adelaide desalination toxicity assessment of an outfall

ecotox SERVICES AUSTRALASIA

Toxicity Assessment of an Outfall Sample from the Adelaide Desalination Plant

Adelaide Aqua

Test Report

March 2013


ECOTOX Services Australas i a Pty Ltd ABN >45 094 714 904

un i t 27 / 2 chap l in drive l a n e cove nsw 2066 T >6 1 2 9420 9481 F >61 2 9420 94 8 4 W > w ww . eco t ox.com . au

Toxicity Assessment of an Outfall Sample from the Adelaide Desalination Plant

Adelaide Aqua

Test Report

March 2013

A NATA

V WORLO RECOGNISEO

ACCREDITATION

ECOTOX Services Australasia Pty Ltd ABN>45 094 714 904

unit 27/2 chaplin drive lane cove nsw 2• 066 T•~~1• 2 9420 9481

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Toxicity Test Report: TR0997/1 (Page 1 of 2)

This document is issued in accordance with NATA’s accreditation requirements

Client: Adelaide Aqua ESA Job #: PR0997 16 Chrysler Rd Date Sampled: 25 March 2013 Lonsdale SA 5160 Date Received: 27 March 2013

Attention: Vanessa Ayala Sampled By: Client Client Ref: Not supplied ESA Quote #: PL0997_q01

Lab ID No.: Sample Name: Sample Description: 5931 Outfall Aqueous sample, pH 8.0, salinity 67.5‰, total ammonia <2.0mg/L*.

Sample received at 23ºC in apparent good condition 5932 Intake Aqueous sample, pH 8.2, salinity 37.2‰, total ammonia <2.0mg/L*.

Sample received at 23ºC in apparent good condition *Ammonia analysis is not covered by Ecotox Services Australasia’s scope of accreditation

Test Performed: 72-hr sea urchin larval development test using Heliocidaris tuberculata Test Protocol: ESA SOP 105 (ESA 2010), based on APHA (1998), Simon and

Laginestra (1996) and Doyle et al. (2003) Test Temperature: The test was performed at 20±1°C. Deviations from Protocol: Nil Comments on Solution The sample 5931 ‘Outfall’ was serially diluted with sample 5932 Preparation: ‘Intake’ (diluent control) to achieve the test concentrations. A FSW

control and diluent control were tested concurrently with the sample. Source of Test Organisms: Field collected from South Maroubra, NSW. Test Initiated: 8 April 2013 at 1515h

Sample 5931: Outfall Concentration % Normal

(%) larvae (Mean ± SD)

Vacant Vacant

FSW Control 95.8 ± 2.2 Diluent Control 91.0 ± 1.8 *

6.3 87.5 ± 2.4 12.5 0.0 ± 0.0

25 0.0 ± 0.0 50 0.0 ± 0.0

100 0.0 ± 0.0

72-hr IC10 = 12.9 (12.6-13.1)% 72-hr EC50 = 8.8 (8.7-8.8)% NOEC = 12.5% LOEC = 25% *Significantly lower percentage of normally developed larvae compared with the FSW Control (Homoscedastic t-Test, 1-tailed, P=0.05)

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ACCREDITATION

ECOTOX Services Australasia Pty Ltd ABN>45 094 714 904

unit 27/2 chaplin drive lane cove nsw 2• 066 T•~~1• 2 9420 9481

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QA/QC Parameter Criterion This Test Criterion met? Control mean % normal larvae ≥70.0% 95.8% Yes Reference Toxicant within cusum chart limits 6.0-17.2µg Cu/L 9.7µg Cu/L Yes

Test Report Authorised by: Dr Rick Krassoi, Director on 10 May 2013

Results are based on the samples in the condition as received by ESA.

NATA Accredited Laboratory Number: 14709 This document is issued in accordance with NATA’s accreditation requirements. Accredited for compliance with ISO/IEC 17025. NATA is a signatory to the APLAC mutual recognition arrangement for the mutual recognition of the equivalence of testing, calibration and inspection reports. This document shall not be reproduced except in full.

