a 198 115 90.5 123 ip: ha wce +++ hdm2 ha-ub flag-htert mg132 - ++ +++ -- + wb: flag wb: hdm2 wb:...
TRANSCRIPT
a
198
115
90.5
1 2 3
IP: HA
WCE
+ + +
Hdm2
HA-Ub
FLAG-hTERT
MG132
- + +
+ + +
- - +
WB: FLAG
WB: Hdm2
WB: FLAG
hTERT-(Ub)n
Supplementary Figure S1. Oh et al.
WB: HA
WB: Hdm2
WB: FLAGWCE
+ + + + + +
MG132
Hdm2
HA-Ub
FLAG-hTERT
1 2 3 4 5 6
hTERT-(Ub)n
- - + - - +
- + + - + +
- +
198
115
90.5
WB: FLAG
IP: FLAG
b
a
198
115
90.5
1 2 3 4
IP: FLAG
WCE
hTERT-(Ub)n
WT Rin
g+ + + +
Hdm2
HA-Ub
FLAG-hTERT
MG132
- + + +
+ + + +
- -
WB: HA
WB: Hdm2
WB: FLAG
WB: FLAG
d
115
198
90.5
1 2 3 4
IP: FLAG
WCE
hTERT-(Ub)n
WB: HA
WB: Hdm2
WB: FLAG
WB: FLAG
WT
C464A
+ + + +
Hdm2
HA-Ub
FLAG-hTERT
MG132
- + + +
+ + + +
- -
WB: Hdm2
WB: HA
WCE IP: HA
1 2 3 4
Hdm2 C464A
hTERT-HA
+ + + +
- + - +
- wtC464A
+ + +
1 2 3
WB: Hdm2
WB: HA
WB: GFP
Hdm2 C464A
hTERT-HA
b
c
Supplementary Figure S2. Oh et al.
Supplementary Figure S3. Oh et al.
hTERT-(Ub)n
WCE
PD: Ni-NTA
1 2 3 4 5WB: Myc
WB: HA
WB: HA
+ + + + +
Myc-Hdm2s
His-Ub
hTERT-HA
MG132
- -
- + + + +
+ + + + +
198
115
115
61.5
90.5 1-301
294-491
WT
1-30
129
4-49
1
WT
a
Myc-Hdm2 294-
489
PCNC
wt
1-30
1
hTERT - + + + +
IC (36bp)
50 bp
b
a
350-1132
hTERT1132
1-350
1-542
595-1132
595 9461RT
b
Supplementary Figure S4. Oh et al.
hTERT-HA
Hdm2
1-542
- +
595-1132
- +
1-350
- +
350-1132
- +
hTERT
- +
11590.5
46.261.5
37.8
11590.5
46.2
61.5
37.8
11590.5
46.2
61.5
37.8
11590.5
46.2
61.5
37.8
IP: Hdm2
WCE
Hdm2
Hdm2
hTERT
hTERT
a cb
SC-7PCSC-1
0
Mock
1
d
NC
e
Mock
2
WB: FLAG
WB: HA
1 2 3 4
FLAG-Hdm2
hTERT-HA
WCE IP: FLAG
-+- +
+++ +
FLAG-Hdm2
hTERT-HA
GFP
- +
+
+ +
FLAG-Hdm2
hTERT-HA
GFP
+
115
198kDa
90.5
WB: HA
WB: Hdm2
WB: MycWCE
1 2 3
hTERT-(Ub)n
WB: Myc
IP: Myc
+ + +
FLAG-Hdm2
HA-Ub
Myc-hTERT
MG132
- - +
- + +
+ + +
Mock
-1
SC-7SC-1
0
Mock
-2
FLAG-Hdm2
Actin
IC (36bp)
50 bp
1 2
1 2 3 4
1 2 3 4 5 6
Supplementary Figure S5. Oh et al.
a cb
Supplementary Figure S6. Oh et al.
M1M1
M1
R1FLAG-negative
R2FLAG-positive
R1FLAG-negative
R2FLAG-positive
R1HA-negative
R2HA-positive