a report on interactive meet of the network partners cum ... · pdf filedr. r. a. hazarika dr....

1

A report on interactive meet of the Network partners cum training on Advanced Animal Disease Diagnosis And Service Management Centres held on 25th – 27th

February 2015 organized by Core Lab-I, CVSc, Khanapara__

Members Attended:

Dr R N GoswamiDr A ChakrabortyDr. D. D. KulkarniDr. Ashwin A Raut

Dr. S. C. YadavDr. Nitin VirmaniDr. Sanjay BaruaDr. R K Vaid

Dr. D. HemadriDr. M. R. Gajendragad Dr. K. P. SureshDr. S. S. PatilDr. Rajes wari ShomeDr B.R. Shome

Dr.(Ms) P. PassahDr.(Ms) I. WarjriDr. Ng. Ibotombi Singh

DeanDirector of Research (Vety)DirectorSr Scientist

Pr. ScientistPr. ScientistPr. ScientistPr. Scientist

Pr. ScientistPr. Scientist Sr. ScientistSr. ScientistPr. ScientistPr. Scientist

Add Director ManipurDIO, MeghalayaO/C DIL

FVSc, AAU, KhanaparaAAU, KhanaparaICAR-NISHAD- BhopalICAR-NISHAD- Bhopal

ICAR-NRCE - HISARICAR-NRCE- HISARVTCC-NRCE-HISSARVTCC-NRCE-HISSAR

NIVEDI, BengaluruNIVEDI, BengaluruNIVEDI, BengaluruNIVEDI, BengaluruNIVEDI, BangaluruNIVEDI, Bangaluru

Meghalaya, ShillongMeghalaya, ShillongImphol, Manipur

Dr(Ms). Esther RalteDr. Semsem BhutiaDr. Asis BhatacharyaDr. Logo Bage

Vety Officer (DI)Joint Director (R)

Assoc Professor,

Aizawl, MizoramSikkim , GangtokTripura Vety College AgartalaDIL , Nirjuli, Arunachal Pradesh

Dr. Tapan Kumar DuttaDr. T. RahmanDr. B.K. SarmahDr. Probodh BorahDr. P. C. SarmahDr. R. A. HazarikaDr. Arnab SenDr. Samu DasDr. K. PuroDr. Samprity BaruahDr S K DasDr N N Barman

Assoc Prof.Prof. & HeadDDR (i/c)Prof. & Head Prof & Head Prof & HeadProf & HeadScientistScientistR.A. PI cum Professor & HeadProfessor

C.V.Sc, CAU, AizawlPathology, CVSC, KhanaparaCVSc, KhanaparaAnimal Biotech, CVSc, KhanaparaParasitology, CVSc, KhanaparaDept. Of Public health, CVSc, Animal health, ICAR BarapaniICAR, Barapani ICAR, Barapani

ICAR, BarapaniDepartment of Microbiolgy, CVSc, Khanapara

2

The DBT Center in the North East on “Advanced Animal Disease Diagnosis and Services management” is a tripartite concept involving (1) Research Institutes and Agricultural Universities located in the NE Region, (2) National Animal Science Institutes and (3) State Animal Husbandry Departments (SAHD) of NE Region. The project will operate for five years. All these Institutions will coordinate in mission to develop regional core competitiveness to strengthen diagnostic capabilities of endemic, ubiquitous and transboundary diseases of animal and poultry, development of sensitive disease diagnostic protocols and user friendly diagnostic kits, data base management and training to the regional and state level scientists and technical manpower. The network project was officially launched by Dr Harsh Vardhan, Honourable Minister of Science and Technology, Earth Sciences on 17th February at Assam Agricultural University, Khanapara Campus. The prime objective of the project is to bring national scientistsworking in three partner national institute, scientists of regional institutes and stake holders of eight animal husbandry departments in a single plate form. Accordingly an interactive meet was organised for all three partners in the Core laboratory I of the College of Veterinary Science, Khanapara from 25th – 27th February 2015. The meet was initiated on 25th February 2015 by Dr A Chakraborty, Project coordinator of the lead centre cum Director of Research (Vety), Assam Agricultural University, Khanapara. Dr S K Das, Principal Investigator, Core Lab I welcomed all participants including HODs of the host faculty to this unique meet. Dean, Faculty of Vety Science, Khanapara, addressed all delegates and apprised about ongoing activities of the Faculty. It was followed by presentations on progress so far made by the network partners, highlights on priority areas to interact with different partners and points to be raised before funding agency.