Citations:

APHA (1998) Method 8810 D. Echinoderm Embryo Development Test. In Standard Methods for the Examination of Water and Wastewater, 20th Ed. American Public Health Association, American Water Works Association and the Water Environment Federation, USA.

Doyle, C.J., Pablo, F., Lim, R.P. and Hyne, R.V. (2003) Assessment of metal toxicity in sediment pore water from Lake Macquarie, Australia. Arch. Environ. Contam. Toxicology, 44(3): 343-350.

ESA (2010) ESA SOP 105 - Sea Urchin Larval Development Test. Issue No. 9. Ecotox Services Australasia, Sydney NSW.

Simon, J. and Laginestra, E.(1997) Bioassay for testing sublethal toxicity in effluents, using gametes of sea urchin Heliocidaris tuberculata. National Pulp Mills Research Program Technical Report No. 20. CSIRO, Canberra, ACT.

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V W~LD AtCOONIMl!D ACCRliDITATION

ECOTOX Services Aust r a l asia Pty Ltd ABN >45 094 714 904

T•':,.~ 1 2 9420 9481 unit 27/2 cha pl in

G;· drive l ane cove ' ,,, ns~. 2066

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F>.6 ~ 2 9420 9484

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This document is issued in accordance with NATA’s accreditation requirements

Client: Adelaide Aqua ESA Job #: PR0997 16 Chrysler Rd Date Sampled: 25 March 2013 Lonsdale SA 5160 Date Received: 27 March 2013

Attention: Vanessa Ayala Sampled By: Client Client Ref: Not supplied ESA Quote #: PL0997_q01

Lab ID No.: Sample Name: Sample Description: 5931 Outfall Aqueous sample, pH 8.0, salinity 67.5‰, total ammonia <2.0mg/L*.

Sample received at 23ºC in apparent good condition 5932 Intake Aqueous sample, pH 8.2, salinity 37.2‰, total ammonia <2.0mg/L*.

Sample received at 23ºC in apparent good condition *Ammonia analysis is not covered by Ecotox Services Australasia’s scope of accreditation

Test Performed: 48-hr larval development test using the mussel Mytilus galloprovincialis Test Protocol: ESA SOP 106 (ESA 2011), based on APHA (1998) and USEPA (1996) Test Temperature: The test was performed at 20±1°C. Deviations from Protocol: The test was extended to 72 hours Comments on Solution The sample 5931 ‘Outfall’ was serially diluted with sample 5932 Preparation: ‘Intake’ (diluent control) to achieve the test concentrations. A FSW

control and diluent control were tested concurrently with the sample. Source of Test Organisms: Gulf of St Vincent, SA Test Initiated: 5 April 2013 at 1300h

Sample 5931: Outfall Concentration % Normal

(%) larvae (Mean ± SD)

Vacant Vacant

FSW Control 85.5 ± 8.5 Diluent Control 88.5 ± 7.1

6.3 83.8 ± 8.3 12.5 0.0 ± 0.0

25 0.0 ± 0.0 50 0.0 ± 0.0

100 0.0 ± 0.0

72-hr IC10 = 6.4%* 72-hr EC50 = 8.7 (8.6-8.8)% NOEC = 6.3% LOEC = 12.5% *95% confidence limits are not reliable

A NATA

V W~LD AtCOONIMl!D ACCRliDITATION

ECOTOX Services Aust r a l asia Pty Ltd ABN >45 094 714 904

T•':,.~ 1 2 9420 9481 unit 27/2 cha pl in

G;· drive l ane cove ' ,,, ns~. 2066

',-·,JY


F>.6 ~ 2 9420 9484

R W ("':''":t- w . e c o t o x . c o m . a u

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QA/QC Parameter Criterion This Test Criterion met? FSW Control mean % normal ≥70% 85.5% Yes Reference Toxicant within cusum chart limits 7.7-15.5µg Cu/L 10.2µg Cu/L Yes

Test Report Authorised by: Dr Rick Krassoi, Director on 10 May 2013

Results are based on the samples in the condition as received by ESA.

