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Western Plant Diagnostic Network News

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Dear WPDN First Detectors, The brown marmorated stinkbug (BMSB) has continued its inevitable spread and was found in early September in downtown Sacramento CA, just south of the state capitol. It has been present in southern CA south of the Tehachapi Mountains since 2006. Most of Oregon and southern Washington are already infested with this stinkbug. The BMSB is a serious threat to agriculture as well as being a structural nuisance in the fall and winter. There are two very amazing photos of BMSB “aggregating” on a tree in Sacramento. Sudden oak death (SOD) continues to spread both in the natural environment and through the nursery trade. The article on SOD updates the scientific and the regulatory aspects of the pathogen and disease. The feature article is about invasive fruit flies. Dr. Jason Leathers of the California Department of Food and Agriculture describes the most serious exotic fruit flies, biology, modes of entry, and regulatory action taken in response to them.

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Newsletters

Western Plant Diagnostic Network

First Detector News

A Quarterly Pest Update for WPDN First Detectors

Summer 2013 edition, volume 6, number 2

In this Issue

Page 1: Editor’s Note:

Pages 2: BMSB Update

Pages 3 - 4: Sudden Oak Death update Pages 5 - 9: Exotic fruit flies

Contact us at the WPDN Regional Center at UC Davis: Phone: 530 754 2255 Email: rwhoenisch@ucdavis.edu Web: https://wpdn.org Editor: Richard W. Hoenisch @Copyright Regents of the University of California All Rights Reserved

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Western Plant Diagnostic Network News

Brown Marmorated Stink Bug now in Northern California

The brown marmorated stink bug (BMSB), Halyomorpha halys , has been spreading in the western United States. BMSB has finally reached Sacramento, California, where it is established in the neighborhood just south of the state capitol. The first announcement was made on September 12, 2013. The California Department of Food and Agriculture (CDFA) and the Sacramento County agricultural commissioner’s office are investigating and delineating the infestation. The BMSB is a very serious pest of agriculture and horticulture, and a structural nuisance. Please see the WPDN 2010 Fall and 2011 Winter newsletters for detailed descriptions of the BMSB. See the Sacramento Bee and the Wine Business News articles for information on the infestation.

Dr. Tracy Leskey, research entomologist with the USDA – ARS (Agricultural Research Service), working at the Appalachian Fruit Research Station in Kearneysville, WV, is one of the experts on the BMSB. Dr. Leskey and her team have created a series of videos on the various aspects of the BMSB, the BMSB Video Series. These videos will give one an idea of the size, high populations, the damage, and the nuisance associated with the BMSB. Dr. Frank Zalom, entomologist with the University of California, Davis, has a BMSB Power Point presentation. UC Davis Foundation Plant Services newsletter BMSB FPS Newsletter describes the infestation in California. The BMSB in NW Oregon has very good ID photos.

Brown marmorated stink bugs aggregating on a Chinese Pistache

tree at 13th and P Streets in Sacramento CA. The photos were

taken in September 2013.

Photos by Baldo Villegas and courtesy of Chuck Ingels, Sacramento County Farm Advisor

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Western Plant Diagnostic Network News

Sudden Oak Death (SOD) Phytophthora ramorum

Of growing importance in the environment and in the nursery industry, Sudden Oak Death (SOD) is the common name of a disease caused by the oomycete plant pathogen Phytophthora ramorum. The disease kills oak and tanoak and other species of trees and shrubs, even including poison oak. It has had devastating effects on the oak populations in California and Oregon as well as also being present in Europe. Symptoms include bleeding cankers on the tree's trunk and dieback of the foliage, in many cases eventually leading to the death of the tree. The California Oak Mortality Task Force webpage provides extensive information about the organism and its effect on the environment and its mode of spread. P. ramorum is also present in the forest of a small part of Curry County in Oregon. P. ramorum has a large host range and can cause diverse symptoms including trunk cankers, leaf spots, or twig diebacks depending on the host species infected. P. ramorum is also found in nurseries, where it causes ramorum blight characterized by leaf spots and dieback primarily on Rhododendrons, Pieris, Camellias and Viburnums. Ramorum blight generally does not kill the entire plant (although it can), whereas SOD cankers that occur on the trunks of white oaks and tan oaks are often fatal. New research suggests that in tan oak, P. ramorum induces the production of tyloses that plug the xylem and limit water movement up the stem in addition to decaying cambial tissue. Symptoms of the disease and symptoms on nursery plants are found at SOD Diagnosis Questionnaire. P. ramorum has been detected on nursery stock in the western United States and British Columbia and in a smaller number of nurseries in the eastern U.S. More recently, it has been found in a limited number of water ways in association with nurseries in the southeastern U.S. Please see this site for details Sudden Oak Death 5th Science Symposium To prevent infection of the oak forests in the eastern United States and its spread in the nursery industry, the USDA requires that in CA, OR and WA, each nursery must be inspected and tested and found free from P. ramorum before it is allowed to ship plants out of state. P. ramorum cannot be identified by symptoms alone. Many species of Phytophthora infect nursery plants and produce similar symptoms to P. ramorum, so laboratory analysis is necessary to distinguish it from other Phytophthora