A. Progress of the projects : All core lab partners (core labs I,II,III) as well as national partners (NIVEDI earlier PD_ADMAS; NIHSAD earlier HSADL; NRCE/VTCC) have almost completed recruitment of project staffs and contractual labourers. They were trained to handle diagnostic facilities as well as official formalities in respective centre. However, NRCE/VTCC informed that the amount earmarked for contractual workers is far below than state wages and recruitment of contractual worker at such consolidated pay will be violation of state labour law of Haryana Govt.

Core lab I informed that necessary formalities for construction of Laboratory arein advanced stage. Similarly all participating centres informed about the satisfactory progress made in respect of purchase of chemical, glass wares and instruments/equipments etc. With published protocols limited genomics and proteomic diagnostics are standardized at National and NER partner institutes. Besides, conventional diagnostics are either procured or developed to give diagnostic services at field units. National partners are in the process of developing point of care disease diagnostic tools. A contact list is prepared with updated e mail IDs and phone numbers of all involving members of the network partners and circulated to all for regular interaction. Core labs of NER initiated dialogues with state Animal Husbandry stake holders and identified four field disease diagnostic units in each states of North Eastern region. Core

3

Lab I also informed that training programmes were also conducted in two phases for field level officers on setting up of diagnostic laboratory, collection of samples from animals/birds, epidemiological data, performing of post-mortem examination, handling & dispatch of samples to core-labs etc. A close interaction among core lab partners and SAHD officials was held to identify their actual requirements and modalities to achieve the targets of the project. All national and regional network partner institutes initiated research programmes as per mandate of the respective sub-projects. Project staffs of core labs visited fields in their assigned jurisdictions to collect samples from Pigs, cattle, goats, duck, Chicken regularly. Diagnostic services were also made available to the farmers, pet owners and wild life workers. Sera samples and microbial isolates are preserved in respective core labs to dispatch to the National Repository Centre.

B. i. Priority areas of Core Labs for interacting with Network partners:

The very objective of association of national labs in this project is to strengthen core lab diagnostic facilities at par with national diagnostic laboratories so as to provide timely diagnosis of important animal and avian diseases in this region of the country. With that idea, all three Core labs focused areas of interaction with NIVEDI on development of uniform formats for collection of epidemiological data, field samples, use of sample codes, sample registry, access to existing disease data base, procedure of entryof disease data, sharing of SOPs and available diagnostic kits. NIHSAD needs to train on handling of BSL facilities, TADs samples, analyzing, reporting and sharing of protocols and available kits to diagnose emerging diseases at core lab level. NRCE/VTCC will facilitate sample repository guidelines including preservation protocols (virus, bacteria, fungus, sera), modalities of sample dispatch and transportation, sharing of SOPs, kits, standard Ag, Ab, amplicons for JE, Influenza , haemoprotozoa. Core lab-I raised certain issues to establish linkage with state stake holders for effective and continuous receipt of samples from field.

ii. Priority areas of National labs for interacting with NE partners:

NIVEDI developed genus specific PCR and multiplex PCR for detection of pathogenic bacteria as well as PCR for detection of MRSA, MR-CoNS, ESBL etc.-The SOPs for brucellosis diagnostic tests, for detection of CSFV are also ready to share with NE core labs. Centre can provide user friendly diagnostic kits and technical know-how of diagnosis of Brucellosis and MRSA and CSF, PRRS, TTV. They insisted mutual sharing of the materials (antigens, reagents and sera samples) to strengthen the laboratories and will visit field and core labs to improve lab facilities. Centre can devote for establishment of common SOPs for of field labs and core labs.

4

In respect of NRCE/VTCC, institute can involve to create facilities in core labs to diagnose influenza A (Equine and Swine), Japanese encephalitis/Westnile fever, trypanosomiasis. Related proteomic and genomic protocols are ready to share with NE labs.