NATA Accredited Laboratory Number: 14709 This document is issued in accordance with NATA’s accreditation requirements. Accredited for compliance with ISO/IEC 17025. NATA is a signatory to the APLAC mutual recognition arrangement for the mutual recognition of the equivalence of testing, calibration and inspection reports. This document shall not be reproduced except in full.

Citations:

APHA (1998) Standard Methods for the Examination of Water and Wastewater. 20th Ed. American Public Health Association, American Water Works Association and the Water Environment Federation, Washington, DC, USA.

ESA (2011) Bivalve Larval Development Test. Issue No. 10. Ecotox Services Australasia, Sydney, NSW

USEPA (1996) Bivalve acute toxicity test (embryo larval) OPPTS 850.1055. Ecological Effects Test Guidelines. United States Environmental Protection Agency. Prevention, Pesticides and Toxic Substances. EPA/712/C-96/137.

ecotox SERVICES AUSTRALAS I A

Chain-of-Custody Documentation

TRUE


□ 0

Datasheet ID: 601.2

Last Revised: 30 April 2009

Sample Receipt Notification Attention : Vanessa Ayala

Client : Adelaide Aqua 16 Chrysler Road Lonsdale SA 5160

Email TelephoneFacsimile

: [email protected] : 400827816 :

Date : 27/03/2012

Re : Receipt of samples Pages : 2

ESA Project : PR0686 For Review Additional Documentation Required - Please Respond

Sample Delivery Details

Completed Chain of Custody accompanied samples: Samples received in apparent good condition and correctly bottled: Security seals on sample bottles and esky intact:

YES YES YES

Date samples received Time samples received No. of samples received Sample matrix Sample temperature

: 27/03/2013 : 10:00 : 2 : aqueous : 21-25°C

Comments : Includes 1 x 10L Intake (ESA ID# 5932) and 1 x 2.5L OUTFALL (ESA ID# 5931)

Contact Details

Customer Services Officer : Tina Micevska Telephone : 61 2 9420 9481 Facsimile : 61 2 9420 9484 Email : [email protected]

Please contact customer services officer for all queries or issues regarding samples

Note that the chain-of-custody provides definitive information on the tests to be performed

Ecotox Services Australia ABN 45 094 714 904 Phone : 61 2 9420 9481 Unit 27, 2 Chaplin Drive Fax : 61 2 9420 9484 Lane Cove NSW 2066 Australia Email : [email protected]

Chain-of-Custody/ Service Request Form ecotoxDalasheel ID: 601 .1

SERI/ICES AUSTRALASIALast Revised: 22 January 2013

Customer: ADP Ad~~ tfi2, f{y, 0d ~ Ship To: ~tot .s~~ Contact Name: VOh'le,s..A Attention: .. /'lvt°' 1'11 i'c;ze.tl.S.IZ<Zi ' Phone: (please provide an email address for sample receipt notification) 040082.78/6 Email:

Sampled by: cJ~~- ~ C-bO-O..\A..• f ...rA_¼@_ o..cle.hi~~~V~ ~e.J- .OJ..<....

Sample Sample Name Sample Number and Tests Requested Comments/ Instructions Sample Time Method Volume of - [S..=•"" '°' '"~'""')I Note that testing will be delayed if an

Date Containers I

incomplete chain of custody is received

(exactly as written on the sample (eg. Grab, (eg 2 X 1 L) • Additional treatment of samples (i.e. spiking) (day/month vessel) composite etc.) • Sub-contracted services (i.e. chemical

/year) analyses). Dilutions required (if different than 100% down to 6.25%)

• Sample holding time restriction (if applicable)

I . Sample used for lrt1gation (if applicable)

Note: An MSDS must be attached if Available

ESA Project Number: PRQ to&~

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:2.(/_o~ lJ~ _ rt:"t-5 r-.t?TPALL 2-5 L'i-- \ - --- -- - - --- ------ - --

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Of: Time:

\ 2~ _g C) Of:

(::<y\ Time:

1.J ):) Of: Time: Of: Time:

Note that the chain-of-custody documentation will provide definitive information on the tests to be performed.

