ACQUITY Advanced Polymer Chromatography (APC) Applications Notebook

[ APPLICATION highlight ]

TABLE OF CONTENTS

LIST OF POLYMERS ANALYZED USING ACQUITY APC

Polymer Part Number Page

Poly(styrene) in THF Calibration Curves WA64641 3

Poly(vinylacetate) in THF WA64642 4

Poly(vinyl sulfone) in THF WA64643 5

Poly(methyl methacrylate co ethyl acrylate) in THF WA64644 6

Poly(vinylidene chloride co acrylonitrile co methyl methacrylate) in THF WA64645 7

Polyurethane in THF WA64646 8

Poly(methylmethacrylate co hydroxyethylmethacrylate) in THF WA64647 9

Epoxy Resin in THF WA64648 10

Poly(vinylpyrrolidone co methyl methacrylate) in THF WA64649 11

Poly(methyl methacrylate) in THF WA64650 12

Poly(phenylglycidyl ether) co formaldehyde in THF WA64652 13

Poly(o-cresyl glycidyl ether) co formaldehyde in THF WA64653 14

Poly(phenyl isocyanate) co formaldehyde in THF WA64654 15

Poly(styrene), Low Molecular Weight in THF WA64655 16

Poly(isobutyl methacrylate) in THF WA64656 17

Poly(bisphenol A co epichlorohydrin) in DMF WA64657 18

Poly(vinylacetate) in DMF WA64658 19

Poly(9,9 di-n-octylfluorenyl 2,7-diyl) in Toluene WA64659 20

Poly(styrene) Standards in THF WA64660 21

Poly(dimethyl silicone) in THF WA64711 22

Poly(dimethyl siloxane) in Toluene WA64712 23

Aqueous Analysis of Pullulans WA64661 24

Poly(styrene) in THF Calibration Curves using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: UV at 254 nm

Temperature: 30 °C

Flow rate: 0.6 mL/min

Samples: Poly(styrene)

2520000

549000

130000

34800

9130

1250

277000

130000

34800

176009130

3470

66000

9130

3470

1250

266

15700

4920

22801250

682370

162100

1,000

10,000

100,000

1,000,000

10,000,000

0.50

Elution Volume (mL)

Mol

ecul

ar W

eigh

t (D

a)

1.00 1.50 2.00

ACQUITY APC™ 450, 2.5 µm

ACQUITY APC 200, 2.5 µm

ACQUITY APC 125, 2.5 µm

ACQUITY APC 45, 1.7 µm

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64641

3

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© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64642

4

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Analysis of Poly(vinylacetate) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (450Å, 125Å and 45Å in series), part numbers 186007010, 186007000 and 186006995, respectively

Flow rate: 1 mL/min

Temperature: 40 °C

Injection volume: 30 µL

Sample load: 0.2% (w/v)

Sample: Poly(vinylacetate)

µRIU

-2.00

0.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

Minutes

2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64643

5

[ APPLICATION highlight ]

Analysis of Poly(vinyl sulfone) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Injection volume: 20 µL

Flow rate: 1 mL/min

Sample load: 0.2% (w/v)

Sample: Poly(sulfone)

-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00

10.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

µRIU

Minutes

6

[ APPLICATION highlight ]

Analysis of Poly(methyl methacrylate co ethyl acrylate) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Flow rate: 1 mL/min

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(methyl methacrylate co ethyl acrylate)

-2.50 -2.00 -1.50 -1.00 -0.50 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

µRIU

Minutes

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64644

7

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Analysis of Poly(vinylidene chloride co acrylonitrile co methyl methacrylate) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Injection volume: 20 µL

Flow rate: 1 mL/min

Sample load: 0.2% (w/v)

Sample: Poly(vinylidene chloride co acrylonitrile co methyl methacrylate)

µRIU

Minutes

-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00

10.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64645

8

[ APPLICATION highlight ]

Analysis of Polyurethane in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (450Å, 125Å and 45Å in series), part numbers 186007010, 186007000 and 186006995, respectively

Flow rate: 1 mL/min

Temperature: 40 °C

Injection volume: 30 µL

Sample load: 0.4% (w/v)

Sample: Polyurethane

-2.00

0.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00

µRIU

Minutes

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64646

9

[ APPLICATION highlight ]

Analysis of Poly(methylmethacrylate co hydroxyethylmethacrylate) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (450Å, 125Å and 45Å in series), part numbers 186007010, 186007000 and 186006995, respectively

Flow rate: 1 mL/min

Temperature: 40 °C

Injection volume: 30 µL

Sample load: 0.4% (w/v)

Sample: Poly(methylmethacrylate co hydroxyethylmethacrylate)

µRIU

Minutes

-2.00

0.00

2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

18.00

20.00

22.00

24.00

2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40 5.60 5.80 6.00

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64647

10

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Analysis of an Epoxy Resin in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (125Å and 45Å in series), part numbers 186007000 and 186006995, respectively

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.5% (w/v)

Sample: Epoxy resin

µRIU

Minutes

-1.00 -0.50 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64648

11

[ APPLICATION highlight ]

Analysis of Poly(vinylpyrrolidone co methyl methacrylate) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (125Å and 45Å in series), part numbers 186007000 and 186006995, respectively

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.5% (w/v)

Sample: Poly(vinylpyrrolidone co methyl methacrylate)

µRIU

Minutes

-2.00

-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00

10.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64649

12

[ APPLICATION highlight ]

