advances in biotechnological processes for lactic acid production
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project „Use of integrated bioprocesses in production of lactic acid” (058-0581990-1997, Key Researcher Srđan Novak, Full Professor) supported by Ministry od Science, Education and Sports of the Republic of Croatia. Advances in biotechnological processes for lactic acid production - PowerPoint PPT PresentationTRANSCRIPT
Advances in biotechnological processes for lactic acid production
Anita Slavica, Antonija Trontel, Božidar Šantek, Srđan Novak
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
project „Use of integrated bioprocesses in production of lactic acid” (058-0581990-1997, Key Researcher Srđan Novak, Full Professor)
supported by Ministry od Science, Education and Sports of the Republic of Croatia
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
microorganism
fermentationsimple
carbohydrates LA
fermentation
Industrial bioprocesses for LA production - state of the art
LA - lactic acid
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020 3/21
production of sugar and by-products
investment
land holding and real estate
engineering and production of equipment
production of food and feed products
production of LA and its derivatives
production of energy
Industrial bioprocesses for LA production - state of the art - consolidation of subsidiaries
subsidiary
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Industrial bioprocesses for LA production - world raw sugar market price
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source: FINASUCRE
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Use of produced LA
- traditional use (food industry, pharmaceutical industry,...)
- production of other chemicals and biodegradable polymers (polylactides, PLA) drives global LA demand
LA PLA unit
O-
O
HO
O
O
O
O
n
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
microorganism
fermentationsimple
carbohydrates
renewable raw material
(polysaccharides) LA
simultaneous (semi-)solid substrate saccharification and fermentation to LA
hydrolysis
microorganism
fermentationsimple
carbohydrates LA
fermentation
Advances in industrial bioprocesses for LA production (1) - main principle of new bioprocesses
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Advances in industrial bioprocesses for LA production (2) - microorganism
Lactobacillus amylovorus DSM 20531T - lactic acid bacterium with proteolytic, amylolytic and fermentative activity
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Lactobacillus amylovorus DSM 20531T
MRS medium / 45C / t = 12 h cgs100 / 45C / t = 31 h
cgs100 - corn grits suspension in demineralized waterS0 = 100 g L-1
coloniescells stained by Gram
corn grits particle
the total magnification of microscope 1000X
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Advances in industrial bioprocesses for LA production (2) - microorganism
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Advances in industrial bioprocesses for LA production (3) - bioreactors and media
stirred tank bioreactor horizontal rotating tubular bioreactor
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Advances in industrial bioprocesses for LA production (3) - bioreactors and media
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
media: MRS-starch100, MRS-cg100 and cgs100
medium cgs60
MRS-starch100 and MRS-cg100 - starch and corn grits in MRS medium without glucoseS0 = 100 g L-1
cgs100 and cgs60- corn grits suspension in demineralized waterS0 = 100 and 60 g L-1
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Advances in industrial bioprocesses for LA production (4) - comprehensive analytical toolbox
C6H11O5PO42- + PO4
3- + H+ C2H3OPO42- + C4H7O3PO4
2- + H2O
Methods:
- gravimetric
- spectrophotometric
- chromatographic
- enzymatic
- SDS-PAGE
amino acids, GC
activity of phosphoketolase (EC 4.2.1.22)
t = 0 h t = 2 h t = 6 h t = 72 h t = 216 h
carbohydrates, end products of metabolism, analytical HPLC-IEX
-amylase (EC 3.2.1.1) preparative LC-AF SDS-PAGE
cgs100 / 45C / pH 5.5
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
L. amylovorus DSM 20531T SACCHARIFICATION MRS-starch100 (%) stirred tank bioreactor45C / pH 5.5 / 400 min-1 t = 146,0 h 100
MRS-cg100