Ecotox Services Australasia. Unit 27, 2 Chaplin Drive, Lane Cove NSW 2066 AUSTRALIA Phone: 61 2 9420-9481 Fax 61 2 9420-9484 [email protected] Page __ of __

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Statistical Printouts for the Sea Urchin Larval Development Test

Sea Urchin Larval Development Test-Proportion Normal Start Date: 8/04/2013 15:15 Test ID: PR0997/02 Sample ID: OUTFALL End Date: 11/04/2013 15:00 Lab ID: 5931 Sample Type: AQ-Aqueous Sample Date: Protocol: ESA 105 Test Species: HT-Heliocidaris tuberculata Comments:

Conc-% 1 2 3 4 FSW Control 0.9800 0.9500 0.9300 0.9700

Diluent Control 0.9000 0.8900 0.9300 0.9200 6.3 0.9000 0.8600 0.8500 0.8900

12.5 0.0000 0.0000 0.0000 0.0000 25 0.0000 0.0000 0.0000 0.0000 50 0.0000 0.0000 0.0000 0.0000

100 0.0000 0.0000 0.0000 0.0000

Transform: Arcsin Square Root 1-Tailed Number Total Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Resp Number

FSW Control 0.9575 1.0522 1.3685 1.3030 1.4289 4.062 4 Diluent Control 0.9100 1.0000 1.2672 1.2327 1.3030 2.531 4 * 36 400

*6.3 0.8750 0.9615 1.2105 1.1731 1.2490 2.985 4 2.346 1.943 0.0469 50 400 12.5 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400

25 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400 50 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400

100 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400

Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.05) 0.87083 0.818 0.040145 -2.17718 F-Test indicates equal variances (p = 0.85) 1.269665 47.46723 The control means are significantly different (p = 0.02) 3.156177 2.446912 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.028551 0.031353 0.006423 0.001167 0.057379 1, 6 Treatments vs Diluent Control

Trimmed Spearman-Karber Trim Level EC50 95% CL

0.0% 5.0% 8.7533 8.6912 8.8159

10.0% 8.7533 8.6912 8.8159 20.0% 8.7533 8.6912 8.8159

Auto-3.8% 8.7533 8.6912 8.8159

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Page 1 ToxCalc v5.0.23 Reviewed by:_____


Dose-Response Plot

1-tail, 0.05 level of significance

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Auxiliary Data Summary Conc-% Parameter Mean Min Max SD CV% N

FSW Control % Normal 95.75 93.00 98.00 2.22 1.56 4 Diluent Control 91.00 89.00 93.00 1.83 1.48 4

6.3 87.50 85.00 90.00 2.38 1.76 4 12.5 0.00 0.00 0.00 0.00 4

25 0.00 0.00 0.00 0.00 4 50 0.00 0.00 0.00 0.00 4

100 0.00 0.00 0.00 0.00 4 FSW Control pH 8.10 8.10 8.10 0.00 0.00 1

Diluent Control 8.10 8.10 8.10 0.00 0.00 1 6.3 8.10 8.10 8.10 0.00 0.00 1

12.5 8.10 8.10 8.10 0.00 0.00 1 25 8.10 8.10 8.10 0.00 0.00 1 50 8.10 8.10 8.10 0.00 0.00 1

100 8.00 8.00 8.00 0.00 0.00 1 FSW Control Salinity ppt 33.10 33.10 33.10 0.00 0.00 1

Diluent Control 37.30 37.30 37.30 0.00 0.00 1 6.3 39.10 39.10 39.10 0.00 0.00 1

12.5 41.30 41.30 41.30 0.00 0.00 1 25 45.10 45.10 45.10 0.00 0.00 1 50 52.70 52.70 52.70 0.00 0.00 1

