ICSU Short Reports A / A ^ T Y N Volume 4

.ADVANCES INGENE TECHNOLOGY:

MOLECULAR BIOLOGYOF THE

ENDOCRINE SYSTEM

Proceedings of the Eighteenth

Miami Winter Symposium [KJ

Miami, Florida, U.S.A.

February 3-7, 1986

Edited by David Puett, Fazal Ahmad,

Sandra Black, Diana M. Lopez, Michael H. Melner,

Walter A. Scott and William J. Whelan

ft VIHmllH.war tm *w*rtMrfW*.

CAMBRIDGE UNIVERSITY PRESS .Cambridge

London New York New RochelieMel bourne Sydney

ContentsPreface xxi

The ICSU Press xxiii

Acknowledgement xxv

SYMPOSIUM PRESENTATIONS

IPEPTIDE AND POLYPEPTIDE HORMONES :

MOLECULAR BIOLOGY AND POST-TRANSLATIONAL PROCESSING OFENDOCRINE HORMONES 2P.C. Andrews, Ourania Andrisani, Tim Hayes, Marie Tavianiniand Jack E. OixonTRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION OFNEUROENDOCRINE GENE EXPRESSION 6Stuart E. Leff, Christian Nelson, E. Bryan Crenshaw, III,Harry Elsholtz, Harry Mangalam, Andrew Russo,Ronald M. Evans and Michael G. Rosenfeld

TISSUE-SPECIFIC HORMONAL REGULATION OF PROOPIONELANOCORTINGENE EXPRESSION 10Micahel H. Melner, Steven L. Young, Frank S. Czerwiec,Deborah Lyn, Arthur E. Jackson, David Puett andRobert D. KoosTRANSCRIPTIONAL REGULATION OF PROOPIOMELANOCORTIN GENEEXPRESSION IN PITUITARY AND BRAIN 14James L. Roberts

LOCALIZATION AND CHARACTERIZATION OF THE HUMAN PLACENTALLACTOGEN GENE ENHANCER 18Bruce L. Rogers, Mark G. Sobnosky and Grady F. Saunders

REGULATION OF PROLACTIN GENE EXPRESSION BY STEROIDS ANDCYCLIC AMP 22Richard A. Maurer

SPECIFIC DNA BINDING PROTEINS AND REGULATION OF TRANSFECTEDPITUITARY GENES 26Carter Bancroft, Bruce A. White, Thomas C. Lufkin andNancy Y. Ip t

CAMP SUBSTITUTES FOR INSULIN IN EXPRESSION OF THE 25KCASEIN GENE IN MAMMARY EXPLANTS FROM VIRGIN RAT 30Piotr Chomczynski

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STUDY OF THE CONTROL OF PRO-THYROID HORMONE (THYROGLOBULIN)GENE EXPRESSION 32D. Christophe, C. Hansen, F. Libert, A. Bacolla, C. Gerardand G. Vassart

DIFFERENTIAL EFFECT OF VARIOUS SECRETOGOGUES ON BETA-ENDORPHIN RELEASE AND PRO-OPIOMELANOCORTIN BIOSYNTHESIS INRAT ANTERIOR PITUITARY CELLS AND AtT-20 CELLS 34Jitendra R. Dave, Lee E. Eiden and Robert L. Eskay

REGULATION OF INSULIN-STIMULATED SUGAR TRANSPORT BYMODULATION OF BASAL SUGAR TRANSPORT 36R.J. Germinario, M. Oliveira and S. Manuel

CHARACTERIZATION OF AN ALPHA-AMIDATING ENZYME FROM RAT CA-77CELLS: ITS USE FOR THE PRODUCTION OF RECOMBINANT HUNANCALCITONIN AND GHRF 38J.P. Gilligan, S.J. Lovato, S.D. Young, B.N. Jones,J.A. Koehn, L.F. LeSueur, A.M. Sturmer, A.H. Bertelsen,T.G. Warren, R.S. Birnbaum and B.A. Roos

REFEEDING INDUCES A GROWTH STIMULATING ACTIVITY IN THEPROXIMAL SMALL INTESTINE IN RATS AFTER 24 HOUR STARVATION 40V.L. Grey and C.L. Morin

CLONING OF A HORMONE-REGULATED ISOFORM OF THE REGULATORYSUBUNIT OF TYPE II cAMP-DEPENDENT PROTEIN KINASE 42T. Jahnsen, L. Hedin, S.M. Lohmann, U. Walter, V.J. Kidd,W.G. Beattie, T.Z. Schulz and J.S. RichardsANTIGEN REGIONS OF BOVINE SOMATOTROPIN DEFINED BY MONOCLONALANTIBODIES 44Gwen G. Krivi and Edwin Rowold, Jr.

