PATENT ABSTRACTS 437

and without using a number of nozzles of smaller size.

4829002

SYSTEM FOR METERING NUTRIENT MEDIA TO CELL

CULTURE CONTAINERS AND METHOD

Martha Pattillo, David V Bacehowski, Arnold Bilstad, Herbert Cullis, T Michael Dennehey, James W Yang, William C Brown assigned to Baxter International Inc

A system for metering nutrient media to cell cul- ture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile con- nectors may be used for essentially sterile condi- tions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The Row conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture con- tainer, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.

4829003

PYROELECTRIC ENZYME DETECTOR

Lawrence H Arney

A pyroelectric sensor is provided for detecting enzymes or enzymatic substrates in a liquid or gas stream. The sensor contains a laminate for- med of a pyroelectric material sandwiched bet- ween upper and lower conducting means to act as electrodes. An electronic circuit means is con- nected to the conducting means to measure ther- mally induced electric current and an enzyme or enzymatic substrate is immobilized on a surface of one of the conducting means. The laminate is supported by a support means and a plate means rests on an upper surface of the supported laminate. The plate means has a longitudinal

flow channel communicating with the upper sur- face for flow of a gas of liquid, and a recess for accommodating the electronic circuit means. In- let and outlet means pass through the plate means and communicate with the flow channel,

4829004

ROLLER BOTTLE SYSTEM

James Varani, David Solomon, William J Hil- legas, David L Melmoth assigned to The Univer- sity of Michigan; Solohill Engineering I

An insert for a roller bottle of the type used in cell culture for vaccine manufacture is formed of a polymeric resilient material whereby it can be bent or rolled so that it can pass through the opening of the roller bottle. Once the insert is in- side the bottle, it is released so that its resilient characteristic causes it to unbend or unroll. The insert is wider than the diameter of the roller bot- tle, and therefore it is urged against the interior wall of the roller bottle. Apertures and/or not- ches are provided in the insert to increase the turbulence of a fluid and to decrease the Auidic shear forces in the roller bottle as it is rotated in conventional operation, A significant increase in productivity is achieved because particles, which may be microcarrier beads, are maintained in suspension, and prevented from sinking to the bottom of the fluid medium where cells cannot grow. The roller bottle system is thereby trans- formed into a microcarrier suspension culture system.

4829005

SEDIMENTATION FILTRATION MICROORGANISM GROWTH

CULTURE SYSTEM

Michael P Friedman, Jame Jablonsky, Ronald F Schell

Apparatus for isolating and identifying micro- organisms in blood or the like which comprises a tubular collection vessel which is sealed at both ends but includes means at one end for permit- ting the removal of fluid therein by the applica- tion of vacuum when the seal at that end is broken, a multisection filtration unit comprising a first section provided with means for receiving said one end of the collection vessel, means in said section for breaking the seal in said one end of the vessel when it is received by said first sec- tion, a second section of said filtration unit