538 PATENT ABSTRACTS

expression in various microorganisms of the gene, known as cryC, which codes for this toxin, and for related novel insecticide compositions and methods for their use.

5024944

T R A N S F O R M A T I O N , S O M A T I C E M B R Y O G E N E S I S A N D W H O L E

P L A N T R E G E N E R A T I O N M E T H O D F O R G L Y C I N E S P E C I E S

Glenn B Collins, David F Hildebrand, Paul Laz- zeri, Thomas Adams, Wayne A Parrott, Lynn M Hartweck assigned to Lubrizol Genetics lnc; The University of Kentucky Research Founda- tion Inc

A method for somatic embryogcnesis of soy- bean, (Glycine max), Glycine soja and other Glycine species is provided using immature cotyledon tissue, preferably with the embryonic axis removed, comprising culturing said tissue on a medium containing auxin, preferably NAA at a concentration of at least about 15 mg/I. A further method for such somatic embryogenesis is provided wherein the culture medium contains a synergistically acting lowered carbohydrate and auxin concentration. Particularly em- bryogenic cells of such tissue are identified and improved maceration methods for contacting such cells with regeneration and transformation media are disclosed. Methods for transforming somatic tissue from soybean and other Glycine species are also provided. Whole, fertile, trans- formed plants are obtained.

5026547

C O M P O U N D H A V I N G I N S E C T I C I D A L A C T I V I T Y A N D I N S E C T I C I D E C O M P O S I T I O N

C O N T A I N I N G T H E S A M E

Kei-ichi Imamura, Shuichi Gomi, Michiaki lwata, Shinji Miyadoh, Masaru Shimura, Takashi Shomura, Masaji Sezaki, Shigeharu In- oye, Kanagawa, Japan assigned to Meiji Seika Kaisha Ltd

A novel compound having an intense in- secticidal activity and a process for producing the same which comprises culturing a strain belonging to the genus Humicola and isolating the compound from the culture.

5028253

P R O C E S S F O R C O N T R O L L I N G U N D E S I R A B L E V E G E T A T I O N

W I T H C O L L E T O T R I C H U M C O C C O D E S S P O R E S

Alan K Watson, Alan R Gotlieb, Pincourt, VT, Canada assigned to The Royal Institution for the Advancement of Learning (McGill Univ ); The Univ of Vermont and State Agriculturual Colleg

There is disclosed a process for controlling un- desirable vegetation, especially velvetleaf by ap- plying Colletotrichum coccodes spores onto the undesirable vegetation or onto the situs of the undesirable vegetation. The composition in- cluding the fungus spores in an agricultural car- rier as well as the growing of the fungus on solid or liquid media are also disclosed. The spores germinate, infect the undesirable vegetation and result in a pathogenic disease which kills or reduces the growth of this undesirable plant.

5028540

A V I A N I M M U N O G L O B U L I N P R O D U C I N G C E L L L I N E S

Eric H Humphries assigned to The Board of Re- gents The University of Texas System

The present invention involves a method for pre- paring antibody-producing chicken cell clones. This method comprises a series of steps including initially immunizing a first chicken with a desired antigen. A population of antibody-producing lymphocytes from bursa or spleen of the first chicken is separated. The antibody-producing lymphocyte population is then infected with helper-free reticuloendotheliosis virus REV-T or helper-free reticuloendotheliosis virus REV-T and CSV (preferably REV-T(CSV) and trans- planted into a second chicken, the second chicken having been pretreated to remove nor- mal B cells. The transplanted lymphocytes to proliferate in the .~ond chicken, preferably for a period of at least about two weeks. The lymphocytes from spleen, bursa or peripheral blood of the second chicken are isolated and plated out, for example in microtiter plates. Cell clones producing antibody such as IgG or IgM directed against the desired antigen are then selected. The isolation step most preferably in- volves cell culturing in the microtitcr plates for a period of at least about one week.