addressing the needs for refinement in laboratory fish nc3rs/cefas, weymouth… · ·...
TRANSCRIPT
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Adrian [email protected]
norecopa.no
3Rs resources and guidelines for zebrafish
AddressingtheneedsforrefinementinlaboratoryfishNC3Rs/Cefas,Weymouth,26-28April2016
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National Consensus Platform for theReplacement, Reduction and Refinement of
Animal Experiments
a competence centre for the 3RS
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International consensus meetings
Harmonisation of the Care and Use of:Fish (2005)Wildlife (2008)Fish (2009)Agricultural animals (2012)
http://norecopa.no/consensus-meetings
All presentations and consensus statements are on the internet: a lasting resource
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www.norecopa.no/norecopa/vedlegg/Fish-guidelines.pdf
www.norecopa.no/fish2009
www.norecopa.no/fish2009
Anoverviewofexistingguidelinesforhandling,bleeding,administrationandidentificationtechniques
PennyHawkins,ResearchAnimalsDepartment,RSPCA
Globalupdateonguidelinesforfishresearch
GillyGriffin,CanadianCouncilonAnimalCare(CCAC)
Guidelinesforanaesthesiaandanalgesiaoffish
Gidona Goodman,UniversityofEdinburgh
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So what do we have for zebrafish…?
What qualifies as a guideline? Who decides? Every IACUC protocol?
Norecopa had that question in mind when weproduced our database 3R Guide (3RGuide.info).
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Reporting
Literaturesearch
Planning
Research
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What does Europe have to offer?
European Convention ETS123 for the Protection of Vertebrate AnimalsUsed for Scientific or Other Purposes
‘TheConventionisdesignedprimarily toreduceboththenumberofexperimentsandthenumberofanimalsusedforsuchpurposes… Animalstobeexperimentedonshouldbeselectedonthebasisofclearlyestablishedquantitativecriteriaandmustbewellcaredforandsparedavoidablesufferingwheneverpossible.’
Appendix A: Guidelines for the accommodation and care of animals(revised, with species-specific guidelines from 2007).
‘Species-specificguidanceon rainbowtrout(Oncorhynchusmykiss),Atlanticsalmon(Salmosalar),tilapiine cichlids,zebrafish(Daniorerio),seabass(Dicentrarchuslabrax),Atlantichalibut(Hippoglossus hippoglossus),Atlanticcod(Gadusmorhua),turbot(Scophthalmusmaximus),Africancatfish(Clarias gariepenus)isavailableinthebackgrounddocumentelaboratedbytheGroupofExperts.Furtheradviceontherequirementsoftheseandotherspeciesshouldbesoughtfromexpertspecialistsandcarestafftoensurethatanyparticularspeciesneedsareadequatelyaddressed.’
http://www.felasa.eu/about-us/library/
PartBofAppendixA:Scientificbackground.NoPartBforfish!
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EUROGUIDEOn the accommodation and care On the accommodation and care On the accommodation and care of animals used for experimental of animals used for experimental of animals used for experimental and other scientific purposesand other scientific purposesand other scientific purposes
The Council of Europe is the continent's oldest political organization, founded in 1949. It developsEurope-wide recommendations, agreements and conventions with a view to facilitating memberstates' economic and social progress. Its European Convention for the Protection of VertebrateAnimals used for Experimental and Other Scientific Purposes (ETS 123 of 1986) concerns the careand use of animals in procedures (experiments). The Convention includes Appendix A, whichpresents guidelines for accommodation and care of animals.
From 1997 to 2006 the Parties to the Convention undertook a revision of Appendix A, involvingmany stakeholders representing science, industry, animal welfare, governments and regulators.The revised Appendix A has a General Section – providing guidelines on accommodation and carerelevant to all animals used for experimental and other scientific purposes – and species-specificprovisions for rodents, rabbits, cats, dogs, ferrets, non-human primates, farm animals (cattle,sheep, goats, pigs and mini-pigs, horses), birds, amphibians, reptiles and fish.
The Federation of European Laboratory Animal Science Associations (FELASA) represents nationalassociations across Europe and actively participated in the Appendix A revision process. Readers ofthis Euroguide should remain aware that the official approved Appendix A will always be thedefinitive document that should be consulted for points of certitude for accommodation and careof animals; however, FELASA has produced the Euroguide as a concise and user-friendly referencefor anyone working with laboratory animals and for those who have activities in services relatedto their housing and care.