NIHSAD can help in development of diagnostics for High-risk Emerging Animal/Zoonotic diseases. Centre will extend all support to train Human Resource in the area of High-risk Emerging Disease Diagnosis and Biosafety. Necessary protocols, kits developed by the centre will be shared with the core labs. Dr. D D Kulkarni, Director, felt very much to develop cell culture CSF vaccine immediately and same should be made available at any cost for the pig growers of the region.

iii. Priority areas for interacting with SAHDs :

State Animal Husbandry partners expressed about shortage of manpowers, paucity of funds and requirement of users’ friendly diagnostics for screening of animal and avian diseases. The state Govt officers shared their experience of difficulties in collection of blood, sera samples etc. They were of the view that for collection of any samples, some incentives in the form of Medicines, feeds. mineral mixtures is a pre requisite and without which collection of any form of information/ sample will not be possible.

Core labs raised certain pertinent points to establish successful linkages with state partners.

i). A nodal team should be constituted to liaise with Director taking state PI as member.

ii). Disease sampling zones/grids should be identified in consultation with respective core labs and survey works can be initiated nearby field units.

iii). State stake holders should take initiative to obtain permission for interstate transfer of samples.

iv). Modalities can be derived to collect samples/information from border areas.

v). State PIs should finalize the mode of transfer of money to state directorate and same should be communicated to the respective Project co-ordinator immediately.

vi). A high level team from University may contact Commissioners and Directors of the eight state A.H. & Veterinary Department to ensure compliance and proper implementation of the project at the state level. Project coordinator of the Lead Centre, Dr A Chakraborty will take the initiative in this regard shortly.

C. Points to be raised for consideration of the Funding Agency:

All network partners identified following common as well as individual issues to be forwarded to the DBT for their consideration and positive action.

5

1. The title of the project that was proposed in the proposal as “ ADVANCED ANIMAL DISEASE DIAGNOSIS AND SERVICE MANAGEMENT CENTERS (AADSMC)” fits to be the title of the project as per objectives of the project. Hence it should be retained instead of the title mentioned in the Sanctioned letter [ Advanced Animal Diagnostics and Services on Animal Health and Diseases (ADSAHD)]. A fresh circular may be issued so as to use in future official correspondence.

2. For smooth functioning of the project, the contribution of all scientists working either in regional or national Institutes needs to be recognized. So NER BPMC may be approached to include them as Co-PI. (Separate sheet is enclosed)

3. Establishment of livestock disease scenario in NER as well as strengthening of competency of human resource in diagnosing diseases is the main focused areas of the project. Therefore, more funds should be allotted in travel head as well as in contingency head for out sourcing of field samples.

4. Regular supply of diagnostic kits, chemicals/plastic-glass wares are necessary to the field units. Therefore, more funds should be allotted under consumable head to all centres.

5. Necessary guidelines should be issued and make arrangements to release fund for payment of salary to the RA/SRF/JRF in enhanced rate in view of the recent order appeared in the circular of DBT. In this regard the wages of Lab Assistant/Office Assistant should be enhanced and HRA should be provided.

6. The wages sanctioned in the project for state like Haryana are too low for appointment of contractual labourer/skilled labourer. Their engagement in this time bound project is utmost necessary. Hence fund may be enhanced.

7. To develop infrastructure facilities at field diagnostic units, fund should be released to all state centres by second year itself. Again, the state wise allocation of budgets to the core lab II needs to be reviewed, as the core lab II has been entrusted with three states whereas budgets have been given for two states only.

8. For NIVEDI, due to escalation of price of instruments one unit of FPA and BD phoenix could not be procured. Allotment of more funds requires to procure the same.

9. Since project will continue for 5 years, NIHSAD, Bhopal proposes dividing of training budget for 5 years instead of 3 years.

10. NIHSAD, Bhopal and NRCE, Hisar need permission for procuring of another instrument instead of approved equipment within the same budget.

11. All Project co-ordinators (National/Core labs/SAHDs) will write to the Advisor,DBT, NER BPMC briefing about their issues with a copy to the Project coordinator, Lead Centre, AAU, Khanapara to take up issues at Delhi.

