PATENT ABSTRACTS

DNA fraction having a structural gene which codes human interleukin 2 peptide are linked to each other. This novel DNA makes it possible to produce a polypeptide in which the two peptides arelinked to each other by transforming host microorganism with the DNA and culturing the transformed microorganism.

8504663

M E T H O D O F D E T E C T I N G N U C L E I C A C I D S E Q U E N C E S

James L HARTLEY. Mark S BERNINGER as- signed to LIFE TECHNOLOGIES INC;

A process for detecting specific nucleotide sequences, called targets, in which a special DNA probe molecule, called a probe-vector, is capable of transforming bacteria if and only if it is held in a circular configuration by base pairing to a target nucleic acid, said transformation resulting in the detection of a phenotype specified by the probe-vector, said detection establishing the presence, absence, or quantity of the target; and a probe-vector molecule for per- forming the process.

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Mary Louise SKOTN1CKI. Antek Henryk SKOTNICKI, Adrian John GIBBS, 54 A'Beckett Street, Watson, ACT 2600, Australia assigned to THE AUSTRALIAN NATIONAL UNIVERSITY

A method of direct insertion of double-stranded RNA into a cloning vector consisting of double- stranded DNA, the method comprising: a) isolating the dsRNA from a source of interest: b) if required, decapping the dsRNA: and c) directly ligating the dsRna to the dsDNA of the appropriate cloning vector. This method allows dsRNA to be cloned directly, thus eliminating the traditional step of first making a comple- mentary DNA copy of the RNA molecule. Using this method, it is possible to construct probes for the detection of virus diseases in plants and animals. The method can also be used to produce new plant strains which are resistant to a particular virus, or to provide immunity to animals to a specific virus.

8504899

M E T H O D S A N D V E C T O R S F O R T R A N S F O R M A T I O N O F P L A N T

C E L L S

8504673

N O V E L D N A A N D I T S U S E

Masaharu SENOO, Haruo ONDA, Koichi IGARASHL D73-106, 18 Tsukumodai 5- chome. Suita-shi, Osaka 565, Japan assigned to TAKEDA CHEMICAL INDUSTRIES LTD:

A novel DNA is prepared in which a DNA frac- tion having a structural gene which code a pep- tide containing an antibody recognition site and a DNA fraction having a structural gene which code human interleukin 2 peptide are linked to each other. This novel DNA makes it possible to produce a polypeptide in which the two peptides are linked to each other by transforming host microorganism with the DNA and culturing the transformed microorganism.

David H GELFAND, Kenneth A BARTON as- signed to CETUS CORPORATION: AGRACETUS

Vectors and methods suitable for both direct and Agrobacterium)-mediated transformation of plants. The vectors comprise easily man- ipulated expression cassettes and optionally con- tain border sequences associated with Agrobacterium) plasmid DNA transfer. By using an intermediate carrier vector which con- tains the requisite expression control sequences in accessible form, a wide variety of single and multiple cassette vectors can be constructed.

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R O T A V I R U S

8504898

AN I M P R O V E D M E T H O D O F C L O N I N G D O U B L E - S T R A N D E D

RNA, A N D ITS U S E IN T H E P R O D U C T I O N O F V I R A L G E N E

C L O N E S

lan Hamilton HOLMES, Michael Leigh DYALL-SMITH. 13 Avenue Athol, Canter- bury. VIC 3126, Australia assigned to THE UNIVERSITY OF MELBOURNE

A material being a dsRNA gene segment coding Ibr the major outer capsid glycoprotein of a rolavirus.