species. Many training opportunities to learn more about P. ramorum are available on line: Sudden Oak Death Education Resources. Nursery Best Management Practices have been developed for growers who want to control all Phytophthora species and to specifically prevent the introduction of P. ramorum into their nursery growing grounds.

Borys Tkacz USDA-Forest Service

Dead tanoaks on Mt. Tamalpais CA

USDA-Forest Service

Figure 1Dying Douglas fir

Photo by John Bienapfl

Dead Tanoaks in Marin Co. CA SOD symptoms on Douglas fir

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Western Plant Diagnostic Network News

Sudden Oak Death Ecology

Professor David Rizzo, a plant pathologist and mycologist in the Department of Plant Pathology at UC Davis, gave a presentation at a first detector training session at the UC Davis Arboretum on August 2, 2013. Dr. Rizzo is one of the lead scientists with the California Oak Mortality Task Force. It has been found that where Umbellularia californica (bay laurel, California bay laurel, Oregon myrtle) grows, P. ramorum thrives. U. californica acts as a Typhoid Mary for P. ramorum, having foliar infections but not dying from the pathogen. This is one of the main sources of the pathogen in the natural landscape. Where there is no bay laurel, there is no natural spread of the disease. Map 1 is the range of SOD, and Map 2 is the range of bay laurel.

The subgenus of oaks is another factor in susceptibility to or tolerance of the pathogen. The white oak group is very susceptible to the pathogen, while the red oak group is very tolerant of the pathogen. Is it a matter of genetics? This is another piece of the Sudden Oak Death puzzle.

The origin of the pathogen is still under investigation. At first it was thought to have come from the Netherlands or Germany in rhododendron, azalea, or camellia nursery material. Through genetic analysis this was disproven. The next hypothesis was that it came from Japan or somewhere in Asia, but this has also been discounted. P. ramorum was first discovered in California in 1995 when large numbers of tanoaks died mysteriously in Marin and Santa Cruz counties. It is possible that P. ramorum is native to the United States. Infection rates could have previously been at a low level, but changes in the environment caused a change to the population structure. Alternatively, the symptoms of P. ramorum may have been mistaken for that of other pathogens. the dynamics of this new pathogen and disease.

Range of Umbellularia californica

Map 2

Bay laurel leaves infected with P.

ramorum

Bay laurel – Umbellularia californica

Distribution of P. ramorum in

forests

Map 1

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Western Plant Diagnostic Network News

Exotic fruit flies ( Diptera: Tephritidae ) such as Mediterranean fruit fly ( Ceratitis capitata ) and oriental fruit fly (Bactrocera dorsalis group ) are among the most serious threats to California’s $43 billion agricultural industry. These invasive pests are highly polyphagous, capable of breeding in a very wide variety of fruits and vegetables. Adult female flies pierce the skin of fruits and vegetables and lay eggs inside. The eggs hatch and develop into maggots, which feed on the pulp, rendering the produce unfit for consumption. Since fruit flies are internal feeders, they can be transported long distances by people carrying fruits and vegetables. The establishment of these pests would have significant repercussions including a significant increase in pesticide use and the loss of export markets.