It was followed by scientific presentations on assigned topic By Dr N N Barman, Dr. D. D. Kulkarni, Dr. Ashwin A Raut, Dr. D. Hemadri, Dr. M. R. Gajendragad , Dr. K. P. Suresh, Dr. S. S. Patil, Dr. S. C. Yadav, Dr. Nitin Virmani, Dr. Sanjay Barua, Dr. R K Vaid, Dr. Rajeswari Shome, Dr B.R. Shome. First day meet was ended with vote of thanks by Dr S K Das, PI of the Core Lab I.

6

Recommendations of the tripartite meet as Road Map of the Project and Action to be taken :

After a threadbare discussion and interaction among the members, a consensus was reached to prepare a road map with common minimum programme so that a visible progress can be achieved. Identified areas are as follows:

1. Prioritization of livestock species to be covered in the first phase: Based on the priority of the North Eastern Region, diagnostic facilities will be created in core labs with the help of national laboratories for diseases of pigs, goats and ducks. Intensive investigation on diseases affecting these species will be carried out in NE states during the first two years. However, diagnostic activities on other animals will also continue at the same time.

2. Infectious agents to be studied : Infectious agents affecting pigs, goats and ducks are grouped as endemic and emerging/re-emerging. Animal species

Infectious agent

Endemic Emerging/Re-emerging

Pigs

Bacteria Salmonella, Pasteurella, Erysipelas, Carbapenem resistant E Coli. Anthrax; Clostridium, Brucella

Oedema disease, Carbapenem resistant E Coli.

Virus CSFV, PPV, RV, JEV, Rota, PRRS Circo virus, PEDV, Swine Influenza, Nipah virus, TGEV, Swine Pox, TTV

Parasites Nematodes ( Ascaris, oesophagostomum, stephanurus, gnathostoma, capillaria etc), trematodes ( Fasciola, opisthorchis, artyfechinostomum, gastrodiscoides), Larval cestode ( Cysticercus cellulosae, hydatids) , Haemoprotozoa, coccidia

Goats

Bacteria Pasteurella, Clostridia, Listeria, Mycobacterium paratuberculosis, Mycoplasma, Anthrax, Brucella

Virus PPR, Orf, BTV, Goat pox, Para influenza,

Nipah,Encephalities causing virus

Parasites Trematodes (Fasciola, Amphistome, Schistosomes ), GI Nematodes (Haemonchus, mecistocirrus, oesophagostomum, bunostomum, cooperia), Cestodes (Moniezia, stilesia, avitellina, Taenia & Echinococcus), coccidian and haemoparasites (Babesia, theileria, anaplasma).

Bacteria Pasteurella, Salmonella, Botulism, Mycotoxin

Virus DPV DV Hepatities, AI

7

Ducks Parasites Trematodes (Echinostoma, tracheophilus, prosthogonimus), Nematodes ( Ascaridia,

heterakis, porocaecum, tetramers), Cestode (Raillietina, Fibbriaria, hymenolepis),

coccidi and haemoparasite.

Each national partner and NE core lab will take responsibility to develop diagnostics and confirm diseases prevalent in eight states. Action to be taken by the respective centre as assigned below:

Action to be taken by NIVEDI :

Animal species Endemic & emerging/re-emerging infectious agents

PigsBacteria Brucella, AMRVirus CSFV, PRRS,TTV

Goats Bacteria Brucella, AMR, Mycoplasma

Action to be taken by NIHSAD:

Animal species Endemic & emerging/re-emerging infectious agentsPigs Virus Nipah, Ebola, Influenza A virus, PRRSGoats *Virus CCHF, NSD, RVF

Ducks Virus Influenza A virus

Action to be taken by NRCE/VTCC :


PigsBacteria Salmonella, Pasteurella, Erysipelas Virus RV, JEV, Swine Influenza, Parasites Trypanosoma (rest of the component to be

taken up at Khanapara and NRCE will assist for other parasites)

Goats

Virus Para influenza, Encephalities causing virus

Parasites Trypanosome (rest of the component to be taken up at Khanapara and NRCE will assist for other parasites)

Action to be taken by NRC on pigs:


