advance protein powder nutritional report

Julie Spero, MBA, NC 1 of 15

Advance Protein Powder Nutritional Report Julie Spero, MBA, NC President, National Association of Nutrition Professionals

18 May 2014

Overview: Advance Protein Powder (APP) is a high quality, organically derived, wild marine-‐based

protein powder, which is highly stable with a shelf life of over 5 years. It is virtually odorless

and tasteless with high levels of naturally occurring organic minerals giving it a nutritional

profile superior to other protein powders on the market. APP is made using a patent-‐

pending manufacturing process that is both green and sustainable. It is non-‐hygroscopic and

very moisture resistant, contributing to its long shelf life. This report presents an overview of

the health benefits of the all natural Advance Protein Powder comparing it with BlueWave,

another fish protein product on the market and also with several examples from the two

most common protein supplement categories available: whey and soy.


About Whey protein powder Whey used to be considered a dairy industry waste product. Whey is what remains after the

butter, cream, milk is drawn off and the cheese has been made. The dairy industry long

considered whey a problem because dumping it into sewer systems or feeding it to pigs cost

as much or slightly more than the value of the whey as a commodity. This problem was

solved when manufacturers developed techniques for drying the whey at high temperatures

and putting the powders into body building powders, high protein bars and other food

products.i

While there are some high quality whey protein powders available, many more are of

marginal or poor quality. The most expensive and better quality products are made from

organic milk and processed at low temperatures to preserve the maximum amino acid

content potential and maintain a full range of important protein components such as

immunoglobulins and serum albumin (which are also present in APP fish protein). The quality

of other whey protein powders can vary widely depending on the source of the milk used as

well as the various steps in processing. Commercial milk often contains growth hormone

(rBGH) and a number of antibiotics; the FDA has approved thirty. An estimated sixty percent

of herbicides used in the U.S. as well as pesticides and other petrochemicals are applied to

produce higher yields of feed crops that are eaten by commercial dairy cows and are present

in the milk they produce. As you can see, the quality of the whey can only be as good as the

quality of the food, nutrition and care given to the cows.

The Whey protein products chosen for this comparison represent products that are available

for purchase in bulk or commonly used in protein drinks and bars. They include an organic

whey protein product from Source Organic who declare their cows have never been given

hormones, antibiotics, steroids, or genetically modified feed (GMO). The others are plain

protein products with no flavoring added. Whey protein (of good quality) is one of the most

bio-‐available sources of supplemental protein available at 96-‐98%. As mentioned, the quality

can vary considerably depending on the milk source and the methods used in processing.


• Source Organic Whey protein is processed using low temperature drying and filtration

and therefore retains immune components as well as glutathione.

• American whey protein is unflavored, however it does contains xanthan gum

• Both American Whey and NutriBio are whey protein isolates with protein levels at

around 90%.

About Soy protein powder The Solae Company, owned by DuPont, is a leader in developing soy products and ingredients

for food. On their website they tout the healthy benefits of their soy protein and discuss the

sustainability of soy and the limited environmental impact of growing it. While it may well be

true that soy production uses less water and provides more usable protein per acre than other

crops or animals, these markers of sustainability come at a big price. Currently 93% of all soy

grown in the U.S. is genetically modified (Fernandez-‐Cornejo 07/5/2012) ii. A number of studies

over the past decade have revealed that genetically engineered foods can pose serious risks to

humans, domesticated animals, wildlife and the environment. Human health effects can include

higher risks of toxicity, allergenicity, antibiotic resistance, immune-‐suppression and cancer. As for

the environment, genetic modification allows these plants to be sprayed more heavily with

herbicides and pesticides, further polluting our air, land, and water supplies. There are additional

health problems associated with the use of soy protein. Soy contains goitrogens that block

thyroid hormones, lowering metabolism, disrupting endocrine function and can lead to

Hashimotos or Graves autoimmune disorders. There are a number of compounds in the soybean

that inhibit digestion. In addition, soy contains high amounts of phyto (plant) estrogens which

promote high estrogen levels (and hormonal imbalance) in both men and women (Daniel, PhD,

CCN 2005) iii.

There are two soy protein products included: NutriBio and Bulkfoods. Both are pure protein

products with no flavoring or additives.


