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201 | Appendix - I

8. Appendix - I

Formulation of cultured media

i. Czapek-Dox broth (CDB) Medium

Sucrose 30.0 g

Sodium nitrate 3.0 g

Dipotassium phosphate 1.0 g

Magnesium sulphate 0.5 g

Potassium chloride 0.5 g

Ferrous sulphate 0.01 g

Distilled water 1.0 lit

Final pH (at 25°C) 7.3±0.2

ii. Malt extract agar (MEA) Medium

Malt extract 30.0

Mycological peptone 5.0

Agar 15.0

Distilled water 1.0 lit

Final pH (at 25°C) 5.4±0.2

iii. Potato dextrose agar (PDA) Medium

Potatoes, infusion from 200.0 g

Dextrose 20.0 g

Agar 15.0 g

Distilled water 1.0 lit

Final pH ( at 25°C) 5.6±0.2

iv. CarboxyMethyl-Cellulose (CMC) Medium

CarboxyMethyl-Cellulose 15.0 g

NH4NO3 1.0 g

KH2PO4 monobasic 1.0 g

MgSO4.7H2O 0.5 g

Yeast extract 1.0 g

Distilled water 1.0 lit

v. Formulation and preparation of Corn Meal Sand media

Sand 70.0 kg

Garden soil 30.0 kg

These two were mixed thoroughly in a container and 6.0 kg of

this mixture were replaced with Crushed corn (~ 2 to 5 mm) and

again mixed thoroughly. The new mixture was filled in small

container of ~ 10 kg capacity and autoclaved three times at

121ºC for 6 hrs per day.

202 | Appendix - II

9. Appendix - II

Fungal culture submitted to NTCC, Forest Pathology Division,

FRI, Dehradun, Uttarakhand

S. No Fungal species Allotted NTCC

number

1. Armillaria mellea 1227

2. Auricularia auricula 1228

3. Chaetomium globosum 1238

4. Lycoperdon pyriforme 1229

5. Mucor varians 1237

6. Polyporus arcularius 1230

7. Pleurotus ostreatus 1232

8. Polyporus obtusus = Spongipellis

unicolor 1233

9. Sparassis crispa 1234

10. Scytalidium lignicola 1239

203 | Appendix – III

10. Appendix – III

Paper presentations and publications:

Rajendra Prasad, V.K. Varshney and N.S.K. Harsh, 2012.

Evaluation of Antioxidants Capacity and Total Phenolic

Contents of Submerged Cultures of Some edible mushrooms.

Indo-US Workshop, Green Chemistry for Environment and

Sustainable Developments, Dept. of Chemistry, HNBGU,

Uttarakhand, India. 11-13 March, 2012.

Rajendra Prasad, V.K. Varshney and N.S.K. Harsh, 2012.

Evaluation of Antioxidant Capacity, Total Phenolic Contents and

Antimicrobial Activity of Endophytic Fungus Chaetomium

globosum. 7th Uttarakhand State Science and Technology

Congress-2012. 21-23 November, 2012.

Rajendra Prasad, N.S.K. Harsh and V.K. Varshney, 2014.

Comparative study of TPCs and antioxigenic capacity of fruiting

bodies and their mycelial cultures of some mushrooms species

(communicated to International Journal of Medicinal Mushroom).

Rajendra Prasad, V.K. Varshney and. N.S.K. Harsh, 2014.

Variation in TPCs and antioxidant capacity of fruiting body and

mycelial biomass of Ganoderma lucidum collected from nine tree

species (communicated to Journal of Biologically Active Products

from Nature).