PigsBacteria Salmonella, Pasteurella, BrucellaVirus CSFV, RV, Circo, Parvo virus

8

Action to be taken by Core Lab I :

Animal species

Endemic & emerging/re-emerging infectious agents

Pigs

Bacteria Salmonella, Pasteurella, Brucella, Erysipelas, AMR, Anthrax; clostridium ,Oedema disease

Virus CSFV, PPV, RV,PRRS, Swine Pox.Parasites, vector

Nematodes ( Ascaris, oesophagostomum, stephanurus, gnathostoma,capillaria etc), trematodes ( Fasciola, opisthorchis, artyfechinostomum, gastrodiscoides), Larval cestode ( Cysticercus cellulosae, hydatids) , Haemoprotozoa, coccidian, vector

Goats Bacteria Brucella, AMR, Pasteurella, Clostridia, Listeria, Mycobacterium paratuberculosis, Anthrax

Virus OrfParasites, vector

Trematodes (Fasciola, Amphistome, Schistosomes ), GI Nematodes (Haemonchus, mecistocirrus, oesophagostomum, bunostomum, cooperia), Cestodes (Moniezia, stilesia, avitellina, Taenia &Echinococcus), coccidian and haemoparasites (Babesia, theileria, anaplasma). vector

Ducks

Bacteria Pasteurella, Salmonella, Botulism, Mycotoxin.Virus DPVParasites Trematodes (Echinostoma, tracheophilus, prosthogonimus),

Nematodes ( Ascaridia, heterakis, porocaecum, tetramers), Cestode (Raillietina, Fibbriaria, hymenolepis), coccidi and haemoparasite.

Action to be taken by Core Lab II :

Animal species

Endemic & emerging/re-emerging infectious agents

Pigs

Bacteria Salmonella, Erysipelas, Brucella, AMR,Virus CSFV, JEV,

Circo virus, PRRSParasites, vector


Goats Bacteria Brucella, AMR, ListeriaVirus NipahParasites, vector

Trematodes (Fasciola, Amphistome, Schistosomes ), GI Nematodes (Haemonchus, mecistocirrus, oesophagostomum, bunostomum, cooperia), Cestodes (Moniezia, stilesia, avitellina, Taenia & Echinococcus), coccidian and haemoparasites (Babesia, theileria, anaplasma), vector

Ducks

Bacteria Pasteurella, Salmonella, Afla toxinVirus DPVParasites Trematodes (Echinostoma, tracheophilus, prosthogonimus),

Nematodes ( Ascaridia, heterakis, porocaecum, tetramers), Cestode (Raillietina, Fibbriaria, hymenolepis), coccidia and haemoparasite.

9

Action to be taken by Core Lab III :


Pigs

Bacteria Salmonella, Pasteurella, Brucella, AMR, E Coli., Oedema disease

Virus PRRS Parasites, vector


Goats Bacteria Brucella, AMR, Pasteurella, Listeria, Mycobacterium paratuberculosis

Parasites,Vector

Trematodes (Fasciola, Amphistome, Schistosomes ), GI Nematodes (Haemonchus, mecistocirrus, oesophagostomum, bunostomum, cooperia), Cestodes (Moniezia, stilesia, avitellina, Taenia & Echinococcus), coccidian and haemoparasites (Babesia, theileria, anaplasma), vector

Ducks

Bacteria Pasteurella, Salmonella, Afla toxinParasites Trematodes (Echinostoma, tracheophilus, prosthogonimus),

Nematodes ( Ascaridia, heterakis, porocaecum, tetramers), Cestode (Raillietina, Fibbriaria, hymenolepis), coccidi and

haemoparasite.

*Goat diseases on PPR, BTV, Goat pox will be covered from 3rd year onwards as there are DBT funded ongoing projects on these agents.

3. Strengthening of Human Resource : The prime objective of the project is to developdisease diagnostic competency in NER Labs through training human resources. Accordingly certain topics are identified and decided to carry out training programmes at national as well as at core lab levels within second year of the project as per tentative time schedule indicated below:

Training Module

Training Details Tentative date

Action to be taken by

Trainees

Passive survey

Survey strategy, data entry, record keeping

22-24 April, 2015

NE core Lab-I/NIVEDI

Core lab personnels, state Animal Husbandry personnels

Active survey

Sampling strategy, collection of samples, preservation & dispatching

10

Training carried out at National labs, necessary TA/DA for SAHD officers should be arranged by the host institutes and at core labs fund should be arranged by core lab. However, matter should be taken up at DBT level to obtain permission for payment of TA & DA from Training & also allotment of more fund under head Training.