Nutritional Advantages of Advance Protein Powder

Standardized to 25 grams of protein per serving

Advance Protein Powder

Blue Wave

Source Organic Whey

American Whey

BulkFood Whey Isolate

NutriBio Whey Isolate

NutraBio Soy

Protein Isolate

BulkFood Soy

Protein Isolate

Serving Size 29.2 29.8 28.5 27.5 28.3 28.7 27.5

Calories 100.0 135.0 119.0 104.0 128.8 107.0 110.0 128.8

Protein 25.0 25.0 25.0 25.0 25.0 25.0 25.0 25.0

Carbohydrates 0.3 3.0 2.4 1.4 1.3 1.0 0.0 0.0

Fat in gm 0.0 2.1 1.8 0.0 0.6 1.0 1.0 1.3

Saturated Fats 0.0 2.1 0.6 0.0 NA 1.0 NA NA

Cholesterol 0.0 31.3 47.6 4.5 NA 2.0 NA NA

* All Advance International, Inc products are third-‐party tested by Eurofins Scientific S.E.

Additional information about the various products

APP Blue Wave

Source Organic Whey

American Whey

Whey-‐Isolate

NutriBio Whey

NutriBio Soy

Bulk Soy Isolate

Digestibility 98.1% within 1 hour *

NA NA NA NA NA NA NA

Shelf Life 5 years * 2 years 2 years 2 years 2 years 2 years 2 years 2 years

Taste NONE strong fish

mild milk mild milk mild mild

milk mild soy mild soy

Odor NONE strong fish

mild milk mild milk mild mild

milk mild mild

GMO NO no no yes (feed)

yes (feed)

yes (feed)

yes (seed)

yes (seed)



APP has a high composition of naturally occurring organic minerals

The chart below compares the mineral content of the eight protein products.

Comparing mineral content per 25gm of protein as a percentage of the RDA

APP Blue Wave

Source Organic Whey

American Whey

Whey-‐Isolate

NutriBio Whey

NutriBio Soy

Bulk Soy

Isolate

Calcium 64.3% 12.5% 11.9% 12.0% 12.5% 14.0% 5.7% 4.7% Iron 21.3% 0% 0% 0% 0% 0% 22.2% 18.2% Magnesium 11.7% 0% 9.5% 0% 0% 5.0% 0% 4.9% Zinc 11.7% 0% 0% 0% 0% 0% 0% 0% Selenium 0.1% 0% 0% 0% 0% 0% 0% 0% Copper 6.7% 0% 0% 0% 0% 0% 0% 0% Chromium 0.1% 0% 0% 0% 0% 0% 0% 0% Sodium 2.4% 0% 0% 1.8% 0% 0% 16.8% 10.4% Potassium 4.5% 0% 0% 2.4% 0% 0% 0% 7.1% Phosphorus 18.4% 0% 9.5% 5.9% 0% 6.0% 0% 26.6%


Because APP uses all parts of the fish including bones and skin, it has a naturally occurring, balanced organic mineral profile, something not possible in other protein powders. Note the especially good levels of calcium with a detail of the benefits below:

• The CDCP (Center for Disease Control & Prevention) estimates most Americans

consume only 40-‐50% of the RDA of calcium.

• Most supplements and foods are fortified with inferior forms of calcium that

are poorly utilized within the body.

• The calcium in APP is specific to the fish it is produced from and is therefore a

synergistic component with all the nutrients available. And, because protein

enhances calcium absorption it becomes more bio-‐available (Kerstetter et al.

2003).iv

• Calcium is needed for blood pressure, heartbeat, and nerve regulation

(Beauchamp 2006).v

• It is vital for bones, teeth, and epithelial (skin, lung, gut) tissue.


• Calcium is an important component of immune function (Alastra 2011).vi

• It strongly correlates to weight regulation and loss (Université Laval 2009).vii

APP is easily digestible with a high Biological Value (BV) Digestion is vital to all nutrient absorption and assimilation. Many people have compromised

digestive ability due to factors such as pharmaceutical drug use and age-‐related reduction in

digestive enzyme production. Fish in general is one of the easiest proteins to digest, while

many people have problems digesting the proteins in dairy and soy products.

The BV value of APP is similar to that of Whey Protein and far superior to that of Soy.

APP has fewer calories per 25 grams of protein due to the absence of carbohydrates and fats

in the finished product.

Fish Protein Research Research on fish protein (FP) supports the following health benefits over other forms of

protein. It has been most often compared with casein, the protein found in milk.

