©2009 Waters Corporation | COMPANY CONFIDENTIAL©2011 Waters Corporation – COMPANY CONFIDENTIAL

A new On-line Solid Phase

extraction system

from Waters

Acquity OSM

Denis Calnan

Clinical/Forensic Business

Specialist (Northern Europe)

Båstad Nordics Symposium 2011

©2009 Waters Corporation | COMPANY CONFIDENTIAL©2011 Waters Corporation – COMPANY CONFIDENTIAL

Agenda

Acquity Online Solid Phase Extraction Manager (OSM)

How was it developed?

Benefits of online SPE

Hardware

• Questions/ Discussion

©2011 Waters Corporation – COMPANY CONFIDENTIAL 3

Introducing Acquity Online SPE Manager (OSM)

“A UPLC®-enabled online solid phase

extraction system”

©2011 Waters Corporation – COMPANY CONFIDENTIAL 4

What is Acquity OSM?

The Acquity OSM

— A device to provide UPLC-enabled online SPE

— OSM (online SPE Manager)

— Part of the Acquity online SPE System

o Acquity Sample Manager

o Acquity Binary Solvent Manager

o Acquity Column Manager – A

o Acquity TQ Detector

(or Xevo TQ MS + TQS)

©2011 Waters Corporation – COMPANY CONFIDENTIAL 5

How did we get here?

Online SPE investigations began in Jan 2006

— Spark Holland Symbiosis Pharma

o Plasma Free Metanephrines

o Urinary Free Cortisol

o Testosterone & TDM

Attractive features

— Processing of biological matrices

— New cartridge for every sample

— Decent results & publications

— Easier than any Waters solution at the time

©2011 Waters Corporation – COMPANY CONFIDENTIAL 6

Undesirable Features of Spark Symbiosis and other HPLC based on-line SPE system

Awkward to deploy in the field

— Sales & support of non-Waters system

Size & Configuration

— Large sample management module

— Limited solvent capacity (mobile phases)

— Lengthy fluidic pathways

— Separate column oven (control?)

HPLC pressures in a UPLC world

©2011 Waters Corporation – COMPANY CONFIDENTIAL 7

What are the attractions of online SPE?

All of the benefits of standard SPE

— Sample clean-up

— Sample concentration

— Automation

Online SPE

— Sample efficiency

o Off-line SPE (plates, cartridges) always loses something

o Online, everything on the cartridge is passed to the column

— Parallel processing of samples

— Multiple Modes of Operation

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The Anatomy of the Acquity OSM

Acquity Sample Manager

• 2 samples trays• Variable injection volume (max 200µL)• (optional sample organizer)

Acquity BinarySolvent Manager

• 4 Solvent Selection• 15,000 psi

Acquity Column Manager

• 4 column capacity

•Modified access characteristics

Acquity Online SPE Manager

Acquity TQ Detector

“A UPLC®-enabled online solid phase

extraction system”

©2011 Waters Corporation – COMPANY CONFIDENTIAL 9

A closer look at Acquity OSM

Left Clamp(Elution)

Right Clamp(Loading)

High Pressure Dispenser (HPD) SPE Solvent

Inlets

Valving to allow switching of

modes

Gripper to move cartridges to & form clamps

2 x 96-well plates/trays of cartridges

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Tray of SPE cartridges

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Amount Of Phospholipids Present In Whole Blood Before And After Extraction Using The OSM

whole blood after extraction

Whole blood before extraction

©2011 Waters Corporation – COMPANY CONFIDENTIAL 12

Ion Suppression For Whole Blood Extracted Using The OSM

Extracted whole blood

50% methanol (aqueous)

©2011 Waters Corporation – COMPANY CONFIDENTIAL 13

IATDMCT 2011 poster

©2011 Waters Corporation – COMPANY CONFIDENTIAL 14

Benefits of Acquity OSM

A single vendor solution with traceability features

— Hardware, chemistries & integrated software

Automated online SPE – all of the benefits of SPE and more

— Removing the human factor

o Time, errors and injury

o Parallel processing & improved time-to-first result

— Sample Clean-up

— Sample Concentration

Consolidation of methods means system flexibility

— Reduction in column changes

— Reduction in mobile phase changes

— Reduction in SPE solvent handling

— Other online systems are dedicated to a particular analysis

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Conclusion

Single cartridge per sample – very low risk of carry-over

Operates in UPLC mode ie, faster runtimes, enhanced

resolution + increased sensitivity

Options of up to 23 solvents for SPE steps

Flexible system with multiple chemistry options will allow

seamless switching between methods

Complete software control within MassLynx

ISO 13485 and EU IVDD 98/79/EC

Currently in Demo labs and ―Seed evaluation‖ sites

Available Q2 2012