MOLECULAR CLONING OF HUMAN ANGIOTENSINOGEN cDNA AND EVIDENCEFOR THE PRESENCE OF TWO mRNAs IN RAT BRAIN 46Satya P. Kunapuli and Ashok Kumar

IMMUNOREACTIVE BETA-ENDORPHIN IN THE GONADOTROPIN-PRIMEDIMMATURE RAT OVARY . 48Edith Lovegren, Stephen Zimniski and David Puett

POSSIBLE ROLE OF INSULIN-LIKE GROWTH FACTORS IN THEREGULATION OF THYROID GROWTH t 50W.W. Mak, B. Bhaumick, R.M. Bala, J.E. Kudlow, M.C. Eggoand G.N. Burrow

AN INSULIN-MEDIATED DIFFERENTIAL INDUCTION OF SPECIFICHEPATIC mRNAs 52K.M. McHugh, C.G. Mucenski and R.L. Drake

RECEPTORS FOR BETA-MSH IN SYNCHRONIZED CLOUOMAN MELANOMA CELLSEXHIBIT POSITIVE COOPERATIVITY IN THE LATE S AND G2 PHASES OFTHE CELL CYCLE 54J. Me Lane and J. Pawelek

PHOSPHORYLATION OF A PROTEIN OF APPROXIMATELY 9OK DALTONS IS AFACTOR IN THE REGULATION OF MELANOMA CELL GROWTH BY INSULINAND THE EXPRESSION OF HIGH AFFINITY RECEPTORS FOR INSULIN 55T. McNeely, A. Slominski, A. Bomirski and J. Pawelek

EFFECTS OF PEPTIOES AND OTHER FACTORS ON IN VITRO GROWTH ANDDIFFERENTIATION OF NORMAL AND MALIGNANT HUMAN COLON CELLS 56H.P. Moyer, P.S. Dixon, D. Escobar, A. Culpepper and J.B. Aust

CLONING OF cDNA FOR A PROLACTIN-INDUCIBLE PROTEIN (PIP) ANDSTUDIES ON THE HORMONAL CONTROL OF PIP GENE EXPRESSION INT47D HUMAN BREAST CANCER CELLS 58Leigh Murphy, Yvonne Myal..Deborah Tsuyuki and Robert Shiu

REGULATION OF ATRIOPEPTIGEN GENE EXPRESSION ATRIUM ANDVENTRICLE OF RAT HEART 60H.E. Tolunay, E.I. Harding, R.C. Wiegand

VARIANT cDNA FOR CANINE PANCREATIC POLYPEPTIDE 62P.J. Toothman and T.L. Paquette

REGULATION OF PROLACTIN PRODUCTION BY PROGESTIN, ESTROGEN ANDRELAXIN IN HUMAN ENDOMETRIAL STROMAL CELLS 64L. Tseng and P. Bischof

ANALYSIS OF THE PROLIFERIN GENE FAMILY IN MOUSE CELLS 66Elizabeth L. Wilder and Daniel I.H. Linzer

GROWTH FACTORS AND ONCOGENESCHANGES IN GENE EXPRESSION FOLLOWING GROWTH STIMULATION OFCULTURED CELLS 70D. Nathans, L.F. Lau, S-J. Lee and D^I.H. Linzer

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EXPRESSION OF GROWTH FACTOR GENES IN TRANSGENIC MICE 74B.L. Davison, L.S. Mathews, G: Norstedt, R.E. Hammer,G.I. Bell, R.L. Brinster and R.D. Palmiter

REGULATION OF INSULIN-LIKE GROWTH FACTOR GENE EXPRESSION 78Mathhew M. Rechler, C.B. Bruni, Y.W.-H. Yang,H.J. Whitfield, D.E. Graham, R. Frunzio,A.L. Brown and S.P. Nissley

REGULATION OF GENE EXPRESSION BY PLATELET-DERIVED GROWTHFACTOR 84Charles D. Stiles

PROTO-ONCOGENE FOS: ROLE IN GROWTH AND DIFFERENTIATION 88Inder M. Verma

REGULATION OF PROTEIN KINASES AND CELL PROLIFERATION BYTRANSFORMING GENE PRODUCTS 92Raymond L. Erikson, J. Blenis and A. Bedard

TYROSINE GLYCOSYLATION IS INVOLVED IN MUSCLE GLYCOGENSYNTHESIS 96Ignacio R. Rodriguez, Juana S. Tandecarz, Brent R. Kirkmanand William J. Whelan

EXPRESSION AND ANALYSIS OF HUMAN TUMOR NECROSIS FACTOR 100S.L. Berent, R.M. Torczynski, N.O. Hill, S. Pichyangkul,F. Jia, J.M. Hill, A. Kahn and A.P. Bollon

EXPRESSION OF c-myc, c-myb AND c-fos oRNA DURING INDUCTIONOF DIFFERENTIATION MYELOMONOCYTIC LEUKENIC CELLS 102F. Bettens, E. Schlick, W.L. Farrar and F.W. Ruscetti

DEVELOPMENT-SPECIFIC EXPRESSION OF INSULIN-LIKE GROWTHFACTOR II mRNA IN DIFFERENT RAT TISSUES 104A.L. Brown, D.E. Graham, S.P. Nissley, D.J. Hill andM.M. Rechler

ACID PHOSPHATASES AS LOCAL MODULATORS OF PROSTATIC CELLGROWTH 106S. Chevalier, C. Bourassa, G. Bleau, K.D. Roberts,A. Chapdelaine