The following qualities have been reported in various research studies involving fish protein:

• FP has been shown to lower LDL cholesterol levels as well as increase HDL,

improving the ratios while lowering total cholesterol (Bergeron 1989)viii, (Bergeron

1992).ix

• One study showed lower triglyceride levels, reduced weight gain and lower blood

pressure from FP use. The report suggests the amino acid ratios may be a

significant contributor to these findings (Ait-‐Yahia 2003)x (Ait Yahia 2005).xi

• FP can lower C-‐Reactive Protein (a blood marker for inflammation) which has a

strong correlation with CVD, and improve insulin sensitivity in people with a BMI >25

(Ouellet 2008).xii


• Fish protein peptides can decrease hypertensive symptoms (Kawasaki 2002).xiii

• Studies show FP increases immune response (Tang 2008).xiv

• FP has demonstrated the ability to restore intestinal integrity (Cinq-‐Mars 2008).xv

• Fish protein improves insulin sensitivity in insulin-‐resistant men and women

(Ouellet et al. 2007).xvi

All Natural, Environmentally Friendly, Green Manufacturing Process and Sustainable Abundant Sourcing Advance Protein Powder is naturally sourced from multiple, abundant, and sustainable wild

marine-‐based sources worldwide. These include a wide variety of green-‐list fish such as

sardines, macros and other similar species in addition to having the widely available option of

utilizing what is known as "bycatch". Bycatch are very small fish either caught unintentionally

or purchased as imperfect discards from processing facilities; such as in sardine canning. APP

can also be produced from what some consider a waste product of the fishing industry. In

contrast to whey though, the bycatch used here are not exposed to toxic feeds, as are

commercial dairy cows. APP is completely wild and not sourced from any farmed fish or

farmed fish byproducts and contains no GMO. The small and abundant species of fish used as

the raw material make APP an earth and ocean friendly product with a low carbon footprint.

Eurofins Testing Samples of APP have undergone multiple rounds of analysis by Eurofins Scientific, Inc., a

leader in food, pharma, and environmental laboratory testing. Because of the small size of

the fish, the heavy metal/toxin exposure is negligible. All tests have confirmed mercury, lead,

and other metals at <0.05 ppm. This is well below International Pharmaceutical Standard of

0.1 ppm. Further analysis by Eurofins showed a very high rate -‐ or ease -‐ of digestibility

(using a Pepsin Test) making the amino acids and minerals very bio-‐available. Advance

Protein Powder has a long, stable shelf life; a sample produced in 2005 and tested in 2012

showed a profile consistent with a current batch analysis.


Potential for Allergic Response All three protein sources discussed here are among the top eight most allergenic foods

known. These eight foods include: milk/dairy, eggs, soy, peanuts, tree nuts, shellfish, finfish,

and wheat (in no particular order). Food allergies can be a problem for people and in some

cases a very serious one. The CDC's (Centers for Disease Control and Prevention) National

Center for Health Statistics reports that these foods account for 90 percent of all food

allergies. According to the National Institute of Health (NIH), allergy to eggs, milk, peanuts,

tree nuts, soy, and wheat are most likely to occur in children. In Adults, the most common

foods that cause allergic (anaphylaxis) reactions are shellfish, peanut and tree nuts, and then

fish.

The following data is taken in part from a report from FARE, a nonprofit organization for

Food Allergy Research & Education) xvii

Peanuts: 0.6 -‐ 1.3%

Tree nuts: 0.4 -‐ 0.6%

Fish: 0.4%

Crustacean shellfish (crab, shrimp, lobster...): 1.2%

All seafood: 0.6% in children and 2.8% in adults

Milk: 2.5% in young children and 0.2 -‐ 0.4% in general population

Soy: 0.4% in children and 0.1% in adults xviii


Potential retail food uses for APP include:

• Food bars with a varied protein, fat, & carbohydrate ratios and ingredients

o Sports nutrition: bodybuilders, climbers, runners, etc. for both pre and post

workout. Private label options for gyms and athletic clubs

o Snacks: healthy alternatives for kids and adults

o Specialty: blood sugar regulation and weight loss

• Drink powder blends and pre-‐blended drinks

o Formulation options for the categories listed above with higher protein and

additional nutrition potential than possible in bars

o Meal replacement "on the go" drinks for regular use

o Meal replacement drinks or blends for weight loss and detoxification

o Geriatric nutrition drinks

o Balanced nutrition during pregnancy

Potential therapeutic uses for APP include:

• Rapidly reversing the effects of severe malnutrition in children and adults in

global hotspots

• Clinical use in a physical therapy setting to assist in bone and tissue growth

after injury and surgery

• As a nutritional supplement and calcium source for people with osteopenia

and osteoporosis

• To reduce incidence of sarcopenia in the aged and infirm

• As a superior protein source in a hospital or rehabilitation setting to replace

the soy protein in widely available pre-‐made "nutrition" drinks


In Summary Advance Protein Powder is an exciting new product and proprietary process technology with

high potential as an all-‐natural protein/mineral supplement, in multiple retail food products,

and for use in therapeutic nutritional therapies. APP is comparable in BV value to the best

quality whey protein products on the market and superior in balanced nutrition due to the

unique amino acid profile and abundance of naturally occurring organically bound minerals

found in fish.

