Introduction/Microscope Intro/Cell Basics/Cell Division

A215 Lab

PowerPoint Introductions

•  PDFs of intros can be downloaded from the Lab website’s “Lab Resources” page: http://www.indiana.edu/~anat215/lab/resources.htm

What is Histology?

•  Histology=Microscopic Anatomy – Learn what makes up the different tissues

in the body – Learn how different tissues relate to

structure and function (or malfunction)

Types of Microscopy

•  Electron Microscopy – Powerful magnifying technique used to

visualize intracellular structures – Up to 105x

Magnification Demo (Pin Head)

x33

Magnification Demo (Pin Head)

x100

Magnification Demo (Pin Head)

x250

Magnification Demo (Pin Head)

x1000

Magnification Demo (Pin Head)

x5000

Magnification Demo (Pin Head)

x30,000

Types of Microscopy •  Electron Microscopy

–  Powerful magnifying technique used to visualize intracellular structures

–  Up to 105x

•  Light Microscopy –  Thin section of preserved tissue is cut and placed

on a slide and stained –  4x-1000x

•  in A215, however, you will magnify at most about 400x (40x magnification of virtual microscope objective lens x 10x lens in eyepiece)

•  You need to multiply the number shown on the VM by 10 to find the actual magnification

Types of sectioning

Cross Section Longitudinal Oblique

Muscle

Section? Section? Section?

Cell Basics

Cell Membrane

Nucleus

Cytoplasm

Nucleolus

Cell Basics

•  Not all cells look the same

•  Cells can vary in terms of visible organelles and shape

PDFs of A215 lab electron micrographs can be downloaded using the link at the bottom of the “Lab Resources” webpage

Cell Organelles

Rough Endoplasmic

Reticulum

Cell Organelles

Mitochondria

  Centrioles   9 sets of

triplets

Microvilli

Cilia

Type of sectioning?

Section?

Section?

Cilia 9 doublets

+2

Cell Division

Prophase “Prepare”

Metaphase “Meet”

Anaphase “Apart”

Telophase “Two”

Donor Introduction •  Donors are people who freely donated

their bodies for education •  Male and Female Donors (no longer

referred to here as cadavers) •  New donors re-introduced every Fall •  Prosection done by medical students •  Remains are cremated and returned to

families •  PLEASE SHOW THEM

APPROPRIATE RESPECT

What phase?

What phase?

What phase?

What phase?