5190872 immobilized alcohol utidase for use in an alcohol measuring apparatus
TRANSCRIPT
PATENT ABSTRACTS
second enzyme, and the leash for the second en- zyme only is cleaved in the system by the first en- zyme. These two materials are connected in series, and upon application of the second en- zyme to the first enzyme-support conjugate, fol- lowed by application of the released first enzyme to the second enzyme-support conjugate material, ultimately a large amount of released active second enzyme is produced. Also dis- closed are molecular conjugates of enzyme material on supports, required for the enzyme amplification.
5190872
I M M O B I L I Z E D A L C O H O L U T I D A S E F O R U S E I N A N A L C O H O L M E A S U R I N G
A P P A R A T U S
Yoshio Hashizume, Akio Kariyone, Ryuzo Hayashi, Minako Oka, assigned to Kanzaki Paper Mfg Co Ltd
Alcohol oxidase is immobilized by bonding an aminosilane coupling agent to a carrier, bonding a multifunctional aldehyde such as glutaral- dehyde to an amino group of the aminosilane, washing the carrier to remove free multifnnc- tional aldehyde and bonding alcohol oxidase to the multifunctional aldehyde bonded to the aminosilane. Washing to remove free multifunc- tional aldehyde enables immobilizing a one molecule thickness of alcobol oxidase on the car- rier since free multifunctional aldehyde is not present to cross-link alcohol oxidase molecules together. A thin layer of alcohol oxidase is ad- vantageous when assaying for alcohol since a thin layer does not retain hydrogen peroxide that can deactivate alcohol oxidase. The carrier pre- ferably contains hydroxyl groups and is porous, and can be a silicate-containing carrier such as diatomaceous earth or fire brick. The im- mobilized alcohol oxidase is used in a column as part of an apparatus for measuring alcohol or as part of an apparatus containing immobilized glucose oxidase for measuring glucose and alcohol.
5192415
B I O S E N S O R U T I L I Z I N G E N Z Y M E A N D A M E T H O D F O R
P R O D U C I N G T H E S A M E
Toshihiko Yoshioka, Shiro Nankai, Osaka, Japan assigned to Matsushita Electric Industrial Co Ltd
191
The biosensor of the present invention quan- tities a substrate contained in a sample liquid by reducing electron acceptors using electron gene- rated in a reaction oftbe substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically. The bio- sensor has an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode, a reaction layer including the enzyme provided on the elec- trode system and a hydrogen ion concentration control layer, and the reaction layer is in contact with the electrode system. According to the pre- sent invention, the hydrogen ion concentration in the sample liquid can be made most ap- propriate in accordance with the type of the enzyme contained in the reaction layer, without the adjustment of the hydrogen ion concentra- tion in the sample liquid beforehand. Thus, the specific substrate contained in the sample liquid can be easily quantified with accuracy and speed.
5192657
S T A B I L I Z E D P R O T E O L Y T I C S O L U T I O N A N D R E A G E N T K I T
Janice Jakway, Dennis Mochnal assigned to Or- tho Diagnostic Systems Inc
A stabilized proteolytic solution has been developed which can be used to treat selected cells, especially red blood cells. A preferred stabilized solution of the invention comprises about 0.1% to about 0.3% ficin in a citrate- buffered saline solution containing mannitol, L- cysteine, dithiothrietol in an active concentration of less than about 10 raM, and EDTA. A kit for qualitatively identifying unex- pected blood group antibodies comprising a series of untreated and ficin-treated red blood cells, a stabilized ficin reagent, a red blood cell diluent and an enzyme control is also provided.
5192677
A L K A L I A N D H E A T S T A B L E P R O T E A S E F R O M
T H E R M O M O N O S P O R A F U S C A
John E Kinsella, Todd W Gusek, David B Wilson, Osaka, assigned to CorneU Research Foundation Inc
An exocellular protease from Thermo- monospora fusca YX and a process for pro- ducing the protease which has the following physicochemical properties: (1) Molecular mass: