a biochip for recording electrophysiological signals of nematodes
DESCRIPTION
A biochip for recording electrophysiological signals of nematodes . Group 13-27 Advisor: Liang Dong Group members: Xuan Qiao Yuncong Chen Yang Cui Ruiyu Chen. Introduction. Overview Project design Related background Project working process. Overview of our project. General view. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/1.jpg)
A biochip for recording electrophysiological signals of nematodes
Group 13-27Advisor: Liang DongGroup members:Xuan QiaoYuncong Chen Yang CuiRuiyu Chen
![Page 2: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/2.jpg)
IntroductionOverviewProject designRelated backgroundProject working process
![Page 3: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/3.jpg)
Overview of our project
General view
Detail structure
Amplifier circuit Testing environment
![Page 4: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/4.jpg)
Concept sketchBiochip device: container as an
environment
Electrodes: electrophysiological signal transform
Amplifier: electrophysiological signal measurement
![Page 5: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/5.jpg)
Testing TargetC. Elegans
![Page 6: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/6.jpg)
Our DesignHigh efficient trappingConvenient electrodes
![Page 7: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/7.jpg)
Our Design30 um gold electrodes 15 um channels
![Page 8: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/8.jpg)
Why C. elegansThe genes of C. elegans nematodes
are similar to those of human beings.
Nematodes are simple, easy to study
Biologists use C. elegans as model system to study toxicology and discover new drugs for human metabolic and degenerative diseases
![Page 9: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/9.jpg)
Why Electrophysiological signals Electrophysiological signals from
C. elegans provide information about the activity of the pharynx (throat) which pumps like a heart during feeding
C. elegans has a distinct set of drug receptors present on its pharynx.
![Page 10: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/10.jpg)
Why biochipLow cost- $30/liter can make a hundred of our devices
High throughput- Once you have the design, it is easy to make hundreds of biochip devices
Simple operation- Biochip devices are easy to use
![Page 11: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/11.jpg)
SchameticAmplifier Design
![Page 12: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/12.jpg)
SimulationResult:
![Page 13: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/13.jpg)
Testing inveriment1.We don’t have the
optical table to eliminate the influence from movement of people
2. When we do the test in Coover, there is no wire box to enclose the testing area to filter the noise in the air
![Page 14: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/14.jpg)
Testing result
2 pA
1000 m s
2 pA
50 m s
The amplitude shows how strong the nematode is pumping.
The frequency shows how frequent the nematode is pumping.
Initial Contraction
Terminal Contraction
![Page 15: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/15.jpg)
Working process
Pick worm Spin the Biochip
Worms in the bio chip
By using the spinning coder, the biochip can get the worms in every channels. This is why we call it “high trapping rate”
![Page 16: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/16.jpg)
Working process
ChannelNematode Electrod
ePharynx
![Page 17: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/17.jpg)
Working process
![Page 18: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/18.jpg)
Challenges in The Project• 15 um channel fabrication
• Gold etching process
• No alignment mark
• Noise reduction
![Page 19: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/19.jpg)
Questions
![Page 20: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/20.jpg)
Back up slides
![Page 21: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/21.jpg)
![Page 22: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/22.jpg)
Constraints & ConsiderationsAll equipment and device need to set up on a experiment table.
Group members should wear lab cloth, gloves and goggles.
Gold etching with strong acid
![Page 23: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/23.jpg)
Our project
![Page 24: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/24.jpg)
Non-functional requirementsdevice fabricationTaking Picture of Worms and
Taking video
Electrodes fabricationDo alignment with microscope
![Page 25: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/25.jpg)
Division of LaborXuan & Yuncong & Yang: device
design, fabrication and Electrodes fabrication
Abhishek & Ruiyu: amplifier design and construction
![Page 26: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/26.jpg)
Potential RisksAlignment for device and
electrodesChemicals, sharps, UV exposure,
wormsBurning of op-amps, resistors,
capacitorsNoisy signalStrong acid in electrodes
fabricationWorms die in culture
![Page 27: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/27.jpg)
MitigationBeing generally diligent will go a
long wayDuctility: most experienced
member will make device to ensure proper thickness
Safety risks: all trained in lab safety, know proper procedures to avoid accidents
Burned components/noisy signal: use of quality components and careful construction
![Page 28: A biochip for recording electrophysiological signals of nematodes](https://reader035.vdocuments.site/reader035/viewer/2022081512/56816128550346895dd080c9/html5/thumbnails/28.jpg)
Market Surveytraditional experiment: 1. trap and test worms one by one2. Expensive and waste time
Our design: 1. trap and test 16 worms at the same time 2. The device is small and cheap