Julie Spero, MBA, NC

President

i “Nutrition and Energy Bars “Wise Traditions in Food, Farming and the Healing Arts”. Weston A. Price Foundation, Winter 2002. http://www.westonaprice.org/index.html

ii Fernandez-‐Corenego, Jorge. USDA, National Agricultural Statistics Servce, “Recent Trends in GE Adoption.” Last modified 07/05/2012. http://www.ers.usda.gov/data-‐products/adoption-‐of-‐genetically-‐engineered-‐crops-‐in-‐the-‐us/recent-‐trends-‐in-‐ge-‐adoption.aspx.

iii Daniel, PhD, CCN, Kaayla T. The Whole Soy Story. Washington, DC: New Trends Publishing, Inc., 2005.

iv Jane E. Kerstetter, Kimberly O. O'Brien, and Karl L. Insogna, "Low Protein Intake: The impact of Calcium and Bone Homeostasis in Humans," The Journal of Nutrition, 133, no. 3 (2003): 8555-‐8615.

v Beauchamp, Kimberley, ND. Bastyr Center for Natural Health, “Calcium Reduces High Blood Pressure.” Last modified 2006. http://www.bastyrcenter.org/content/view/1029.

vi Alastra, Holly. “The Role of Calcium in the Bones and Immune System.” Last modified 2011. http://www.livestrong.com/article/526824-‐the-‐role-‐of-‐calcium-‐in-‐the-‐bones-‐and-‐immune-‐system/.

vii Université Laval (2009, March 19). “Diet Rich in Calcium Aids Weight Loss in People with Calcium Deficient Diets”. Science Daily.

viii N. Bergeron, H. Jacques, “Influence of fish protein as compared to casein and soy protein on


serum and liver lipids, and serum lipoprotein cholesterol levels in the rabbits”. Atherosclerosis, August, no. 78 (1989): 113-‐21.

ix N. Bergeron, Y. Deshailes, H. Jacques, “Factorial experiment to determine influence of fish protein and fish oil on serum and liver lipids in rabbits”. Nutrition, Sept-‐Oct, no. 8 (1992): 354-‐8

x D. Ait-‐Yahia, et al, “Dietary fish protein lowers blood pressure and alters tissue polyunsaturated fatty acid composition in spontaneously hypertensive rats.,” Nutrition, April, no. 19 (2003): 342-‐6

xi D. Ait-‐Yahia, et al, “Fish protein improves blood pressure but alters HDL2 and HDL3 composition and tissue lipoprotein lipase activities in spontaneously hypertensive rats.,” European Journal of Nutrition, 44, no. (2005): 10-‐15

xii Veronique Oullet, et al, “Dietary Cod Protein Reduces Plasma C-‐Reactive Protein in Insulin-‐Resistant Men and Women,” The Journal of Nutrition, 138, no. December (2008): 2386-‐2391.

xiii T Kawasaki, “Antihypertensive effect and safety evaluation of vegetable drink with peptides derived from sardine protein hydrolysates on mild hypertensive, high-‐normal and normal blood pressure subjects,” Center for Health and Sports Science, 93, no. Oct (2002): 208-‐18

xiv Hong-‐gang Tang, et al, “Effects of fish protein hydrolysate on growth performance and humoral immune response in large yellow croaker (Pseudosciaena crocea R.),” Journal of Zhejiang University SCIENCE B, 9, no.9 (2008): 684-‐690.

xv Crystal D. Cinq-‐Mars, “Investigations into inhibitor Type and Mode, Simulated Gastrointestinal Digestion and Cell Transport of the Angiotensin I-‐Converting Enzyme-‐Inhibitory Peptides in Pacific Hake (Merluccius productus) Fillet Hydrolysate,” Journal of Agricultural and Food Chemistry, 52, no. 2 (2008): 410-‐410

xvi Ouellet, Veronique, Julie Marois, and S. John Weisnagel. "Dietary Cod Protein Improves Insulin Sensitivity in Insulin-‐Resistant Men and Women." Diabetes Care. no. 11 (2007): 2816-‐2821.