CONSTITUTIVE FORMATION OF MACR0PHAGE-6RANUL0CYTE (GM) ANDMACROPHAGE (M) COLONY STIMULATING FACTORS BY MURINELEUKEMIA X FIBROBLAST HYBRID CELLS 108E.P. Cohen, H.S. Chow and K. Hagen

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HEPARINASE TREATMENT OF ENDOTHELIAL CELLS PREVENTS RESPONSETO HEPATOMA-DERIVED GROWTH FACTOR 110Daniel T. Connolly, Barry L. Stoddard, Mary Beth Knight,Nikos K. Harakas, Joseph Feder and Michael Klagsbrun

PRECISE LOCALIZATION OF THE HU-ETS-2 GENE TO THE CRITICALREGION OF CHROMOSOME 21 IN t8;21 ANL AND DOWN SYNDROME 112H.A. Drabkin, M. Van Keuren, I. Hart, P. Duesberg, N. Sacchi,T.S. Papas and D. Patterson

GENOMIC INSTABILITY ASSOCIATED WITH TUMOR PROGRESSION INHAMSTER BRAIN TUMORS 114G. Duigou, J. Fetherston, J.W. Walsh and S.G. Zimmer

MITOGEN-REGULATED PROTEIN, A MEMBER OF THE PROLACTIN/GROWTHHORMONE FAMILY IS REGULATED LIKE PROLACTIN AT THETRANSCRIPTIONAL AND POST-TRANSLATIONAL LEVELS 116R.T. Hamilton, M. Nil sen-Hamilton, V.R. Fienup and M.-H. Jeng

ISOLATION OF A SYRIAN HAMSTER PLATELET-DERIVED GROWTH FACTORcDNA AND GENOMIC SEQUENCES WHOSE EXPRESSION IS 118REGULATED BY STEROIDSS.E. Harris, J. Hall, M. Harris, R. Monk, J.S. Norrisand R.G. Smith ' -CLONING AND SEQUENCE OF MONKEY MITOCHONDRIAL DNA ORIGIN OFREPLICATION 120E. Karawya and R.G. Martin

HYDROCORTISONE PROMOTES THE NEODIFFERENTIATION OF KIRSTENMURINE SARCOMA VIRUS TRANSFORMED HUMAN SKIN FIBROBLASTSTO ADIPOSE CELLS: RELEVANCE TO ONCOGENIC MECHANISMS 122L. Kopeiovich, R.F. Rich and A.L. Wallace

EXPRESSION OF CELLULAR PROTO-ONCOGENES AFTER IL 2 TREATMENTOF IL 2 INDEPENDENT T CELLS 124E.J. Kovacs, H.A. Young, F.W. Ruscetti, W.L. Farrarand M.C. Birchenall-Sparks

PURIFICATION OF TUMOR INHIBITORY FACTORS FROM COLONCARCINOMA CELLS . 126A.E. Levine, C.A. Crandali, L.C. Yeoman and M.G. Brattain

EXPRESSION OF c-myc, JB, AND JE GENE SEQUENCES ISDIFFERENTIALLY REGULATED IN BALB-3T3, NIH-3T3 AND3T3-ras CELLS 128David H. Lynch and Kathy Kelly

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EFFECT OF INSULIN ON THE GROWTH AND C-MYC ONCOGENEEXPRESSION OF HUMAN HEPATOMA CELLS PLC/PRF/5 130Y. Motoo, M. Mahmoudi, K. Osther and A.P. Bollon

PURIFICATION AND PARTIAL CHARACTERIZATION OFPROSTATE-DERIVED GROWTH FACTOR 132H. Nanri, S. Maehama, D. Li and T.F. Deuel

A CHANGE IN CALCITONIN GENE RNA PROCESSING DURING GROWTHOF HUMAN MEDULLARY THYROID CARCINOMA CELLS IN CULTURE 134B.D. Nelkin, K.Y. Chen, A. de Bustros and S.B. Baylin

LEVELS OF FOS, ETS-2 AND MYB RNAs CORRELATE WITHTUMORIGENICITY OF HeLa/HUMAN FIBROBLAST HYBRIDS 136B.M. O'Hara, H.P. Klinger and D.G. Blair

MAMMARY TUMOR VIRUS RNA EXPRESSION IN SPLENIC BLYMPHOCYTES FROM MMTV-NEGATIVE BALB/C MICE 138Robert J. Pauley and Diana M. Lopez

TSP-180 EXPRESSION AND METASTATIC CAPACITY ON LEWISLUNG CARCINOMA VARIANTS 140A. Sacchi, R. Falcioni, S.J. Kennel, K. Heldin and A. Caputo

ETS GENES IN HUMAN ACUTE LEUKEMIAS 142N. Sacchi, D.K. Watson and T.S. Papas

ENHANCED CASEINKINASE II ACTIVITY DURING MOUSEEMBRYOGENESIS 144H.R. Schneider, G.H. Reichert and O.-G. Issinger

