aggregation-proof your protein sample: optisol
DESCRIPTION
Protein Solubility Profiling and Aggregate Solubilization Adresses the number one cause of protein sample leoss: Protein Aggregation. The OptiSol kit yields optimized solubility conditions. Simply screen many solution compositions under critical sample stress and filter out those that prevent protein aggregation. Alternatively, identify solution parameters that solubilize revcersibly aggregated protein samples. Spurress aggregation for meaningful functinal assay results and better purificaiton behavior.TRANSCRIPT
February 2012© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL
OptiSol Reagent Kit
FASOS Technology
Why use OptiSol?
Ensure maximal protein sample utility
Avoid #1 cause of protein sample loss: protein aggregation
Find formulations that protect target protein
from aggregation solubilize aggregated
protein sample Convert problematic
proteins into ‘well-behaved’ research tools
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 2
OptiSol™ Product Components
Kit box: Reagent Plate (96 formulations) Reaction Plate Filter Plate (30, 200 kDa MWCO) Collection Plate Quick-start guide (brochure) Manual & MSDS
Evaluation tool: Protein Dashboard Password & URL for web-download
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OptiSol Product SpecificationCategory Product Details
Product availability
OptiSol 30 or OptiSol 200
Kit box dimensions
7 x 3 x 4 inches
Kit box weight 14 oz (400 g)
Kit components OptiSol Reagent Plate™ (96 formulations, 160 L each) OptiSol Reaction Plate™OptiSol Filter Plate™ (30 kDa MWCO or 200 kDa MWCO) OptiSol Collection Plate™
Analytical software tool
Protein Dashboard™ is a data evaluation tool that supports the interpretation of results (requires MS Excel)
Sample requirements
Recommended Sample Volume: 1-20 L / well (100 L – 2 L protein solution)Recommended Protein Concentration: 1 mg/mL or less (Protein concentration used and protein detection assay need to be matched)
Use requirements
Assay: User provides protein assay. Assay sensitivity needs to be sufficient to detect less than 1/10 of original protein concentration. Examples: Bradford, UV280, ELISA, WESTERN, etc.Equipment: Centrifuge with swing bucket rotor that accepts SBS-sized plate stack
Application A: Protein solubility profilingB: Solubilization of aggregated protein sample.
Shipping Shipped at ambient temperature
Storage Recommended temperature: Reagent Plate (4˚C), rest of kit: room temperature
Stability Kit components are stable for 6 months after receipt
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OptiSol is based on FASOS Technology
FASOS = Filter Aided Solubility Optimization Screening 2 OptiSol Applications
Patent pending
A: Protein Solubility Profiling B: Solubilization of Protein Aggregates
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Sample Stress Examples
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Compatible Analytical Tests
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Simplified Data Evaluation with the Protein Dashboard™
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OptiSol Performance
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OptiSol Product Pricing
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DILYX BIOTECHNOLOGIES, LLC
Feb 2012© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL
Dilyx Biotechnologies, LLC815 First Ave #168
Seattle WA 98104
FAX: 202-207-0398Phone:425-205-0588