To exclude exotic fruit flies and other pests from the United States, USDA, APHIS cooperates with Customs and Border Protection (CBP) officials to inspect incoming plant products, including illicit arrivals. The California Department of Food and Agriculture (CDFA) also inspects incoming vehicles for illicit fruits and vegetables at land border stations in California. Additionally, USDA, APHIS conducts surveillance of markets for illegal fruits and other plant products, and California County Agricultural Commissioners conduct inspections at high risk introduction facilities in their counties, such as package distribution centers (e.g., FedEx), nurseries, and markets.

Nevertheless, the expanding volume of international commerce offers exotic fruit flies ample opportunities to make regular incursions into California. The vast majority of these incursions occur in urban areas of southern California and the San Francisco Bay area, where the major international ports of entry are located. In order to detect these incursions, the USDA, APHIS and CDFA Cooperative fruit fly program manages approximately 90,000 fruit fly traps throughout California. This detection trapping system has regularly shown itself to be sufficiently sensitive to detect populations while they are small enough to be eradicated using methods which are most effective against small, isolated populations. In addition, once an exotic fruit fly is captured in a trap, much denser arrays of traps are used to confirm that a population exists in the area and, if so, to determine its distribution and estimate its size. Following control activities, similarly dense arrays of traps are used to provide a high degree of assurance that a viable population no longer exists in the area.

The female oriental fruit fly is fertilized by the male, lays her eggs on the fruit, the eggs hatch into maggots

that feed on the pulp and destroy the fruit.

Oriental fruit fly

Photos by Okinawa Prefectural Fruit Fly Eradication

Photo by Scott Bauer USDA

Maggots feeding Female ovipositing Female ovipositing

Exotic Fruit Flies in California A feature article by Jason Leathers, Ph.D.

Primary State Entomologist, California Department of Food and Agriculture

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Western Plant Diagnostic Network News

As of September 18, a total of 80 oriental fruit flies have been trapped this year in California. All of these have been found in the urban areas of southern California and the San Francisco Bay area. Two areas have been quarantined following the confirmation of breeding populations of flies. These areas include parts of the cities of Anaheim (Orange County), Cerritos, and Artesia (Los Angeles County). A total of 43 adult oriental fruit flies were trapped in Anaheim. Here the flies were focused around a secluded residential property that maintained a fruit orchard. During the first visit to that property, inspectors hand-collected an additional 50 adult oriental fruit flies. In addition, nine larvae were found in peaches at a neighboring property. Only one fly has been trapped since August 23. The second area quarantined for oriental fruit fly is in the cities of Cerritos and Artesia. In this area a total of 19 flies were trapped between July 9 and August 14. The other 18 oriental fruit flies were trapped in the Orange County cities of Santa Ana, Fullerton, Seal Beach, and Garden Grove, the Los Angeles County cities of Los Angeles, Glendale, Placentia, and Long Beach, the San Diego County city of San Diego, the Alameda County city of Pleasanton, and the Santa Clara County cities of Cupertino and San Jose. Four species of exotic fruit fly have been detected in California in 2013. These include three species of the genus Bactrocera, which are native to Asia. These are oriental fruit fly, peach fruit fly (Bactrocera zonata ), and guava fruit fly (Bactrocera correcta ). The fourth species is Caribbean fruit fly (Anastrepha suspensa ), which is native to the Caribbean.

A total of 13 peach fruit flies have been trapped this year. These include 3 flies trapped in the cities of Chino and Chino Hills (San Bernardino County), 6 flies in the cities of Fairfield and Suisun City (Solano County), and 4 flies in the Alameda County cities of Fremont, Union City, and San Lorenzo.

A total of 6 guava fruit flies have been trapped this year. These include 5 flies trapped in the Santa Clara County cities of Cupertino and San Jose and 1 fly trapped in Garden Grove (Orange County).

The single Caribbean fruit fly was trapped in Los Angeles in the vicinity of Los Angeles International Airport (LAX).

High-density delimitation trapping will continue around each of these fly detections well into 2014, to allow for three life-cycles of the flies to pass based upon degree day models. Once three life-cycles have passed without any fly finds, the density of traps will be returned to detection levels.

