To To+24h

Give me !Give me !Give me !

Discover the video on line on www.anios.com

, is better!

Since 1997, Laboratoires ANIOS have led the way in o�ering solutions for pre-treatment of instruments before high-level disinfection, with their first triple-enzyme detergent, ANIOS DL TRI-ENZYMATIQUE.

The fruit of several months' research, ANIOSYME SYNERGY 5 reflects the development of high-performance detergents, with a new synergic combination of 5 enzymes (PROTEASE, LIPASE, AMYLASE, CELLULASE and MANNANASE) and new controlled-foam surfactants.

Eco-formulation with outstanding detergent properties on all types of soil, for all applications (machine, soaking, ultrasonic bath), in all types of water, ANIOSYME SYNERGY 5 o�ers the security of a reliable product and the guarantee of comfortable use.

Pre-treatment of instrumentation before sterilisation:

It's a must!

Pre-treatment of medical-surgical instruments

and medical devices is essential to facilitate

the disinfection stage.

The use of an enzymatic detergent

optimises disinfection, and avoids drying of soil and organic matter (blood, biological

fluid, etc.) and the development of biofilm.

The detergents consist of tensioactive

molecules which hydrate, emulsify,

solubilise, disperse, etc. the soil to be

removed. The enzymes combine with the action of the tensioactive molecules.

EDITORIAL

EFFICACY

E F F I C ACY

PROVEN

ECONOMICAL ECONOMICAL > Dosage: From 0.05% depending on the soil levelDosage: From 0.05% depending on the soil level

and cleaning method usedand cleaning method used

MULTI APPLICATIONMULTI APPLICATION> High detergent power in static bath, ultrasonic bath High detergent power in static bath, ultrasonic bath

and machine

> Non-foaming product for use in washer-disinfectorsNon-foaming product for use in washer-disinfectors

> Proven compatibility with all types of ANIOSProven compatibility with all types of ANIOShigh-level disinfectants high-level disinfectants (STERANIOS 2%, OPASTER, ANIOXYDE 1000, ANIOSEPT ACTIVANIOXYDE 1000, ANIOSEPT ACTIV)ANIOXYDE 1000, ANIOSEPT ACTIV)ANIOXYDE 1000, ANIOSEPT ACTIV

ULTRASONIC BATHAIW*

FASTFAST> E�ective after 1 minute in instrument washer/disinfector,E�ective after 1 minute in instrument washer/disinfector,E�ective after 1 minute in instrument washer/disinfector,

ultrasonic bath and soakingultrasonic bath and soakingultrasonic bath and soaking

EFFECTIVEEFFECTIVE> On all types of soilOn all types of soil

> In all qualities of waterIn all qualities of water

SAFESAFE> ANIOSAFE formulationANIOSAFE formulation

> Foam control: Safe for user and equipmentFoam control: Safe for user and equipment

> Product not classed as dangerous for humans Product not classed as dangerous for humans or the environment when diluted for useor the environment when diluted for use

> > Compatible with all types of materials

A UNIQUEPRODUCT...

THE SYNERGYOF 5 ENZYMES FOR...The characteristic feature of the enzymes is their specificity of action on substrates: as soil is very diverse, ANIOSYME SYNERGY 5 with its 5 enzymes, provides a very wide range of performance on the soil ingredients.

Amylasebreaks down sugars

Cellulaseis specific

to cellulose

Lipaseacts on fats

and lipids

Mannanaseacts on complex

substrates (polysaccharides)

Proteasebreaks down proteins

into amino acids

All enzymes are proteins, and so subject to possible intrinsic break-down by proteases. The ANIOSYME SYNERGY 5 formulation faces this challenge with its latest-generation balanced five-enzyme compound. To illustrate, this study demonstrates the stability and e�cacy of mannanase (and the 5 enzymes) 24 hours after dilution of the product.

Stability of enzymes at a dilution of 0.5% proven for 24 h after dilution

> Example: MANNANASE

Innovative development of a stabilised five-enzyme compound, combined with a pair of so-called controlled-foam surfactants. This original combination ensures a wide range of performance and proven strength of action. Completed by the addition of a biodegradable sequestrant, ANIOSYME SYNERGY 5 acts on all soil types in both soft and hard water.