Analysis of Poly(methyl methacrylate) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(methyl methacrylate)

µRIU

Minutes

-16.00

-14.00 -12.00 -10.00

-8.00 -6.00 -4.00 -2.00 0.00 2.00 4.00 6.00 8.00

10.00 12.00 14.00 16.00 18.00 20.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64650

13

[ APPLICATION highlight ]

Analysis of poly(phenylglycidyl ether) co formaldehyde in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(phenylglycidyl ether) co formaldehyde

µRIU

Minutes

-40.00

-30.00 -20.00 -10.00

0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64652

14

[ APPLICATION highlight ]

Analysis of Poly(o-cresyl glycidyl ether) co formaldehyde in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(o-cresyl glycidyl ether) co formaldehyde

µRIU

Minutes

-40.00 -30.00 -20.00 -10.00

0.00 10.00 20.00 30.00 40.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64653

15

[ APPLICATION highlight ]

Analysis of Poly(phenyl isocyanate) co formaldehyde in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(phenyl isocyanate) co formaldehyde

µRIU

Minutes

0.00 20.00 40.00 60.00 80.00

100.00 120.00 140.00 160.00 180.00 200.00 220.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64654

16

[ APPLICATION highlight ]

Analysis of Poly(styrene), Low Molecular Weight in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (45Å and 45Å in series), part number 186006995

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(styrene), low molecular weight

µRIU

Minutes

-20.00 -15.00 -10.00

-5.00 0.00 5.00

10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64655

17

[ APPLICATION highlight ]

Analysis of Poly(isobutyl methacrylate) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(isobutyl methacrylate)

µRIU

Minutes

-6.00

-4.00

-2.00

0.00

2.00

4.00

6.00

8.00

0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64656

18

[ APPLICATION highlight ]

Analysis of Poly(bisphenol A co epichlorohydrin) in DMF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: DMF with 10 mM LiCl

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(bisphenol A co epichlorohydrin)

µRIU

Minutes

-1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00

10.00

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64657

19

[ APPLICATION highlight ]

Analysis of Poly(vinylacetate) in DMF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: DMF with 10 mM LiCl

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.2% (w/v)

Sample: Poly(vinylacetate)

µRIU

Minutes

-1.00

-0.50

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64658

20

[ APPLICATION highlight ]

Analysis of Poly(9,9 di-n-octylfluorenyl 2,7-diyl) in Toluene using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: Toluene

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (450Å and 125Å in series), part numbers 186007010 and 186007000, respectively

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 0.5% (w/v)

Sample: Poly(9,9 di-n-octylfluorenyl 2,7-diyl)

µRIU

Minutes

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00 4.20

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64659

21

[ APPLICATION highlight ]

Analysis of Poly(styrene) Standards in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 2 (125Å and 45Å in series), part numbers 186007000 and 186006995, respectively

Temperature: 40 °C

Injection volume: 10 µL

Sample load: 1 mg/mL

Sample: Poly(styrene) standards

µRIU

Minutes

0.00

5.00

10.00

15.00

20.00

25.00

30.00

35.00

40.00

45.00

50.00

1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20

2800

6570

9890

18.2

K

35K

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64660

22

[ APPLICATION highlight ]

Analysis of Poly(dimethyl silicone) in THF using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: THF

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 4 (125Å and 3 x 45Å in series), part numbers 186007000 and 186006995, respectively

Temperature: 40 °C

Injection volume: 10 µL

Flow rate: 0.6 mL/min

Sample load: 0.2% (w/v)

Sample: Poly(dimethyl silicone)

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64711

µRIU

0.00

0.30

0.60

0.90

1.20

Minutes5.60 7.00 8.40 9.80 11.20

23

[ APPLICATION highlight ]

Analysis of Poly(dimethyl siloxane) in Toluene using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: Toluene

Detection: ACQUITY RI

Column: ACQUITY APC™ XT 4.6 x 150 mm x 3 (125Å and 2 x 45Å in series), part numbers 186007000 and 186006995, respectively

Temperature: 60 °C

Injection volume: 10 µL

Flow rate: 1 mL/min

Sample load: 0.2% (w/v)

Sample: Poly(dimethyl siloxane)

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64712

µRIU

0.00

2.80

5.60

8.40

11.20

Minutes

2.60 3.90 5.20 6.50 7.80

24

[ APPLICATION highlight ]

Aqueous Analysis of Pullulans using ACQUITY APC Columns

LC CONDITIONS System: ACQUITY® Advanced Polymer Chromatography® (APC™)

Eluent: 100 mM sodium nitrate in 80:20 water/acetonitrile

Detection: ACQUITY RI

Column: ACQUITY APC™ AQ 4.6 x 150 mm, 200Å, part number 186006985

Temperature: 40 °C

Injection volume: 20 µL

Sample load: 1 mg/mL

Sample: Pullulan standards

-2.00

0.00 2.00 4.00 6.00 8.00

10.00 12.00 14.00 16.00 18.00 20.00

1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20

107K

47K

21K

9600

6100

µRIU

Minutes

© 2016 Waters Corporation. Waters, ACQUITY, Advanced Polymer Chromatography, and The Science of What’s Possible are registered trademarks of Waters Corporation. APC and ACQUITY APC are trademarks of Waters Corporation. WA64661

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©2016 Waters Corporation. Produced in the U.S.A.October 2016 720004649EN TC-PDF