PATENT ABSTRACTS 483

5001064

P H O S P H A T I D Y L I N O S I T O L 4 - K I N A S E

Thomas Deuel assigned to Washington Univer- sity

A novel phosphatidylinositol 4-kinase isolated from bovine uterus having the lbllowing charac- teristics: (a) molecular weight of about 55 kDa as determined by sodium dodecylsulfate poly- acrylamide gel electrophoresis; (b) Km of about 18 muM for ATP; (c) Km of about 22 mug,'ml for phosphatidylinositol; (d) pH optimum of ab- out 6.0 to 7.0; (e) activated by Mg2+: (f) in- hibited by Ca2 +; (g) utilizes ATP and 2"-dioxy- ATP as phospboryl donors and specifically phosphorylates phosphatidylinositol on the 4- position; and (h) essentially free of phosphatidylinositol 4-phosphate 5-kinase ac- tivity determined by treatment with phorphoryl donors ADP. GTP, ITP or CTP.

5 ~ 1 0 ~

M E T H O D F O R C A R R Y I N G O U T S P A R G E D R E A C T I O N

D O Hitzman assigned to Phillips Petroleum Company

Process for carrying out a sparged reaction, in- cluding providing one or a plurality of vertically- disposed reaction tubes, introducing a sparging gas under pressure into the bottom of the tube, separating and withdrawing the sparging gas from the top of the tube and passing the separated sparging gas in indirect heat exchange with at least part of the outside surface of the tube. The process is particularly suitable for car- rying out an exothermic reaction, such as a fer- mentation reaction for the production of single cell protein, in which the bottom of a plurality of reaction tubes are disposed in a water bath, the withdrawn sparging gas is sprayed across the tops of the tubes, preferably after reducing the pressure to cool the same, and water is with- drawn from the bath and passed downwardly along the outside of the tubes.

A continuous hybridoma cell line, designated 7H8, which secretes a monoclonal antibody (MAb 7H8) of the lgM class and which binds to a protein present in species of the genus Plas- modium. MAb 7H8 also recognizes an antigen (antigen Pf93) unique to P. falciparum Methods of isolating the substantially pure antigen using the antibody of the present invention are dis- closed. MAb 7H8 is well suited foruse in immunometric assays. Preferred is a two-sited assay developed with MAb 7H8. Anti-idiotypic and anti anti-idiotypic antibodies to MAb 7H8 are disclosed. The antibodies and antigens of the present invention are useful for immuno- diagnostic and immunotherapeutic treatment of malarial diseases of man and animals.

5002881

P R O C E S S F O R T H E F E R M E N T A T I V E P R E P A R A T I O N

O F O R G A N I C A C I D S

Joannes G M Van Nispen, Ronald Jonker, Ber- gen op Zoom, Netherlands assigned to Cooperatieve Vereniging Suiker Unie U A

The present invention relates to a process for the fermentative preparation of lactic acid, by al- lowing a sterilized growth medium to be con- tinuously fermented in a reaction vessel by a culture of bacilli of the genus Bacillus, which form lactic acid, keeping the pH of the reaction mixture at 3 to 9 with an aqueous solution of XOH (where X is NH4 or a metal whose hydro- xide and whose salt of lactic acid arc water- soluble), subjecting the fermented reaction mixture to an ultrafiltration, recycling the retch- tare to the reaction vessel, and concentrating the permeate and subsequently subjecting it to an electrodialysis wherein bipolar membranes are used, in which process the lactate is decomposed into the lactic acid and XOH.

5002889

R E A C T I O N W E L L S H A P E F O R A M I C R O W E L L T R A Y

5001225

M O N O C L O N A L A N T I B O D I E S T O A P A N - M A L A R I A L A N T I G E N

Diane W Taylor assigned to Georgetown University

Gerald L Klein assigned to Genetic Systems Corporation

A microwell for enzyme-linked immunosorbent as~ys has a concavely curved, circumferential sidewall, a flat optical bottom and a top lip, in- cluding smooth transitions between the top lip.