a robust and sensitive synthetic sensor to monitor the transcriptional output of the cytokinin...

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A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah Tavor-Deslex, Dmytro Lituiev, Katalin Enkerli, Paul T. Tarr, and Bruno Müller Plantphysiol Volume 161(3):1066-1075 February 28, 2013 ©2013 by American Society of Plant Biologists

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Page 1: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah

A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network

in Planta

by Evelyne Zürcher, Deborah Tavor-Deslex, Dmytro Lituiev, Katalin Enkerli, Paul T. Tarr, and Bruno Müller

PlantphysiolVolume 161(3):1066-1075

February 28, 2013

©2013 by American Society of Plant Biologists

Page 2: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah

Optimization of TCS. A, A concatemer of 24 repeats of the 5′-(A/G)GAT(C/T)T-3′ binding motif caused the strongest cytokinin-dependent induction of a LUC reporter in transient transfection

assays.

Zürcher E et al. Plantphysiol 2013;161:1066-1075

©2013 by American Society of Plant Biologists

Page 3: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah

Sensitivity and specificity of TCSn::LUC in transient transfection assays.

Zürcher E et al. Plantphysiol 2013;161:1066-1075

©2013 by American Society of Plant Biologists

Page 4: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah

TCSn::GFP in the seedling.

Zürcher E et al. Plantphysiol 2013;161:1066-1075

©2013 by American Society of Plant Biologists

Page 5: A Robust and Sensitive Synthetic Sensor to Monitor the Transcriptional Output of the Cytokinin Signaling Network in Planta by Evelyne Zürcher, Deborah

TCSn::GFP expression in different developmental contexts.

Zürcher E et al. Plantphysiol 2013;161:1066-1075

©2013 by American Society of Plant Biologists