IN VITRO ANALYSIS OF PORCINE MAMMARY-DERIVED GROWTH FACTORS 146F.A. Simmen, K. Cera and D.C. Mahan

EXPRESSION OF A CYTOSKELETON-ASSOCIATED PROTO-ONCOGENEPRODUCT IN ACTIVATED MACROPHAGES 148M.M. Soong, H. Duy, E.S. Didier, S. Fan and W.A.F. Tompkins

SEPARATION OF PROTEIN KINASE ACTIVITIES FROM HDCK ANDDBn 150Joseph A. Syracuse and Mary L. Taub

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TYPE B TRANSFORMING GROWTH FACTORS, EPIDERMALGROWTH FACTOR AND TUMOR PROMOTORS SELECTIVELYINDUCE SECRETED PROTEINS IN MINK LUNG EPITHELIAL CELLS 152Frederic W. Thalacker, Lisa J. Crabill, Scott J. Millerand Marit Nilsen-Hamilton

A NEW MEMBER OF THE TYROSINE KINASE GENE FAMILYEXPRESSED IN A HUMAN T-CELL LEUKEMIA 154J.M. Trevillyan, Y. Lin, S.-J. Chen, C.A. Phillips,C. Canna and T.J. Linna

THE CELLULAR NYC ONCOGENE OF RAINBOW TROUT 156R.J. Van Beneden, D.K. Watson, T.T. Chen and T.S. Papas

THE pl35 TRANSFORMING PROTEIN OF E26 IS NOT CO-TERMINALWITH THE CHICKEN PROTO-ETS GENE PRODUCT 158D.K. Watson, M.J. McWiiliams-Smith, C.S. Flordellisand T.S. Papas

ACUTE LYMPHOBLASTIC LEUKEMIA-ASSOCIATED ANTIGEN, GP24,AND ITS RELATIONSHIP TO PLATELET-DERIVED GROWTH FACTOR 160N.L. Wood, E. Westin and T. Mohanakumar

PEPTIDE AND PROTEIN RECEPTORSTRANSNEMBRANE SIGNALLING BY EGF: EVIDENCE FOR ANALLOSTERIC-OLIGOMERIZATION MECHANISM 164Y. Yarden and J. Schlessinger

THE PDGF RECEPTORS: STRUCTURAL AND FUNCTIONAL STUDIES 168Lewis T. Williams, T.O. Daniel, J.A. Escobedo, V.A. Friedand S.R. Coughlin

ISOLATION, ORGANIZATION, AND EXPRESSION OF THE TRANSFERRINRECEPTOR AND THYNIDINE KINASE GENES 172Frank H. Ruddle

STRUCTURE-FUNCTION RELATIONSHIPS IN THE HUMAN LOW DENSITYLIPOPROTEIN RECEPTOR , 174D.W. Russell, R.W. Bishop, C.G. Davis, M.A. Lehrmanand T. Yamamoto

PURIFICATION OF THE HUMAN PDGF-RECEPTOR: A POLYPEPTIDE WITHINTRINSIC TYROSINE KINASE ACTIVITY 178Subal Bishayee, Alonzo H. Ross, Richard B. Womer andCharles D. Scher

IS COPPER ASSOCIATED WITH ANGIOTENSIN II NUCLEAR RECEPTORS? 180S.E. Bryan, L. LeGros, J. Brown and R.N. Re

EGF RECEPTOR EXPRESSION IN YOUNG AND SENESCENT WI-38 CELLS 182C.R. Carl in and B.B. Knowles

AN ANTIBODY THAT RECOGNIZES AN OPIATE RECEPTORON MURINE SPLENOCYTES 184D.J.J. Carr, K.L. Bost and J.E. Blalock

ENDOTOXIN ALTERS HEPATIC PROLACTIN BINDING IN THELACTATING MOUSE 186Jean K. Carr, Linda M. Keefer and J. Craig Cohen

EXPRESSION OF A HUMAN INSULIN RECEPTOR FROM A CLONEDcDNA IN CHINESE HAMSTER OVARY CELLS 188M. Edery, Y. Ebina, L. Ellis, D. Standring, J. Beaudoin,R.A. Roth and W.J. Rutter

EFFECTS OF MOLYBDATE AND ATP ON THE BINDINGACTIVITY OF THE NUCLEAR T3 RECEPTOR 190R. Faure and J.H. Dussault

IS THE PRIMARY STRUCTURE OF THE INSULIN RECEPTOR TISSUESPECIFIC? 192H.J. Goren, C.R. Kahn, M.F. White and M. Okamoto

ANOMALOUS BINDING OF INSULIN TO RECEPTORS IN LACTATINGRAT MAMMARY GLAND 194M. Holness and H.G. Coore

EXPRESSION OF EPIDERMAL GROWTH FACTOR RECEPTOR IN VARIOUSHUMAN TUMORS 196David L. Hwang, Shu S. Lin, Yee-Chaw Tay and Arye Lev-Ran

