a protein interface
DESCRIPTION
A Protein Interface. Protein Folding. Protein. tRNA. Ribozome. mRNA. http://www.wehi.edu.au/education/wehi-tv/dna/Translation.mov. acetylcholine esterase. Binding Site (Negative). Acetylcholine (positive). http://www.scripps.edu/mb/goodsell/interface/interface_images. - PowerPoint PPT PresentationTRANSCRIPT
A Protein Interface
Protein
Ribozome
tRNA
mRNA
http://www.wehi.edu.au/education/wehi-tv/dna/Translation.mov
Protein Folding
acetylcholine esterase
Acetylcholine(positive)
Binding Site(Negative)
http://www.scripps.edu/mb/goodsell/interface/interface_images
Amino Acid Sequence ofthe enzyme:
acetylcholine esterase1 mfvnqrtrrp ymsvfvlvlg aavicpaygi idrlvvqtss gpirgrstmv qgrevhvfng 61 vpfakppvds lrfkkpvpae pwhgvldatr lppsciqery eyfpgfagee mwnpntnvse 121 dclylniwvp tktrlrhgrg lnfgsndyfq ddddfqrqhq skgglamlvw iygggfmsgt 181 stldiynaei laavgnviva smqyrvgafg flylapying yeedapgnmg mwdqalairw 241 lkenakafgg dpdlitlfge saggssvslh llspvtrgls krgilqsgtl napwshmtae 301 kalqiaegli ddcncnltml kespstvmqc mrnvdaktis vqqwnsysgi lgfpsaptid 361 gvfmtadpmt mlreanlegi dilvgsnrde gtyfllydfi dyfekdaats lprdkfleim 421 ntifnkasep ereaiifqyt gwesgndgyq nqhqvgravg dhfficptne falglterga 481 svhyyyfthr tstslwgewm gvlhgdevey ifgqpmnasl qyrqrerdls rrmvlsvsef 541 artgnpaleg ehwplytren piffifnaeg eddlrgekyg rgpmatscaf wndflprlra 601 wsvpskspcn lleqmsiasv sstmpivvmv vlvliplcaw wwaikknktp phpqviletr 661 afmh
GenBank Sequence
The Amino Acids
David L. Nelson and Michael M. Cox, Lehninger Principles of Biochemistry, 3rd edition, 2000, Worth Publishers, ISBN 1-57259-153-6
Proteins Fold by Twisting Peptide Backbone
http://www.itb.uni-stuttgart.de/training/bioinformatics99
Protein Secondary Structure
Tertiary Structure (3-Dimensional Fold)
• Motif– Simple combination of secondary structures– Appears in different proteins
• Domain– More complex combination of secondary
structures– Has a specific function
Predicting Folding
• Fold predictions are made by minimizing free energy
• Hydrogen Bonds Decrease Free Energy
• Some backbone angles are more stable than others