100 67.80 67.80 67.80 0.00 0.00 1 FSW Control DO % 106.40 106.40 106.40 0.00 0.00 1

Diluent Control 93.60 93.60 93.60 0.00 0.00 1 6.3 104.20 104.20 104.20 0.00 0.00 1

12.5 107.70 107.70 107.70 0.00 0.00 1 25 107.70 107.70 107.70 0.00 0.00 1 50 108.70 108.70 108.70 0.00 0.00 1

100 106.90 106.90 106.90 0.00 0.00 1



Conc-% 1 2 3 4 FSW Control 0.9800 0.9500 0.9300 0.9700

Diluent Control 0.9000 0.8900 0.9300 0.9200 6.3 0.9000 0.8600 0.8500 0.8900

12.5 0.0000 0.0000 0.0000 0.0000 25 0.0000 0.0000 0.0000 0.0000 50 0.0000 0.0000 0.0000 0.0000

100 0.0000 0.0000 0.0000 0.0000

Transform: Arcsin Square Root 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean

FSW Control 0.9575 1.0522 1.3685 1.3030 1.4289 4.062 4 Diluent Control 0.9100 1.0000 1.2672 1.2327 1.3030 2.531 4 * 0.9100 1.0000

*6.3 0.8750 0.9615 1.2105 1.1731 1.2490 2.985 4 2.346 1.943 0.0469 0.8750 0.9615 12.5 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000

25 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000 50 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000

100 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000

Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.05) 0.87083 0.818 0.040145 -2.17718 F-Test indicates equal variances (p = 0.85) 1.269665 47.46723 The control means are significantly different (p = 0.02) 3.156177 2.446912 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates significant differences 0.028551 0.031353 0.006423 0.001167 0.057379 1, 6 Treatments vs Diluent Control

Log-Logit Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew IC05 6.3374 0.6757 2.4060 6.4645 -2.6579 IC10 6.4777 0.0416 6.3445 6.5975 -0.2364 IC15 6.5945 0.0395 6.4647 6.7087 -0.2168 1.0 IC20 6.6972 0.0382 6.5700 6.8046 -0.2003 0.9IC25 6.7908 0.0372 6.6662 6.8966 -0.1867 IC40 7.0441 0.0352 6.9274 7.1451 -0.1588 0.8 IC50 7.2076 0.0343 7.0948 7.3072 -0.1472 0.7

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6.3 87.50 85.00 90.00 2.38 1.76 4 12.5 0.00 0.00 0.00 0.00 4

25 0.00 0.00 0.00 0.00 4 50 0.00 0.00 0.00 0.00 4

100 0.00 0.00 0.00 0.00 4 FSW Control pH 8.10 8.10 8.10 0.00 0.00 1

Diluent Control 8.10 8.10 8.10 0.00 0.00 1 6.3 8.10 8.10 8.10 0.00 0.00 1

12.5 8.10 8.10 8.10 0.00 0.00 1 25 8.10 8.10 8.10 0.00 0.00 1 50 8.10 8.10 8.10 0.00 0.00 1


Diluent Control 37.30 37.30 37.30 0.00 0.00 1 6.3 39.10 39.10 39.10 0.00 0.00 1

12.5 41.30 41.30 41.30 0.00 0.00 1 25 45.10 45.10 45.10 0.00 0.00 1 50 52.70 52.70 52.70 0.00 0.00 1

100 67.80 67.80 67.80 0.00 0.00 1 FSW Control DO % 106.40 106.40 106.40 0.00 0.00 1

Diluent Control 93.60 93.60 93.60 0.00 0.00 1 6.3 104.20 104.20 104.20 0.00 0.00 1

12.5 107.70 107.70 107.70 0.00 0.00 1 25 107.70 107.70 107.70 0.00 0.00 1 50 108.70 108.70 108.70 0.00 0.00 1