EGF-STIMULATED TYROSINE KINASE ACTIVITY IN PLASMAMEMBRANES FROM AS-30D RAT HEPATOMA. THE POSSIBLEROLE OF LONIDAMINE IN INHIBITING GROWTH FACTORSRECEPTORS PHOSPHORYLATION 198M.G. Paggi, A. Floridi, R. Falcioni, C*Nervi,B. Silvestrini and A. Caputo

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CHARACTERIZATION OF THE DETERGENT-SOLUBILIZED RECEPTORFOR ATRIAL NATRIURETIC PEPTIDE FROM CULTURED VASCULARSMOOTH MUSCLE CELLS 200D.B. Schenk, M.N. Phe lps , R. Scarborough, K. Johnsonand J . Lewicki

GLYCOPROTEIN HORMONES

STRUCTURE AND EXPRESSION OF HUMAN PLACENTAL HORMONEGENES 204Irving Boime, Mark Boothby, Susan Daniels-McQueen,Robert Darnell, Makoto Hoshina and Paul Policastro

STRUCTURE AND EXPRESSION OF THE GONADOTROPIN GENES 208J.H. Nilson, R.G. Goodman, J.B. Virgin, M.T. Nejedlik,A. Mi Isted and B.J. Silver

HORMONAL REGULATION OF LUTROPIN AND THYROTROPINGENE EXPRESSION 212William W. Chin, Margaret A. Shupnik, Susan L. Naylor,Soheyla D. Gharib, Frances E. Carr and E. Chester Ridgway

SULFATION OF THE HUMAN CHORIONIC GONADOTROPIN ALPHASUBUNIT IN PLACENTAL EXPLANTS 216M. Bielinska, S. Birken and I. Boime

PROCESSING AND SECRETION OF GLYCOPROTEIN HORMONESUBUNITS IN TRANSFECTED C-127 AND AtT-20 CELLS 218C.L. Corless, T.V. Rambhadran, B.A. Reitz,D.C. Tiemeier and I. Boime

PROTEIN KINASE C ACTIVATORS UNCOUPLEGONADOTROPIN-STINULATED INOSITOL PHOSPHOLIPID HYDROLYSIS INISOLATED BOVINE LUTEAL CELLS 220John S. Davis, W. Allen Conway and Leigh A. West

HUNAN CHORIONIC GONADOTROPIN (hCG) REGULATES LOW DENSITYLIPOPROTEIN RECEPTOR (LDL-R) EXPRESSION IN CULTURED HUMANGRANULOSA CELLS 222T.G. Golos and J.F. Strauss III

CHARGE HETEROGENEITY OF PARTIALLY PURIFIED OVINE LHSUBUNITS AND THE NATIVE HORMONE 224B.A. Keel and H.E. Grotjan, Jr. ,

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DISCORDANT EFFECTS OF TRH ON PRE- AND POST-TRANSLATIONALREGULATION OF TSH BIOSYNTHESIS IN RAT PITUITARY 226S.S. Lippman, S. Amr and B.D. Weintraub

USE OF A CYCLIC AMP ANALOG WHICH INHIBITS CYCLIC AMPDEPENDENT PROTEIN KINASE (A-KINASE) TO STUDY THE ROLEOF CYCLIC AMP AND A-KINASE AS MEDIATORS OF hCG ANDFSH INDUCED STEROIDOGENESIS 228Kelly M. McMasters, Donna M. Anderson, Lynne H. ParkerBotelho, Gordon J. Macdonald and Wm. R. Moyle

EFFECTS OF SWAINSONINE ON PROCESSING AND TURNOVER OFLYSOSOMAL GLYCOPROTEINS 230J.E. Tropea, R.T. Swank and H. L. Segal

STEROID RECEPTORS AND GENE REGULATIONRECEPTORS AND THEIR ACTIONS 234Bert W. O'Malley

PURIFICATION, OLIGOMERISM, REGULATION AND LOCALIZATIONOF THE GLUCOCORTICOID RECEPTOR 238Jan-Aake Gustafsson, 0. Bakke, J. Carlstedt-Duke,K. Fuxe, A. Harfstrand, S. Okret, L. Poellingerand A.-C. Wikstrom

STRUCTURAL ANALYSIS OF HUMAN GLUCOCORTICOID RECEPTOR:DNA BINDING INTERACTIONS 242John A. Cidlowski, Corinne M. Silva, Leslie A. Petch,Douglas B. Tully, Deborah L. Bellingham,Patricia P. Scheible, Mark M. Compton,Victoria E. All good and Christine M. JewellSTEROID HORMONE RECEPTOR GENES: CLONING, ORGANIZATION ANDEXPRESSION 246Pierre Chambon, S. Green, P. Walter, V. Kumar, A. Krust,J.M. Bornert and D. Banner

STEROID HORMONE RECEPTORS AS GENE REGULATORS 250Miguel Beato

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MECHANISMS OF GROWTH REGULATION OF HUMAN BREAST CANCER 254M.E. Lippman, R.B. Dickson, E.P. Gelmann, A. Kasid,S. Bates, C. Knabbe, S.M. Swain, M. McManaway,G. Wilding, N. Davidson, K. Huff and D. Bronzert