Financial support for production and distribution of FELASA’s Euroguide has been provided by theEuropean Partnership for Alternative Approaches to Animal Testing (EPAA), a joint initiative by theEuropean Commission and a number of companies and trade federations active in various industrialsectors. The Partnership’s work focuses on mapping existing research, developing new alternativeapproaches and strategies, and promoting communication, education, validation and acceptance ofalternative approaches in replacement, reduction and refinement of the use of laboratory animals.
The Euroguide is published for FELASA with support from Laboratory Animals Ltd, proprietors of itsofficial journal Laboratory Animals.
EUROGUIDEOn the accommodation On the accommodation On the accommodation and care of animals used and care of animals used and care of animals used for experimental and for experimental and for experimental and other scientific purposesother scientific purposesother scientific purposes
BASED ON THE REVISED APPENDIX A OF THE EUROPEAN CONVENTION ETS 123
Published in conjunction with
BASED ON THE REVISED APPENDIX A OF THE EUROPEAN CONVENTION ETS 123
www.lal.org.ukwww.epaa.eu.comwww.felasa.eu
http://www.felasa.eu/media/uploads/Euroguide_official_publication.pdf
7pagesmentionZebrafinches,nothingonZebrafish…
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EU Directive 2010/63 on theProtection of Animals Used for Scientific Purposes
ETS123again!
Furtheradviceontherequirementsoftheseandotherspeciesshouldbesoughtfromexpertspecialistsandcarestafftoensurethatanyparticularspeciesneedsareadequatelyaddressed.
“Fish”(all30,000spp.)!
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ec.europa.eu/animals-in-science
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ExpertWorkingGroupreportonseverityclassification
http://ec.europa.eu/environment/chemicals/lab_animals/pdf/report_ewg.pdf
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Guidance on the severity classification ofprocedures involving fish
Report fromaWorking Groupconvened byNorecopa
DesignedtobeasupplementtotheEUWorkingGroupreportonthesamesubject,which ismostrelevantfor
traditionallabanimals
PHawkins,NDennison,GGoodman,SHetherington,SLlywelyn-Jones,KRyderandAJSmith
LaboratoryAnimals, 45:219-224, 2011www.norecopa.no/categories
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https://www.gov.uk/government/publications/code-of-practice-for-the-housing-and-care-of-animals-bred-supplied-or-used-for-scientific-purposes
ReferstoRSPCAGuidance
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http://science.rspca.org.uk/sciencegroup/researchanimals/implementing3rs/housingandcareaquaticspecies
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http://science.rspca.org.uk/sciencegroup/researchanimals/implementing3rs/housingandcareaquaticspecies
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http://science.rspca.org.uk/sciencegroup/researchanimals/implementing3rs/housingandcareaquaticspecies
1. Introduction2. Backgroundinformationonzebrafish
• Naturalgeographicrangeandhabitat• Speciescharacteristics• Useinresearchandteaching
3. Supplyandtransport• Source• Transportconsiderations• Quarantine
4. Housingandcare• Lighting• Noiseandotherdisturbances• Humidity• Waterprovision• Tankhousing• Identificationandmarkingtechniques• Grouphousing• Catchingandhandling• Foodtypeandfeedingregime• Environmentalenrichment• Assessofhealthanddiseaseprevention
62pages,including8pageswith
literaturereferences
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http://science.rspca.org.uk/sciencegroup/researchanimals/implementing3rs/housingandcareaquaticspecies
5. Scientificprocedures• Eggharvesting• Transgenesis• Mutagenesis• Genotyping• Cryopreservation• Bloodcollection• Injections• Analgesiaandanaesthesia• Humanekilling
6. Trainingofanimalcarestaffandusers7. Concludingcomments8. References
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http://science.rspca.org.uk/sciencegroup/researchanimals/reportsandresources/housingandcare
Africanclawedfrogs(Xenopus laevis)CattleDogsDomesticfowlDucksandgeeseFerretsGuineapigsHamstersMicePigeonsPigsQuailRabbitsRatsSheepZebrafinches
‘Theyareprimarilyintendedformembersofethicalreviewcommittees,thoughshouldalsobeusefulforanimaltechnologists, scientists andlaboratoryanimalveterinarians’.