A SYNERGY OF DETERGENT AGENTS

ENZYMATICSTABILITY

In the case of accidental massive contamination, ANIOSYME SYNERGY 5 demonstrates outstanding bacteriostatic and fungistatic performance.

E�ect of preservation of ANIOSYME SYNERGY 5in case of accidental contamination

MICROBIOLOGICALASSURANCE

Temperature, water quality and mechanical action have a great influence on detergents’ foaming properties. ANIOSYME SYNERGY 5’s ultimate generation of tensioactive guaranties solution clarity and foam control in various situations.ANIOSYME SYNERGY 5 is to be used in dynamic system with no overproduction of foam (AIW, ultrasonic tray) and in static bath.

FOAM CONTROL

2,25E+06

2,00E+06

1,75E+06

1,50E+06

1,25E+06

1,00E+06

7,50E+05

5,00E+05

5,00E+05

2,50E+05

0,00

Microbiological monitoring after massive contamination

Mic

robi

al c

ount

in t

he in

ocul

ated

pro

duct

To 28 days14 days

Pseudomonas aeruginosa ATCC 9027

Staphylococcus aureus ATCC 6538

Candida albicans ATCC 10231

Aspergillus niger ATCC 16404

Substrate

Substrate+

Mannanase

Peace of mind for sta� and patients.

...HIGH-QUALITYFORMULATION

MANNANASEPROTEASE

Electrophoresis on polyacrylamide gel,revelation with Coomassie blue solution

Thin-layer chromatography,revelation with a thymol ethanol solution

in sulphuric acid medium.

Human albumin serum is a protein present in the blood and on soiled instruments. By proteolysis, albumin is broken down into smaller peptides and easily hydrosoluble amino acids.

Mannans are sugars present in many foods and potentially present in the digestive tract. Beta mannanase, chosen specifically, hydrolyses these compounds and facilitates their removal during instrument cleaning. Mannanase, amylase and protease act together on glycoproteins. Lipases contribute to hydrolysis of lipoproteins or liposaccharides...

Appearance of a food additivewithout and with the presence of cellulase

Solubilising power of the enzyme

Drop in viscosity of a solution extractedfrom plant fibres in the presence of mannanase

from 1 minute of contact

This e�cacy is also shown for each of the 5 enzymes:

WITHOUT WITH550

500

450

400

350

300

2500 10 20 30 40 50 60Time (in minutes)

Vis

cosi

ty (

mPa

.s)

Mannanase 0.1%

Substrate:Humanalbuminserum

Substrate+

Protease

Substrate(1,4-ß-d mannan)

Substrate+

Mannanase

According to all methods

SPARKLING INSTRUMENTS!

At 45°C, result after 1 minute at 0.3% in ultrasonic bath

What is cleaning/degreasing power?Cleaning/degreasing power is a methodology developed by Laboratoires ANIOS to evaluate the capacityof a detergent product to remove residual proteins in a complex matrix.

CLEANING DEGREASING POWER

A matchless result... ...in all water qualities

The quality of the water used for dilution is very variable depending on geographical area.The hardness of the water used (calcium and magnesium content) reduces detergent perfor-mance. ANIOSYME SYNERGY 5 keeps all its cleaning properties, whatever the quality of your tap water (tested from 0 to over 40°TH).

100,00

90,00

80,00

70,00

60,00

50,00

40,00

30,00

20,00

10,00

0,00

ANIOSYME SYNERGY 5 diluted with:

Clea

ning

/deg

reas

ing

pow

er v

alue

s (i

n %

)

0,5% | 3minutes 0,5% | 5minutes 0,5% | 10minutes 0,5% | 15minutes

Tap water 40°THSoftened water

Blood represents the reference inert soil on the instrumentation. The cleaning/degreasing power of ANIOSYME SYNERGY 5 is greater than the product X tested under the same conditions.