100 106.90 106.90 106.90 0.00 0.00 1



Statistical Printouts for the Mussel Toxicity Tests

Bivalve Larval Development Test-Proportion Alive/Normal Start Date: 5/04/2013 13:00 Test ID: PR0997/01 Sample ID: Outfall End Date: 8/04/2013 13:00 Lab ID: 5931 Sample Type: AQ-Aqueous Sample Date: Protocol: ESA 106 Test Species: MG-Mytilus galloprovincialis Comments:

Conc-% 1 2 3 4 FSW Control 0.7300 0.9200 0.8800 0.8900

Diluent Control 0.8400 0.9500 0.8100 0.9400 6.3 0.8900 0.7200 0.8400 0.9000

12.5 0.0000 0.0000 0.0000 0.0000 25 0.0000 0.0000 0.0000 0.0000 50 0.0000 0.0000 0.0000 0.0000

100 0.0000 0.0000 0.0000 0.0000

Transform: Arcsin Square Root 1-Tailed Number Total Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Resp Number

FSW Control 0.8550 0.9661 1.1896 1.0244 1.2840 9.563 4 Diluent Control 0.8850 1.0000 1.2369 1.1198 1.3453 9.213 4 * 46 400

6.3 0.8375 0.9463 1.1636 1.0132 1.2490 9.246 4 0.936 1.943 0.1523 65 400 12.5 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400

25 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400 50 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400

100 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 400 400

Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.05) 0.872471 0.818 -0.45893 -1.78744 F-Test indicates equal variances (p = 0.93) 1.12217 47.46723 The control means are not significantly different (p = 0.58) 0.588233 2.446912 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.110901 0.124244 0.010761 0.01228 0.38535 1, 6 Treatments vs Diluent Control

Trimmed Spearman-Karber Trim Level EC50 95% CL

0.0% 5.0%

10.0% 20.0%

Auto-5.4%

8.7034 8.7034 8.7034

8.6286 8.6286 8.6286

8.7787 8.7787 8.7787

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6.3 83.75 72.00 90.00 8.26 3.43 4 12.5 0.00 0.00 0.00 0.00 4

25 0.00 0.00 0.00 0.00 4 50 0.00 0.00 0.00 0.00 4

100 0.00 0.00 0.00 0.00 4 FSW Control pH 8.20 8.20 8.20 0.00 0.00 1

Diluent Control 8.20 8.20 8.20 0.00 0.00 1 6.3 8.20 8.20 8.20 0.00 0.00 1

12.5 8.20 8.20 8.20 0.00 0.00 1 25 8.10 8.10 8.10 0.00 0.00 1 50 8.10 8.10 8.10 0.00 0.00 1


Diluent Control 37.20 37.20 37.20 0.00 0.00 1 6.3 39.40 39.40 39.40 0.00 0.00 1

12.5 41.60 41.60 41.60 0.00 0.00 1 25 45.30 45.30 45.30 0.00 0.00 1 50 52.70 52.70 52.70 0.00 0.00 1

100 67.50 67.50 67.50 0.00 0.00 1 FSW Control DO % 102.50 102.50 102.50 0.00 0.00 1

Diluent Control 96.60 96.60 96.60 0.00 0.00 1 6.3 98.30 98.30 98.30 0.00 0.00 1

12.5 105.20 105.20 105.20 0.00 0.00 1 25 105.80 105.80 105.80 0.00 0.00 1 50 105.00 105.00 105.00 0.00 0.00 1

100 101.90 101.90 101.90 0.00 0.00 1



Conc-% 1 2 3 4 FSW Control 0.7300 0.9200 0.8800 0.8900

Diluent Control 0.8400 0.9500 0.8100 0.9400 6.3 0.8900 0.7200 0.8400 0.9000

12.5 0.0000 0.0000 0.0000 0.0000 25 0.0000 0.0000 0.0000 0.0000 50 0.0000 0.0000 0.0000 0.0000