Photo by Viwat Wornoayporn IAEA Photo from Russell IPM Photo from “The Fruit Flies”

Peach Fruit Fly

Male (l) and female (r) peach fruit flies Female ovipositing on fruit Maggots feeding on peach

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Western Plant Diagnostic Network News

Adult female Caribbean fruit fly

Photo by FL Division of Plant Industry

Maggots feeding

Photo by Jack Kelly Clark

Photo courtesy of the IAEA Imagebank

Guava Fruit Fly

Photo by Pravakar Padhial

Photo by The Budget Gardener

Photo courtesy of the Food & Fertilizer Technology Center

Female (l) & male (r) guava fruit fly Female ovipositing on guava Maggots feeding on guava

Caribbean Fruit Fly

Female ovipositing on guava

Photo by FL Division of Plant Industry

Other Problem Flies

Photo by Joaquin Alves Gaspar

Photo by pesticide guy

Male Med fly Female ovipositing on citrus Maggots feeding on orange

Mediterranean fruit fly Ceratitis capitata

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Western Plant Diagnostic Network News

Unlike other vinegar flies that occur in the Western US, spotted wing drosophila attacks healthy ripening fruit as well as damaged or split fruit. The female will penetrate the skin of soft-skinned fruit with her large ovipositor and lay eggs just under the skin, creating a small puncture, or “sting,” on the fruit surface. Each sting contains 1 to 3 eggs, and a female can oviposit on many fruit. Multiple larvae within a single fruit are quite possible, because many

Melon fruit fly Bactrocera cucurbitae

Photo by Scott Bauer Photo by Paul Zbrowski

Photo from Russell IPM

Photo by John Davis

Photo by Elizabeth Beers WSU

Photo by Hannah Burrack NCSU

Photo by Bev Gerdeman WSU

Spotted Wing Drosophila Drosophila suzukii

Only the male SWD has spotted wings

Female SWD (no spotted wings)

Male (l) & female (r) on strawberry

Maggots feeding on strawberry

Photo by Hannah Burrack NCSU

Female SWD ovipositing on raspberry

Male melon fruit fly

Female melon fruit fly Maggots feeding on melon

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Western Plant Diagnostic Network News

females might visit the same fruit to oviposit. Once fruit integrity is compromised by SWD’s activities, common vinegar flies also might oviposit in the damaged fruit.

Eggs hatch and maggots develop and feed inside the fruit, causing the flesh to turn brown and soft with sunken areas that can exude fluid on the surface of smooth-skinned fruit such as cherries and blueberries. Damage can provide an entry site for infection by secondary fungal and bacterial pathogens, but this is not always the case.

As Dr. Leathers writes above: “Since fruit flies are internal feeders, they can be transported long distances by people carrying fruits and vegetables. The establishment of these pests would have significant repercussions including a significant increase in pesticide use and the loss of export markets.” As First Detectors, it is important to know and observe quarantines for fresh fruit. Do not send or receive fresh fruit from quarantine areas or across state (and in some cases even county) lines. A good example is fruit exported from Hawaii. Hawaii is infested with the melon fly (1895), the Mediterranean fly (1907), Oriental fly (1944) and the Malaysian fly (1983). Fruit exported to the mainland must be treated to ensure none of these flies infest other areas. Irradiation, vapor heat, and hot water treatments are among the methods used. These methods kill any fly larva within the fruits. See Why We Treat Fruit For Export and Heated Air Blasts Papaya Pests . Customs and Border Patrol guidelines explain the philosophy and biology behind the regulations. “PLEASE – DO YOUR PART TO HELP PROTECT AMERICAN AGRICULTURE! When planning your trip, keep in mind that regulations change frequently around the world, depending on outbreaks of plant and animal diseases. So, whether or not the item in question seems to be one that is permitted, travelers are still responsible for declaring those items and presenting them for inspection upon returning to the United States. DECLARE all agriculture-related products when entering the United States.”

Repeat Offenders

In conversations with the USDA and various state departments of agriculture, invasive fruit flies turn up over and over again in the same locations. Oftentimes friends and families may ship fruit into the US from abroad, or people visiting abroad may bring produce and/or animal products and/or live plants and propagules with them. All of these can harbor fruit flies and other diseases and this is why it is almost always in the same locations. UC Davis has recently published a study: Medfly and other fruit flies entrenched in California, study concludes. The CA Eradication trapping program describes the methods of fruit fly detection and trapping. Fruit fly quarantine areas in CA is found on the CDFA web site. For your state, go to the Pest Tracker site, select States, and then select your state to find your state agriculture contact info and the status of plant diseases, insect pests, invasive weeds, etc.

More about fruit flies