LOCALIZATION OF THE GLUCOCORTICOID RECEPTOR INSTEROID-SENSITIVE AND STEROID-RESISTANT HUMAN LEUKEMICCELL LINES 258T. Antakly and E.B. Thompson

EFFECTS OF ESTROGEN ON APOLIPOPROTEIN AI SYNTHESISIN THE HUMAN HEPATOCARCINOMA CELL LINE, HEPG2 260T.K. Archer, S.P. Tarn and R.G. Deeley

PARATHYROID HORMONE REGULATION OF THE RENALPRODUCTION OF 1,25-DIHYDROXYVITAMIN D BYPHOSPHORYLATION AND DEPHOSPHORYLATION 262H. James Armbrecht and Neal A. Siegel

MONOCLONAL ANTIBODY RECOGNITION OF ESTROGEN RECEPTORISOFORMS 264A. Baldi, S.M. Hyder, N. Sato, D.M. Boyle andJ.L. Wittliff

TRANSFECTION STUDIES OF THE PROLACTIN GENE 266E.A. Barron, A.J. Carrillo and Z.D. Sharp

CLONING OF THE 90kDa HEAT-SHOCK PROTEIN INTERACTINGWITH STEROID HORMONE RECEPTORS AND DIFFERENT VIRALONCOGENES 268N. Binart, B. Chambraud, A. Zimiecki, D.M. Helfman,R. W.J. Welsh, J.R. Feramisco, E.E. Baulieu andM.G. Catelli

STUDIES OF NEURONAL TRANSDIFFERENTIATION IN CULTUREDADRENAL CHROMAFFIN CELLS 270J.W. Brown, A. Carballeira, K.F.A. Soliman andL.M. Fishman

IDENTIFICATION OF A NUCLEAR SUBSTRATE OF THE CYCLICAMP-DEPENDENT PROTEIN KINASE OF DICTYOSTELIUM DISCOIDEUM 272T.C. Chambers, J. Song, D.S. Campbell, B.H. Leichtlingand H.V. Rickenberg

DECREASE IN GLUCOCORTICOID RECEPTORS,DURINGCHEMICAL-INDUCED HEPATOCARCINOGENEIS IN RAT 274John Y.H. Chan, Nguyen T. Van and F.F. Becker

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REGULATED EXPRESSION FROM THE PRO-OPIOMELANOCORTINPROMOTER PRESENT ON BOVINE PAPILLOMA VIRUS EPISOMAL DNA 276J. Charron and J. Drouin

ALTERNATIVE 3' ENDS OF HISTONE MRNA 278Nunta Chodcoy, Jane Levine, Art Skoultchi andWilliam F. Marzluff

DNA SEQUENCE ELEMENT RESPONSIBLE FOR INHIBITION OFPRO-OPIOMELANOCORTIN GENE TRANSCRIPTION BY GLUCOCORTICOIDS 280J. Drouin, J.P. Gagner, L. Jeannotte and R.K. Plante

MONOCLONAL ANTIBODIES TO HUMAN LEUKEMIC CELL GLUCOCORTICOIDRECEPTOR 282B. Gametchu and E. Brad Thompson

THE HORMONE-REGULATED rDNA TRANSCRIPTION FACTOR (TFIC)DETERMINES THE EXTENT OF REINITIATION OF TRANSCRIPTIONIN VITRO 284P.K. Gokal, A.H. Cavanaugh, P.B. Mahajan and A.E. Thompson

ESTROGEN REGULATES THE DEGRADATION, NOT THE STABILITY OFAPOLIPOPROTEIN II AND VITELLOGENIN II DRNAS IN CHICK LIVER 286D. Gordon and D.L. Williams

SEQUENCE ORGANIZATION AND SPECIFIC PROTEIN/DNA INTERACTIONSIN REGIONS FLANKING THE ESTROGEN-DEPENDENT AVIAN VERYLOW DENSITY APOLIPOPROTEIN II GENE 288R.J.G. Hache, R.N. Roy, M. Vasa and R.G. Deeley

GENOMIC EFFECTS OF 1,25-DIHYDROXYVITAMIN D 3 UPON THERACHITIC CHICKEN INTESTINE: INDEPENDENCE FROMSERUM CALCIUM STATUS 290A.K. Hall and A.W. Norman

PREDICTING HORMONAL ACTIVITY FROM THE STEREOCHEMISTRY OFNUCLEIC ACIDS: A PROSPECTIVE STUDY WITH NOVEL ESTROGENICSTEROIDS 292L.B. Hendry, T.G. Muldoon, E.D. Bransome, Jr.,A.F. Lehner, M.S. Hutson, N.K. Uberoi, R.B. Myers,A. Segaloff and V.B. Mahesh

HIGH CAPACITY INTRACELLULAR STEROID BINDING PROTEINS 294H. Homma and I. Listowsky

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY PURIFICATION OFTOPOISOMERASE I FROM HUMAN BREAST CANCER CELLS 296Salman M. Hyder, Alberto Baldi and James L. Wittliff

TISSUE-SPECIFIC PROMOTER SEQUENCES IN THEPRO-OPIOMELANOCORTIN GENE 298L. Jeannotte, R.K. Plante and J. Drouin

