalidation of the system for customers operating in regulated environments. These revisions only include defect fixes that do not affect the accuracy of the handbooks.


Supplementary Information 10Agilent Technologies Response Center Support Services

Agilent Technologies Response Center Support Services

Agilent Technologies offers different options to help you obtain the professional assistance you need to achieve maximum productivity with your Agilent Technologies analytical software. This service is available worldwide from centers based in Australia, Europe, and the USA.

The Agilent Technologies network of Analytical Response Centers (ARCs) provides access to support professionals who help you resolve operational difficulties, and offer assistance and advice on running Agilent Technologies analytical software. Traditionally, this support is given over the telephone but may also be extended to remote support via modem when you authorize us to do so.

Your Agilent Technologies analytical software includes complimentary Response Center support for a period of 90 days after installation of the product. The duration of the support may be extended to one year or longer at very competitive rates. In addition, you can sign up for a material update service entitling you to automatically receive any software revision upgrades when they are released along with regular delivery of the Software Status Bulletins containing important information on known problems and available work-around solutions for your Agilent Technologies analytical software.

For more information on how to register for these services, please contact your local Analytical Support Representative.

Your local Analytical Support Representative will also provide information on available consulting, customization, development and training services on Agilent Technologies analytical software products.

Agilent Technologies Response-Center support and update services are subject to your local country’s prices, terms and conditions ruling at the time you place your order.


10 Supplementary InformationCalling the Agilent Technologies Analytical Response Center

Calling the Agilent Technologies Analytical Response Center

When you call the Agilent Technologies Analytical Response Center, please be at your computer and have the product documentation at hand.

We recommend that the following information be readily available:

• the registration packet label with the product number, revision code, and license registration number of your analytical software

• the exact wording of any error messages that the system produced

• a list of the instrument modules including firmware revisions that are connected to the PC. You may use the Serialnumber information from the Agilent ChemStation (Method/Run control>Instrument (in Full Menu view only) > Serialnumbers and Columns) to generate this list.

• a printout of the DrWatson.Log file (see “Who Is Dr. Watson?” on page 143)

• a full printout from Windows System Information

• a description of the scenario that leads to the failure.


Index

Aaddress

default GPIB, 81GPIB, 68, 79

AddUserReportStyle, 150advanced power management, 45Agilent 1100 Series, 66, 67

autosampler, 67CAN cabling, 66connecting, 66controller area network (CAN), 103diode-array detector (DAD), 67GPIB cabling, 66pumping systems, 67variable wavelength detector

(VWD), 67Agilent 1100 Series LC

configuring, 103Agilent 1100 system access, 103Agilent 35900 dual channel interface, 81

connecting, 81Agilent 82341, 53, 54, 58Agilent 82341 GP-IB card

switch settings, 55Agilent 82350, 53, 55, 58Agilent ChemStore, 22APM, 45area calculation mode, 154Autoadd Button, 120autosampler

Agilent 1100, 67HP 1050, 77

autostart macro failed, 124

Bbackup

directory, 146strategy, 139

bootp, 52bootp server, 109buffer handling, 113buffer overflow, 133buffer overrun, 117bus address, 64

Ccable

GPIB, 57, 72, 78remote, 66, 78

cabling, 66, 78CAN

Agilent 1100 Series, 66, 103cannot create file, 130cannot find file, 122CD-ROM

recordable, 141changing

area calculation mode, 154checkdisk utility, 130checksum, 88ChemStation

connecting Agilent 1100 Series, 66, 67connecting HP 1050 Series, 77connecting HP 1090 LC, 72connecting miscellaneous

instruments, 81GPIB select code, 107uninstalling, 38

CHKDSK, 130, 137coexecution, 146

COM board, 85commands reference, 20computer problems, 130computer settings, 23config.reg (configuration register), 125configuration

Agilent 1100 Series LC, 103editor, 103instrument devices, 106, 108paths, 100

configuration editor, 99configuration register, 125connecting

printer cable, 44connector

remote, 82controller area network

Agilent 1100 Series, 103Agilent1100 Series, 66

converting Pascal files, 167coresidence, 146CTL board, 85customized report templates

importing, 150

DDAD

Agilent 1100, 67HP 1040, 81HP 1050, 77

DAP board, 85def_lc.m, 100, 123def_lc.s, 100, 123


Index

defaultGPIB addresses, 68, 76, 79, 81method, 100, 123path settings, 101sequence, 100, 123

default GPIB addresses, 68default sequence, 100deleting

user report style, 148detector

Agilent 1100 diode-array, 67Agilent 1100 variable wavelength, 67electrochemical, 81filter photometric, 108HP 1040 diode-array, 81HP 1046 fluorescence, 81HP 1047 refractive index, 81HP 1049 electrochemical, 81HP 1050 diode-array, 77HP 1050 multiple wavelength, 77HP 1050 variable wavelength, 77refractive index, 81

device configuration dialog, 103diagnosing general protection faults, 143diode-array detector, 81

Agilent 1100, 67HP 1040, 81HP 1050, 77

directorybackup, 146

Dr. Watson, 143drwatsn32.exe, 144drwatsn32.log, 144drwatson.exe, 143drwatson.log, 143dual channel, 81dual channel interface, 81