4. Development of Laboratory capability at Core Labs :

a. Protocols standardized/developed as per OIE guidelines and diagnostic kits/reagents/ positive control/negative control available at national labs as well as in other core labs should be shared with NE core labs. Feed back of test results can be used as validation of the tests. Such protocols can be finalised as common SOPs for use in the diagnosis of diseases in NE states. Necessary MTA should be completed by respective National partners so as to dispatch kits/reagents to respective centres within 2 months.

TADs samplings

Lab, sample handing, advanced diagnosis of TADs

April, 2015 NIHSAD Core lab personnels, SAHD personnels

Field diagnosis

Basic Microbial, pathological, parasitic methods – Gross diagnosis, Microscopical, Serodiagosis,

June, 2015 NE core Labs I/II/III

state Animal Husbandry

personnels

Advanced diagnosis

Molecular diagnostic protocols (OIE/Inhouse), Serodiagnosis, isolation techniques July, 2015

NIVEDI/

NIHSAD/NRCE/VTCC/NRC pigs

NE core Labs/NIVEDI/HSADL/NRCE/VTCC/NRC pigs

Disease mapping

GIS application, analysis and interpretation

May, 2015

NIVEDI/

NE core Labs/

NIVEDI

Core lab personals, state Animal Husbandry personals

Software management & Data analysis

Data analysis, data interpretation & presentation

Reposition Methods on reposition & storage, Sample coding, record keeping, preservation, dispatch.

August,2015

NRCE/ VTCC

11

Agents Type of SOPs/kits available

Action to be taken by SOPs/kits provided to

PRRSVPCR protocol NIHSAD, Core lab II,

NIVEDICore lab I & III

Indirect ELISA kits NISAD Core labs I,II,III

JEV PCR protocol NRCE, Core lab II Core lab I & III

HI protocol/kits NRCE Core labs I,II,III

AIV Ag –Ab kits NIHSAD Core labs I,II,III, Field labs

NDV PCR protocol Core lab I Core labs II,III, Field labsHI

protocol/reagents

DPV PCR protocol Core lab I Core lab II, III

i-ELISA protocol

RV PAGE, PCR protocol

Core lab I, NRCE Core labs II,III

Isolation protocol Core lab I

Swine pox

PCR protocol Core lab I Core labs II,III

Fowl pox PCR protocol Core lab I Core labs II,III

Swine influenza

PCR protocol NRCE Core labs I,II,III

PPR, BTV, Goat Pox

PCR protocol NIVEDI Core labs I,II,III

AMR PCR protocol NIVEDI, Core lab III Core labs I,II

Brucella PCR protocol NIVEDI Core labs I,II,III, Field labsAb kits

Clostridia PCR protocol Core lab I Core labsII,III, National labIsolation protocol

Listeria PCR protocol Core lab II Core labs I, III

Isolation protocol

Trypenosoma

PCR protocol NRCE Core labs I,II,III, Fieldlabs

ELISA protocol

Babesia, Theileria

PCR protocol Core lab I Core labs II,III, National lab

12

b. Common protocols used by Core lab I, II and NIVEDI for detection of CSFV by PCR and Ag-Ab detection tests and isolation of the virus will be validated by ring tests. Consensus protocols in the light of EU SOPs will be finalized and developed as common SOP for use in diagnosis of CSFV in NE states. Action will be taken by Core lab I and NIVEDI.

c. For development of SOPs to detect PPV, Circo virus, PEDV, TGEV, Nipah virus, TTV, Duck hepatitis virus, Erysipelas, Oedema disease, cysticarcus, hydatid, coenurus, qPCR for Theileria, Babesia, , Aflatoxin, Anthrax should be initiated by identified centres and necessary clinical/tissue samples should be provided from field by core labs for standardization of tests.