ANDROGEN INDUCTION OF CDP niRNA, A HUMAN BREAST TUMOR MARKER 300C.A. Kelton and J.E. Monahan

THE MOLECULAR ACTION OF 1,25-DIHYDROXYVITAMIN D 3 IN RATINTESTINE: ISOLATION AND CHARACTERIZATION OF DIFFERENTVITAMIN D 3 -RESPONSIVE cDNA-CLONES 302L. Lamm, M.A. Kessler, H. Darwish and H.F. DeLuca

THE PHYTOESTROGEN COUMESTROL APPEARS CAPABLE OF INSERTIONBETWEEN BASE PAIRS IN DNA IN AGREEMENT WITH ASTEREOCHENICAL RATIONALE FOR HORMONE ACTION 304A.F. Lehner, T.G. Muldoon, V.B. Mahesh,E.D. Bransome, Jr., and L.B. Hendry

STRUCTURE OF THREE MOUSE HISTONE H2A AND H2B GENESINCLUDING ONE PSEUDOGENE ON CHROMOSOME 13 306Ta-Jen Liu, Landie Liu and:Wil1iam F. Marzluff

S.S.S'-L-TRIIODOTHYRONINE (L-T3) BINDING TO RATSSUBCELLULAR FRACTIONS 308A. Llopart-Roman and L.K. Stitzer

EXPRESSION OF Ulb GENES IN MOUSE CELL LINES 310Susan M. Lobo, Naguib Moussa and William F. Marzluff

PROMOTER SEQUENCES INVOLVED IN THE DEVELOPMENTAL REGULATIONOF THE DROSOPHILA MELANOGASTER HSP 23 GENE 312R. Mestril, P. Schiller, J. Amin, H. Klapper,J. Ananthan and R. VoellmyINVESTIGATION OF GLUCOCORTICOID RECEPTOR REGULATION OFAUTOCRINE GROWTH IN A SMOOTH MUSCLE TUMOR CELL LINE 314J.S. Norris, L.E. Cornett, S.E. Harris, A. Nag,P. Rossby, A. Srivastava, S.L. Macleod and R.G. Smith

ISOLATION OF TRANSLATABLE MESSENGER RIBONUCLEIC ACID FORESTROGEN INDUCED UTERINE CREATINE KINASE FROM IMMATURERAT UTERUS 316Ana C. Pastini and J.E. Manjula Devi '

SI

XVI

CLONING OF THE ESTRADIOL INDUCIBLEGLUCOSE-6-PHOSPHATE DEHYDROGENASE GENE OF THE RAT 318K. Yen Pham, C.H. Faust and K.L. Barker

STUDIES OF THE OESTROGEN REGULATION OF CELL-CYCLEREGULATED PROTEINS IN THE MOUSE UTERINE LUMINAL EPITHELIUM 320J.W. Pol lord, S.V.Y. Cheng, B.S. MacDonald andT.R. Cullingford

THYROGLOBULIN mRNA IS REGULATED BY INSULIN AND IGF-1 ASWELL AS BY THYROTROPIN IN THE FRTL-5 THYROID CELL LINE 322P. Santiesteban, L.D. Kohn and R. Di Lauro

POST-TRANSCRIPTIONAL REGULATION OF ALBUMIN GENEEXPRESSION BY ESTROGEN IN XENOPUS LIVER 324D.R. Schoenberg, M.B. Martin and A.T. Riegel

CERVICOVAGINAL (CV) TUMOR LINE LW6214: A POTENTIALMODEL FOR STUDYING THE ETIOLOGICAL EVENTS LEADINGTO ADENO-SQUAMOUS CARCINOMA 326L.A. Scroc.chi and L.A. Jones

GLUCOCORTICOID REGULATION OF RAT INTESTINAL EXTRACELLULARMATRIX AND EPITHELIAL CELL DIFFERENTIATION 328K.M. Sterling, Jr., M.J. Walsh, B. Martin and N.S. LeLeiko

TRANSCRIPTIONAL REGULATION OF THE CALBINDIN-D28K GENE BY1,25-DIHYDROXYVITAMIN D3 IN CHICK INTESTINE 330G. Theofman, A.P. Nguyen and A.W. Norman

ELISA DETECTION OF Z-DNA IN NEGATIVELY SUPERCOILED PLASMIDS 332M.J. Thomas and J.S. Strobl

17 BETA-ESTRADIOL INDUCES PROLACTIN AND GROWTH-RELATEDmRNAS AT DIFFERENT CONCENTRATIONS 334C. Van Itallie and P.S. Dannies

MOLECULAR CLONING OF THE HUMAN GLUCOCORTICOIDRECEPTOR cDNA REVEALS THE CARBOXY-TERMINAL HALF ISRELATED TO THE V-ERBA ONCOGENE PRODUCT 336Cary Weinberger, Stanley M. Hollenberg,Michael G. Rosenfeld and Ronald M. Evans

XV11

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POLYMORPHISM OF STEROID HORMONE RECEPTORS DETECTEDBY HPLC 338J.L. Wittliff, N.A. Shahabi, S.M. Hyder, D.M. Boyle,L. Myatt and L.A. van der Walt