Eelectrochemical detector, 81

HP 1049, 81enhanced

integrator, 155

Ffile initialization failed, 123FILES setting, 128filter photometric detector, 108firmware revisions, 83fluorescence detector, 81

HP 1046, 81

GG1315A DAD, 133GPF, 143GPIB

Agilent 1100 addresses, 68, 71cable, 57, 66, 72, 78connector, 72HP 1050 addresses, 79HP 1090 addresses, 76select code, 107switch settings, 55

GPIB addresses, 68, 79Agilent1100, 68, 71default, 68, 76, 79, 81HP 1050, 79HP 1090, 76

GPIB cables, 72GPIB interface, 42

Hhardware

installing, 42requirements, 45

hardware address, 109, 110host name, 113how-to information, 19HP 1040 diode-array detector, 81

connecting, 81HP 1046 fluorescence detector, 81

connecting, 81HP 1047 refractive index detector, 81HP 1049 electrochemical detector, 81

connecting, 81

HP 1050 Series, 77autosampler (ALS), 77diode-array detector (DAD), 77GPIB cabling, 78multiple wavelength detector

(MWD), 77pumping systems (PMP), 77variable wavelength detector

(VWD), 77HP 1090 liquid chromatograph, 72

connecting, 72GPIB cable, 72

HP 82335, 46, 53hpced02.exe, 99

II/O config utility, 99I/O port conflicts, 119importing

customized report templates, 150Pascal files, 167

initialization problems, 133installation qualification, 88installation verification, 88installing

hardware, 42INSTQ, 89instrument log book

buffer overrun, 117instrument not found, 122instrument types in configuration

editor, 102integrator

enhanced, 155interface, 81

dual channel, 81interrupt line, 64IOCFG, autoadd button, 120iocfg.exe, 99IQ, 88IRQ conflicts, 120


Index

LLAN communication

troubleshooting, 116license registration

utility, 122license registration number, 26license, no valid, 121logical unit, 64

MMAC address, 109, 110, 113macro programming guide, 20magneto-optical disk drives, 140MCO/MEM board, 85method

default, 100, 123upgrading, 157

mobility report, 154multi-instrument configurations

printing, 126multiple wavelength detector

HP 1050, 77MWD

HP 1050, 77

Nnetwork

server, 141network-server based installation, 30non-HP computers, 43not ready state, 122not ready system status, 122NT Workstation, 46

Oonline core, 121operation qualification, 94

Ppath setting, 28

path settingsdefault, 101

PCS section, 123power fail, 116print spooler, 126printing messages, 128printing problem, 128printing problems, 126product structure, 21pumping systems

Agilent 1100, 67HP 1050, 77

Qqualifying

operation, 94quantification data

internal storage format, 156

Rrecordable CD-ROM, 141reference files, 88reference information, 19refractive index detector, 81

HP 1047, 81remote

cable, 66, 78connector, 82

remote cable, 73, 78report

mobility, 154report templates

importing, 150resource conflicts, 44revalidation, 155, 159, 161, 162, 163,

164, 165revision history, 18revision numbers, 176

Sselect code

ChemStation, 107GPIB, 107

sequencedefault, 100, 123upgrading, 156

servernetwork, 141

service pack 3, 46serviceinfo macro, 178show me button, 19SICL interface name, 64signal distribution module, 85software

requirements, 45software certificate and registration

packet, 26software start-up problems, 121specified file does not exist, 123support, 165switch settings

Agilent 82341 GPIB card, 55system controller, 64system exception, 123system status

not ready, 122

Ttask-orientated information, 19TCP/IP, 50test

verification, 94translating Pascal files, 167tutorial, 19

UUNC, 33uninstalling the ChemStation, 38universal naming convention, 33


Index

upgrading, 146methods, 157sequences, 156

user report styledeleting, 148

user.mac, 124

Vvariable wavelength detector

Agilent 1100, 67HP 1050, 77

verificationtest, 94

VISA interface name, 64VWD

Agilent 1100, 67HP 1050, 77

Wwin.ini, 37, 123

update, 107Windows 2000 Professional, 46Windows 95, 46Windows 98, 46write-once media, 140

XXML interface, 166XML support, 37


Agilent Technologies Deutschland GmbH 1994-2003

Printed in Germany 07/03

*G2170-90007**G2170-90007*G2170-90007

www.agilent.com

Agilent Technologies

In This Book

Use this handbook when you install the Agilent ChemStation for the first time or when you make changes to an installed system. This handbook describes how to install the initial software, how to add further instrument modules, how to configure your analytical system, and how to verify that the installation and configuration are complete and operational.

This handbook lists the PC hardware (see “Agilent ChemStation PC Hardware Details" on page 42) and software (see “Operating System Requirements" on page 46) requirements that need to be met in order to install and operate the Agilent ChemStation successfully and explains PC configuration details that you will find useful when upgrading your computer, optimizing your system, or when trying to solve an installation-related problem. You may skip these chapters if your Agilent ChemStation was fully installed by Agilent Technologies.

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