a robust and sensitive synthetic sensor to monitor the transcriptional output of the cytokinin...
TRANSCRIPT
![Page 1: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah](https://reader036.vdocuments.site/reader036/viewer/2022082611/56649ed25503460f94be26b2/html5/thumbnails/1.jpg)
A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network
in Planta
by Evelyne Zürcher, Deborah Tavor-Deslex, Dmytro Lituiev, Katalin Enkerli, Paul T. Tarr, and Bruno Müller
PlantphysiolVolume 161(3):1066-1075
February 28, 2013
©2013 by American Society of Plant Biologists
![Page 2: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah](https://reader036.vdocuments.site/reader036/viewer/2022082611/56649ed25503460f94be26b2/html5/thumbnails/2.jpg)
Optimization of TCS. A, A concatemer of 24 repeats of the 5′-(A/G)GAT(C/T)T-3′ binding motif caused the strongest cytokinin-dependent induction of a LUC reporter in transient transfection
assays.
Zürcher E et al. Plantphysiol 2013;161:1066-1075
©2013 by American Society of Plant Biologists
![Page 3: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah](https://reader036.vdocuments.site/reader036/viewer/2022082611/56649ed25503460f94be26b2/html5/thumbnails/3.jpg)
Sensitivity and specificity of TCSn::LUC in transient transfection assays.
Zürcher E et al. Plantphysiol 2013;161:1066-1075
©2013 by American Society of Plant Biologists
![Page 4: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah](https://reader036.vdocuments.site/reader036/viewer/2022082611/56649ed25503460f94be26b2/html5/thumbnails/4.jpg)
TCSn::GFP in the seedling.
Zürcher E et al. Plantphysiol 2013;161:1066-1075
©2013 by American Society of Plant Biologists
![Page 5: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah](https://reader036.vdocuments.site/reader036/viewer/2022082611/56649ed25503460f94be26b2/html5/thumbnails/5.jpg)
TCSn::GFP expression in different developmental contexts.
Zürcher E et al. Plantphysiol 2013;161:1066-1075
©2013 by American Society of Plant Biologists