8 - royal society of chemistry · figure si-10: (a) uv-visible absorbance spectra of n-aunps with...
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Figure SI-1
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Figure SI-1 Figure S1a Molecular structure of Naringin with side chains marked as A and B. Figure S1b UV- Vis scan of absorbance versus wavelength of pure Naringin, [N] = 0.5mM in a buffer of pH 9.
Electronic Supplementary Material (ESI) for RSC Advances.This journal is © The Royal Society of Chemistry 2016
Figure SI-2
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Figure SI-2 UV-Visible spectra of HAuCl4 +Naringin mixture in a buffer of pH 9 with [HAuCl4] = 0.4mM and [N] = 0.05 (2a), 0.2mM (2b) at 40oC. UV-Visible spectra of HAuCl4 +Naringin mixture with [N] = 0.5mM and [Au] = 0.2 (2c), 0.6 (2d), 1.2mM (2e) at 40oC. Blank means no Naringin is added. All the inset shows the SPR peak and colour of AuNPs in each case.
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Figure SI-3
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Figure SI-3: Absorbance versus wavelength scans of [AuCl4] = 0.4mM + [N] = 0.5mM at various temperatures. (a) 40oC (b) 50oC (c) 60oC (d) 70oC over a time interval of 120 minutes.
Figure SI-4
Figure SI-4: FTIR spectra of (a) Pure Naringin (b) enlarged view of peaks of Pure Naringin from 400-1800 cm-1 (c) Naringin stabilized AuNPs.
Figure SI-5
Figure SI-5: Image of gel-electrophoresis of N-AuNPs.
Figure SI-6
Figure SI-6: XRD spectrum illustrating the crystalline nature of N-AuNPs.
Figure SI-7
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Figure SI-7: Minimum energy structure of (a) [C10H7O2] adsorbed on Au13 cluster and (b) [C6H5O]- adsorbed on Au13 cluster (Yellow spheres represent Au, Black C, white H and Red O.
Figure SI-8
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Figure SI-8: NTOs for the excitations which make up the dominant peak in the Vis region (a) Hole part and (b) Particle part of [C6H5O]--Au13. Phases of the orbitals are indicated in red and blue.
Figure SI-9
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Figure SI-9: Minimum energy structure of (a) [C10H7O2] adsorbed on Au32 cluster and (b) [C6H5O]-
adsorbed on Au32 cluster.
Figure SI-10
Figure SI-10: (a) UV-Visible absorbance spectra of N-AuNPs with various metal ions. Inset shows a photograph representing the N-AuNPs solution and its response towards Al3+. (b) The bars represent the calorimetric response of N-AuNPs with various metal ions in terms of change in absorbance ∆A with respect to the blank i.e. N-AuNPs. (c) The bars represent the calorimetric response of N-AuNPs with various metal ions in terms of change in wavelength ∆λ with respect to the blank i.e. N-AuNPs.
Figure SI-11
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Figure SI-11: (a) UV-Visible spectrum of N-AuNPs as a function of various concentrations of Al3+ ions (b) plot of absorption ratios A640/A520 of N-AuNPs versus Al3+ concentration.
Figure SI-12
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Blood+water[N-AuNPs] = 50 µg/ml[N-AuNPs] = 25 µg/mlBlood+PBS
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Figure SI-12: (a) Heme Absorption Spectra with different concentrations of N-AuNPs i.e. [N-AuNPs] = 25 and 50 µg/ml. (b) Enlarged view of Absorption peaks of heme from 500-600 nm.
Figure SI-13
MCF Control N-AuNPs 100 µg/ml
T47D Control N-AuNPs 100 µg/ml
MDAMB-231 Control N-AuNPs 100 µg/ml
Figure SI-13: Laser Scanning microscope images of cell lines (a, c, and e) represents the morphology in untreated MCF-7, T47D , and MDAMB controls (b, d, and f) represents the changes in morphology in MCF-7, T47D, and MDAMB in the presence of [N-AuNPs] = 100 µg/ml.