5. Development of Laboratory Capability at Field units :a. A diagnostic laboratory manual will be prepared covering necessary formats,

protocols for preparation of various reagents, diagnosis of microbial, parasitic, toxicological diseases and clinic-pathological, pathological investigations etc. Action to be taken by scientists from NIVEDI, VTCC, Core labs I, II and III (2 months).

b. All 3 core labs should arrange training for field staffs for collection of disease data, samples and despatch of samples to the core labs. Necessary reagents, plastic and glass wares, preservation/transport media should be provided. To begin withdiagnostic activities quick and handy diagnostic kits/reagents should be arranged.

6. Formats to be prepared :

Type of formats Action to be taken (One month)

Passive survey format, outbreak survey, format for Sero- survey, routine parasitic survey, check list for collection of sample.

VTCC ; NIVEDI; core labs

Uniform sample code for tissue, sera, isolates will be developed.

VTCC ; NIVEDI; core labs

Guidelines & formats for reporting of investigation results for bacteria/Virus/serologcal/ parasitic/toxic/pathological investigations.

NRCE

Sample entry & sample registry formats NIVEDI;VTCC ; core labs

Disease data base/ Accessibility to NADRES. NIVEDI

Development of Website Core labs I, II and NIVEDI

Monthly report format from field unit NIVEDI

13

7. Sampling Strategy :a. Initially for 6 months survey will be confined in and around field diagnostic units of

the respective state as there are no funds available in the 1st year for SAHDs.Information on number of villages, type of species available, No of animal herd/unit/bird unit, Av animals per herd/unit, Av. birds per unit will be collected and intimated to NIVEDI through core Labs. Action to be taken by PIs of SAHD and Core lab I (31st March 2015).

b. NIVEDI will identify the village and framed the sample number, frequency for sera samples, clinical samples, faecal samples in routine survey as well as during outbreak. Action to be taken by NIVEDI (April 2015).

c. Samples will be collected as stated below jointly by project staffs and Field lab incharges. All consumables/ medicines/vaccines to be used in field dung collection of samples should be arranged by core labs.

Animal/bird Type of sample No. of sample/state

Pig

Sera sample (3month-1 yr age group) 200 (50 /F. Lab)

Blood smears (Haemoprotozoa) 100 (25 / F. Lab)

Vector survey

Nasal swabs 100 (25 / F. Lab)

Faecal samples (herd/unit basis of young, grower, adult)

400 (100 / F. Lab)

Organs in dead carcass As per the mortality/ outbreak

Goat



Vector survey

Nasal swabs 200 (50 / F. Lab)

Faecal samples (herd/unit basis of young, grower, adult)

400 (100 / F. Lab)

Organs in dead carcass As per the mortality/ outbreak

Duck



Tracheal swabs 40 (10 / F. Lab)

Faecal droopings (unit basis of young, grower, adult)

200 (50 / F. Lab)

Organs in dead carcass Dead/ ailing bird

d. The participation of NERDDL is crucial and agreement regarding sample sharing, diagnostic protocol implementation and disease informatics need to be

e. Samples collected in field will be dispatched to core labs through project staffs, messenger from field, through courier service /overnight service. Expenditure will be borne by respective core lab. Between core labs samples will be transported by project staffs/courier service at every month. Samples from core labs to national labs will be dispatched at every 3/6months by courier/messenger. Emergency samples will be sent by messenger. All cost involve in dispatching samples to national labs should be arranged by respective national lab. samples / dispatch to national lab will be arranged by PIs of SAHD and National labs.

f. A nodal liaison representative at the National Institute level needs to be fcommunicated to all implementing partners.

g. There should be communication of fortnightly reports regarding work progress under this project to Core lab I in order to implement a real time monitoring and evaluate progress achieved by the impleme

The participation of NERDDL is crucial and agreement regarding sample sharing, diagnostic protocol implementation and disease informatics need to be

Samples collected in field will be dispatched to core labs through project staffs, messenger from field, through courier service /overnight service. Expenditure will be borne by respective core lab. Between core labs samples will be transported by

staffs/courier service at every month. Samples from core labs to national labs will be dispatched at every 3/6months by courier/messenger. Emergency samples will be sent by messenger. All cost involve in dispatching samples to national labs should

ged by respective national lab. Official permission for interstate transport of samples / dispatch to national lab will be arranged by PIs of SAHD and National labs.