100,00

90,00

80,00

70,00

60,00

50,00

40,00

30,00

20,00

10,00

0,00Clea

ning

/deg

reas

ing

pow

er v

alue

s (e

n %

)

0,5% | 3minutes 0,5% | 5minutes 0,5% | 10minutes 0,5% | 15minutes

Product X ANIOSYME SYNERGY 5

Proven e�cacy according to all methods

...HIGH-PERFORMANCE DETERGENCY

...ECO FORMULATION

> Limpid solution up to 55°C and non foaming:No risk of injury when collecting the instruments

> Formula with no VOC (volatile organic compounds): No risk of emanations

> No ELV (Exposure Limit Value) or MEV (Mean Exposure Value)

> Neutral pH at the dilution of use

FOR HUMANS

> Active at low concentration: Low product consumption

> Easy to rinse: Low water consumption

> Acts at low temperature: Low energy consumption

> New-generation easily biodegradable chelating agent

> Does not contain EDTA or NTA

> High level of biodegradability (over 99.3% calculated on the organic part of the formulation)

> Not classed as dangerous for the environment

FOR THE ENVIRONMENT

> Fast: Short product / material contact time

> Easy to rinse

> Comprehensive material compatibility

Guaranteed durability of the equipment!

FOR THE EQUIPMENT

Stainless steelAcrylonitrile butadiene styrene (ABS)AG3 aluminiumSynthetic rubberCorian®Viton® and EPDM sealsLatexNeoprene®NitrilePolyamide (PA)Polyvinyl chloride (PVC)High-density polyethylene (HDPE)Methyl polymethacrylate (MPMA)Polysulfone (PSU)Polyurethane (PU)SiliconeTeflon®Titanium

PROVEN EFFICACY ON BIOFILM

After 5 minutes of contact, ANIOSYME SYNERGY 5 demonstrates its capacity to remove the sugars and also proteins coming from the biofilm.

Conc

entr

atio

n of

sug

ars

in μ

g/cm

Detergent power of ANIOSYME SYNERGY 5 against sugars in a biofilm 72 h old (P. aeruginosa)

Initial quantity of sugar in the biofilm

1

Residual quantity of sugar after 5 min. of contact of the product in the biofilm

2

70

60

50

40

30

20

10

0Hard water Product X

1 21 2 1 21 2 1 21 2

...

Pavé du moulin - 59260 Lille-Hellemmes - FRANCETél : (+33) 3 20 67 67 67 - Fax : (+33) 3 20 05 27 48www.anios.com

- EX

2235

- V

ersi

on A

- D

ange

rous

pro

duct

s, re

spec

t the

pre

caut

ions

for u

se –

Non

con

tract

ual p

ictu

res

- Sh

utte

rsto

ck.

PROVEN PROVEN PROVEN SOAKING BATH

EFFICACY

EF F IC ACY

PROVEN

PROVEN COMPATIBILITY WITH

*Automatic Instrument Washer

To To+24h

Give me !

Discover the video on line on www.anios.com

, is better!

Since 1997, Laboratoires ANIOS have led the way in o�ering solutions for pre-treatment of instruments before high-level disinfection, with their first triple-enzyme detergent, ANIOS DL TRI-ENZYMATIQUE.

The fruit of several months' research, ANIOSYME SYNERGY 5 reflects the development of high-performance detergents, with a new synergic combination of 5 enzymes (PROTEASE, LIPASE, AMYLASE, CELLULASE and MANNANASE) and new controlled-foam surfactants.

Eco-formulation with outstanding detergent properties on all types of soil, for all applications (machine, soaking, ultrasonic bath), in all types of water, ANIOSYME SYNERGY 5 o�ers the security of a reliable product and the guarantee of comfortable use.

Pre-treatment of instrumentation before sterilisation:

It's a must!

Pre-treatment of medical-surgical instruments

and medical devices is essential to facilitate

the disinfection stage.

The use of an enzymatic detergent

optimises disinfection, and avoids drying of soil and organic matter (blood, biological

fluid, etc.) and the development of biofilm.

The detergents consist of tensioactive

molecules which hydrate, emulsify,

solubilise, disperse, etc. the soil to be

removed. The enzymes combine with the action of the tensioactive molecules.

EDITORIAL

EFFICACY

E F F I C ACY

PROVEN

ECONOMICAL ECONOMICAL

MULTI APPLICATIONMULTI APPLICATION

ULTRASONIC BATHAIW*

FASTFAST

EFFECTIVEEFFECTIVE

SAFESAFE

A UNIQUEPRODUCT...

