E n z y m e s a n d E n z y m e S y s t e m s

4962024

S I G N A L E N H A N C E M E N T I N A S S A Y F O R A N E N Z Y M E

Thomas H Schulte assigned to Becton Dickinson and Company

A method for assay for an unknown enzyme suspected to be present in a liquid includes signal amplification by use of a second enzyme and a blocked modulator for the second enzyme. Unknown enzyme in the liquid removes a blocking group from the blocked modulator. The resulting modulator activates or inhibits the second enzyme which catalyzes an indicator reaction in which a substrate is converted to a product. The presence or absence of the unknown enzyme in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the indicator reaction. The concentration of the enzyme in the sample may be determined by the measurement of the signal. The invention includes a kit of materials useful for performing the method of the inven- tion. The method involves the hydrolyzing of a blocked fluoroketone inhibitor to facilitate the analytical method.

4963368

O X I D O R E D U C T A S E E N Z Y M E S T A B I L I Z E D H I G H L Y

U N S A T U R A T E D F A T T Y A C I D S A N D D E R I V A T I V E S O F S U C H

A C I D S

Richard L Antrim, Norman Lloyd, James Taylor assigned to Nabisco Brands Inc

Highly unsaturated fatty acid compounds, and derivatives thereof, are stabilized against oxida-

tion with a water activated oxidoreductase en- zyme. The fatty acid containing component is preferably microencapsulated in a wall member which comprises the enzyme.

4963469

E N Z Y M A T I C A L L Y I N A C T I V E , I M M U N O L O G I C A L L Y - A C T I V E

B E T A - G A L A C T O S I D A S E M U E T I N , P R O C E S S F O R M A K I N G A N D

U S E S T H E R E O F

Ral Mattes, Helmu Lenz, Werner Stock, Stutt- gart, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH

The present invention provides an enzymatic, ally-inactive, immunologic.ally-active beta-galactosidase mutein, wherein, in the re- gion between the amino acids 430 and 550, at least one amino acid of the natural sequence is changed to another amino acid and the en- zymatic activity does not amount to more than 1%, referred to the native enzyme. The present invention also provides a process for the produc- tion of this mutein. Furthermore, the present in- vention is concerned with the use of this mutein in the immunological determination of serum proteins by the enzyme immunoassay principle.

4963479

R E A G E N T S Y S T E M F O R A N A L P H A - A M Y L A S E A S S A Y C O N T A I N I N G A R O M A T I C

S U B S T I T U T E D G L Y C O S I D E

Rodrigo G Chavez, Harold David, Ernest K Metzner, Gerald F Sigler, Emily S Winn-Deen assigned to Hoechst Celanese Corporation

103

104 PATENT ABSTRACTS

An aromatic substituted glycoside is disclosed of the formula See Patent for Chemical Structure wherein the configuration of the substituted -OR on the anomeric carbon is alpha, n is an integer of 0 to 1, and R is a substituted aromatic radical selected from the group See Patent for Chemical Structure (a) R3R2R4and (b) R5R6 (c) where R1 through R6 are independently halogen, NO2, SO3H, See Patent for Chemical Structure where R7 is lower alkyl; and includes its stereoisomers, optical isomers and geometric isomers and mix- tures of the foregoing isomers. These substrates are useful as direct substrates for alpha- amylases. A process for the preparation of the substrates and related substances is also described.

pregnating a solution of the water-soluble poly- mer into the porous layer of the ultra.filtration membrane under a pressure of 0.1 to 1.0 kg/cm2, washing the porous layer, passing a solution of a crosslinking agent through the porous layer un- der a pressure within the same pressure range as above to crosslink the water-soluble polymer, removing the non-crosslinked water-soluble polymer by back washing from the dense layer of the membrane, and then passing a solution of the enzyme under a pressure within the same pre- ssure range as above through the porous layer, whereby the enzyme is covalently bonded to the membrane through the functional groups of the polymer.

4963492

M E T H O D F O R T H E E N Z Y M A T I C R A C E M A T E R E S O L U T I O N O F

R A C E M I C A L C O H O L S W I T H / I N V I N Y L E S T E R S BY

T R A N S E S T E R I F I C A T I O N

Reinhold Keller, Wolfgang Holla, Gerd Fulling, Bad Soden am Taunus, Federal Republic Of Germany assigned to Hoechst Aktiengesel- lschaft

Racemic alcohols can be resolved in good yield and with high enantiomeric purity in the pre- sence of vinyl esters with the aid of lipases from pig pancreas, pig liver and microorganisms.

4963494

E N Z Y M E I M M O B I L I Z A T I O N I N A N A N I S O T R O P I C

U L T R A F I L T R A T I O N M E M B R A N E

Ken Hibino, Takeshi Okada, Kazuo Nakao, Yuko Sahashi, Osaka, Japan assigned to Nitto Electric Industrial Co Ltd

An enzyme immobilized membrane comprising an anisotropic ultrafiltration membrane having a porous layer and a dense layer, and an enzyme immobilized therein is disclosed. The porous layer of the ultrafiltration membrane retains a water-soluble polymer having at least two func- tional groups in the crosslinked state, and the en- zyme is covalently bonded to the membrane through the functional groups of the polymer. Preferably, the membrane is prepared from poly- sulfone. The enzyme immobilized membrane is produced by a process which comprises im-

4965012

W A T E R I N S O L U B L E E N C A P S U L A T E D E N Z Y M E S

P R O T E C T E D A G A I N S T D E A C T I V A T I O N BY H A L O G E N

B L E A C H E S

Keith E Olson

A composition capable of releasing active en- zyme into an aqueous, active chlorine containing media which in a first aspect comprises an en- zyme core encapsulated with an initial coating of a time-release substance, a first coating of a bleach-neutralizing substance and a second coating of a time-release substance. In a second aspect, the composition comprises an enzyme encapsulated in a time-release substance designed to delay release of the enzyme in dis- solution for a first-time delay, and a bleach- neutralizing substance, present as either a core material and/or a first coating on a diluent core, which is encapsulated in a time-release substance designed to delay release of the bleach- neutralizing substance into solution for a second-time delay; the first-time delay being longer than the second-time delay so that the bleach-neutralizing substance will be released and completely neutralize all active chlorine pre- sent in the solution before the enzyme is released. In a third aspect, the composition comprises an enzyme core encapsulated with a time-release substance, a diluent core encapsulated with a first coating of a bleach-neutralizing substance and a second coating of a time-release substance, and a bleach-neutralizing substance core encap- sulated with a time-release substance. The inven- tion further includes a cleaning composition which is particularly effective in warewashing which comprises one of the encapsulated enzyme-containing compositions described ab-