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http://www.felasa.eu/working-groups/working-groups-present/zebrafish-housing-husbandry-and-health-monitoring-recommendations
FELASAGuidelinesunderdevelopment
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http://www.ccnorway.no/zebrafish
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WorldCongressesonAnimalUseintheLifeSciencesandAlternatives
Important3R-driversanddisseminatorsofinformation:
wc9prague.org (2014)
891abstracts,49countries,1000participants(thenextoneisinSeptember2017inSeattle)
Abstractbook:http://www.altex.ch/ALTEX-Proceedings/Current-Proceedings.97.html
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2005-
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http://online.liebertpub.com/doi/pdfplus/10.1089/zeb.2015.1198
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http://online.liebertpub.com/doi/pdfplus/10.1089/zeb.2015.1210
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http://lan.sagepub.com/content/40/4/323.full.pdf
Needstobefollowedupbyspecies-specificguidelines
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http://www.sciencedirect.com/science/article/pii/S0044848607004012
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EuropeanSocietyforFishModels inBiologyandMedicine (EUFishBioMed)http://www.eufishbiomed.kit.edu
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https://www.eufishbiomed.kit.edu/59.php
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Linkstoallonwww.eufishbiomed.kit.edu/58.php
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www.bazh.org.uk
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www.zebrafish.org
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https://wiki.zfin.org/display/prot/ZFIN+Protocol+Wiki
TheZebrafishBook
http://zfin.org/zf_info/zfbook/zfbk.html
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Zebrafish.no
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Reporting
Literaturesearch
Planning
Research
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Frequent challenges with research on non-traditional species
• Absenceofstandardised animals• Health,housingandwelfaremonitoring• Recognitionofpain,suffering,distress
• “Normal”mortality• Refinement ofprocedures• Techniques foranaesthesia and
humanekilling
http://ichef-1.bbci.co.uk/news/660/media/images/69786000/jpg/_69786238_69786233.j pg
https://speakingofresearch.com/2015/05/11/zebrafi sh_risi ng_star_animal_model
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http://blogs.discovermagazine.com/notrocketscience/2011/01/12/flipper-bands-impair-penguin-survival-and-breeding-success/#.VLU6_8Y7_wo
Photo: T.Poppe, NMBU
”Simple” techniques?
Photo: NMBU
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Laboratory Animals, 2000, 34, 430-433
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film.oslovet.norecopa.no
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Harm-BenefitAssessment
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An useful additional (but largely unknown) tool…Carol M. Newton (1925-2014)
The three S’s
• Good Science• Good Sense*• Good Sensibilities*
*We can do this ourselves without scientific literature!
CarolMNewton,quotedinRowsellHC(1977):TheEthicsofBiomedicalExperimentationinTheFutureofAnimals,Cells,Models,andSystemsinResearch,Development,Education,andTesting pp.267-281,NationalAcademyofSciences,Washington,D.C.,ISBN0-309-02603-2.
NationalLibraryofMedicine
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Criticalanthropomorphism
=empathy + objective,knowledge-basedconsiderationofwhatislikelytobesignificanttotheanimal
NationalLibraryofMedicine
Penny Hawkins, Vosshttp://tjinfo.uib.no/Vedlegg?id=3753833e5de91342a40a2b95a5b4391a
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Do you need more guidelines?
https://commons.wikimedia.org/wiki/File:Truman_pass-the-buck.jpg
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Thankstoourmainsponsors:
• Dag S. Stiansen Foundation• Laboratory Animals Ltd.• Ministries of Agriculture and Fisheries• Nordic Society Against Painful Experiments• Norwegian Research Council• Novo Nordisk• Scottish Accreditation Board
FundingoftheNORINAdatabase:
NordicSocietyAgainstPainfulExperiments,DagS.Stiansen Foundation,TheNorwegianResearchCouncil,theNorwegianSchoolofVeterinaryScience,LaboratoryAnimalsLtd.,RSPCA,UFAW,AstraZeneca,SolvayPharmaceuticals,theSwedishFundforResearchwithoutAnimalExperiments,NorwegianFederationforAnimalProtection,Allkopi,TheHumaneSocietyoftheUnitedStates,St.AndrewAnimalFund,MicrosurgicalDevelopmentsFoundation,AAALACInternational,LASA,NEAVS,AmershamHealth