stirred tank bioreactor45C / pH 5.5 /400 min-1 t = 153,5 h 100
cgs100 stirred tank bioreactor45C / pH 5.5 / 400 min-1 t = 216,0 h 100 cgs60 horizontal rotating tubular bioreactor 20-35C / pH 4.0-6.2 / 10 min-1
t = 118,0 h (+)
Efficiency of the new bioprocesses for LA production
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
L. amylovorus DSM 20531T SACCHARIFICATION FERMENTATION YLA/S
MRS-starch100 (%) (%) (g g-1) stirred tank bioreactor45C / pH 5.5 / 400 min-1 t = 146,0 h 100 96.3 0.91
MRS-cg100
stirred tank bioreactor45C / pH 5.5 /400 min-1 t = 153,5 h 100 90.7 0.85
cgs100 stirred tank bioreactor45C / pH 5.5 / 400 min-1 t = 216,0 h 100 44.7 0.52 cgs60 horizontal rotating tubular bioreactor 20-35C / pH 4.0-6.2 / 10 min-1 t = 118,0 h (+) (+) (max = 8.90 g L-
1) 13/21
Efficiency of the new bioprocesses for LA production
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
L. amylovorus DSM 20531T SACCHARIFICATION FERMENTATION YLA/S
MRS-starch100 (%) (%) (g g -1) stirred tank bioreactor45C / pH 5.5 / 400 min-1 t = 146,0 h 100 96.3 0.91
MRS-cg100
stirred tank bioreactor45C / pH 5.5 /400 min-1 t = 153,5 h 100 90.7 0.85
cgs100** stirred tank bioreactor45C / pH 5.5 / 400 min-1 t = 216,0 h 100 44.7 0.52 cgs60** horizontal rotating tubular bioreactor 20-35C / pH 4.0-6.2 / 10 min-1
t = 118,0 h (+) (+) (max = 8.90 g L-
1) **(Ala, Gly, Val, Leu, Ile, Pro, Asn, Asp, Met, Glu, Phe, Lys, Trp, Tyr)
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Efficiency of the new bioprocesses for LA production
Bachelor of Biotechnology Master of Bioprocess Engineering or PhD Master of Molecular Biotechnology Martina Andlar Ana Batušić Antonija Trontel Tea Blaško Vanda Baršić Filip Cingesar† Vedrana Buljan Ines Ivanuša Ivana Dusper Dijana Petrić Tomislav Grzunov
Ivana Gusić Nuša Jelovac Željka Kosovec Nikolina Mataga Iva Marković Morana Peklić Jadranka Sučević Vedrana Tadić
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
project „Use of integrated bioprocesses in production of lactic acid” (058-0581990-1997, Key Researcher Srđan Novak, Full Professor)
supported by Ministry od Science, Education and Sports of the Republic of Croatia
Youth on the move
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Where the new bioprocess for LA production is now (1)
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The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Innovative bioprocess was developed √
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Where the new bioprocess for LA production is now (1)
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Innovative bioprocess was developed √
More resource efficient, greener and competitive
bioprocess was developed √
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Where the new bioprocess for LA production is now (2)
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Innovative bioprocess was developed √
More resource efficient, greener and competitive
bioprocess was developed √
The performance of education system was enhanced
and the entry of young people to the labour market was
facilitated (Youth on the move) √
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Where the new bioprocess for LA production is now (3)
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
The project 058-0581990-1997 is following
E U R O P E 2 0 2 0
A European strategy for smart, sustainable and inclusive growth.
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Where the new bioprocess for LA production is now (4)
project „Use of integrated bioprocesses in production of lactic acid” (058-0581990-1997, Key Researcher Srđan Novak, Full Professor)
supported by Ministry od Science, Education and Sports of the Republic of Croatia
The 2nd International Symposium „VERA JOHANIDES“ - BIOTECHNOLOGY IN CROATIA BY 2020
Thank you for your attention!
ContactAnita Slavica, Associate Professor University of Zagreb, Faculty of Food Technology and Biotechnology (http://www.pbf.unizg.hr)Department of Biochemical Engineering Laboratory of Biochemical Engineering, Industrial Microbiology, Malting and Brewing Technology Pierottijeva 6/IV HR-10000 Zagreb Tel.: +385-(0)1-4605-142 Fax.: +385-(0)1-4836-424 e-mail: [email protected]
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