EXPRESSION OF Ul GENES IN SEA URCHIN EMBRYOS 340Jin-Chen Yu, Michael Nash, Carlos Santiagoand William Marzluff

CLONING OF BOVINE ESTROGEN RECEPTOR cDNA 342S. Zain, M. Salim, S. Rehman and A.C. Notides

LYMPHOKINES, INTERLEUKINS,

INTERFERONS AND RECEPTORSTHYNOSINS: COMPONENTS OF BIDIRECTIONAL PATHWAYS BETWEENTHE BRAIN AND IMMUNE SYSTEM 346Nicholas R. Hall and Allan L. Goldstein

THE INTERLEUKIN 2 HORMONE RECEPTOR SYSTEM AND REGULATIONOF T LYMPHOCYTE FUNCTION 350T.R. Malek, J. Miller, R.N. Germain, J.D. Ashwelland E.M. Shevach ;.. .

MOLECULAR ANALYSIS OF THE GENE ENCODING THE HUMANINTERLEUKIN-2 RECEPTOR 354Warren J. Leonard

INTERLEUKIN-2 RECEPTOR ON MALIGNANT CELLS: A TARGETFOR DIAGNOSIS AND THERAPY 358Thomas A. Waldmann

INTERFERON RECEPTOR AND INTERFERON-ACTIVATED GENES 362M. Revel, J. Chebath, A. Kimchi, L. Shulman, 0. Horovitz,B. Cohen, M. Goldberg and M. Fellous

BLOCKING OF FUNCTION OF MOUSE INTERFERON GAMMA BYSYNTHETIC PEPTIDES AND CORRESPONDING EPITOPE-SPECIFICANTISERA 366J.M. Carter, B. Torres, J. Russell and H.M. Johnson

CLONING, SEQUENCE AND EXPRESSION OF BOVINE INTERLEUKIN-2 368D.P. Cerretti, K. McKereghan, A. Larsen,M.A. Cantrell, D. Anderson, S. Gillis, D. Cosmanand P.E. Baker f

xviii

EFFECTS OF INTERFERON-ALPHA ON GROWTH OF A431 CELLS ANDEXPRESSION OF EGF RECEPTORS 370E.H. Chang, T. Masnyk, R. Black and J.B. Harford

THE IFN-INDUCED (2'-5') OLIGO A SYNTHETASE GENE PRODUCESDIFFERENT FORMS OF THE ENZYME 372J. Chebath, P. Benech, L. Mallucci and M. Revel

INCREASED PHOSPHORYLATION OF VIMENTIN IN ACTIVATEDMACROPHAGES 374E.S. Didier, M.M. Soong, S. Fan, and W.A.F. Tompkins

PHOSPHORYLATION OF CYTOSKELETON ASSOCIATED PROTEINS INRESPONSE TO MACROPHAGE ACTIVATION SIGNALS 376S. Fan, M.M. Soong, E.S. Didier and W.A.F. TompkinsB LYMPHOCYTE ENRICHED POPULATIONS PRODUCE IMMUNOREACTIVEENDORPHIN THAT BINDS TO DELTA OPIATE RECEPTORS AND MAYPLAY A ROLE IN ENDOTOXIC SHOCK 378D. Harbour-McMenamin, E.M. Smith and J.E. Blalock

PHOSPHORYLATION OF COMPONENTS OF THE MURINE T CELLANTIGEN RECEPTOR COMPLEX 380J.B. Harford, L.E. Samelson, M.D. Patel, S. Jakenand R.D. Klausner

PURIFICATION TO HOMOGENEITY OF HUNAN INTERLEUKIN-3FROM NORMAL PHA-STIMULATED LEUKOCYTES 382G. Krystal, C.J. Eaves, C. Macdonald and A.C. Eaves

INTERFERON RESPONSIVE AND NON-RESPONSIVE MHC VARIANTSOF A NURINE THYNOMA 384Patrick Machy, Bernt Arnold, Salvador Alino,Barbara Dal ton and Lee D. Leserman

PROPERTIES OF SPECIFIC INTERLEUKIN 1(IL 1) RECEPTORON HUMAN EPSTEIN BARR VIRUS TRANSFORMED B LYMPHOCYTES 386K. Matsushima, T. Akahoshi and J.J. Oppenheim

INTERACTION OF HUMAN INTERFERON-ALPHA WITH CELLULARRECEPTORS - 388L.M. Pfeffer and D.B. Donner

XIX

STRUCTURAL FEATURES OF THE HUMAN INTERLEUKIN-2 RECEPTOR 390D.A. Shackelford and I.S. Trowbridge

INTERLEUKIN-1 INDUCTION OF SYNOVIAL FLUID ANDSYNOVIAL CELL PLASMINOGEN ACTIVATORS 392W. Yang

EXPRESSION OF TRANSFECTED HUMAN GAMMA INTERFERONGENOMIC DNA IN MOUSE FIBROBLASTS 394H.A. Young, L. Varesio and P. Hwu

Author Index 397