THE SYNERGYOF 5 ENZYMES FOR...The characteristic feature of the enzymes is their specificity of action on substrates: as soil is very diverse, ANIOSYME SYNERGY 5 with its 5 enzymes, provides a very wide range of performance on the soil ingredients.

Amylasebreaks down sugars

Cellulaseis specific

to cellulose

Lipaseacts on fats

and lipids

Mannanaseacts on complex

substrates (polysaccharides)

Proteasebreaks down proteins

into amino acids

All enzymes are proteins, and so subject to possible intrinsic break-down by proteases. The ANIOSYME SYNERGY 5 formulation faces this challenge with its latest-generation balanced five-enzyme compound. To illustrate, this study demonstrates the stability and e�cacy of mannanase (and the 5 enzymes) 24 hours after dilution of the product.

Stability of enzymes at a dilution of 0.5% proven for 24 h after dilution

> Example: MANNANASE

Innovative development of a stabilised five-enzyme compound, combined with a pair of so-called controlled-foam surfactants. This original combination ensures a wide range of performance and proven strength of action. Completed by the addition of a biodegradable sequestrant, ANIOSYME SYNERGY 5 acts on all soil types in both soft and hard water.

A SYNERGY OF DETERGENT AGENTS

ENZYMATICSTABILITY

In the case of accidental massive contamination, ANIOSYME SYNERGY 5 demonstrates outstanding bacteriostatic and fungistatic performance.

E�ect of preservation of ANIOSYME SYNERGY 5in case of accidental contamination

MICROBIOLOGICALASSURANCE

Temperature, water quality and mechanical action have a great influence on detergents’ foaming properties. ANIOSYME SYNERGY 5’s ultimate generation of tensioactive guaranties solution clarity and foam control in various situations.ANIOSYME SYNERGY 5 is to be used in dynamic system with no overproduction of foam (AIW, ultrasonic tray) and in static bath.

FOAM CONTROL

2,25E+06

2,00E+06

1,75E+06

1,50E+06

1,25E+06

1,00E+06

7,50E+05

5,00E+05

5,00E+05

2,50E+05

0,00

Microbiological monitoring after massive contamination

Mic

robi

al c

ount

in t

he in

ocul

ated

pro

duct

To 28 days14 days

Pseudomonas aeruginosa ATCC 9027

Staphylococcus aureus ATCC 6538

Candida albicans ATCC 10231

Aspergillus niger ATCC 16404

Substrate

Substrate+

Mannanase

Peace of mind for sta� and patients.

...HIGH-QUALITYFORMULATION

MANNANASEPROTEASE

Electrophoresis on polyacrylamide gel,revelation with Coomassie blue solution

Thin-layer chromatography,revelation with a thymol ethanol solution

in sulphuric acid medium.

Human albumin serum is a protein present in the blood and on soiled instruments. By proteolysis, albumin is broken down into smaller peptides and easily hydrosoluble amino acids.

Mannans are sugars present in many foods and potentially present in the digestive tract. Beta mannanase, chosen specifically, hydrolyses these compounds and facilitates their removal during instrument cleaning. Mannanase, amylase and protease act together on glycoproteins. Lipases contribute to hydrolysis of lipoproteins or liposaccharides...

Appearance of a food additivewithout and with the presence of cellulase

Solubilising power of the enzyme

Drop in viscosity of a solution extractedfrom plant fibres in the presence of mannanase

from 1 minute of contact

This e�cacy is also shown for each of the 5 enzymes:

WITHOUT WITH550

500

450

400

350

300

2500 10 20 30 40 50 60Time (in minutes)

Vis

cosi

ty (

mPa

.s)

Mannanase 0.1%

Substrate:Humanalbuminserum

Substrate+

Protease

Substrate(1,4-ß-d mannan)

Substrate+

Mannanase

According to all methods

SPARKLING INSTRUMENTS!

At 45°C, result after 1 minute at 0.3% in ultrasonic bath

What is cleaning/degreasing power?Cleaning/degreasing power is a methodology developed by Laboratoires ANIOS to evaluate the capacityof a detergent product to remove residual proteins in a complex matrix.

