7/29/2019 7 . 5 M Lithium Chloride Precipitation Solution_Unknown_1832_Unknown

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Ambion, Inc. 2130 Woodward St. Austin, TX USA 78744-1832 International Tel: +1 512 651 0200

US Toll-free Tel: 800 888 8804 E-mail: techserv@ambion.com www.ambion.comwww.ambion.com/techlib/spec/sp_9480.pdf

Catalog #: 9480 100 ml

Appearance: Clear Liquid

Description: 7.5 M Lithium Chloride, 50 mM EDTA, pH 8.0

Storage Conditions: Store at room temperature.

Quality Control: Ambions 7.5 M Lithium Chloride solution is tested in the following quality control assays

before and after final packaging. The product is filtered through a 0.2 m filter into the final

package and autoclaved.

Non-Specific Endonuclease/Nickase Activity: None detected after a 14-16 hour incubation

of 0.75 M LiCl with 300 ng of supercoiled plasmid DNA, analyzed on an agarose gel.

Exonuclease Activity: None detected after a 14-16 hour incubation of 0.75 M LiCl with 40

ng of32

P-labeled Sau3A fragments of pUC19, analyzed by PAGE.

RNase Activity: None detected after a 14-16 hour incubation of 0.75 M LiCl with 25 ng of32

P-labeled RNA, analyzed by PAGE.

USER INFORMATION:

This solution is ready for use and requires no extra preparation. Though not necessary, this solution can be re-

autoclaved. If precipitants form, warm solution to 37C to resuspend.

LiCl precipitation is very useful following RNA isolation or in vitro transcription because RNA is efficiently precipitated,

while free NTPs, RNA molecules smaller than 200 bases, protein, and DNA are very inefficiently precipitated or are not

precipitated at all.

To precipitate RNA in a solution with 400 ng/l or greater RNA concentration, bring the final concentration of LiCl in the

RNA solution to 2.5 M, chill the solution at -20C for 30 minutes, and centrifuge at top speed in a microcentrifuge for 15

minutes. Discard the supernatant and wash the pellet with ice-cold 70% EtOH to remove residual salt, then resuspend

in nuclease-free H2O or TE.

7.5 M Lithium ChloridePrecipitation Solution

9480

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All products sold by Ambion are intended for research use only unless otherwise indicated. This product is not intended

for diagnostic or drug purposes.

Warranty and Liability: Ambion is committed to providing the highest quality reagents at competitive prices. Ambion warrants that the products meet or exceed the

performance standards described in the product specification sheets. If you are not completely satisfied with any product, our policy is to replace the product or credit

the full purchase price and delivery charge. No other warranties of any kind, expressed or implied are provided by Ambion. Ambion's liability shall not exceed the

purchase price of the product. Ambion shall have no liability for direct, indirect, consequential or incidental damages arising from the use, results of use, or inability to

use its Products.0999