xvii FARE: Food Allergy Research & Education, “Food Allergy Facts and Statistics for the U.S.” Last modified 10/3/2011. http://www.foodallergy.org/document.doc?id=194.

xviii Savage JH, Kaeding AJ, Matsui EC, Wood RA. The natural history of soy allergy. J Allergy Clin Immunol, 2010;125:683-‐86


AMINO ACID COMPOSITION WITH COMPARISON GRAPHS

Advance Protein Powder

*

Blue Wave

Source Organic Whey

American Whey

Whey-‐Isolate

NutriBio Whey

NutriBio Soy

Bulk Soy

Isolate

Essential

Histidine 0.36 0.21 0.23 0.48 0.47 0.42 0.66 NA Isoleucine* 0.59 0.54 1.17 1.87 1.75 1.58 1.24 1.50 Leucine* 1.02 1.01 2.42 2.63 3.03 2.74 2.07 2.50 Lysine* 1.14 0.93 1.95 2.20 3.03 2.74 1.58 NA Methionine 0.73 0.45 0.50 0.65 0.63 0.57 0.35 0.41 Phenylalanine 0.54 0.57 0.47 0.88 0.91 0.82 1.32 NA Threonine 0.63 0.72 1.71 1.80 1.91 1.72 0.95 1.16 Tryptophan 0.31 0.60 0.23 0.51 0.50 0.45 0.35 0.38 Valine 0.67 0.70 1.03 1.48 1.44 1.30 1.26 1.50

Non

Essential

Alanine 0.85 1.12 0.94 1.27 1.44 1.30 1.09 1.34 Arginine 0.87 1.15 0.31 0.08 0.66 0.59 1.93 2.34 Aspartic Acid 1.33 1.43 2.50 3.45 3.03 2.74 2.93 3.59 Cysteine 0.24 0.70 0.08 0.75 0.81 0.73 0.32 0.38 Glutamic Acid 1.93 2.11 5.32 4.70 NA 4.83 4.86 NA Glycine 0.74 2.03 0.39 0.48 NA 0.45 1.06 NA Proline 0.55 1.02 1.48 1.54 1.72 1.55 1.29 1.63 Serine 0.48 0.98 1.09 1.28 1.16 1.05 1.32 1.69 Tyrosine 0.45 0.46 0.47 0.69 0.84 0.76 0.98 NA

* All Advance International, Inc products are third-‐party tested for quality by Eurofins Scientific S.E.


0.00

1.00

2.00

3.00

4.00

5.00

6.00

Advance Protein Powder *

BlueWave

Source Organic Whey

American Whey

Whey-‐Isolate

NutriBio Whey

NutriBio Soy

Bulk Soy Isolate

AMINO ACID COMPARISON PER 25 GRAMS OF PROTEIN


MINERAL COMPOSITION

RDA MINERALS APP * BlueWave

Source Organic Whey

American Whey

Whey-‐Isolate

NutriBio Whey

NutriBio Soy

Bulk Soy Isolate

1000 mg Calcium 64.3% 12.5% 11.9% 12.0% 12.5% 14.0% 5.7% 4.7%

18 mg Iron 21.3% 0% 0% 0% 0% 0% 22.2% 18.2%

400 mg Magnesium 11.7% 0% 9.5% 0% 0% 5.0% 0% 4.9%

15 mg Zinc 11.7% 0% 0% 0% 0% 0% 0% 0%

70 mcg Selenium 0.1% 0% 0% 0% 0% 0% 0% 0%

2 mg Copper 6.7% 0% 0% 0% 0% 0% 0% 0%

120 mcg Chromium 0.1% 0% 0% 0% 0% 0% 0% 0%

2400 mg Sodium 2.4% 0% 0% 1.8% 0% 0% 16.8% 10.4

% 350

0 mg Potassium 4.5% 0% 0% 2.4% 0% 0% 0% 7.1%

1000 mg

Phosphorus 18.4% 0% 9.5% 5.9% 0% 6.0% 0% 26.6

% * All Advance International, Inc products are third-‐party tested for quality by Eurofins Scientific S.E.


0.0%

10.0%

20.0%

30.0%

40.0%

50.0%

60.0%

70.0%

APP *

BlueWave

Source Organic Whey

American Whey

Whey-‐Isolate

NutriBio Whey

NutriBio Soy

Bulk Soy Isolate

Mineral Content as a percentage of RDA in 25grams of protein

advance protein powder nutritional report

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