CLEANING DEGREASING POWER

A matchless result... ...in all water qualities

The quality of the water used for dilution is very variable depending on geographical area.The hardness of the water used (calcium and magnesium content) reduces detergent perfor-mance. ANIOSYME SYNERGY 5 keeps all its cleaning properties, whatever the quality of your tap water (tested from 0 to over 40°TH).

100,00

90,00

80,00

70,00

60,00

50,00

40,00

30,00

20,00

10,00

0,00

ANIOSYME SYNERGY 5 diluted with:

Clea

ning

/deg

reas

ing

pow

er v

alue

s (i

n %

)

0,5% | 3minutes 0,5% | 5minutes 0,5% | 10minutes 0,5% | 15minutes

Tap water 40°THSoftened water

Blood represents the reference inert soil on the instrumentation. The cleaning/degreasing power of ANIOSYME SYNERGY 5 is greater than the product X tested under the same conditions.

100,00

90,00

80,00

70,00

60,00

50,00

40,00

30,00

20,00

10,00

0,00Clea

ning

/deg

reas

ing

pow

er v

alue

s (e

n %

)

0,5% | 3minutes 0,5% | 5minutes 0,5% | 10minutes 0,5% | 15minutes

Product X ANIOSYME SYNERGY 5

Proven e�cacy according to all methods> Cleaning/degreasing power on di�erent types of soil Cleaning/degreasing power on di�erent types of soil

(grease and blood) after soaking(grease and blood) after soaking> On so-called "German" soil described by EN 15883 On so-called "German" soil described by EN 15883

standard in ultrasonic bathstandard in ultrasonic bath

> Washing monitors such as TOSI®, Browne STF load check®,Wash-checks ® in washer-disinfectors

> ATP monitoring

...HIGH-PERFORMANCE DETERGENCY

...ECO FORMULATION

> Limpid solution up to 55°C and non foaming:No risk of injury when collecting the instruments

> Formula with no VOC (volatile organic compounds): No risk of emanations

> No ELV (Exposure Limit Value) or MEV (Mean Exposure Value)

> Neutral pH at the dilution of use

FOR HUMANS

> Active at low concentration: Low product consumption

> Easy to rinse: Low water consumption

> Acts at low temperature: Low energy consumption

> New-generation easily biodegradable chelating agent

> Does not contain EDTA or NTA

> High level of biodegradability (over 99.3% calculated on the organic part of the formulation)

> Not classed as dangerous for the environment

FOR THE ENVIRONMENT

> Fast: Short product / material contact time

> Easy to rinse

> Comprehensive material compatibility

Guaranteed durability of the equipment!

FOR THE EQUIPMENT

Stainless steelAcrylonitrile butadiene styrene (ABS)AG3 aluminiumSynthetic rubberCorian®Viton® and EPDM sealsLatexNeoprene®NitrilePolyamide (PA)Polyvinyl chloride (PVC)High-density polyethylene (HDPE)Methyl polymethacrylate (MPMA)Polysulfone (PSU)Polyurethane (PU)SiliconeTeflon®Titanium

PROVEN EFFICACY ON BIOFILM

After 5 minutes of contact, ANIOSYME SYNERGY 5 demonstrates its capacity to remove the sugars and also proteins coming from the biofilm.

Conc

entr

atio

n of

sug

ars

in μ

g/cm

Detergent power of ANIOSYME SYNERGY 5 against sugars in a biofilm 72 h old (P. aeruginosa)

Initial quantity of sugar in the biofilm

1

Residual quantity of sugar after 5 min. of contact of the product in the biofilm

2

70

60

50

40

30

20

10

0Hard water Product X

1 21 2 1 21 2 1 21 2

...

- EX

2235

- V

ersi

on A

- D

ange

rous

pro

duct

s, re

spec

t the

pre

caut

ions

for u

se –

Non

con

tract

ual p

ictu

res

- Sh

utte

rsto

ck.

PROVEN PROVEN PROVEN SOAKING BATH

EFFICACY

EF F IC ACY

PROVEN

PROVEN COMPATIBILITY WITH

*Automatic Instrument Washer