accelerating antibody discovery - spt labtech · srs tower – 212, 2nd floor sector 31, 14/5...
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mirrorball®
accelerating antibody discovery
get in touchTTP Labtech Ltd
Melbourn Science Park Melbourn Hertfordshire SG8 6EE United Kingdom
+44 (0)1763 [email protected]
TTP Labtech Inc
One Kendall Square Suite B2303 Cambridge MA 02139-1594 United States
+1 (617) 494 [email protected]
TTP Labtech India Private Limited
SRS Tower – 212, 2nd Floor Sector 31, 14/5 Mathura Road Faridabad (NCR Delhi) 121003 India
meetmirrorball®
efficient
your reach
antibodydiscovery
is within
TTP Labtech’s mirrorball fluorescence cytometer revolutionises the productivity of antibody discovery.Simple and economical workflows decrease hands-on time, remove throughput barriers and alleviate budget constraints to make light work of hit identification and characterisation.
Confidently progress lead candidates on time using this reliable and versatile solution.
economicalproductivesimple
reliable versatile
innovationefficiencydelivering
through process
is TTP Labtech’s mirrorball a flow cytometer, or ELISA reader?
Neither, mirrorball uses TTP Labtech’s industry-proven laser scanning fluorescence cytometry to provide HTS-friendly workflows that deliver gold standard data quality.
no-wash screening protocolA simple two-step addition process is all that is required for bead or cell-based homogeneous immunoassays.
mirrorball versus ELISA mirrorball versus flow cytometry
no-wash protocols minimise hands-on time for cell- and bead-based immunoassays
multiplex up to 5 targets to save costs and timein-situ adherent cell analysis eliminates cell suspension protocol step
conserve precious sample (10 µL / test) for downstream hit characterisation
significantly decreased operational and maintenance costs
hit characterisation through image-based functional assays
non-fluidics approach: no delays from system clogging; no potential carry-over
whole well images permit visual confirmation of results for assured decision-making
automation-friendly workflows increase throughput capacity
faster plate read times: 12 mins / plate
incubateread in 12 mins
add10 µL of sample, 10 µL of cells or beads and detection reagent to each well
deliver decision-making data within budget and on time
mirrorball is suitable for hybridoma, phage display, B-cell cloning, antibody fragments, peptides, scFv and Fab fragment screening.
don’t compromise data integrity with mirrorball:
choose the convenience of a bead-based approach where appropriate
preserve the epitopes of cell-surface antigens with cell-based screens
image adherent, or suspension cells in-situ no-fluidics approach:
– low affinity and fast koff interaction detection
– zero carry-over between wells
– no delays due to system clogging
increase productivity with mirrorball:
streamlined no-wash assay protocols multiplex: combine hit identification with non-selective and non-specific counter-screens
reliable walk-away automation for multi-plate screening
work to budget with mirrorball:
economical use of precious sample and reagents low cell and bead requirements / well zero daily / routine maintenance and running costs
work the way you want to within
fluorescence
negati
veposi
tive
work the way you want towithin lead optimisation
and characterisation
mirrorball offers great versatility for performing a wide range of downstream lead optimisation and characterisation tests.progress lead candidates confidently:
species cross-reactivity
competition assays
isotyping
Kd / Bmax determination
functional applications:
– cell-based assays:
– cell health, cell cycle, proliferation, cell signalling, antibody internalisation, virus neutralisation
– bead-based soluble protein assays:
– cytokines, hormones, growth factors
Virus neutralisation assay: whole well fluorescent images from mirrorball showing nuclei (FL1, blue) and virus infected cells (FL-2, green).
neutralising antibody
antibody screening
mirrorball’s Cellista software is powerful yet approachable
For antibody screening, getting started could not be simpler: choose from pre-loaded assay templates and start acquiring data.
On-the-fly analysis gives you decision-making data in just 12 minutes per 96-, 384- or 1,536-well microplate.
Capitalising on mirrorball’s image-based approach Cellista offers great versatility to address a wide variety of functional applications. Cellista extracts multi-parameter data from individual beads, cells or plaques.
development teams will love:
the removal of adoption barriers normally associated with image analysis
hit identification through easy gating of histograms and scatter plots
assured decision-making with whole well images that provide visual confirmation of results
intensity and morphological output measurements
no requirement for specialist operators or intensive training
in just12minutes
click to results
Cellistasoftware:
mirrorball®
main applicationtarget-based biologics screening
laser excitationchoice of 405, 488 or 640 nm
detectionphotomultiplier tube (simultaneous)
fluorescence detection2, 4 or 5 channels plus laser scatter
scan area whole well as standard
standard data format CSV file
plate compatibility 96, 384 and 1,536
scan and analysis time12 minutes per plate (irrespective of well density)
TTP Labtech strives to offer customers the very best in application and instrument support services.
We offer easy set-up and ongoing support from the very first meeting. To further enhance your productivity, assay development and technology transfer services are available upon request. TTP Labtech instruments typically have a life cycle in excess of 10 years, proving a great return on investment.
an effortless route to automation
For no-fuss automation, mirrorball can be supplied with a fully-integrated Thermo Scientific Orbitor with capacity for 80 plates. mirrorball can also be integrated with automation workcells from many major vendors including:
PAA HighRes Biosolutions Wako Automation Agilent Technologies CyBio Beckman Coulter
the end of training is the beginning ofour partnership
sol-R coded beads:make light work of ELISA
watch your costs go down and your productivity go up
TTP Labtech’s sol-R coded beads represent a range of screening reagents for multiplexed assays. Designed for use with mirrorball, they offer a powerful alternative to ELISA.
simple no-wash protocols
productive up to 5 codes for multiplexing
economical screening friendly price point
reliable consistent binding capacity across codes
versatile immobilise whole proteins, fragments or antibody
for antibody screening: enhance productivity through the combination of target specificity and selectivity binding protocols into one assay.
for soluble protein determination: enhance productivity through quantifying multiple proteins in one immunoassay.
available sol-R kits
Any combination of codes 1-5 may be brought together into sol-R bead kits. Kits are optimised for 384-well plates, but protocols for 96-well and 1,536-well plates are also available. TTP Labtech’s sol-R beads may be purchased in volumes for dispensing into 1, 5, 20, 50 and 100 assay plates.
sol-R™ microplate
screening
clarity for image-based immunoassay and phenotypic
TTP Labtech’s sol-R microplate is an affordable, high-quality 384-well plate that has been designed to optimise your mirrorball® or acumen® fluorescence cytometer. It is equally compatible with microscope-based high content imaging devices.
Engineered to ensure confidence in your data, the sol-R plate offers:
optimised design for reliable focus and well positioning
plate flatness and rigidity, without dimples
<3% CVs across the microplate in QC tests
high-grade optical-quality film for clarity
available in sterile and TC treated (cat #3056 0407), or non-sterile and non-TC treated (cat #3056 0404) formats
mirrorball®
accelerating antibody discovery
get in touchTTP Labtech Ltd
Melbourn Science Park Melbourn Hertfordshire SG8 6EE United Kingdom
+44 (0)1763 [email protected]
TTP Labtech Inc
One Kendall Square Suite B2303 Cambridge MA 02139-1594 United States
+1 (617) 494 [email protected]
TTP Labtech India Private Limited
SRS Tower – 212, 2nd Floor Sector 31, 14/5 Mathura Road Faridabad (NCR Delhi) 121003 India