A nodal liaison representative at the National Institute level needs to be fcommunicated to all implementing partners.There should be communication of fortnightly reports regarding work progress under this project to Core lab I in order to implement a real time monitoring and evaluate progress achieved by the implementing partners.

14

The participation of NERDDL is crucial and agreement regarding sample sharing, diagnostic protocol implementation and disease informatics need to be worked upon.

Samples collected in field will be dispatched to core labs through project staffs, messenger from field, through courier service /overnight service. Expenditure will be borne by respective core lab. Between core labs samples will be transported by

staffs/courier service at every month. Samples from core labs to national labs will be dispatched at every 3/6months by courier/messenger. Emergency samples will be sent by messenger. All cost involve in dispatching samples to national labs should

Official permission for interstate transport of samples / dispatch to national lab will be arranged by PIs of SAHD and National labs.

A nodal liaison representative at the National Institute level needs to be finalised and

There should be communication of fortnightly reports regarding work progress under this project to Core lab I in order to implement a real time monitoring and evaluate

16

List of Principal Investigator / Co- PIs of the network partners to be notified

Centre name Name, designation, address of PI/CO-PI

NIVEDI

Dr B.R. Shome, Pr. Scientist as CO-PI

Dr. Rajeswari Shome, Pr. Scientist as CO-PI

Dr. S. S. Patil, Sr Scientist as CO-PI

Dr. K. P. Suresh, Sr Scientist as CO-PI

Dr. M. R. Gajendragad , Principal Scientist as CO-PI

Dr. D. Hemadri, Principal Scientist as CO-PI

NIHSAD

Dr Ashwin Ashok Raut, Senior Scientist, as CO-PI

Dr Shashi Bhushan Sudhakar, as CO-PI

Dr Pradeep N Gandhele as CO-PI

Dr K Rajukumar, Senior Scientist, as CO-PI

Dr A K Pateriya, Scientist, as CO-PI

NRCE

Dr B. N. Tripathi, Director, ICAR-NRCE, Project Co-ordinator

Dr Nitin Virmani, Principal Scientist, as CO-PI

Dr S.C. Yadav, Principal Scientist, as CO-PI

Dr B.R. Gulati, Principal Scientist, as CO-PI

Dr Rajender Kumar, National Fellow, as CO-PI

VTCC

Dr Sanjay Barua, Principal Scientist, as CO-PI

Dr R.K.Vaid, Principal Scientist, as CO-PI

Dr B.C.Bera, Scientist, as CO-PI

Dr Taruna Anand, Scientist, as CO-PI

Dr Riyesh, T, Scientist, as CO-PI

NRCE PigDr. S. Rajkhowa, Sr. Scientist, as CO-PI

Dr. Seema Pegu, Scientist, as CO-PI

Core lab I

Dr S K Das, Professor, Microbiology, as PI

Dr N N Barman, Professor, Microbiology, as CO-PI

Dr. D K Deka, Professor, Parasitology, as CO-PI

Dr P Hussain, Asst Professor, Public Health, as CO-PI

17

Dr T Rahman, Professor, Pathology, as CO-PI

Core Lab IIDr. R Laha- Principal Scientist, as CO-PI

Dr. I Shakuntala, Principal scientist, as CO-PI

Dr. Sandeep Ghatak, Senior Scientist, as CO-PI

Dr. K. Puro, Scientist (Senior Scale), as CO-PI

Dr Samir Das (Scientist), as CO-PI

Core Lab III

Dr. Parimal Roychoudhury, Asst. Prof., as CO-PI

Dr. Gunjan Das, Assoc. Prof., as CO-PI

Dr. S. K. Borthakur, Asst. Prof. as CO-PI

Dr. Rahul Singh Arya. Asst. Prof., as CO-PI

Dr. Jagan Mohanarao Gali, Asst. Prof., as CO-PI

a report on interactive meet of the network partners cum ... · pdf filedr. r. a. hazarika dr....

Documents