100 0.0000 0.0000 0.0000 0.0000

Transform: Arcsin Square Root 1-Tailed Isotonic Conc-% Mean N-Mean Mean Min Max CV% N t-Stat Critical MSD Mean N-Mean

FSW Control 0.8550 0.9661 1.1896 1.0244 1.2840 9.563 4 Diluent Control 0.8850 1.0000 1.2369 1.1198 1.3453 9.213 4 * 0.8850 1.0000

6.3 0.8375 0.9463 1.1636 1.0132 1.2490 9.246 4 0.936 1.943 0.1523 0.8375 0.9463 12.5 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000

25 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000 50 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000

100 0.0000 0.0000 0.0500 0.0500 0.0500 0.000 4 0.0000 0.0000

Auxiliary Tests Statistic Critical Skew Kurt Shapiro-Wilk's Test indicates normal distribution (p > 0.05) 0.872471 0.818 -0.45893 -1.78744 F-Test indicates equal variances (p = 0.93) 1.12217 47.46723 The control means are not significantly different (p = 0.58) 0.588233 2.446912 Hypothesis Test (1-tail, 0.05) MSDu MSDp MSB MSE F-Prob df Homoscedastic t Test indicates no significant differences 0.110901 0.124244 0.010761 0.01228 0.38535 1, 6 Treatments vs Diluent Control

Log-Logit Interpolation (200 Resamples) Point % SD 95% CL(Exp) Skew IC05* 5.4786 1.9599 0.0000 7.0284 -0.3530 IC10 6.4147 0.8524 1.6129 6.6591 -2.4132 IC15 6.5222 0.2767 6.1535 6.7613 -5.5231 1.0 IC20 IC25

6.6187 6.7080

0.1005 0.0973

6.2836 6.3785

6.8615 6.9479

-0.4233 -0.3674

0.9

IC40 6.9546 0.0915 6.6628 7.1901 -0.2501 0.8 IC50 7.1164 0.0889 6.8429 7.3489 -0.2003 0.7 * indicates IC estimate less than the lowest concentration

0.6

0.5

0.4

0.3

0.2

0.1

0.0

Res

pons

e

1 10 100

Dose %



Dose-Response Plot


0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

Prop

ortio

n A

live/

Nor

mal

FSW

Con

trol

Dilu

ent C

ontro

l

6.3

12.5

25 50 100





6.3 83.75 72.00 90.00 8.26 3.43 4 12.5 0.00 0.00 0.00 0.00 4

25 0.00 0.00 0.00 0.00 4 50 0.00 0.00 0.00 0.00 4

100 0.00 0.00 0.00 0.00 4 FSW Control pH 8.20 8.20 8.20 0.00 0.00 1

Diluent Control 8.20 8.20 8.20 0.00 0.00 1 6.3 8.20 8.20 8.20 0.00 0.00 1

12.5 8.20 8.20 8.20 0.00 0.00 1 25 8.10 8.10 8.10 0.00 0.00 1 50 8.10 8.10 8.10 0.00 0.00 1


Diluent Control 37.20 37.20 37.20 0.00 0.00 1 6.3 39.40 39.40 39.40 0.00 0.00 1

12.5 41.60 41.60 41.60 0.00 0.00 1 25 45.30 45.30 45.30 0.00 0.00 1 50 52.70 52.70 52.70 0.00 0.00 1

100 67.50 67.50 67.50 0.00 0.00 1 FSW Control DO % 102.50 102.50 102.50 0.00 0.00 1

Diluent Control 96.60 96.60 96.60 0.00 0.00 1 6.3 98.30 98.30 98.30 0.00 0.00 1

12.5 105.20 105.20 105.20 0.00 0.00 1 25 105.80 105.80 105.80 0.00 0.00 1 50 105.00 105.00 105.00 0.00 0.00 1

100 101.90 101.90 101.90 0.00 0.00 1


adelaide desalination toxicity assessment of an outfall

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